61 results about "Negative regulator" patented technology

The application belongs to the technical field of genetic engineering, and relates to application of an immune negative regulatory factor NbELP1 in improving plant disease resistance. NbELP1 The gene is used as a plant immune negative regulatory factor, and negatively regulates early immune response induced by PAMP. NbELP1 Knocking out the gene can effectively improve the disease resistance of plants in response to pathogenic bacteria infection, and does not affect the normal growth of plants. NbELP1 The immune negative regulatory factor gene or its orthologous gene widely exists in various plants and is functionally conservative, is a high-quality gene resource with potential for genetic improvement of disease resistance of various plants, and has great application prospect.

The present invention relates to the field of plant biotechnology, and discloses the use of transcription factor OsEPR1 as a negative regulatory factor in increasing the content of volatile metabolites in rice, wherein the amino acid sequence of the transcription factor OsEPR1 is shown in SEQ ID NO. 41. The present invention significantly reduces the content of multiple volatile metabolites in rice grains by overexpressing the OsEPR1 gene driven by a constitutive promoter in rice, including but not limited to volatile metabolites in the lipoxygenase pathway; indicating that OsEPR1, as a negative regulatory factor, inhibits the synthesis of multiple volatile metabolites including the rice lipoxygenase pathway, and therefore the synthesis of multiple volatile metabolites in rice can be increased by knocking out the OsEPR1 gene in rice, and can be applied to the research on improving the aroma quality of rice.

The invention discloses an application of a NaARF5 gene in improving nicotine content of tobacco leaves, which comprises the following steps: the NaARF5 gene is mutated after gene editing, and the nucleotide sequence of the NaARF5 gene is shown as SEQ ID NO. 1. The nicotine negative regulatory factor NaNaARF5 gene is obtained from tobacco with gradually narrow leaves, and by mutating the gene sequence, not only can the nicotine content be increased, but also an important regulatory factor for plant growth is not influenced.

The invention provides application of an arabidopsis thaliana CPK12 gene in improvement of lead stress resistance of plants. The nucleotide sequence of the arabidopsis thaliana CPK12 gene is as shown in SEQ ID NO.1, and the CDS sequence of the arabidopsis thaliana CPK12 gene is as shown in SEQ ID NO.3. The invention also provides application of the arabidopsis thaliana CPK12 gene in improvement of lead stress resistance of the plants. The arabidopsis thaliana CPK12 gene is used for negative regulation of the lead stress tolerance of plants. According to the application, an arabidopsis thaliana CPK12 gene is adopted as a negative regulatory factor to participate in a new function of responding to lead stress of a plant, an RNA interference vector RNAi targeting the arabidopsis thaliana CPK12 gene is constructed and is transformed into wild-type arabidopsis thaliana by utilizing a genetic engineering technology, and a homozygous transgenic line with a silent CPK12 gene expression is obtained through resistance screening. Experiments prove that the resistance of a transgenic line to lead stress is remarkably enhanced, which indicates that the CPK12 gene plays a negative regulation role in a plant lead stress response pathway. The invention provides a new molecular target for regulating and controlling the heavy metal stress resistance of plants by using the CPK12 gene, and has important application value in the field of phytoremediation of heavy metal contaminated soil and in the aspect of molecular breeding of lead-resistant crop varieties.

The invention discloses a SbASR4 gene for regulating and controlling plant salt stress tolerance as well as an encoding protein and application thereof. The SbASR4 gene is derived from Sorghum baicola, and the encoded protein is simultaneously positioned in a cell membrane and a cell nucleus, does not have transcriptional activation activity, and is a negative regulation factor of plant salt stress response. The overexpression of the gene can significantly increase the sensitivity of the plant to salt and ABA, and the silence or knockout of the gene can greatly improve the salt tolerance of the plant. Mechanism research shows that the SbASR4 protein directly interacts with an SOS signal channel core kinase SOS2 in a plant body, and the SOS channel is negatively regulated through the interaction, so that the expression of a downstream ion transport gene is inhibited. The invention provides a method for improving the salt tolerance of plants by reducing the expression of SbASR4 or destroying the interaction of SbASR4 and SOS2, related genetic materials and products, and a brand new target and an effective strategy are provided for salt-resistant breeding of crops.

