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Rice disease-resistant related gene OsDR10 and its application in rice disease resistance

A rice and gene technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve problems such as unclearness, decline in yield and quality, and changes in rice disease resistance phenotypes.

Inactive Publication Date: 2008-04-09
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although many disease resistance-related genes have been identified in rice (Zhou et al., 2002; Chu et al., 2004), the mechanism of action of these genes in the rice disease resistance response, and whether a single disease resistance-related gene can cause rice disease resistance phenotype changes are not clear
[0007] Rice is an important food crop in the world, but the impact of diseases often leads to the decline of its yield and quality

Method used

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  • Rice disease-resistant related gene OsDR10 and its application in rice disease resistance
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  • Rice disease-resistant related gene OsDR10 and its application in rice disease resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Analysis of the expression pattern of OsDR10 gene in different rice varieties

[0026]In the previous research work of the present invention, the State Key Laboratory of Crop Genetic Improvement of the applicant used cDNA chip technology to analyze and found that the expression level of cDNA clone BI71N2 derived from rice variety Minghui 63 was expressed after rice variety IRBB10 was inoculated with bacterial blight strain PXO86 2.4 times lower than the control (Zhou et al., 2002). The applicant of the present invention sequenced the insert fragment of the BI71N2 clone and obtained a 690 bp cDNA sequence. The applicant used the BLASTN analysis method (Altschul et al., 1997) to search the expression tag (expressed sequence tag, EST) sequence database (Zhang et al., 2005) of the rice variety Minghui 63 with the cDNA sequence of BI71N2 clone, and found that the two segments were of different lengths The sequence of the cDNA clone EI87G08 is completely consisten...

Embodiment 2

[0029] Example 2: Isolation and clone OsDR10 gene and gene structure analysis

[0030] 1. Prediction of OsDR10 gene structure

[0031] Using the cDNA sequence EI87G08 of the OsDR10 gene as a template, the nucleotide database was searched by BLAST method (Altschul et al., 1997), and a 148626 bp sequence located on chromosome 8 of the rice variety Nipponbare in the database was found (the nucleotide database GenBank (http: / / www.ncbi.nlm.nih.gov) registration number: AP004460) a sequence (from 14046 to 14662 bp) has 100% homology with the EI87G08 sequence (E value = 0), suggesting that AP004460 contains genes homologous to OsDR10, ie alleles of OsDR10.

[0032] Using GENSCAN (http: / / genes.mit.edu / GENSCAN.html), GeneFinder (http: / / genomic.sanger.ac.uk / gf / gf.shtml), Gene Feature Searches (http: / / dot.imgen .bem.tmc.edu: 9331 / ), GeneMark (http: / / genemark.biology.gatech.edu / GeneMark / hmmchoice.html) and other gene structure prediction software analysis and prediction of homologous ge...

Embodiment 3

[0037] Example 3: Analysis of OsDR10 gene coding product

[0038] The coding region of the OsDR10 gene is located at 137-436 bp of the sequence shown in SEQ ID NO: 1 in the sequence table), and encodes a protein with a length of 100 amino acids. Analyzed by BLASTP (Altschul et al., 1997) and SMART (Simple Modular Architecture Research Tool, Letunic et al., 2006), the protein does not contain any known domains and motifs, indicating that it is a New type of protein.

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Abstract

The invention relates to plant genetic engineering technical field, in particular to separation and clone of DNA sequence containing rice disease resistance related gene OsDR10 and function identification. A part of the DNA fragment of OsDR10 gene is connected with carriers expressing double strands RNA and further transferred into rice species to inhibit the expression of OsDR10 gene of rice species by transgenic technology based on RNA interference (RNAi). Genetic transformation rice with significant decreasing of OsDR10 gene enhances resistance to white leaf blight bacteria and proves that OsDR10 gene is the negative regulator in rice anti-white leaf blight reaction.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering. It specifically involves the isolation, cloning, functional verification and application of a rice disease resistance-related gene OsDR10. The gene named OsDR10 is a negative regulator in rice disease resistance response. The disease resistance of the transgenic plants that suppressed the expression of the OsDR10 gene was significantly improved. Background technique [0002] Plants are attacked by various pathogens during their growth. There are many types of plant pathogens, including viruses, bacteria, fungi, and nematodes. Pathogen invasion of plants leads to two results: (1) the pathogen successfully reproduces in the host plant, causing related diseases; (2) the host plant produces a disease-resistant response, killing the pathogen or preventing its growth. Using resistance gene resources to improve plant disease resistance is the fundamental way to prevent diseases and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82
Inventor 王石平肖文斐
Owner HUAZHONG AGRI UNIV
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