59 results about "Fat body" patented technology

Disclosed is a method of increasing lean body mass and reducing fat body mass in infants, said method comprising administration to an infant, term or preterm, a nutritional formula comprising a source of DHA and ARA. It has been found that the administration of DHA and ARA, or a source thereof, in infants can increase lean body mass and reduce fat body mass, when compared to an unsupplemented control formula, without impacting the total overall growth of the infant. This method is especially useful in preterm infants.

The invention discloses a compound feed for wild carps. Breeding experiments indicate that the compound feed for wild carps solves the problem that the artificially cultured wild carps are disadvantageous to natural wild products in terms of color, flavor and figure, namely the artificially cultured wild carps have dark body color, fat body figure and unnatural flavor, and that the compound feed for wild carps can retain the body figure and meat quality (property) of natural wild carps and promote the growth of the carps.

The invention provides five-wheat seedlings series products and a production method thereof. The five-wheat seedlings series products comprise barley grass powder, wheat seedling powder, oat seedling powder, tartary buckwheat seeding powder and highland barley wheat seeding powder. The nutrients of the five wheat seedling powder are integrated; food prepared from the five-wheat seedlings series products has the functions of conditioning hyperlipidemia, hyperglycemia and gastrointestinal peristalsis, reduces cholesterol in angiocarpy and liver, has an inhibiting effect on secondary hyperlipidemia, removes vivotoxin, recuperates blood acid-base balance, softens blood vessels, prevents cardiovascular and cerebrovascular diseases caused by hyperlipidemia, clears toxic metal elements such as lead and mercury inside a body, and has beneficial effects on increase of the effect of a blood capillary, promotion of blood circulation, reduction of hypertension, enhancement of the function of the heart, improvement of constipation, regulation of a fat body, and improvement of the body immune function.

The invention discloses expanded feed for resisting the fatty liver of tilapia and a preparation method of the expanded feed. The feed is prepared from, by mass, 3% to 8% of domestic fish meal, 15% to 25% of bean pulp, 15% to 30% of double-low rapeseed meal, 10% to 15% of cottonseed meal, 15% to 23% of strong flour, 8% to 15% of corn, 6% to 12% of DDGS, 1% to 2% of freshwater fish oil, 1% to 3% of soybean oil, 1% to 2% of monocalcium phosphate, 1% to 3% of bentonite, 1% to 2% of premix of vitamin and mineral salt, 0.1% to 0.5% of crystalline lysine, 0.1% to 0.5% of free methionine and 1% to 2% of compound fatty liver resisting preparation. Through reasonable component matching, all the nutritional requirements of tilapia are met, the stability of the feed in water is good, the tilapia grows fast, the feed conversion rate is high, and the survival rate is high. Meanwhile, according to the feed, the secretion and metabolic capability of the liver cells of the tilapia can be obviously improved, the liver function and disease resistance of the tilapia can be improved, the organ-body ratio and the fat-body ratio can be decreased, the meat taking rate can be increased, and culture benefits are good.

The invention discloses a White Spot Syndrome Virus(WSSV) resisting engineering protein VP28-CD, the preparation and application thereof. The engineering protein VP28-CD is an isolated protein with sequence of ribonucleotide of SEQ ID NO:1 plus the amino acid sequence of SEQ ID NO:2; the bacterial strain is recombinant gene engineering scherichia coli BL21 (pET-32a-VP28-CD), CCTCC No:M208032; White Spot Syndrome Virus(WSSV) is isolated from prawn and gene VP28 is obtained through PCR; silkworm is induced with colon bacillus JM109 to obtain the total mRNA of fat body of the silkworm and the total cDNA is obtained through RT-PCR; mature gene of silkworm antibacterial peptide Cecropin D is obtained through PCR, with the upper reach inserted with aspartic acid-proline at the acid hydrolysis locus; after that recombinant plasmid Pet-32a(+)-VP28-CD is constructed; the recombinant plasmid Pet-32a(+)-VP28-CD is transformed into colon bacillus BL21(DE3); under IPTG induction, the fused protein VP28-CD achieves soluable and high-level expression. The fused protein is applicable in the preparation of the drugs for curing and preventing White Spot Syndrome Virus(WSSV).

