5 results about "Oncomelania" patented technology

This invention relates to a zero-shot Oncomelania snail identification method and system based on a structured knowledge base and attention guidance, belonging to the field of image processing technology. The method includes: acquiring original images of Oncomelania snails; generating an attention map using a pre-trained model to locate and crop discriminative regions; constructing a structured Oncomelania snail knowledge base and guiding a large language model to generate fine-grained text descriptions; extracting image and text features separately, calculating region weights and text weights; and determining the snail species through weighted image-text similarity matching. This invention achieves zero-shot identification without annotating image data, effectively suppresses interference from complex backgrounds, significantly reduces data and annotation costs, and improves identification efficiency and robustness, making it suitable for low-cost Oncomelania snail monitoring in field environments.

This invention discloses a self-cleaning, anti-interference snail crushing device and its usage method for microscopic examination. The device includes a main body, a flat crushing unit, a snail placement unit, a pressure plate cleaning unit, and a snail placement plate detachably installed on the upper surface of the snail placement unit for placing and positioning snails. It employs automated pressure application technology, solving the technical problem that manual crushing methods inevitably cause uneven stress on the snails and varying degrees of crushing when handling a large number of snails for microscopic examination, thus affecting the quality of subsequent microscopic examinations. Furthermore, by integrating the pressure plate cleaning unit into the device, the pressure plate cleaning scheme is optimized, improving the efficiency of snail crushing. Simultaneously, multiple snail placement grooves are provided within the snail placement plate, effectively solving the problem of difficulty in neatly arranging snails during simultaneous crushing and microscopic examination of a large number of snails, and the technical problem of cross-contamination of fluids from adjacent crushed snails.

The invention provides a primer group, a kit and a method for detecting MNP marker sites of schistosoma and oncomelania in a water environment and application of the primer group, the kit and the method. The primer group comprises at least one of a first primer pair to a fourteenth primer pair, each primer pair comprises a forward primer and a reverse primer, and the forward primer of the first primer pair, the reverse primer of the first primer pair, the forward primer of the fourteenth primer pair and the reverse primer of the fourteenth primer pair are sequentially shown as SEQ ID NO: 1 to SEQ ID NO: 28 in a sequence table. The primer group has amplification compatibility, amplification can be carried out in a reaction system by adopting a super-multiplex PCR technology, amplification of 14 MNP marker sites in the reaction system is realized, sequence analysis of amplification products is carried out by fusing a next-generation sequencing platform, and identification of schistosoma japonicum and oncomelania hupehensis by one reaction is realized. The method has a comprehensive, efficient, sensitive and accurate detection effect.

This invention discloses a method for the simultaneous quantitative detection of *Schistosoma japonicum* and its host, *Oncomelania hupehensis*, belonging to the field of molecular biology. The primer sequences involved are as follows: *Oncomelania hupehensis* pre-primer: 5'-GCCAGTGTGAAAAGCGAAAAAT-3'; *Oncomelania hupehensis* post-primer: 5'-AAAAGGACTTTCACCTCTACCTTACG-3'; fluorescent probe: 5'-CATGCGGTTTCGGC-3'; *Schistosoma japonicum* pre-primer: 5'-GCGTTCGAACCTATGCCAAA-3'; *Schistosoma japonicum* post-primer: 5'-GAGCGGTAATCTAGAGTCACAGCTAA-3'; fluorescent probe: 5'-AGGACAACAAGCCTC-3'. This method can rapidly diagnose *Oncomelania hupehensis* and *Schistosoma japonicum* infection.

The invention discloses a method for specifically detecting poecilobdella glauca and application of the poecilobdella glauca. The primer probe group capable of specifically detecting the poecilobdella glauca is designed on the basis that the ND1 gene of the poecilobdella glauca serves as a target, the detection sensitivity reaches 2.88 * 100 copies / mu L, and meanwhile high repeatability and negative stability are achieved. The invention provides a new molecular target for detecting the poecilobdella glauca, and provides a simpler and more convenient detection means for disease detection and monitoring of the poecilobdella glauca in the aquaculture process.