The application discloses soybeans GmGAG1 The application belongs to the field of crop breeding and relates to a soybean gene for regulating photosynthesis and yield traits. GmGAG1 The amino acid sequence coded by the gene is GmGAG1 protein with SEQ ID No.2. Experiments prove that the GmGAG1 protein can regulate photosynthetic capacity and / or biomass of plants: in the mutant obtained by editing the coding gene of the GmGAG1 protein, the starch content of leaves is reduced, the plant height is increased, the photosynthetic capacity is enhanced, the kernel and pod are enlarged, and the hundred-grain weight is increased. The results show that the GmGAG1 is a negative regulator of photosynthetic rate and kernel size.

The present invention belongs to the field of bio-agriculture, and particularly relates to a wheat negative regulator TaEDR2 and its application. The amino acid sequence of the protein encoded by the wheat negative regulator provided by the present invention is shown in SEQ ID NO: 1, and the nucleotide sequence of the wheat negative regulator TaEDR2 is shown in SEQ ID NO: 2. The present invention performs gene editing in the Fielder wild-type material to obtain TaEDR2 gene-edited plants that show resistance to the stripe rust fungus CYR32. The present invention determines that the TaEDR2 gene plays a negative regulatory role in wheat resistance to stripe rust, and uses this negative regulator to create wheat materials resistant to stripe rust, providing new genes and germplasm resources for wheat breeding for resistance to stripe rust.

The invention is directed to the treatment of tauopathic neurodegenerative conditions and the underlying processes thereof. Based on the discovery that Rhes acts as a negative regulator of normal tau clearance, the compositions and methods of the invention may be applied to inhibit Rhes and reduce pathogenic tau aggregation. Rhes may be disrupted by disrupting RAS2D gene expression or reducing the abundance of Rhes protein in neurons. Additionally, post-translational modifications of Rhes may be targeted, including the disruption of Rhes farnesylation by the administration of farnesyltransferase inhibitors.

This invention relates to the field of genetic engineering technology and provides a method for increasing erythromycin yield by modifying the SACE_5680 gene of *Rhodotorula sacchariformis*. The method involves deleting the GntR family SACE_5680 gene in *Rhodotorula sacchariformis* through genetic engineering, thereby obtaining a high-yielding engineered strain of *Rhodotorula sacchariformis* for erythromycin production. Erythromycin is then produced by fermentation using this high-yielding engineered strain. The nucleotide sequence of the SACE_5680 gene is shown in SEQ ID NO. 1. This invention also provides an application of the above method. In this study, the negative regulator of erythromycin biosynthesis, SACE_5680, was screened. By deleting a copy of the SACE_5680 gene on the chromosome of *Rhodotorula sacchariformis* through genetic engineering, a high-yielding strain of erythromycin can be obtained, providing technical support for increasing erythromycin fermentation yield in industrial production.

The invention discloses an application of an OsHDA701 gene in regulation and control of drought resistance of rice. According to the application of the OsHDA701 gene in regulating and controlling the drought resistance of the rice, the nucleotide sequence of the OsHDA701 gene is as shown in SEQ ID NO. 1. The rice drought resistance is specifically and negatively regulated by the OsHDA701 gene, and the rice drought resistance can be enhanced by knocking out the OsHDA701 gene or inhibiting the expression of the OsHDA701 gene. According to the application, it is clear that the OsHDA701 gene serves as a negative regulatory factor to control the drought resistance of the rice for the first time, and a new molecular target is provided for genetic improvement of the rice. By inhibiting the function of the negative regulation gene OsHDA701, the inherent stress resistance potential of the rice can be released, so that the drought resistance of the transgenic rice is enhanced, and the survival rate of the transgenic rice under drought stress is obviously higher than that of wild rice. According to the method disclosed by the invention, an OsHDA701 gene mutant plant is created through a mature gene editing technology, and the breeding process is accurate and rapid.

