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SKIN gene silencing plasmid and transformed plant cell containing plasmid

A technology of gene silencing and plant cells, applied in the field of plant cells and transgenic plants, can solve problems such as the mechanism is not very clear

Active Publication Date: 2015-07-29
ACAD SINICA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] However, the mechanisms that regulate source-sink communication during plant growth and development are not well understood

Method used

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  • SKIN gene silencing plasmid and transformed plant cell containing plasmid
  • SKIN gene silencing plasmid and transformed plant cell containing plasmid
  • SKIN gene silencing plasmid and transformed plant cell containing plasmid

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Embodiment Construction

[0045] Materials and methods

[0046] plant material

[0047] Rice (Oryza sativa cv Tainung 67) and barley (Hordeum vulgare cv Himalaya) were used in this study. Embryo calli were induced for 5 days in Murashige & Skoog (MS) medium containing 3% sucrose and 10 mM 2,4-D (2,4-dichlorophenoxyacetic acid). For the hydroponic culture of rice seedlings, the seeds were sterilized with 1.5% NaOCl and Tween 20 for 1 h, washed thoroughly with distilled water, and placed in a Petri dish with wet filter paper at 28 °C under the condition of 14 h light / 10 h dark cycle ( unless otherwise stated) germination. SnRK1A knockout transgenic rice was previously generated (Lu et al., 2007).

[0048] Our previous study showed that sugar regulation of MYBS1 functions in the barley aleurone layer (Lu et al., 2002). Although different systems were used, SnRK1A regulation of MYBS1 function using rice embryos (Lu et al., 2007), CIPK 15 regulation of SnRK1A expression using rice suspension cells (Lee...

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Abstract

The present invention relates to a SKIN gene silencing plasmid and a transformed plant cell containing the plasmid. The SKIN gene silencing plasmid comprises a promoter, two DNA fragments derived from one DNA segment of the cDNA of the SKIN1 or the cDNA of the SKIN2 in the positive-sense direction and in the negative-sense direction respectively, and a third DNA fragment inserted between the two DNA segments. The SKIN1 is in the form of a negative regulator for regulating the growth and the yield of rice. The expression of the endogenous SKIN reduces the yield of rice. The invention also relates to a transformed plant cell containing the SKIN gene silencing plasmid and a transgenic plant.

Description

technical field [0001] The invention relates to a SKIN gene silencing plasmid and transformed plant cells and transgenic plants containing the plasmid. SKIN is a negative regulator of rice growth and yield, and reduction of endogenous SKIN expression increases rice yield. Background technique [0002] The plant life cycle is accompanied by source-sink transitions that regulate nutrient uptake and distribution during growth and development. Regulation of source-sink communication determines the pattern of carbon allocation throughout the plant and plays a key role in determining crop productivity. Most studies have focused on processes that regulate carbon supply and demand in the expression of genes involved in carbohydrate production and storage metabolism in source tissues (photosynthetic leaves and storage organs) and sink tissues (growing vegetative and reproductive tissues). However, the components of the signal transduction pathways responsible for regulating source...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N5/10
CPCC12N15/8218C12N15/8245C12N15/8261Y02A40/146
Inventor 余淑美林倩如
Owner ACAD SINICA
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