P. canaliculata low temperature tolerance related glur6 gene, encoding protein and application thereof

Abstract

The application discloses a low-temperature tolerance related GLUR6 gene of Pomacea canaliculata, an encoding protein and application thereof, and belongs to the technical field of biotechnology.The nucleotide sequence of the GLUR6 gene is shown as SEQ ID NO:1; the GLUR6 gene can be applied as a target for pesticide screening and biological control of Pomacea canaliculata. The application further provides dsRNA of the GLUR6 gene. The green and environment-friendly biological pesticide developed according to the dsRNA sequence can reduce the survival rate of Pomacea canaliculata in a low-temperature environment and the hatching rate of Pomacea canaliculata eggs. The application is fast, effective and highly reproducible, and is an important supplement to the GLUR6 gene family. The application reduces the low-temperature tolerance of Pomacea canaliculata by blocking the expression of the GLUR6 gene in Pomacea canaliculata, so as to achieve the purpose of controlling the overwintering of Pomacea canaliculata in winter soil, reducing the reproduction of Pomacea canaliculata and continuing the northward diffusion of Pomacea canaliculata. The application has important significance for the research and determination of the biology, ecology and new insecticides of Pomacea canaliculata and the mechanism research of the low-temperature tolerance of Pomacea canaliculata.

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