This invention belongs to the field of plant genetic engineering technology, specifically relating to the transcription factor BnC05.PLATZ04 gene regulating resistance to Sclerotinia sclerotinia in rapeseed and its application. A transcription factor BnC05.PLATZ04 gene that can be induced to express by Sclerotinia sclerotinia was screened, and its nucleotide sequence is shown in SEQ ID NO:1; the protein sequence encoded by this gene is shown in SEQ ID NO:2. Using Agrobacterium-mediated transformation, a CRISPR / Cas9 knockout mutant line of transcription factor BnC05.PLATZ04 was obtained. Identification with Sclerotinia sclerotinia through inoculation of the transgenic material revealed that the BnC05.PLATZ04 knockout line showed increased resistance to Sclerotinia sclerotinia, indicating that transcription factor BnC05.PLATZ04 is a negative regulator of rapeseed resistance to Sclerotinia sclerotinia, playing a negative regulatory role in this resistance. Deficient expression of this gene significantly improves the resistance of rapeseed to Sclerotinia sclerotinia.

This invention discloses the application of reducing the expression of negatively regulated genes in improving resistance to Phytophthora sojae, belonging to the field of plant genetic engineering. Research has found that the soybean genes GmTIFY5a or GmTIFY5b have a negative regulatory function on resistance to Phytophthora sojae. Knocking out GmTIFY5a or GmTIFY5b, or a combination of GmTIFY5a and GmTIFY5b, can enhance soybean resistance to Phytophthora sojae. This can be applied to improving soybean resistance to diseases caused by Phytophthora sojae or to breeding soybean varieties resistant to these diseases. In the soybean cultivar "Williams 82," a double mutant lacking both GmTIFY5a and GmTIFY5b, the accumulation of Phytophthora sojae was reduced by 50-60% compared to the wild type, the lesion infection area was reduced by 80-90%, and the survival rate was increased by 20-30%. Knocking out GmTIFY5a and GmTIFY5b significantly improved plant resistance to Phytophthora sojae. By genetically engineering the above genes to combat plant root rot caused by septicemia, we can not only provide important genetic resources for plant molecular breeding, but also provide an effective means to achieve high plant yields.

The application discloses a method for improving yield and / or lycopene content of tomato fruits, and belongs to the technical field of biotechnology.The method is to knockout or silence a negative regulator SlXLG2 gene in the tomato to improve the yield and / or lycopene content of the tomato fruits.The nucleotide sequence of the protein coding region of the SlXLG2 gene is shown as SEQ ID NO.1, and the amino acid sequence of the protein coded by the SlXLG2 gene is shown as SEQ ID NO.2.The method can obtain a tomato SlXLG2 gene editing mutant with high yield and high lycopene content, compared with a wild type WT, the mutant can promote the growth of the tomato plant, significantly improve the yield of the tomato plant and the lycopene content of the tomato fruits, and has a wide application prospect in cultivating high-yield and high-quality tomato germplasm.

The invention provides a method for improving the yield of lincomycin by modifying a streptomyces lincomycin SLCG3615 gene, which comprises the following steps: knocking out a TetR family transcriptional regulation gene SLCG3615 in the streptomyces lincomycin through a genetic engineering way to obtain a lincomycin high-yield engineering strain, and fermenting the obtained strain to produce the lincomycin. Wherein the nucleotide sequence of the SLCG3615 gene is as shown in SEQ ID NO. 1. The invention also provides an application of the SLCG3615 gene knockout modified streptomyces lincomus. The lincomycin biosynthesis negative regulator SLCG3615 is screened in the research, the SLCG3615 gene copy on the chromosome of the streptomyces lincomycin is knocked out through a genetic engineering way, a lincomycin high-yield strain can be obtained, and a technical support is provided for improving the fermentation yield of the lincomycin in industrial production.

This invention discloses the application of the apple C2H2 zinc finger protein transcription factor MdZAT1 gene in anthocyanin synthesis, belonging to the field of plant genetic engineering technology. This invention isolates a C2H2 zinc finger protein transcription factor MdZAT1 gene from apples and experimentally reveals a negative correlation between MdZAT1 gene expression and anthocyanin content in apple fruit during development. Overexpression of MdZAT1 in apple callus tissue inhibits anthocyanin synthesis; transient overexpression and silencing experiments in apple peel show that MdZAT1 is a negative regulator of anthocyanin accumulation in apple peel. Yeast one-hybrid assays, EMSA, and dual-luciferase assays verify that MdZAT1 protein binds to and negatively regulates MdMYB114, MdCHI, and MdANS. The MdZAT1 gene has practical application value in regulating anthocyanins in apple fruit, providing a basis for improving the appearance and quality of apple fruit.