The invention discloses an anti-prawn white spot syndrome virus (WSSV) recombinant Salmonella typhimurium strain, and a preparation method and an application thereof. The preparation method comprises the steps of: extracting WSSV genomic DNA from an infected prawn, and obtaining WSSV envelope protein VP28 genes by a PCR method; extracting total RNA from a silkworm fat body, and obtaining silkworm antimicrobial peptide CD genes by a reverse transcription PCR method; constructing an eukaryotic expression plasmid pcDNA3.1(-)-VP28-CD containing the WSSV envelope protein VP28 genes and the silkworm antimicrobial peptide CD genes; and transforming into attenuated Salmonella typhimurium W0420 strain competent cells. The WSSV recombinant Salmonella typhimurium strain can be used for preparing a vaccine, which can be directly orally fed prawns. Compared with the current commonly used protein vaccines and injectable DNA vaccines, the inventive vaccine has double functions of inhibiting WSSV infection and enhancing prawn immunity.

The invention relates to a traditional Chinese medicine composition of health care weight-losing tea, which comprises the following raw material medicines by weight: 1-100 grams of rose, 1-100 grams of arabian jasmine flower, 1-100 grams of ligusticum wallichii, 1-100 grams of lotus leaf, 1-100 grams of salvia miltiorrhiza, 1-100 grams of born hawthorn, 1-100 grams of parched semen cassiae and 1-100 grams of astragalus mongholicus. The weight-losing tea has the health care effects of fatting, dredging channels, eliminating complications caused by obesity and metabolic disturbance caused by obesity and is mainly used for simple fat body types. The weight-losing tea has the beneficial effects that the formula is simple, the preparation is easy, no toxic or side effect exist when the weight-losing tea is taken for a long time, and the weight-losing effects on simple obesity are good.

The invention discloses a method for producing recombinant scorpion toxin protein by adopting a silkworm as a parasitifer, which mainly comprises the following steps: adopting a scorpion cDNA as a template, adopting 5'-ACGTAGATCTATGGATGGATATATAAG-3' containing BglII enzyme cutting site as a forward primer and adopting 5'-ACGTAGATCT TTACTTTTTTCCAC-3' containing the BglII enzyme cutting site as a reverse primer to carry out PCR gene amplification reaction in 32 cycles to obtain a scorpion toxin gene; digesting pCR2.1-scorpion toxin by restriction endonuclease BglII to obtain a scorpion toxin gene fragment; cloning the scorpion toxin gene fragment in a baculovirus transfer vector pAcGP67b to obtain a recombinant pAcGP67b-scorpion toxin gene, carrying out homologous recombination with baculovirus BmNPV DNA of a silkworm in an insect cell by cotransfection to produce a recombinant virus; expressing in a silkworm body and purifying the recombinant scorpion toxin. Most of the recombinant scorpion toxin protein expressed by a silkworm larva is retained in a fat body of the silkworm; the scorpion toxin is purified from the fat body by an affinity chromatography; and the recombinant scorpion toxin has obvious biological toxicity, is suitable for the requirement of a biopharmaceutical industrialization level and has wide application prospect in the field of future medical and clinic treatment.

The invention relates to a method for using an extrinsic green luminescence protein to label fat body stem cell. The method uses adenovirus vectors infection extrinsic green luminescence protein to get into the fat body stem cell of small mouse to displace original green luminescence protein GM small mouse fat body stem cell to label. It manifests that the Balb / c small mouse fat body stem cell of adenovirus vectors infection extrinsic green luminescence protein and the original green luminescence protein GM small mouse fat body stem cell have the same growth, value adding and diversity from morphology, mRNA and protein expressing level.

This invention publicized one kind to originate from the insect fat body organization, and to the rod-shaped viral high sensitive cell line, this invention has also publicized this cell line establishment method, as well as this cell line the use which grew into the rod-shaped viral scale metaplasia. This cell line may use for to duplicate this kind of virus, uses in the rod-shaped viral pesticide the formalization production, as well as the construction rod-shaped virus the expression vector system, the expression has commercial or the scientific value protein. Therefore it has the extremely high trade and the scientific research value.

