Method for increasing yield of erythromycin by virtue of saccharopolyspora erythraea SACE_3986 gene

Abstract

The invention discloses a method for increasing yield of erythromycin by virtue of a saccharopolyspora erythraea SACE_3986 gene. The method for increasing the yield of erythromycin by virtue of the saccharopolyspora erythraea SACE_3986 gene is that a saccharopolyspora erythraea erythromycin high yield engineering strain is obtained by virtue of an SACE_3986 gene with saccharopolyspora erythraea deleted in a genetic engineering way, the obtained bacterial strain can increase the yield of erythromycin when being used for fermentation, an erythromycin biosynthesis negative regulator SACE_3986 is screened under study, and an erythromycin high yield bacterial strain can be obtained by copying the SACE_3986 gene on a chromosome with saccharopolyspora erythraea deleted in the genetic engineering way, so that technical support is provided for increasing fermentation yield of erythromycin in industrial production.

Description

technical field [0001] The invention mainly relates to a method for increasing the yield of erythromycin produced by fermentation, in particular to a method for increasing the yield of erythromycin by deleting the negative regulation gene SACE_3986 on the chromosome of Saccharopolyspora erythromycetes. Background technique [0002] Secondary metabolites of actinomycetes have a wide range of uses, such as antibiotics, anticancer agents, immunomodulators, insect repellents, insect control agents [1]. Among the 23,000 biologically active secondary metabolites discovered so far, more than 10,000 are produced by actinomycetes[2]. However, the original production of these secondary metabolites is very low, and screening is required to obtain high-yielding strains for industrial production. In the past, industrial production strains were mainly obtained by random physical or chemical mutagenesis. The traditional random mutagenesis technique is not only time-consuming, but also un...

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Problems solved by technology

In 2007, Oliynyk et al. [3] The genome sequence of Saccharopolyspora NRRL23338 was reported, but there are few studies on the regulatory genes of Saccharopolyspora, so far only bldD (SACE_2077) [4] , SACE_7040 [5] 、SACE_0012 [6] and SACE_5599 [7] Regulatory gene research reports, and two invention patents we declared: SACE_3446 (application number: 201210099708.8) and SACE_7301 (application number: 201310082765.X)

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