The invention belongs to the technical field of biological medicines, and particularly relates to a long-acting STAT3 inhibition polypeptide and application thereof in tumor resistance. The long-acting STAT3 inhibition polypeptide is designed by taking an SH2 structural domain in a negative regulatory protein SH2 adapter protein F (Shf) of STAT3 protein as a template and combining single-point mutation and active fragment splicing and N-terminal long-acting fatty acid chain modification, the tumor inhibition effect is enhanced while template targeted affinity STAT3 protein and high selectivity to tumor cells are reserved, and the tumor inhibition effect is improved. Meanwhile, the plasma stability is enhanced, the half-life period is prolonged, and the bioavailability is improved. The long-acting STAT3 inhibitory polypeptide provided by the invention is stable in chemical property and relatively low in toxicity to normal cells, and has potential anti-tumor clinical application value and wide development prospect.

Disclosed herein are compounds that can degrade proteins having an active role in cancer progression, including CK1α (a negative regulator of the canonical Wnt pathway) and WEE1 (a G2 / M checkpoint kinase). Also disclosed herein are pharmaceutical compositions comprising the compounds, and methods of using the compounds, e.g., in the treatment of proliferative diseases such as cancers.

This invention belongs to the field of plant molecular biology and plant genetic engineering. Specifically, it discloses the application of the Nta11g04230 gene as a negative immune regulator gene in tobacco (Nicotiana tabacum L.) to improve tobacco disease resistance. Silencing the Nta11g04230 gene in tobacco significantly enhances tobacco immunity and increases resistance to Phytophthora nicotineis, making it an ideal susceptibility gene for improving tobacco resistance. Experimental verification shows that silencing the Nta11g04230 gene increases tobacco disease resistance, produces more salicylic acid (SA), and upregulates the expression of the SA signaling pathway marker gene NtPR1; while overexpressing the Nta11g04230 gene makes tobacco more susceptible to disease, produces less SA compared to the wild type, and downregulates the expression of the SA signaling pathway marker gene NtPR1. This invention provides a new target gene for improving tobacco disease resistance breeding. Using gene editing technologies such as CRISPR-Cas9 or through transgenic methods to reduce the expression of this gene can enhance tobacco disease resistance, and thus be applied to tobacco disease resistance improvement.

The invention provides an engineered strain as well as a construction method and application thereof. Provided are engineered strains comprising modifications, the modifications comprising: (a) expression of a gene FbpC1 that reduces transfer of trehalose monomycoic acid methyltransferase (TMM) to the cell wall; (b) the expression of a gene UmaA1 of the cyclopropane mycolic acid methyltransferase family that reduces a chemical modification process that diverse the structure of mycolic acid (MA); or (c) increasing the expression of a gene PatA that negatively regulates the enzyme of the mycolic acid synthetic pathway; wherein the alteration of expression is relative to the source strain that does not comprise the modification. According to the invention, the damage of chemical or physical methods to thalli is avoided, and the method has the advantages of simple process, low cost, high conversion efficiency and good stability.

Disclosed are methods for inducing tolerance to Huanglongbing (HLB) in citrus plants. It was found that silencing negative regulators of the plant immune system will provide resistance or tolerance to diseases, particularly HLB. NPR3 is silenced using citrus tristeza virus-based plant mediated RNA interference (CTV-RNAi) to create citrus plants that are resistance to HLB. Also disclosed are CTV-RNAi vectors designed to silence negative regulators of the plant immune system.

The invention discloses a dendrobe small RNA (Ribonucleic Acid) miR421 and an application thereof. According to the application of the miR421 in regulation and control of synthesis of the plant polysaccharide, the mature sequence of the miR421 is as shown in SEQ ID NO. 1. According to the invention, miR421 belonging to dendrobium is cloned from dendrobium officinale leaves, the miR421 is used as a negative regulatory factor to participate in regulating the synthesis process of dendrobium polysaccharide, and transient expression and functional identification prove that the polysaccharide content of transgenic dendrobium is reduced when the miR421 is excessively expressed. Therefore, the miR421 has important theoretical guidance significance and application value in the fields of synthesis of secondary metabolites of orchid plants and medical application.