There are provided trehalose transporter gene and a method of introducing trehalose into cells by using the gene. Candidates for the trehalose transporter genes were searched in P. vanderplanki EST, resulting in being obtained cDNA designated as Tret1. Tret1 encodes a 504 amino acid protein with 12 trans-membrane structures. Tret1 expression was induced by desiccation stress and predominant in the fat body. Functional expression of TRET1 in Xenopus oocytes showed that transport activity was specific for trehalose and independent of extracellular pH and electrochemical membrane potential. The direction of transport of TRET1 was reversible depending on the concentration gradient of trehalose. Apparent Km and Vmax of TRET1 for trehalose were extraordinarily high values. These results indicate that TRET1 is a facilitated, high-capacity trehalose-specific transporter. Tret1 is widespread in insects. Furthermore, TRET1 conferred trehalose permeability upon cells including those of vertebrates as well as insects.

A preparation method of eel feed. The feed comprises: porcine liver powder, chicken intestine powder, mixed fish meal, shrimp powder, bone meal, animal protein powder, vegetable protein powder, red worm powder, safflower powder, and chicken gizzard membrane powder, wherein the percentage is weight percentage; the formula has good effect on feeding eels, and is easy to digest; the fed eel has a high growth speed, a big size, a fat body, and low sickness rate; the feed is quite applicable to feed in various environment, and effectively overcomes the defects of slow eel growth and development, small sizes, low weight, less active values, poor palatability, and susceptibility to various infectious diseases caused by insufficiency of various nutrition of the mixed feed.

The invention relates to hawthorn lotus leaf jelly and a production method thereof, and the hawthorn lotus leaf jelly is produced by taking health hawthorn, lotus leaves and jelly powder as raw materials. In the invention, herb tea and jelly are combined, and the characteristics that the hawthorn and the lotus leaves have the functions of expanding blood vessel, reducing blood pressure and blood fat, and losing weight are utilized, so that the health-care aim can be achieved when people eat the jelly, therefore, the function unicity of the jelly can be overcome, and the jelly is particularly suitable for people with high blood pressure, hyperlipaemia, coronary heart disease and fat bodies.

The invention discloses a method for using silkworm cultured cell to express antibacterial peptide Cecropin B. The method comprises the following steps: (1) using the total RNA extracted from the fat body cells of wild silkworm chrysalis as template, adopting RT-PCR amplification to obtain wild silkworm antibacterial peptide Cecropin B gene; using 1% agarose gel electrophoresis to perform PCR product analysis to the antibacterial peptide Cecropin B gene, using a PCR purification kit of Qiagene for purification, then cloning the purified PCR product to TA vector pCR2.1 to obtain pCR2.1-Cecropin B, utilizing the dideoxynucleotide chain termination to confirm the correctness of cloned gene order; using restriction endonuclease BamHI and HindIII to digest pCR2.1-Cecropin B and obtain Cecropin B genetic fragments, then cloning rhabdovirus transfer vector pBlueBacHisa in the genetic fragments to obtain reconstituted transfer vector; performing cotransfection of the reconstituted transfer vector and silkworm wild-type nuclear polyhedrosis virus BmNPV DNA in silkworm cultured cell, performing homologous recombination to generate recombinant virus; and (3) inoculating the recombinant virus containing wild silkworm antibacterial peptide Cecropin B gene in the silkworm cultured cell, infecting at 27 DEG C for three days to express the infection, and centrifuging to collect silkworm cultured cell.

The invention relates to a traditional Chinese medicinal composition of lipid-lowering tea and an application of the traditional Chinese medicinal composition. The traditional Chinese medicinal composition is prepared from the following active ingredients: raw hawthorn fruits, roasted cassia seeds, apocynum venetum and liquorice roots. The invention further provides the application of the traditional Chinese medicinal composition. The traditional Chinese medicinal composition has the advantages that through the mixed use of the active ingredients, the efficacies of tonifying spleen, removing dampness and clearing away turbidness, calming liver and suppressing liver-yang, as well as activating and harmonizing Qi and blood are achieved; the traditional Chinese medicinal composition has the functions of promoting blood circulation and resolving dampness, as well as calming the liver and reducing turbidity, and has the main function of treating hyperlipemia, wherein the symptoms of hyperlipemia are paunch, fat body, dizziness and fullness in head, sweating when active, sticky and dirty mouth, ungratifying defecation, dark urine, purplish tongue and thick coating, and thready and slippery pulse, and the patients have the syndrome of damp obstruction and liver-yang hyperactivity.

