The invention discloses a method from extracting
amylase from liquorice and application thereof. The method comprises the following steps: a. dried liquorice is ground; b. the ground liquorice is put into an extraction tank,
petroleum ether is added for reflowing and
degreasing, and then filtered out to obtain degreased residue; c. the residue is added with
distilled water for extraction in normal termperature, repeatedly poached and extracted for three times, and triple extraction solutions are combined; d. the extraction solution is decompressed and concentrated to cause the volume of the solution to be the liquorice
powder in the step a, thus obtaining a concentrated solution; e. a concentrated solution is added into the concentrated solution in the step d, and then the concentrated
mixed solution stands overnight and precipitates; f. the concentrated
mixed solution is centrifugalized to obtain the
precipitation, dried, and then washed with absolute ethyl
alcohol,
acetone and aether after is extracted by a Sevag method; g. semi-permeable membrane with the interception molecular weight of 1000-10000 is used for dialyzing in water, the liquorice
amylase is obtained by freezing and
drying. The liquorice
amylase is applied to the preparation of
medicine treating or preventing
osteoarthritis. The liquorice amylase has definite
active ingredient, definite healing effect and no toxic
side effect and the extraction method is easy to be implemented.