39 results about "Negative regulator" patented technology

The application belongs to the technical field of genetic engineering, and relates to application of an immune negative regulatory factor NbELP1 in improving plant disease resistance. NbELP1 The gene is used as a plant immune negative regulatory factor, and negatively regulates early immune response induced by PAMP. NbELP1 Knocking out the gene can effectively improve the disease resistance of plants in response to pathogenic bacteria infection, and does not affect the normal growth of plants. NbELP1 The immune negative regulatory factor gene or its orthologous gene widely exists in various plants and is functionally conservative, is a high-quality gene resource with potential for genetic improvement of disease resistance of various plants, and has great application prospect.

The invention discloses a SbASR4 gene for regulating and controlling plant salt stress tolerance as well as an encoding protein and application thereof. The SbASR4 gene is derived from Sorghum baicola, and the encoded protein is simultaneously positioned in a cell membrane and a cell nucleus, does not have transcriptional activation activity, and is a negative regulation factor of plant salt stress response. The overexpression of the gene can significantly increase the sensitivity of the plant to salt and ABA, and the silence or knockout of the gene can greatly improve the salt tolerance of the plant. Mechanism research shows that the SbASR4 protein directly interacts with an SOS signal channel core kinase SOS2 in a plant body, and the SOS channel is negatively regulated through the interaction, so that the expression of a downstream ion transport gene is inhibited. The invention provides a method for improving the salt tolerance of plants by reducing the expression of SbASR4 or destroying the interaction of SbASR4 and SOS2, related genetic materials and products, and a brand new target and an effective strategy are provided for salt-resistant breeding of crops.

The application discloses soybeans GmGAG1 The application belongs to the field of crop breeding and relates to a soybean gene for regulating photosynthesis and yield traits. GmGAG1 The amino acid sequence coded by the gene is GmGAG1 protein with SEQ ID No.2. Experiments prove that the GmGAG1 protein can regulate photosynthetic capacity and / or biomass of plants: in the mutant obtained by editing the coding gene of the GmGAG1 protein, the starch content of leaves is reduced, the plant height is increased, the photosynthetic capacity is enhanced, the kernel and pod are enlarged, and the hundred-grain weight is increased. The results show that the GmGAG1 is a negative regulator of photosynthetic rate and kernel size.

The invention is directed to the treatment of tauopathic neurodegenerative conditions and the underlying processes thereof. Based on the discovery that Rhes acts as a negative regulator of normal tau clearance, the compositions and methods of the invention may be applied to inhibit Rhes and reduce pathogenic tau aggregation. Rhes may be disrupted by disrupting RAS2D gene expression or reducing the abundance of Rhes protein in neurons. Additionally, post-translational modifications of Rhes may be targeted, including the disruption of Rhes farnesylation by the administration of farnesyltransferase inhibitors.

The invention discloses an application of an OsHDA701 gene in regulation and control of drought resistance of rice. According to the application of the OsHDA701 gene in regulating and controlling the drought resistance of the rice, the nucleotide sequence of the OsHDA701 gene is as shown in SEQ ID NO. 1. The rice drought resistance is specifically and negatively regulated by the OsHDA701 gene, and the rice drought resistance can be enhanced by knocking out the OsHDA701 gene or inhibiting the expression of the OsHDA701 gene. According to the application, it is clear that the OsHDA701 gene serves as a negative regulatory factor to control the drought resistance of the rice for the first time, and a new molecular target is provided for genetic improvement of the rice. By inhibiting the function of the negative regulation gene OsHDA701, the inherent stress resistance potential of the rice can be released, so that the drought resistance of the transgenic rice is enhanced, and the survival rate of the transgenic rice under drought stress is obviously higher than that of wild rice. According to the method disclosed by the invention, an OsHDA701 gene mutant plant is created through a mature gene editing technology, and the breeding process is accurate and rapid.

