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Method for extracting amylase from liquorice and application thereof

A technology of licorice and polysaccharide, applied in the field of medicine, can solve the problem of no prevention or treatment of osteoarthritis, and achieve the effects of strong administration effect, long duration and small toxic and side effects

Active Publication Date: 2009-09-09
广州南峰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant report on the treatment of osteoarthritis with licorice alone, and there is no report on the prevention or treatment of osteoarthritis with polysaccharides extracted from licorice

Method used

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  • Method for extracting amylase from liquorice and application thereof
  • Method for extracting amylase from liquorice and application thereof
  • Method for extracting amylase from liquorice and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: the method for extracting licorice polysaccharide from licorice

[0043] A, the dry licorice is powdered into 80 or 85 or 90 or 95 or 100 mesh powder with pulverizer;

[0044] B. Put the powder into an extraction tank, add 3 times (w / v, g / mL) of petroleum ether (boiling range is 60°C to 90°C) and reflux for 30 or 35 or 42 or 51 or 56 or 60 minutes, degrease, Repeat this step twice, filter out petroleum ether, obtain the residue after degreasing;

[0045] C. Add 7 times (w / v, g / mL) distilled water to the residue and extract at 100°C for 1 hour, repeat this step three times, and combine the three extracts;

[0046] D, 50 ℃ decompression (1 × 10 4 pa) concentrating the extract so that the volume of the solution is 5 times the weight (g) of the licorice powder used in step A to obtain a concentrate;

[0047] E. Add 3 times the volume of 95% (v / v) ethanol to the concentrated solution in step D, and let it stand overnight so as to fully precipitate;

[0048]...

Embodiment 2

[0050] Embodiment 2: the method for extracting licorice polysaccharide from licorice

[0051] A, the dry licorice is powdered into 80 or 85 or 90 or 95 or 100 mesh powder with pulverizer;

[0052] B. Put it into an extraction tank, add 4 times (w / v, g / mL) of petroleum ether (boiling range: 60°C to 90°C) to reflux for 35 minutes to degrease, repeat this step twice, filter out the petroleum ether, and obtain the degreased the residue of

[0053] C. Add 8 times (w / v, g / mL) distilled water to the residue and extract at 100°C for 1.5 hours, repeat this step three times, and combine the three extracts;

[0054] D, 55 ℃ decompression (2 × 10 4 pa) concentrating the extract so that the volume of the solution is 6 times the weight (g) of the licorice powder used in step A to obtain a concentrate;

[0055] E. Add 3 times the volume of 95% (v / v) ethanol to the concentrated solution in step D, and let it stand overnight so as to fully precipitate;

[0056] F, centrifuge to obtain prec...

Embodiment 3

[0058] Embodiment 3: the method for extracting licorice polysaccharide from licorice

[0059] A, the dry licorice is powdered into 80 or 85 or 90 or 95 or 100 mesh powder with pulverizer;

[0060] B. Put it into an extraction tank, add 5 times (w / v, g / mL) of petroleum ether (boiling range: 60°C to 90°C) and reflux for 40 minutes to degrease, repeat this step twice, filter out the petroleum ether, and obtain the degreased the residue of

[0061] C. Add 8 times (w / v, g / mL) distilled water to the residue to extract at 100°C for 2 hours, repeat this step three times, and combine the three extracts;

[0062] D, 60 ℃ decompression (3 × 10 4 pa) concentrating the extract so that the solution volume (mL) is 7 times the weight (g) of the licorice powder used in step A to obtain a concentrate;

[0063] E. Add 3 times the volume of 95% (v / v) ethanol to the concentrated solution in step D, and let it stand overnight so as to fully precipitate;

[0064] F, centrifuge to obtain precipit...

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Abstract

The invention discloses a method from extracting amylase from liquorice and application thereof. The method comprises the following steps: a. dried liquorice is ground; b. the ground liquorice is put into an extraction tank, petroleum ether is added for reflowing and degreasing, and then filtered out to obtain degreased residue; c. the residue is added with distilled water for extraction in normal termperature, repeatedly poached and extracted for three times, and triple extraction solutions are combined; d. the extraction solution is decompressed and concentrated to cause the volume of the solution to be the liquorice powder in the step a, thus obtaining a concentrated solution; e. a concentrated solution is added into the concentrated solution in the step d, and then the concentrated mixed solution stands overnight and precipitates; f. the concentrated mixed solution is centrifugalized to obtain the precipitation, dried, and then washed with absolute ethyl alcohol, acetone and aether after is extracted by a Sevag method; g. semi-permeable membrane with the interception molecular weight of 1000-10000 is used for dialyzing in water, the liquorice amylase is obtained by freezing and drying. The liquorice amylase is applied to the preparation of medicine treating or preventing osteoarthritis. The liquorice amylase has definite active ingredient, definite healing effect and no toxic side effect and the extraction method is easy to be implemented.

Description

technical field [0001] The invention relates to the technical field of medicine, more specifically to a method for extracting licorice polysaccharide from licorice, and also relates to the use of the licorice polysaccharide extracted from licorice in medicines for preventing and treating osteoarthritis. Background technique [0002] Articular cartilage tissue is mainly composed of chondrocytes and extracellular matrix (ECM), and the main components of ECM are type II collagen, proteoglycan aggregates and water. The degradation and repair of normal articular cartilage matrix are in a continuous dynamic equilibrium state, which is maintained by the secretion of cartilage matrix components by chondrocytes. Osteoarthritis is a chronic joint disease characterized by focal articular cartilage degeneration, bone loss, joint edge osteophyte formation, joint deformity and subchondral bone density (sclerosis). osteoarthritis. With the advent of an aging society and the extension of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P19/02A61K36/484
Inventor 汪晖陈廖斌李小军汪巍何文博张逸杰范雯
Owner 广州南峰生物科技有限公司
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