4 results about "Xenopus" patented technology

This invention discloses a transgenic recombinant immune cell that specifically targets tumors. The recombinant immune cell comprises a chimeric polypeptide and a chimeric antigen receptor; the chimeric polypeptide includes a first extracellular region, a first transmembrane region, and a first intracellular region with HLA-G protein binding activity, wherein the N-terminus of the first transmembrane region is connected to the C-terminus of the first extracellular region, and the N-terminus of the first intracellular region is connected to the C-terminus of the first transmembrane region; the first transmembrane region includes the transmembrane region of the Notch receptor protein from Xenopus laevis; the antigen-chimeric receptor does not have HLA-G protein binding activity. This recombinant immune cell significantly improves the precise recognition of tumor cells by immune cells, reduces off-target effects of cell therapy, and accurately and effectively distinguishes tumor cells from normal cells, providing an effective target and targeting method for the treatment of broad-spectrum tumors (especially solid tumors).

This invention relates to the field of cell biology, and more particularly to a method for constructing an artificial cell model in vitro based on extracts from Xenopus laevis egg cells. The method includes: mixing cytoplasmic extracts from Xenopus laevis egg cells, demembranous sperm, and tubulin, dispersing the mixture in an oil phase to obtain a reconstructed system; reacting the reconstructed system at 20-24°C for at least 30 minutes, resulting in the formation of a phospholipid bilayer-based cytoplasmic membrane structure around the cell. This invention provides a method for constructing artificial cells by selecting cytoplasmic extracts from Xenopus laevis egg cells, adding specific substances, and reacting under specific conditions to allow the extracts to self-assemble into cell structures in vitro, simultaneously forming a cytoplasmic membrane. The method provided by this invention offers important clues to the origin and evolution of cells, and the resulting artificial cell structures are highly similar to normal cells, possessing significant application value.

The present application relates to an antibacterial product based on olive leaf extract, belonging to the technical field of antibacterial care. The product is based on olive leaf extract rich in polyphenol active ingredients, combined with bacteriostatic polypeptides derived from Xenopus laevis as the core active component, to achieve synergistic antibacterial effect. Studies have shown that the combination has rapid and strong inhibitory and killing ability on common pathogenic microorganisms such as Staphylococcus aureus and Candida albicans, and is significantly better than single component. In vitro test results show that the product can achieve strong bacteriostatic and bactericidal effect in a short time, and has certain sustained bacteriostatic ability. Compared with traditional antibiotics or single plant extract, the present application improves the antibacterial efficiency and reduces the risk of drug resistance through the synergistic mechanism of "natural plant matrix + functional polypeptide", and has good safety and microecological friendliness, and is suitable for daily care and infection protection of female reproductive system.

This invention discloses a Torreya grandis MATE transporter protein TgMATE72, its encoding gene, and its applications. The amino acid sequence of the transporter protein TgMATE72 is shown in SEQ ID NO.2, and the nucleotide sequence of the encoding gene is shown in SEQ ID NO.1. This invention also provides the application of the transporter protein TgMATE72 and its encoding gene in regulating the transport of proanthocyanidin precursors and increasing proanthocyanidin content in plants. Based on the combined analysis of transcriptome data from Torreya grandis seed development and the content of proanthocyanidins and their monomers, this invention screened and obtained TgMATE72, and its expression level is positively correlated with the accumulation of catechin monomers. Sequence alignment and phylogenetic analysis show that the transporter protein TgMATE72 belongs to the plant MATE transporter protein family. Heterologous expression and transport activity detection results in Xenopus laevis oocytes show that TgMATE72 can mediate the transport of catechin monomers. This invention provides gene resources and application methods for improving proanthocyanidin-related traits in plants, and can be used for molecular breeding and quality improvement of Torreya grandis and other plants.