This invention belongs to the field of plant genetic engineering technology, specifically relating to the transcription factor BnC05.PLATZ04 gene regulating resistance to Sclerotinia sclerotinia in rapeseed and its application. A transcription factor BnC05.PLATZ04 gene that can be induced to express by Sclerotinia sclerotinia was screened, and its nucleotide sequence is shown in SEQ ID NO:1; the protein sequence encoded by this gene is shown in SEQ ID NO:2. Using Agrobacterium-mediated transformation, a CRISPR / Cas9 knockout mutant line of transcription factor BnC05.PLATZ04 was obtained. Identification with Sclerotinia sclerotinia through inoculation of the transgenic material revealed that the BnC05.PLATZ04 knockout line showed increased resistance to Sclerotinia sclerotinia, indicating that transcription factor BnC05.PLATZ04 is a negative regulator of rapeseed resistance to Sclerotinia sclerotinia, playing a negative regulatory role in this resistance. Deficient expression of this gene significantly improves the resistance of rapeseed to Sclerotinia sclerotinia.

This invention discloses the application of reducing the expression of negatively regulated genes in improving resistance to Phytophthora sojae, belonging to the field of plant genetic engineering. Research has found that the soybean genes GmTIFY5a or GmTIFY5b have a negative regulatory function on resistance to Phytophthora sojae. Knocking out GmTIFY5a or GmTIFY5b, or a combination of GmTIFY5a and GmTIFY5b, can enhance soybean resistance to Phytophthora sojae. This can be applied to improving soybean resistance to diseases caused by Phytophthora sojae or to breeding soybean varieties resistant to these diseases. In the soybean cultivar "Williams 82," a double mutant lacking both GmTIFY5a and GmTIFY5b, the accumulation of Phytophthora sojae was reduced by 50-60% compared to the wild type, the lesion infection area was reduced by 80-90%, and the survival rate was increased by 20-30%. Knocking out GmTIFY5a and GmTIFY5b significantly improved plant resistance to Phytophthora sojae. By genetically engineering the above genes to combat plant root rot caused by septicemia, we can not only provide important genetic resources for plant molecular breeding, but also provide an effective means to achieve high plant yields.

The application discloses a method for improving yield and / or lycopene content of tomato fruits, and belongs to the technical field of biotechnology.The method is to knockout or silence a negative regulator SlXLG2 gene in the tomato to improve the yield and / or lycopene content of the tomato fruits.The nucleotide sequence of the protein coding region of the SlXLG2 gene is shown as SEQ ID NO.1, and the amino acid sequence of the protein coded by the SlXLG2 gene is shown as SEQ ID NO.2.The method can obtain a tomato SlXLG2 gene editing mutant with high yield and high lycopene content, compared with a wild type WT, the mutant can promote the growth of the tomato plant, significantly improve the yield of the tomato plant and the lycopene content of the tomato fruits, and has a wide application prospect in cultivating high-yield and high-quality tomato germplasm.

The invention provides a method for improving the yield of lincomycin by modifying a streptomyces lincomycin SLCG3615 gene, which comprises the following steps: knocking out a TetR family transcriptional regulation gene SLCG3615 in the streptomyces lincomycin through a genetic engineering way to obtain a lincomycin high-yield engineering strain, and fermenting the obtained strain to produce the lincomycin. Wherein the nucleotide sequence of the SLCG3615 gene is as shown in SEQ ID NO. 1. The invention also provides an application of the SLCG3615 gene knockout modified streptomyces lincomus. The lincomycin biosynthesis negative regulator SLCG3615 is screened in the research, the SLCG3615 gene copy on the chromosome of the streptomyces lincomycin is knocked out through a genetic engineering way, a lincomycin high-yield strain can be obtained, and a technical support is provided for improving the fermentation yield of the lincomycin in industrial production.

This invention belongs to the field of plant molecular biology and plant genetic engineering. Specifically, it discloses the application of the Nta11g04230 gene as a negative immune regulator gene in tobacco (Nicotiana tabacum L.) to improve tobacco disease resistance. Silencing the Nta11g04230 gene in tobacco significantly enhances tobacco immunity and increases resistance to Phytophthora nicotineis, making it an ideal susceptibility gene for improving tobacco resistance. Experimental verification shows that silencing the Nta11g04230 gene increases tobacco disease resistance, produces more salicylic acid (SA), and upregulates the expression of the SA signaling pathway marker gene NtPR1; while overexpressing the Nta11g04230 gene makes tobacco more susceptible to disease, produces less SA compared to the wild type, and downregulates the expression of the SA signaling pathway marker gene NtPR1. This invention provides a new target gene for improving tobacco disease resistance breeding. Using gene editing technologies such as CRISPR-Cas9 or through transgenic methods to reduce the expression of this gene can enhance tobacco disease resistance, and thus be applied to tobacco disease resistance improvement.