This invention provides a method for modifying Streptomyces lincosinate. SLCG_1540 Methods to increase lincomycin production through gene therapy involve knocking out LacI family transcriptional regulatory genes in Streptomyces lincomyces via genetic engineering. SLCG_1540 A high-yield engineered strain of lincomycin was obtained, and the obtained strain was used to produce lincomycin through fermentation; among which, SLCG_1540 The nucleotide sequence of the gene is shown in SEQ ID NO.1. This invention also provides the above-mentioned... SLCG_1540 Applications of gene knockout modified *Streptomyces lincomycin*. The advantages of this invention are: the screening of a negative regulator of lincomycin biosynthesis, SLCG_1540, and experimental findings that gene knockout of *Streptomyces lincomycin* chromosomes via genetic engineering... SLCG_1540 This invention provides technical support for increasing lincomycin fermentation yield in industrial production by generating genes that can produce high-yield strains of lincomycin.

This invention discloses a negative regulatory gene for cold tolerance in rice seedlings. OsDMY01 Haplotypes and KASP molecular markers and their applications. Genes OsDMY01 The nucleotide sequence is shown in SEQ ID NO.1. Knocking out this gene using gene editing technology significantly improved the survival rate of rice seedlings under low-temperature stress, while overexpression of this gene reduced cold tolerance, indicating that... OsDMY01 It is a negative regulator of cold tolerance in rice seedlings. OsDMY01 Two linkage disequilibrium blocks (LD BLOCK1 and LD BLOCK2) were identified in the regulatory and exon regions of the gene, each containing the favorable haplotype Hap2, which was significantly associated with cold tolerance at the seedling stage. Corresponding KASP molecular markers were developed for the favorable haplotypes, which can be used for efficient and precise screening and creation of cold-tolerant rice germplasm, providing important genetic resources and molecular tools for molecular breeding of cold-tolerant rice.

The present invention relates to a composition comprising an anti-CD20 antibody and a PIKfyve inhibitor for preventing or treating CD20 positive cancer such as B cell lymphoma. The present invention can maximize the anticancer effect of an anti-CD20 antibody, specifically, obinutuzumab, by co-administering an anti-CD20 antibody and a PIKfyve inhibitor, which is a negative regulator of a tumor cell killing mechanism thereof. In addition, by using PIKfyve as a screening target, the present invention can rapidly identify, with high reliability, combination therapy candidate agents that can synergistically enhance the direct cell death (DCD), lysosomal membrane permeabilization (LMP), and subsequent cancer cell-killing activity of anti-CD20 antibodies.

This invention belongs to the field of nanobiomedicine technology, specifically relating to a mitochondrial-targeting copper-based nanoparticle (Qu@Cu-SS31) and its application in the preparation of drugs for treating myocardial infarction. The nanoparticles consist of quercetin (Que) and copper ions (Cu²⁺). + The nanoparticles, consisting of a self-assembly of the mitochondrial-targeting peptide SS31, exhibit a uniform, near-spherical morphology with an average particle size of 17.2 ± 1.6 nm. These nanoparticles specifically target cardiomyocyte mitochondria, responsively releasing Cu²⁺. + Furthermore, Qu@Cu-SS31 restores cardiomyocyte copper homeostasis by downregulating the copper metabolism negative regulator COMMD1, the copper uptake protein CTR1, and the copper chaperone protein CCS. Based on this, Qu@Cu-SS31 exhibits remarkable SOD-like and CAT-like enzyme activities, efficiently scavenging various reactive oxygen species / reactive nitrogen species and significantly reducing oxidative stress. Simultaneously, it inhibits NLRP3 inflammasome activation and Caspase-1-mediated pyroptosis, regulates the Bcl-2 / Bax balance to inhibit apoptosis, and promotes vascular endothelial growth factor expression and angiogenesis.