The invention provides a manufacturing method of men's trousers and the men's trousers. The method includes the following steps that S10, the hip circumference, the thigh base circumference and the hip thickness of the human body are measured, the hip circumference H of men's suit pants is the sum of the hip circumference of the human body and the ease allowance, and the ease allowance is within the range of 6 cm to 10 cm; S20, the half thigh is determined according to the hip circumference and the thigh base circumference of the human body; S30, the small crotch width X is determined as the formula (the formula is defined in the specification) according to the half thigh and the hip thickness of the human body, if X is smaller than or equal to 5 cm, X is selected as the actual small crotch width, and if X is larger than 5 cm, 5cm-(X-5cm) is selected as the actual small crotch width. According to the technical scheme, the problems that in the prior art, fat body men's trousers are loose and large in appearance, the crotch bottom is not fit enough, excessive friction is generated in the exercise process, and wear is uncomfortable are effectively solved.

The invention discloses a diet teat and a preparation method thereof. The formula of the diet tea is as follows: 50-100 parts of homalomena occulta, 10-50 parts of ephedra, 30-80 parts of rhizoma cimicifugae, 30-60 parts of radix achyranthis bidentatae, 30-60 parts of astragalic radix, 30-50 parts of bighead atractylodes rhizome, 40-80 parts of herba epimedii, 50-200 parts of gypsum, 50-150 parts of fructus amomi, 50-100 parts of poria, 50-100 parts of rhizoma alismatis, and 50-100 parts of grifola. The preparation method comprises the following steps of 1) drying the homalomena occulta, the astragalic radix and the berba epimedii and then smashing so as to obtain powder A; 2) decocting the rest material with water for three times, wherein the water weight is 3-8 times of the rest material each time, filtering the medicine liquor after brewing, finally mixing the medicine liquor and concentrating to 0.3-0.7 times weight of the medicine liquid so as to obtain concentrated liquor B; and 3) spraying concentrated liquor B into powder A, drying to obtain the diet tea with 5% of moisture content with hot air at 100-140 DEG C, sterilizing and packaging. The diet tea can control the human body middle qi to go up and down and develop human body yang spirit, dispel false taste and enhance the harmonic taste, and has effect on regulating fat body or skinny body and losing weight.

This invention relates to novel amino substituted benzonitrile derivatives and to pharmaceutical compositions containing the novel amino substituted benzonitrile derivatives. This invention also relates to methods of treatment using amino substituted benzonitrile derivatives to prevent and / or restore age-related decline in muscle mass and strength, treat a wasting disease, treat a condition that prevents with low bone mass, increase muscle mass, increase lean body mass, decrease fat body mass, and treat bone fracture and muscle damage in mammals, including humans.

The invention discloses a compound feed capable of improving body shape of grass carps and crucian carps and a preparation method thereof, and a mixed feed prepared by adopting the compound feed capable of improving body shape of the grass carps and the crucian carps. The compound feed capable of improving body shape of the grass carps and the crucian carps comprises the following raw materials inpercentage by weight: 2-8% of imported fish meal, 15-30% of soybean meal, 15-30% of rapeseed meal, 5-10% of cottonseed meal, 8-15% of middlings, 10-20% of wheat, 5-15% of rice bran, 1-3% of soybean oil, and 1-5% of a compound premix. The compound feed is capable of solving the problems of the grass carps and the crucian carps, namely short and fat body, high aquaculture feed cost, and low aquaculture benefits.

