2results about How to "Lower hatch rate" patented technology

The application discloses CTL-S1 A gene and an application of miR-let-7 in regulation of reproductive development of drosophila bacterea CTL-S1 The gene is specifically highly expressed in ovaries of drosophila bacterea, and the expression of the gene is inhibited by RNA interference CTL-S1 The expression of the gene can reduce the hatching rate of offspring. Through analysis of an RNA immunoprecipitation double luciferase reporter system, it is found that miR-let-7 and CTL-S1 there is a target relationship. Overexpression of miR-let-7 can cause CTL-S1 the expression amount of the gene to be significantly reduced, and a phenotype similar to the expression of the gene being inhibited by RNA interference CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S is generated, and the hatching rate of offspring is reduced. The application provides a potential gene target for developing a genetic control strategy for destroying the reproduction of drosophila bacterea, provides a new idea for subsequent biological control of insects by using the sterile technique, and has a wide application prospect.

The application discloses a low-temperature tolerance related GLUR6 gene of Pomacea canaliculata, an encoding protein and application thereof, and belongs to the technical field of biotechnology.The nucleotide sequence of the GLUR6 gene is shown as SEQ ID NO:1; the GLUR6 gene can be applied as a target for pesticide screening and biological control of Pomacea canaliculata. The application further provides dsRNA of the GLUR6 gene. The green and environment-friendly biological pesticide developed according to the dsRNA sequence can reduce the survival rate of Pomacea canaliculata in a low-temperature environment and the hatching rate of Pomacea canaliculata eggs. The application is fast, effective and highly reproducible, and is an important supplement to the GLUR6 gene family. The application reduces the low-temperature tolerance of Pomacea canaliculata by blocking the expression of the GLUR6 gene in Pomacea canaliculata, so as to achieve the purpose of controlling the overwintering of Pomacea canaliculata in winter soil, reducing the reproduction of Pomacea canaliculata and continuing the northward diffusion of Pomacea canaliculata. The application has important significance for the research and determination of the biology, ecology and new insecticides of Pomacea canaliculata and the mechanism research of the low-temperature tolerance of Pomacea canaliculata.