8 results about "Xenopus" patented technology

1. Name of the designed product: doll (Rana taiwaniana). 2. Use of the designed product: for dolls. 3. Design points of the designed product: in shape. 4. Picture or photograph best indicating the design points: perspective view.

The invention discloses a construction method and an application of Elizabeth mileli for expressing Nanoluc luciferase. The Elizabeth mileli can be used for expressing Nanoluc luciferase. The coding gene of the Nanoluc luciferase is knocked into the genome of the Elizabeth mileli FL160902 strain in a fixed-point manner through a homologous recombination technology, so that the report strain capable of stably expressing the luciferase is successfully constructed. The reporter strain has normal growth characteristics, and the in-vitro detection sensitivity can reach 100 CFU. By utilizing a xenopus laevis infection model, the real-time dynamic tracing of the report strain in a living animal body is realized for the first time. The Nluc report strain constructed by the invention has the characteristics of strong luminescence stability, high detection sensitivity and the like, and provides a high-sensitivity visual research tool for analyzing the infection dynamic state and pathogenic mechanism of Elizabeth mileli.

The invention provides a method for identifying limb regeneration heterogeneity between salamander and xenopus laevis, and relates to the technical field of biology. Comprising the following steps: respectively extracting samples of salamander and xenopus at different stages, and carrying out RNA extraction and high-throughput sequencing on the samples to obtain original data; evaluating the quality of the original data, performing quality control on the original data, and removing low-quality basic groups or linker pollution sequences; constructing a salamander reference genome index, comparing the quality-controlled data with a reference genome, and outputting comparison data; outputting an original count matrix from the comparison data, and calculating a TPM (Trusted Platform Module) value by using the original count matrix in an R language environment; using sequence alignment to extract common genes of the salamander and the xenopus; and performing principal component analysis and correlation analysis on the acquired data. Through transcriptome analysis and sequencing, the molecular mechanism of the limb regeneration capacity heterogeneity of the salamander and the xenopus laevis is analyzed, and guidance is provided for research of other regeneration capacities.

This invention discloses a transgenic recombinant immune cell that specifically targets tumors. The recombinant immune cell comprises a chimeric polypeptide and a chimeric antigen receptor; the chimeric polypeptide includes a first extracellular region, a first transmembrane region, and a first intracellular region with HLA-G protein binding activity, wherein the N-terminus of the first transmembrane region is connected to the C-terminus of the first extracellular region, and the N-terminus of the first intracellular region is connected to the C-terminus of the first transmembrane region; the first transmembrane region includes the transmembrane region of the Notch receptor protein from Xenopus laevis; the antigen-chimeric receptor does not have HLA-G protein binding activity. This recombinant immune cell significantly improves the precise recognition of tumor cells by immune cells, reduces off-target effects of cell therapy, and accurately and effectively distinguishes tumor cells from normal cells, providing an effective target and targeting method for the treatment of broad-spectrum tumors (especially solid tumors).

This invention relates to the field of cell biology, and more particularly to a method for constructing an artificial cell model in vitro based on extracts from Xenopus laevis egg cells. The method includes: mixing cytoplasmic extracts from Xenopus laevis egg cells, demembranous sperm, and tubulin, dispersing the mixture in an oil phase to obtain a reconstructed system; reacting the reconstructed system at 20-24°C for at least 30 minutes, resulting in the formation of a phospholipid bilayer-based cytoplasmic membrane structure around the cell. This invention provides a method for constructing artificial cells by selecting cytoplasmic extracts from Xenopus laevis egg cells, adding specific substances, and reacting under specific conditions to allow the extracts to self-assemble into cell structures in vitro, simultaneously forming a cytoplasmic membrane. The method provided by this invention offers important clues to the origin and evolution of cells, and the resulting artificial cell structures are highly similar to normal cells, possessing significant application value.

A method of promoting increased cellular hydration in a multicellular organism that is capable of intracellular water permeation includes the step of causing the multicellular organism to ingest an aqueous solution that contains an amount of a carbohydrate clathrate component. There is also a step of enhancing the intracellular permeation. The multicellular organism contains aquaporins, and the causing step involves interaction of the composition with the aquaporins. The cellular hydration promoted and caused by the method is corroborated by a test that uses human-aquaporin-expressed frog oocytes. The test uses single cell Xenopus laevis frog oocytes having expressed human aquaporin AGP1 water channels. There is also a beverage composition that increases lifespan in the multicellular organism, and a beverage composition that promotes cellular hydration when ingested by a multicellular organism.

The present application relates to an antibacterial product based on olive leaf extract, belonging to the technical field of antibacterial care. The product is based on olive leaf extract rich in polyphenol active ingredients, combined with bacteriostatic polypeptides derived from Xenopus laevis as the core active component, to achieve synergistic antibacterial effect. Studies have shown that the combination has rapid and strong inhibitory and killing ability on common pathogenic microorganisms such as Staphylococcus aureus and Candida albicans, and is significantly better than single component. In vitro test results show that the product can achieve strong bacteriostatic and bactericidal effect in a short time, and has certain sustained bacteriostatic ability. Compared with traditional antibiotics or single plant extract, the present application improves the antibacterial efficiency and reduces the risk of drug resistance through the synergistic mechanism of "natural plant matrix + functional polypeptide", and has good safety and microecological friendliness, and is suitable for daily care and infection protection of female reproductive system.

This invention discloses a Torreya grandis MATE transporter protein TgMATE72, its encoding gene, and its applications. The amino acid sequence of the transporter protein TgMATE72 is shown in SEQ ID NO.2, and the nucleotide sequence of the encoding gene is shown in SEQ ID NO.1. This invention also provides the application of the transporter protein TgMATE72 and its encoding gene in regulating the transport of proanthocyanidin precursors and increasing proanthocyanidin content in plants. Based on the combined analysis of transcriptome data from Torreya grandis seed development and the content of proanthocyanidins and their monomers, this invention screened and obtained TgMATE72, and its expression level is positively correlated with the accumulation of catechin monomers. Sequence alignment and phylogenetic analysis show that the transporter protein TgMATE72 belongs to the plant MATE transporter protein family. Heterologous expression and transport activity detection results in Xenopus laevis oocytes show that TgMATE72 can mediate the transport of catechin monomers. This invention provides gene resources and application methods for improving proanthocyanidin-related traits in plants, and can be used for molecular breeding and quality improvement of Torreya grandis and other plants.