5results about How to "Lower hatch rate" patented technology

The application discloses CTL-S1 A gene and an application of miR-let-7 in regulation of reproductive development of drosophila bacterea CTL-S1 The gene is specifically highly expressed in ovaries of drosophila bacterea, and the expression of the gene is inhibited by RNA interference CTL-S1 The expression of the gene can reduce the hatching rate of offspring. Through analysis of an RNA immunoprecipitation double luciferase reporter system, it is found that miR-let-7 and CTL-S1 there is a target relationship. Overexpression of miR-let-7 can cause CTL-S1 the expression amount of the gene to be significantly reduced, and a phenotype similar to the expression of the gene being inhibited by RNA interference CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S is generated, and the hatching rate of offspring is reduced. The application provides a potential gene target for developing a genetic control strategy for destroying the reproduction of drosophila bacterea, provides a new idea for subsequent biological control of insects by using the sterile technique, and has a wide application prospect.

The present invention provides an application of pulegone in pest control, and relates to the technical field of pest control. The pulegone can effectively kill adult golden apple snails at a high concentration, and can inhibit the laying of eggs of golden apple snails and reduce the hatching rate of eggs laid by golden apple snails at a low concentration. At the same time, spraying golden apple snail egg masses at a low concentration can effectively inhibit the hatching of eggs and reduce the hatching rate. That is, the use of pulegone, a plant-derived component, can kill golden apple snails, and can also effectively destroy the reproductive reproduction of golden apple snails, further realizing the prevention and control of golden apple snails, and on this basis, pulegone can also kill harmful snails such as striped snails.

The invention relates to the technical field of pharmaceutical biology, in particular to application of (-)-epicatechin to inhibition of toxicity caused by MEZ. The toxicity caused by the MEZ is developmental toxicity or hepatotoxicity. The developmental toxicity comprises the following symptoms: embryo malformation, embryo apoptosis and developmental morphology abnormality. The hepatotoxicity comprises liver development delaying, cholesterol and lipid metabolism disorder, liver injury and liver cell apoptosis. According to the research, the effect of the L-epicatechin on inhibiting MEZ-induced developmental toxicity and hepatotoxicity is found, embryo injury and liver injury caused by MEZ can be effectively relieved, and the pathogenesis of the MEZ-induced developmental toxicity and hepatotoxicity can be further understood.

The application discloses a low-temperature tolerance related GLUR6 gene of Pomacea canaliculata, an encoding protein and application thereof, and belongs to the technical field of biotechnology.The nucleotide sequence of the GLUR6 gene is shown as SEQ ID NO:1; the GLUR6 gene can be applied as a target for pesticide screening and biological control of Pomacea canaliculata. The application further provides dsRNA of the GLUR6 gene. The green and environment-friendly biological pesticide developed according to the dsRNA sequence can reduce the survival rate of Pomacea canaliculata in a low-temperature environment and the hatching rate of Pomacea canaliculata eggs. The application is fast, effective and highly reproducible, and is an important supplement to the GLUR6 gene family. The application reduces the low-temperature tolerance of Pomacea canaliculata by blocking the expression of the GLUR6 gene in Pomacea canaliculata, so as to achieve the purpose of controlling the overwintering of Pomacea canaliculata in winter soil, reducing the reproduction of Pomacea canaliculata and continuing the northward diffusion of Pomacea canaliculata. The application has important significance for the research and determination of the biology, ecology and new insecticides of Pomacea canaliculata and the mechanism research of the low-temperature tolerance of Pomacea canaliculata.

The invention relates to the technical field of pesticides, and discloses an insecticidal composition and application thereof.The insecticidal composition comprises active ingredients and is characterized in that the active ingredients comprise hypericin and calcium cyanamide, and the mass ratio of the hypericin to the calcium cyanamide is 1: (1000-10000). The invention also discloses an application of the insecticidal composition in prevention and control of mosquito insects. The active ingredients in the insecticidal composition are used in a proper proportion range, the fatality rate of the insecticidal composition on mosquito insects is remarkably higher than that of each single agent, the insecticidal composition has a synergistic effect in a certain proportion range, the control effect on the mosquito insects is remarkably improved, and the insecticidal composition is low in application dosage, low in toxicity and small in harm to the environment and other animals and has a good application prospect. The method is of great significance in preventing transmission of dengue viruses and other mosquito-borne viruses, protecting the environment and reducing harm to the environment.