Method for extracting amylase from liquorice and application thereof

Abstract

The invention discloses a method from extracting amylase from liquorice and application thereof. The method comprises the following steps: a. dried liquorice is ground; b. the ground liquorice is put into an extraction tank, petroleum ether is added for reflowing and degreasing, and then filtered out to obtain degreased residue; c. the residue is added with distilled water for extraction in normal termperature, repeatedly poached and extracted for three times, and triple extraction solutions are combined; d. the extraction solution is decompressed and concentrated to cause the volume of the solution to be the liquorice powder in the step a, thus obtaining a concentrated solution; e. a concentrated solution is added into the concentrated solution in the step d, and then the concentrated mixed solution stands overnight and precipitates; f. the concentrated mixed solution is centrifugalized to obtain the precipitation, dried, and then washed with absolute ethyl alcohol, acetone and aether after is extracted by a Sevag method; g. semi-permeable membrane with the interception molecular weight of 1000-10000 is used for dialyzing in water, the liquorice amylase is obtained by freezing and drying. The liquorice amylase is applied to the preparation of medicine treating or preventing osteoarthritis. The liquorice amylase has definite active ingredient, definite healing effect and no toxic side effect and the extraction method is easy to be implemented.

Description

technical field

[0001] The invention relates to the technical field of medicine, more specifically to a method for extracting licorice polysaccharide from licorice, and also relates to the use of the licorice polysaccharide extracted from licorice in medicines for preventing and treating osteoarthritis. Background technique

[0002] Articular cartilage tissue is mainly composed of chondrocytes and extracellular matrix (ECM), and the main components of ECM are type II collagen, proteoglycan aggregates and water. The degradation and repair of normal articular cartilage matrix are in a continuous dynamic equilibrium state, which is maintained by the secretion of cartilage matrix components by chondrocytes. Osteoarthritis is a chronic joint disease characterized by focal articular cartilage degeneration, bone loss, joint edge osteophyte formation, joint deformity and subchondral bone density (sclerosis). osteoarthritis. With the advent of an aging society and the extension of ...

Images