The invention provides an engineered strain as well as a construction method and application thereof. Provided are engineered strains comprising modifications, the modifications comprising: (a) expression of a gene FbpC1 that reduces transfer of trehalose monomycoic acid methyltransferase (TMM) to the cell wall; (b) the expression of a gene UmaA1 of the cyclopropane mycolic acid methyltransferase family that reduces a chemical modification process that diverse the structure of mycolic acid (MA); or (c) increasing the expression of a gene PatA that negatively regulates the enzyme of the mycolic acid synthetic pathway; wherein the alteration of expression is relative to the source strain that does not comprise the modification. According to the invention, the damage of chemical or physical methods to thalli is avoided, and the method has the advantages of simple process, low cost, high conversion efficiency and good stability.

The invention discloses a dendrobe small RNA (Ribonucleic Acid) miR421 and an application thereof. According to the application of the miR421 in regulation and control of synthesis of the plant polysaccharide, the mature sequence of the miR421 is as shown in SEQ ID NO. 1. According to the invention, miR421 belonging to dendrobium is cloned from dendrobium officinale leaves, the miR421 is used as a negative regulatory factor to participate in regulating the synthesis process of dendrobium polysaccharide, and transient expression and functional identification prove that the polysaccharide content of transgenic dendrobium is reduced when the miR421 is excessively expressed. Therefore, the miR421 has important theoretical guidance significance and application value in the fields of synthesis of secondary metabolites of orchid plants and medical application.

This invention provides a method for modifying Streptomyces lincosinate. SLCG_1540 Methods to increase lincomycin production through gene therapy involve knocking out LacI family transcriptional regulatory genes in Streptomyces lincomyces via genetic engineering. SLCG_1540 A high-yield engineered strain of lincomycin was obtained, and the obtained strain was used to produce lincomycin through fermentation; among which, SLCG_1540 The nucleotide sequence of the gene is shown in SEQ ID NO.1. This invention also provides the above-mentioned... SLCG_1540 Applications of gene knockout modified *Streptomyces lincomycin*. The advantages of this invention are: the screening of a negative regulator of lincomycin biosynthesis, SLCG_1540, and experimental findings that gene knockout of *Streptomyces lincomycin* chromosomes via genetic engineering... SLCG_1540 This invention provides technical support for increasing lincomycin fermentation yield in industrial production by generating genes that can produce high-yield strains of lincomycin.

This invention discloses a negative regulatory gene for cold tolerance in rice seedlings. OsDMY01 Haplotypes and KASP molecular markers and their applications. Genes OsDMY01 The nucleotide sequence is shown in SEQ ID NO.1. Knocking out this gene using gene editing technology significantly improved the survival rate of rice seedlings under low-temperature stress, while overexpression of this gene reduced cold tolerance, indicating that... OsDMY01 It is a negative regulator of cold tolerance in rice seedlings. OsDMY01 Two linkage disequilibrium blocks (LD BLOCK1 and LD BLOCK2) were identified in the regulatory and exon regions of the gene, each containing the favorable haplotype Hap2, which was significantly associated with cold tolerance at the seedling stage. Corresponding KASP molecular markers were developed for the favorable haplotypes, which can be used for efficient and precise screening and creation of cold-tolerant rice germplasm, providing important genetic resources and molecular tools for molecular breeding of cold-tolerant rice.