The invention discloses a method for preparing a citrus insecticide. The citrus insecticide comprises, by weight, 5-10 parts of avermectin, 40-60 parts of a fermentation solution, 3-8 parts of a surfactant, 2-10 parts of an activator and 0-6 parts of soda ash. The citrus insecticide prepared in the invention breaks a limitation that insecticides mainly enter leaves through pores in leaf surfaces,small hydrophilic pores of the stratum corneum of the leaf surfaces and extracellular filaments of leaf cells, so the utilization rate of the citrus insecticide is greatly improved. After insects swallow the leaves which absorb the insecticide, active ingredients in the insecticide directly act on fat bodies and midgut nuclei of insect larvae, are rapidly replicated to cause the death of the larvae, and can induce epidemic diseases in populations by lateral infection and kill pupae and eggs through vertical infection in order to effectively control pest populations and their hazards.

The invention discloses a fat body and application thereof in evaluating interaction between a to-be-detected protein and lipid droplets. The preparation method comprises the following steps: mixing triglyceride and phospholipid in a vortex manner, and purifying by using a two-step method to prepare the fat body consisting of a triglyceride core and a single-layer phospholipid membrane; wherein the phospholipid is 'DOPC and PI', 'DOPC and PA', 'DOPC and PS', 'DOPC, PI and DOPE', 'DOPC, PA and DOPE' or 'DOPC, PS and DOPE'. Meanwhile, it is found that the fat body is specifically used as follows: detecting the structure, the form and / or the density of the to-be-detected protein on the lipid droplets; evaluating the interaction between the to-be-detected protein and the lipid droplets, detecting the affinity of the to-be-detected protein and the lipid droplets, detecting ATGL enzyme activity, analyzing the influence of the position and / or type of the amino acid residue in the to-be-detected protein on the positioning of the to-be-detected protein on the lipid droplets, and analyzing the influence of the phospholipid component on the positioning of the to-be-detected protein on the lipid droplets. The invention has important application value.

The invention discloses an insecticide for green vegetables. The insecticide is prepared from the following components in parts by weight: 5 to 10 parts of abamectin, 40 to 60 parts of fermenting solution, 3 to 8 parts of surfactant, 2 to 10 parts of activator, and 0 to 6 parts of pure alkaline. The insecticide for the green vegetables has the beneficial effects that the limitation that the insecticide mainly enters into leaves via air pores in the leaves, hydrophilic small pores in cuticles of the leaves, and cytoplasm ectodesma of leaves is broken through, and the utilization rate is greatlyincreased; after an insect eats the leaves which absorb the insecticide, the active components in the insecticide directly act onto the corpus adiposum and midgut cell of insect larvae, and are quickly duplicated to cause the disease infection and death of the larvae; the pest population and injury can be effectively controlled by transversely infecting in the populations to cause epidemic diseases and longitudinally infecting to kill pupae and ova.

The invention discloses Chinese herbal medicine weight-losing tea. The Chinese herbal medicine weight-losing tea is prepared from the following raw materials in parts by weight: 50 to 70 parts of folium nelumbinis, 5 to 15 parts of raw fructus crataegi, 5 to 15 parts of raw semen coicis, 3 to 9 parts of pericarpium citri reticulatae and 0 to 10 parts of honey. The Chinese herbal medicine weight-losing tea disclosed by the invention takes common Chinese herbal medicines capable of being both used as medicines and foods as the raw materials so that the Chinese herbal medicine weight-losing tea is safe and reliable, has no toxic side effect and has no harms to a digestion system and an absorption system of a human body; the raw materials are matched and the medical effect of each Chinese herbal medicine component can be sufficiently expressed; the Chinese herbal medicine weight-losing tea has the effects of regulating qi-flowing for eliminating phlegm and invigorating spleen to remove dampness; the honey is mainly used for seasoning and is added according to preferences of patients. The Chinese herbal medicine weight-losing tea disclosed by the invention can be used for promoting normal metabolism of the human body and has a remarkable weight-losing effect; the Chinese herbal medicine weight-losing tea has very good dampness-removing and phlegm-eliminating, and liver-soothing andspleen-tonifying effects on fatty liver patients with clinical manifestation symptoms including fat bodies, chest dull pain, drowsiness, fatigue and the like.

