The invention discloses a plant molluscicide as well as a preparation method and application thereof. The molluscicide consists of the following components in percentage by weight: 1-40% of a mother medicine and 60-99% of an auxiliary agent. The preparation method comprises the following steps: (1) removing grease of tea seed meal; (2) adding tea seed meal powder into a 5-18% sodium hydroxide solution of which the weight is 5-6 times that of the tea seed meal powder, soaking for 4-5 hours at 85-105 DEG C to obtain a soaking solution, then adding alkaline protease which accounts for 5-20% of the soaking solution in weight into the soaking solution, soaking for 10-14 hours at 55-75 DEG C, filtering to obtain a filtrate and a filter cake, and drying the filter cake to obtain the auxiliary agent; (3) neutralizing the filtrate by using 5-10% hydrochloric acid until precipitates cannot be separated out any more, centrifuging, removing supernatant liquid to obtain lower-layer precipitates, and drying the lower-layer precipitates to obtain the mother medicine; and (4) crushing the mother medicine and the auxiliary agent into 100-300 meshes, and then uniformly mixing. The molluscicide disclosed by the invention has the advantages of high efficiency, low toxicity, environmental protection, easiness in degradation, low cost, safety, convenience and the like. The method disclosed by the invention is simple, feasible, simple in technological process, and slight in pollution to the environment.