This invention belongs to the field of nanobiomedicine technology, specifically relating to a mitochondrial-targeting copper-based nanoparticle (Qu@Cu-SS31) and its application in the preparation of drugs for treating myocardial infarction. The nanoparticles consist of quercetin (Que) and copper ions (Cu²⁺). + The nanoparticles, consisting of a self-assembly of the mitochondrial-targeting peptide SS31, exhibit a uniform, near-spherical morphology with an average particle size of 17.2 ± 1.6 nm. These nanoparticles specifically target cardiomyocyte mitochondria, responsively releasing Cu²⁺. + Furthermore, Qu@Cu-SS31 restores cardiomyocyte copper homeostasis by downregulating the copper metabolism negative regulator COMMD1, the copper uptake protein CTR1, and the copper chaperone protein CCS. Based on this, Qu@Cu-SS31 exhibits remarkable SOD-like and CAT-like enzyme activities, efficiently scavenging various reactive oxygen species / reactive nitrogen species and significantly reducing oxidative stress. Simultaneously, it inhibits NLRP3 inflammasome activation and Caspase-1-mediated pyroptosis, regulates the Bcl-2 / Bax balance to inhibit apoptosis, and promotes vascular endothelial growth factor expression and angiogenesis.

Disclosed are blocking peptides and related methods, formulations, and compositions, pertaining to the field of biomedicine. Based on the regulatory mechanism by which the negative regulator TGF-β modulates the transport and degradation of STING, the use of AP3D1 as a drug target for in vitro screening of blocking agents is provided to identify the S9 regulatory site and the R26 regulatory site of AP3D1. Corresponding blocking peptides targeting the S9 regulatory site and the R26 regulatory site, respectively, were synthesized to specifically inhibit AP3D1-mediated inactivation of STING signaling by tumor microenvironment factors, thereby blocking at least one of the S9 regulatory site and the R26 regulatory site and effectively restricting STING transport and degradation. The blocking peptide can synergize with various treatment modalities, including chemotherapy, radiotherapy, PARP inhibitors, and STING agonists, significantly enhancing cGAS-STING signaling activation in tumors during therapy and achieving more potent antitumor efficacy.

This invention discloses the application of the TRIM58 gene in regulating host resistance to Mycobacterium bovis infection. A bovine macrophage cell line with TRIM58 gene knockout was constructed using CRISPR / Cas9 technology. Results showed that TRIM58 knockout significantly inhibited intracellular survival of Mycobacterium bovis and reduced infection-induced cell death; reintroduction or overexpression of TRIM58 restored or enhanced bacterial viability. Therefore, this invention is the first to demonstrate that TRIM58 is a negative regulator of host resistance to Mycobacterium bovis infection, and that inhibitors of the TRIM58 gene can be used to prepare drugs against Mycobacterium bovis infection or to treat bovine tuberculosis. This invention fills a research gap in the field of TRIM58 in bovine tuberculosis and provides an effective tool for screening and studying the mechanisms of action of anti-bovine tuberculosis drugs.

The invention discloses an application of a rice KH structural domain gene OsKRBP1 in regulation and control of rice disease resistance. The nucleotide sequence of the OsKRBP1 gene is as shown in SEQ ID No. 1 (sequence identifier number 1). The OsKRBP1 gene is knocked out from rice, and the result shows that the function deletion or weakening of the OsKRBP1 gene in the rice can significantly improve the broad-spectrum resistance of the rice to common diseases such as rice blast and bacterial streak, indicating that the OsKRBP1 gene is an important negative regulation gene for autoimmunity of the rice, and a high-resistance rice variety can be obtained by knocking out / knocking down the OsKRBP1 gene in the rice. The invention provides a simple and effective method for cultivating resistant rice, the use of pesticides is reduced, and the food safety is guaranteed.

The invention discloses application of a rice gene OsWAK28 in improving brown planthopper resistance of rice and a method. The gene is a DNA (Deoxyribose Nucleic Acid) sequence in SEQ ID No.1. It is found for the first time that the gene is related to rice insect resistance, and the resistance of rice to brown planthopper can be enhanced by knocking out the OsWAK28 gene in rice. The result shows that the OsWAK28 is a negative regulation factor for regulating the resistance of the rice to the brown planthopper. The method can be applied to genetic breeding of crops, and is particularly widely applied to improvement of insect-resistant breeding of rice.

This invention discloses tomatoes SlARP6 The application of genes as negative regulators in improving the cold tolerance of tomatoes, the aforementioned SlARP6 The nucleotide sequence of the protein-coding region of the gene is shown in SEQ ID NO: 1, and the application method is through knockout. SlARP6 The gene significantly enhances the low-temperature resistance of tomatoes. This invention provides key gene resources for the breeding of new low-temperature resistant tomato varieties, clarifying... SlARP6 This study explores the application value of genes as negative regulators of low-temperature resistance; it also lays an important theoretical foundation for elucidating the molecular pathways of tomato's response to stress signals and the molecular mechanisms of tolerance to adverse environments, and provides new ideas for molecular breeding practices to improve plant stress resistance.

