siRNA of schistosoma japonicum SjELAV-like 2 genes and application thereof

A technology for schistosomiasis and schistosomiasis, applied in the fields of molecular biology and biomedicine, can solve the problems of low meiotic efficiency of mature oocytes and other problems

Active Publication Date: 2018-11-13
SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ELAV-like protein family has four members: ELAV-like 1, ELAV-like 2, ELAV-like 3, and ELAV-like 4. Among them, ELAV-like 1 is widely expressed in various tissues, and ELAV-like 3 and ELAV-like 4 serve as Self-antigens have been found in neoplastic encephalopathy, a multisystem neurological disorder. ELAV-like 2 protein is not only expressed in the nervous system of various species, but also specifically expressed in the gonads of mice, testes, ovaries and early embryos of Xenopus laevis , and studies have shown that premature reduction of ELAV-like 2 during oocyte growth in mice leads to ineffective meiosis in mature oocytes

Method used

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  • siRNA of schistosoma japonicum SjELAV-like 2 genes and application thereof
  • siRNA of schistosoma japonicum SjELAV-like 2 genes and application thereof
  • siRNA of schistosoma japonicum SjELAV-like 2 genes and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 In vivo RNA interference screening for optimal dsRNA

[0029] 1. Method steps

[0030] 1.1 siRNA molecules and SjELAV-like 2 Preparation of Gene Real-time Quantitative PCR Primers

[0031] Schistosoma SjELAV-like 2 The gene sequence (MG515726) was analyzed and compared, and three pairs of dsRNA were designed and synthesized. The principles of siRNA design are as follows:

[0032] 1. Starting from the AUG start codon of the transcript (mRNA), look for the "AA or NA" double sequence, and write down the 19 nucleotide sequence at its 3' end as a potential siRNA target site. Both sense and antisense strands were designed using these 19 bases (excluding AA or NA repeats).

[0033] 2. Avoid selecting sequences of interest near start codons or nonsense regions.

[0034] 3. The GC content of the siRNA sequence should be around 30%-60%.

[0035] 4. When designing siRNA, do not target the 5' and 3' non-translated regions (untranslated regions, UTRs), because ...

Embodiment 2

[0058] Example 2 Long-term RNA interference in vivo

[0059] 1. Method steps

[0060] 1.1 dsRNA injection and worm collection

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Abstract

The invention discloses siRNA of schistosoma japonicum SjELAV-like 2 genes. The siRNA is selected from one pair of a combination of more than optional two pairs as follows: nucleotide sequences shownas SEQ ID NO.1 and SEQ ID NO.2, nucleotide sequences shown as SEQ ID NO.3 and SEQ ID NO.4, and nucleotide sequences shown as SEQ ID NO.5 and SEQ ID NO.6. The invention further discloses the application of the siRNA of schistosoma japonicum SjELAV-like 2 genes. The siRNA of schistosoma japonicum SjELAV-like 2 genes disclosed by the invention is capable of obviously inhibiting transcription of the schistosoma japonicum SjELAV-like 2 genes and obviously reducing the liver egg hatching rate of schistosomiasis infected mice, and is applicable to preparation of drugs for treating schistosomiasis.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and biomedicine, in particular to a Schistosoma japonicum SjELAV- like 2 Gene siRNA and its application. Background technique [0002] Schistosomiasis is caused by schistosomiasis infection, a major parasitic disease that seriously endangers human and animal health and economic development, and is still a public health problem that needs attention. At present, chemical drugs such as praziquantel are mainly used in the treatment of schistosomiasis. Although the drug has a therapeutic effect on individuals infected with schistosomiasis, drug treatment cannot solve the problem of repeated infection, nor can it achieve the purpose of prevention. Therefore, it is necessary to find new drugs for the treatment of schistosomiasis. [0003] The ELAV-like (embryonic lethal abnormal vision like) protein family is a class of mRNA-binding proteins that contain three highly conserved RNA recognit...

Claims

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Application Information

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IPC IPC(8): C12N15/113A61K31/7105A61P33/12
CPCA61P33/12A61K31/7105C12N15/113C12N2310/14Y02A50/30
Inventor 金亚美张媛媛许蓉何川川李肖纯程贵凤刘金明李浩古少鹏
Owner SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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