9 results about "Oncomelania" patented technology

The invention discloses an ecological regulation and control model for oncomelania indoor culture, and relates to the technical field of oncomelania culture regulation and control. Comprising the following steps: establishing a matching relationship between the number of indoor breeding units and the total oncomelania breeding amount for realizing standardized control of the initial oncomelania number of each breeding unit, ensuring the initial ecological balance of an experiment system, and determining the environment regulation priority of the indoor oncomelania breeding units based on the matching relationship. According to the method, the environmental parameter weight is dynamically corrected based on the risk intensity of different propagation stages of the schistosome, and the bottleneck that a static prevention and control strategy is difficult to adapt to the life cycle change of parasites is broken through; the biological rhythm of the oncomelania snail population can be accurately responded by environment regulation and control, the sustainability of indoor culture of the oncomelania snails is guaranteed to a certain extent, and the risk of schistosomiasis transmission is reduced.

This invention relates to a zero-shot Oncomelania snail identification method and system based on a structured knowledge base and attention guidance, belonging to the field of image processing technology. The method includes: acquiring original images of Oncomelania snails; generating an attention map using a pre-trained model to locate and crop discriminative regions; constructing a structured Oncomelania snail knowledge base and guiding a large language model to generate fine-grained text descriptions; extracting image and text features separately, calculating region weights and text weights; and determining the snail species through weighted image-text similarity matching. This invention achieves zero-shot identification without annotating image data, effectively suppresses interference from complex backgrounds, significantly reduces data and annotation costs, and improves identification efficiency and robustness, making it suitable for low-cost Oncomelania snail monitoring in field environments.

This invention discloses a self-cleaning, anti-interference snail crushing device and its usage method for microscopic examination. The device includes a main body, a flat crushing unit, a snail placement unit, a pressure plate cleaning unit, and a snail placement plate detachably installed on the upper surface of the snail placement unit for placing and positioning snails. It employs automated pressure application technology, solving the technical problem that manual crushing methods inevitably cause uneven stress on the snails and varying degrees of crushing when handling a large number of snails for microscopic examination, thus affecting the quality of subsequent microscopic examinations. Furthermore, by integrating the pressure plate cleaning unit into the device, the pressure plate cleaning scheme is optimized, improving the efficiency of snail crushing. Simultaneously, multiple snail placement grooves are provided within the snail placement plate, effectively solving the problem of difficulty in neatly arranging snails during simultaneous crushing and microscopic examination of a large number of snails, and the technical problem of cross-contamination of fluids from adjacent crushed snails.

The application discloses an ecological regulation model for indoor breeding of oncomelania, and relates to the technical field of snail breeding regulation. The model comprises a matching relationship between the number of indoor breeding units and the total amount of oncomelania breeding, which is used for realizing the standardized control of the initial oncomelania quantity of each breeding unit, ensuring the initial ecological balance of the experimental system, and determining the environmental regulation priority of the indoor oncomelania breeding unit based on the matching relationship. The application dynamically corrects the weight of the environmental parameter based on the risk intensity of different transmission stages of schistosoma, breaks through the bottleneck that the static prevention and control strategy is difficult to adapt to the change of the life cycle of parasites, and further realizes real-time monitoring of the fluctuation of biological characteristic parameters in the mating period, the pregnancy period and the reproductive period, so that the environmental regulation can accurately respond to the biological rhythm of the oncomelania population, ensures the sustainability of the indoor breeding of oncomelania to a certain extent, and reduces the risk of schistosomiasis transmission.

The invention provides a primer group, a kit and a method for detecting MNP marker sites of schistosoma and oncomelania in a water environment and application of the primer group, the kit and the method. The primer group comprises at least one of a first primer pair to a fourteenth primer pair, each primer pair comprises a forward primer and a reverse primer, and the forward primer of the first primer pair, the reverse primer of the first primer pair, the forward primer of the fourteenth primer pair and the reverse primer of the fourteenth primer pair are sequentially shown as SEQ ID NO: 1 to SEQ ID NO: 28 in a sequence table. The primer group has amplification compatibility, amplification can be carried out in a reaction system by adopting a super-multiplex PCR technology, amplification of 14 MNP marker sites in the reaction system is realized, sequence analysis of amplification products is carried out by fusing a next-generation sequencing platform, and identification of schistosoma japonicum and oncomelania hupehensis by one reaction is realized. The method has a comprehensive, efficient, sensitive and accurate detection effect.

The invention discloses a detection kit for identifying oncomelania hirsuta of Yunnan province and belongs to the technical field of molecular biology. The nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO. 1, and the single nucleotide polymorphism of a basic group at the 75th site is 75A / G or 75T; and the single nucleotide polymorphism of the basic group at the 420th site is 420C / T or 420A. According to the invention, a method capable of distinguishing oncomelania snail populations in Sichuan and Yunnan is established for the first time, whether oncomelania snails are from Sichuan or Yunnan can be rapidly distinguished by utilizing the provided SNP molecular marker or through simple PCR amplification and electrophoresis detection, and scientific support is provided for frequently-communicated input (positive) oncomelania snails in two adjacent places.

This invention discloses a method for the simultaneous quantitative detection of *Schistosoma japonicum* and its host, *Oncomelania hupehensis*, belonging to the field of molecular biology. The primer sequences involved are as follows: *Oncomelania hupehensis* pre-primer: 5'-GCCAGTGTGAAAAGCGAAAAAT-3'; *Oncomelania hupehensis* post-primer: 5'-AAAAGGACTTTCACCTCTACCTTACG-3'; fluorescent probe: 5'-CATGCGGTTTCGGC-3'; *Schistosoma japonicum* pre-primer: 5'-GCGTTCGAACCTATGCCAAA-3'; *Schistosoma japonicum* post-primer: 5'-GAGCGGTAATCTAGAGTCACAGCTAA-3'; fluorescent probe: 5'-AGGACAACAAGCCTC-3'. This method can rapidly diagnose *Oncomelania hupehensis* and *Schistosoma japonicum* infection.

The present application relates to the technical field of parasite prevention and treatment, in particular to the application of a mutant strain of bacillus subtilis in killing oncomelania and a mutagenesis method. The present application adopts a mutant strain of bacillus subtilis to kill oncomelania. The mutant strain is any one or combination of ARTP-129 or ARTP-154. ARTP-129 was preserved in China Center for Type Culture Collection on January 6, 2025, with the preservation number of CCTCC M 2025036. ARTP-154 was preserved in China Center for Type Culture Collection on January 6, 2025, with the preservation number of CCTCC M 2025037. Through the above technical solution, the mutant strain of bacillus subtilis is adopted to kill oncomelania. The present application is conducive to improving the efficiency of killing oncomelania and has the advantages of green environmental protection, no harmful residues and environmental improvement.

The invention discloses a method for specifically detecting poecilobdella glauca and application of the poecilobdella glauca. The primer probe group capable of specifically detecting the poecilobdella glauca is designed on the basis that the ND1 gene of the poecilobdella glauca serves as a target, the detection sensitivity reaches 2.88 * 100 copies / mu L, and meanwhile high repeatability and negative stability are achieved. The invention provides a new molecular target for detecting the poecilobdella glauca, and provides a simpler and more convenient detection means for disease detection and monitoring of the poecilobdella glauca in the aquaculture process.