The invention provides a schistosomiasis electrochemistry sensing quick determination kit, a detection method and a preparation method of the kit. The kit comprises an electrochemistry sensing array, an enzyme conjugate operating fluid, an enzyme substrate, a negative comparison product, a positive comparison product, a sample diluent and a scrubbing solution, wherein the electrochemistry sensing array comprises a printing carbon electrode and a surface-co-assembled schistosome antigen layer and an adsorbed sealing agent layer; a schistosome antigen is the mixture of SjE16 and SEA, an enzyme conjugate working solution is a second-antibody BSA (bovine serum albumin) solution containing a horseradish peroxidase mark, and the enzyme substrate is the mixture of tetramethyl benzidine and hydrogen peroxide; and the negative comparison product is normal blood serum, the positive comparison product is schistosome infection blood serum, and the sample diluent and the scrubbing solution are phosphate buffer solutions containing surface active agents. The kit provided by the invention has the advantages that the preparation is simple, the cost is low, the multi-sample analysis is performed, detection sensitivity, specificity and reproducibility are good, and the schistosome electrochemistry sensing quick determination kit and the detection method are hopefully used for screening and monitoring schistosome patients in an affected area.