This invention provides a method for modifying Streptomyces lincosinate. SLCG_3615 A method to increase lincomycin production through gene therapy involves knocking out the TetR family transcriptional regulatory genes in Streptomyces lincomyces via genetic engineering. SLCG_3615 A high-yield engineered strain of lincomycin was obtained, and the obtained strain was used to produce lincomycin through fermentation; among which, SLCG_3615 The nucleotide sequence of the gene is shown in SEQ ID NO.1. This invention also provides... SLCG_3615 Applications of gene knockout modified *Streptomyces lincomycin*. In this study, the negative regulator of lincomycin biosynthesis, SLCG_3615, was screened and knocked out on the chromosome of *Streptomyces lincomycin* via genetic engineering. SLCG_3615 SLCG_3615 Gene copying can yield high-yield strains of lincomycin, providing technical support for increasing the fermentation yield of lincomycin in industrial production.

The gene PBL1 for regulating rice plant type and panicle type and its application, by finding and utilizing specific proteins (such as SEQ ID NO: 2) and their encoding genes (PBL1, such as SEQ ID NO: 1), provides an effective means for precise regulation of rice plant type and panicle type. This protein acts as a negative regulator and plays a key role in regulating rice growth, helping to achieve optimization of rice plant type and improvement of panicle type, thereby improving rice yield and quality.

This invention relates to the field of plant genetic engineering technology, specifically to the application of HSFA1 protein or its encoding gene in regulating plant mesocotyl growth. This invention provides the application of the plant HSF family transcription factor HSFA1 in regulating the temperature-responsive growth of plant mesocotyls. HSFA1 is a negative regulator of the high-temperature response of mesocotyls. Under high environmental temperatures, the mesocotyl length of HSFA1 knockout mutants is significantly longer than that of the wild type, exhibiting greater sensitivity to high environmental temperatures. This has important application value in direct-seeded rice production under high-temperature conditions, providing new genetic resources and methods for breeding rice varieties suitable for direct seeding.

The application belongs to the field of plant genetic engineering, and relates to crop genetic breeding, and particularly relates to application and methods of a negative regulation factor ZmFRKH-1 in regulating drought tolerance of corn. ZmFRKH-1 (gene number: Zm00001d029973) encodes a KH domain-containing RNA binding protein, and the protein is located in the corn nucleus and cell membrane; the gene is a negative regulation factor of corn drought stress. The complete and perfect gene cloning, vector construction, genetic transformation and phenotype identification system provided in the application can be used for cultivating new drought-resistant corn varieties, creating drought-sensitive test materials, and provides important gene resources and theoretical support for analyzing the molecular regulation network of corn drought and the function research of KH domain proteins.

This invention belongs to the field of plant molecular biology technology, specifically relating to a negative regulatory transcription factor for rapeseed flowering. BnMYB615 Cloning methods BnMYB615 Gene overexpression vectors, BnMYB615 Applications of gene knockout vectors, host cells, transgenic plants with delayed flowering, and gene-edited plants with advanced flowering. The nucleotide sequence of the rapeseed transcription factor BnMYB615 is shown in SEQ ID NO.1, the amino acid sequence in SEQ ID NO.2, the overexpression vector sequence in SEQ ID NO.3, and the gene knockout vector sequence in SEQ ID NO.4. Overexpression... BnMYB615 It can significantly delay the flowering time of plants. When applied to crops that are harvested from vegetative organs, it can extend their growing season, prolong the photosynthetic time, and obtain higher yields. BnMYB615 Gene knockout advances the flowering time of plants. When applied to crops that are harvested from seeds or fruits, it can shorten their growth period, increase the multiple cropping index, avoid the effects of adverse weather, and improve product quality.