Disclosed are blocking peptides and related methods, formulations, and compositions, pertaining to the field of biomedicine. Based on the regulatory mechanism by which the negative regulator TGF-β modulates the transport and degradation of STING, the use of AP3D1 as a drug target for in vitro screening of blocking agents is provided to identify the S9 regulatory site and the R26 regulatory site of AP3D1. Corresponding blocking peptides targeting the S9 regulatory site and the R26 regulatory site, respectively, were synthesized to specifically inhibit AP3D1-mediated inactivation of STING signaling by tumor microenvironment factors, thereby blocking at least one of the S9 regulatory site and the R26 regulatory site and effectively restricting STING transport and degradation. The blocking peptide can synergize with various treatment modalities, including chemotherapy, radiotherapy, PARP inhibitors, and STING agonists, significantly enhancing cGAS-STING signaling activation in tumors during therapy and achieving more potent antitumor efficacy.

This invention discloses the application of the TRIM58 gene in regulating host resistance to Mycobacterium bovis infection. A bovine macrophage cell line with TRIM58 gene knockout was constructed using CRISPR / Cas9 technology. Results showed that TRIM58 knockout significantly inhibited intracellular survival of Mycobacterium bovis and reduced infection-induced cell death; reintroduction or overexpression of TRIM58 restored or enhanced bacterial viability. Therefore, this invention is the first to demonstrate that TRIM58 is a negative regulator of host resistance to Mycobacterium bovis infection, and that inhibitors of the TRIM58 gene can be used to prepare drugs against Mycobacterium bovis infection or to treat bovine tuberculosis. This invention fills a research gap in the field of TRIM58 in bovine tuberculosis and provides an effective tool for screening and studying the mechanisms of action of anti-bovine tuberculosis drugs.

The invention provides application of potato miR828 in regulation and control of anthocyanin biosynthesis, and relates to the technical field of biology, a nucleotide sequence of a precursor sequence of the potato miR828 is as shown in SEQ ID NO.1, and a mature sequence of the potato miR828 is as shown in SEQ ID NO.2. According to the present invention, the cutting effect of the miR828 on the StMYB3 is proved through the dual luciferase and the in vivo degradation experiment; tobacco transient expression analysis experiments find that miR828 may be a negative regulatory factor for anthocyanin biosynthesis; a dual luciferase experiment shows that after the miR828 is co-injected, the activity of the StDFR promoter is reduced; a luciferase complementation experiment shows that the miR828 inhibits the formation of a compound, and the miR828 has great significance in enriching anthocyanin biosynthesis regulation ideas, crop anthocyanin biosynthesis breeding and subsequent preparation of anthocyanin-containing health-care products.

The invention discloses an application of a rice KH structural domain gene OsKRBP1 in regulation and control of rice disease resistance. The nucleotide sequence of the OsKRBP1 gene is as shown in SEQ ID No. 1 (sequence identifier number 1). The OsKRBP1 gene is knocked out from rice, and the result shows that the function deletion or weakening of the OsKRBP1 gene in the rice can significantly improve the broad-spectrum resistance of the rice to common diseases such as rice blast and bacterial streak, indicating that the OsKRBP1 gene is an important negative regulation gene for autoimmunity of the rice, and a high-resistance rice variety can be obtained by knocking out / knocking down the OsKRBP1 gene in the rice. The invention provides a simple and effective method for cultivating resistant rice, the use of pesticides is reduced, and the food safety is guaranteed.

The invention relates to the technical field of genetic engineering, and provides a method for improving the yield of lincomycin by modifying a streptomyces lincolensis SLCG3264 gene, a MarR family transcriptional regulation gene SLCG3264 in streptomyces lincolensis is knocked out through a genetic engineering way to obtain a lincomycin high-yield strain, and the obtained strain is used for fermentation production of lincomycin. Wherein the nucleotide sequence of the SLCG3264 gene is as shown in SEQ ID NO. 1. The invention also provides an application of the SLCG3264 gene knockout modified streptomyces lincomus. According to the invention, the lincomycin biosynthesis negative regulator SLCG3264 is screened, and the SLCG3264 gene on the chromosome of the streptomyces lincomycin is knocked out through a genetic engineering way, so that a lincomycin high-yield strain can be obtained, and a technical support is provided for improving the fermentation yield of the lincomycin in industrial production.