The invention discloses traditional Chinese medicine health tea. The traditional Chinese medicine health tea is prepared from the following raw materials in parts by weight: 50-70 parts of roses, 50-70 parts of jasmine flowers, 50-70 parts of seville orange flowers, 50-70 parts of lotus leaves, 30-50 parts of wolfberry fruits and 5-20 parts of dried orange peel. The traditional Chinese medicine health tea adopts common medicinal and edible traditional Chinese medicines as raw materials, is safe and reliable, has no toxic and side effects and no injury to the digestive system and the absorptionsystem of a human body, can give full play to medicinal effects of every traditional Chinese medicine component by compatible application of the raw materials and has the efficacies of beautifying skin, reducing lipid, producing a refreshing effect, regulating the flow of qi, relieving stagnation, resolving phlegm and removing dampness by diuresis. Honey in the traditional Chinese medicine healthtea is mainly used for flavoring and added on the preference of drinkers. The traditional Chinese medicine health tea is capable of promoting normal metabolism of a human body, has obvious weight reducing effects and also has good efficacies of regulating the flow of qi, relieving stagnation, resolving phlegm and removing dampness by diuresis on patients who have fatty liver disease due to accumulation of phlegm-dampness in the body and have the following clinical manifestations: fat body, dull pain in chest and hypochondrium, dizziness, choking sensation in chest, somnolence, tiredness and the like.

The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, and seed thereof, transformed with the nucleic acid molecules of the invention so as to confer disease resistance on the plant.

The invention discloses a self-degradable rice insecticide. The self-degradable rice insecticide is prepared from components in parts by weight as follows: 5-10 parts of vitex negundo seeds, 8-15 parts of neem seeds, 1-3 parts of a synergist, 1-3 parts of stemona japonica, 1-3 parts of chromolaena odorata and 3-6 parts of ageratina adenophora, wherein the synergist contains 40%-60% of ramie oil and 40%-60% of an organosilicon surfactant. The self-degradable rice insecticide has the following beneficial effects: the components play an obvious coordinating role, so that the insecticide can directly act on fat bodies and midgut cell nuclei of insect larvae and perform rapid replication to cause infection with disease and death of the larvae, meanwhile, epidemic disease can be caused in populations through horizontal infection, pupas and eggs are killed through longitudinal infection, and pest populations and harm are controlled effectively. The pest killing speed is high, and only half day or one day is needed from insecticide spray to death start of pests.

The invention relates to weight reducing tea capable of promoting urination. The weight reducing tea is made from the following raw materials in parts by weight: 5-15g of lotus leaves, 10-30g of poriacocos, 9-15g of bighead atractylodes rhizomes, 10-30g of coix seeds and 5-15g of dried orange peel. The weight reducing tea disclosed by the invention is suitable for patients suffering from obesitymainly caused by wet weight, and has notable treatment effects on symptoms of obese and fat bodies, abdominal fullness and distention, pant, weakness and the like.

The invention discloses a method for using silkworm cultured cell to express antibacterial peptide Cecropin B. The method comprises the following steps: (1) using the total RNA extracted from the fat body cells of wild silkworm chrysalis as template, adopting RT-PCR amplification to obtain wild silkworm antibacterial peptide Cecropin B gene; using 1% agarose gel electrophoresis to perform PCR product analysis to the antibacterial peptide Cecropin B gene, using a PCR purification kit of Qiagene for purification, then cloning the purified PCR product to TA vector pCR2.1 to obtain pCR2.1-Cecropin B, utilizing the dideoxynucleotide chain termination to confirm the correctness of cloned gene order; using restriction endonuclease BamHI and HindIII to digest pCR2.1-Cecropin B and obtain CecropinB genetic fragments, then cloning rhabdovirus transfer vector pBlueBacHisa in the genetic fragments to obtain reconstituted transfer vector; performing cotransfection of the reconstituted transfer vector and silkworm wild-type nuclear polyhedrosis virus BmNPV DNA in silkworm cultured cell, performing homologous recombination to generate recombinant virus; and (3) inoculating the recombinant virus containing wild silkworm antibacterial peptide Cecropin B gene in the silkworm cultured cell, infecting at 27 DEG C for three days to express the infection, and centrifuging to collect silkworm cultured cell.