This invention discloses the application of the OsCAX1a gene in the breeding of cold-adapted rice varieties, belonging to the field of plant genetic engineering. The amino acid sequences of the two different spliceosome translation proteins encoded by the OsCAX1a gene are shown in SEQ ID NO.1 and SEQ ID NO.2, respectively, and the CDS sequences are shown in SEQ ID NO.3 and SEQ ID NO.4, respectively. This invention reveals that OsCAX1a is a negative regulator of rice cold stress adaptation. Knocking out the OsCAX1a gene inhibits rice growth and development in the early stages, but as the heading stage lengthens and the plant enters the low-temperature growth period, the mutant exhibits significantly better cold adaptation, ultimately manifested in an increased number of tillers and increased grain yield per plant. By selecting varieties with low expression levels of this gene or by mutating the gene through gene editing, superior cold-adapted rice varieties tolerant to low-temperature stress can be cultivated, showing promising application prospects.

This invention discloses a novel application and method of the previously reported use of the fig FcCAM1 gene in negatively regulating plant cold tolerance. Existing technologies only disclose the gene sequence and low-temperature induced expression pattern, but do not reveal its actual function. This invention, for the first time, demonstrates through transgenic functional experiments that overexpression of the FcCAM1 gene significantly reduces the cold tolerance of tobacco. This is manifested in increased lodging, wilting, and yellowing of overexpressing plants after low-temperature treatment, deepened leaf oxidative damage, increased relative conductivity and malondialdehyde content, and decreased SOD and CAT activities, as well as decreased soluble sugar and soluble protein content. These results indicate that the FcCAM1 gene is a negative regulator of cold tolerance. Based on this, the cold tolerance of plants can be identified or screened by detecting the expression level of the FcCAM1 gene. This invention overcomes the limitations of existing technologies and provides important gene resources and screening methods for the genetic improvement of plant cold tolerance.

This invention discloses lncRNA ABAC1 This invention relates to the application of improving the drought resistance of oats and falls under the field of plant genetic engineering technology. It is the first time that long non-coding RNA has been identified from oats. ABAC1 The nucleotide sequence of this lncRNA, as shown in SEQ ID NO.5, confirms that it is a negative regulator of drought resistance in oats, and its expression is significantly inhibited by drought stress. This was achieved by constructing the lncRNA... ABAC1 Oat transgenic lines with significantly downregulated expression of the gene were obtained by transforming oats with an RNAi silencing vector. Drought phenotype, water loss rate, and survival rate assays showed that silencing lncRNA... ABAC1 This invention can significantly reduce the rate of water loss in oat plants, improve survival rate under drought stress, and alleviate wilting damage, thereby significantly enhancing drought resistance. It provides novel gene targets and regulatory strategies for molecular breeding of drought-resistant oat varieties, and can be used for the breeding of new drought-resistant oat varieties, possessing significant theoretical value and application prospects.

The invention belongs to the technical field of plant biology, and particularly relates to application of a rice OsANR1 gene in regulation and control of false smut resistance. According to the invention, the OsANR1 gene is screened and identified from rice, and is used for coding an amino acid sequence shown as SEQ ID NO: 2. Results of embodiments show that after rice is infected by ustilaginoidea virens, the expression quantity of the OsANR1 gene is reduced, and after the OsANR1 gene is knocked out through CRISPR / Cas9, the resistance of an OsANR1 mutant strain to the ustilaginoidea virens is remarkably enhanced. OsANR1 is a negative regulatory factor of rice for resistance response to false smut, and a new genetic resource is provided for green prevention and control and molecular improvement of false smut.

This invention discloses a method for inducing mouse embryonic stem cells to transition to a pluripotent state using H2BC21 and its application, belonging to the fields of molecular biology and stem cell engineering technology. By constructing an interference vector targeting the H2BC21 gene, packaging it with lentivirus, and infecting mouse embryonic stem cells, the expression of the H2BC21 gene is knocked down, and embryonic stem cell lines with stable low expression of H2BC21 are obtained through screening. This invention is the first to discover that H2BC21 is a core negative regulator of mammalian embryonic stem cell pluripotency. After knocking down H2BC21, the expression of pluripotency core genes Nanog, Oct4, and Sox2 is downregulated, while the expression of pluripotency core genes Zscan4b, Usp17la, and Tmem92 is upregulated. The cells exhibit molecular characteristics similar to 2-cell phase cells, successfully achieving the transition from pluripotency to a pluripotent state.