Method for increasing long-chain biatomic acid fermentation production rate

A technology of long-chain dibasic acid and dibasic acid, which is applied in the field of improving the fermentation yield of long-chain dibasic acid, can solve the problems of many uncertain factors and heavy strain improvement workload, and achieve the reduction of alkane consumption and fermentation yield. The effect of increasing the yield and high fermentation yield

Inactive Publication Date: 2006-06-14
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0006] The above method mainly improves the fermentation yield from the improvement of strains, but the workload of strain improvement is large, there are many uncertain factors, and the fermentation yield still needs to be further improved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Take an inoculation loop of FP-UV-56 strain, spread it on a solid slant medium in a large test tube of 20×180 mm, and cultivate it at 30° C. for 48 hours.

[0023] (2) Inoculate the strain cultured in (1) into a 500 mL Erlenmeyer shaker flask equipped with 50 mL of seed medium, and cultivate for 40 hours at 30° C. on a rotary shaker at 180 rpm. The composition of the medium is (g / L): glucose 30, KH 2 PO 4 6. Yeast extract 1, corn steep liquor 1, urea 2, NaCl 1, MgSO 4 .7H 2 O 0.5, VB 1 0.1, n-tridecane (nC 13 ) 20mL / L, prepared with tap water, natural pH.

[0024] (3) In a 500mL Erlenmeyer shaker flask equipped with 50mL fermentation medium, insert 5mL of the above seed solution, and add 0.5mmol / L inhibitor 2-bromooctanoic acid (2-Bromooctanoic acid, referred to as BA) at the same time, And do 3 parallel samples, and ferment at 30° C. for 4 days on a rotary shaker at 180 rpm. The fermentation medium is (g / L): glucose 30, KH 2 PO 4 4. Yeast extract 1, cor...

Embodiment 2

[0026]According to the method of Example 1, but the inhibitor 15-methylpalmitic acid methyl ester (15-Methylpalmitic acidmethyl ester referred to as ME) is used instead of BA, and added when the shake flask is inoculated, the concentration is 1mmol / L, and the fermentation substrate is nC 14 , the addition amount is 10% (v / v), fermented for 3 days. After the fermentation, the dibasic acid and residual alkanes were measured by the method of Example 1, and the fermentation yield was calculated. The results are shown in Table 1.

Embodiment 3

[0028] According to the method of Example 1, but replace BA with inhibitor 11-bromoundecane acid (11-Bromoundecane acid is called for short BD), and add when cell culture 40 hours, concentration is 0.05mmol / L, fermentation substrate is nC 15 , the addition amount is 15% (v / v), fermented for 4 days. After the fermentation, the dibasic acid and residual alkanes were measured by the method of Example 1, and the fermentation yield was calculated. The results are shown in Table 1.

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Abstract

The invention relates to a method for improving the fermentation yield of long-chain dibasic acids. The method of the present invention uses C 10 ~C 18 Normal alkanes are used as raw materials to produce corresponding dibasic acids by microbial fermentation. The main feature is that inhibitors are added during the fermentation process to weaken the β-oxidation ability of the strain. The inhibitors include C 5 ~C 18 One or more of halogenated fatty acids, methyl fatty acids and their salts or esters, the inhibitor concentration is 0.01-5 mmol / L. The inhibitor added in the method of the invention can effectively weaken the β-oxidation ability of the bacterial strain, reduce the consumption of alkane, greatly improve the fermentation yield, and produce acid quickly, and is especially suitable for preparing long-chain dibasic acid.

Description

technical field [0001] The invention relates to a method for improving the fermentation yield of long-chain dibasic acids, which is especially suitable for producing C 10 ~C 18 The process of long-chain dibasic acids. Background technique [0002] Long-chain dibasic acid is an important fine chemical raw material, which can be used in the synthesis of spices, hot melt adhesives, engineering plastics and liquid crystals, etc., and is widely used. [0003] At present, long-chain dibasic acids are mainly produced by microbial fermentation, and the special function of microbial double-end oxidation is used to ferment normal alkanes to produce long-chain dibasic acids. According to the mechanism study of microbial oxidation of alkanes to long-chain dibasic acids, the main reaction is ω-oxidation, and the side reaction is β-oxidation, and the long-chain dibasic acids produced can be further oxidized and degraded. Although the ω-oxidation ability of domestic and foreign producti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/46
Inventor 方向晨刘树臣李淑兰范峰
Owner CHINA PETROLEUM & CHEM CORP
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