12results about How to "Improve fermentation yield" patented technology

The invention discloses a genetic engineering strain of high-yield secondary metabolite Fumagillin as well as a construction method and application of the genetic engineering strain, and the genetic engineering strain is obtained by taking aspergillus fumigatus as a chassis bacterium through hosA gene knockout or hosA catalytic active site mutation. According to the method, a hosA gene knockout strain (delta hosA) and catalytic active site mutation strains (HosAD133A, HosAH175A and HosAD210A) are constructed by means of molecular biology, and the mutant strains are subjected to liquid or solid fermentation under the culture condition of 37 DEG C, so that the yield of Fumagillin can be remarkably increased. According to the method disclosed by the invention, the inhibition on a Fumagillin biosynthetic pathway is relieved and the metabolic flux is enhanced by utilizing the activity change of histone deacetylase caused by hosA defects, and an efficient and directional production method for high-yield Fumagillin is provided, so that the method has an important industrial application value.

This invention discloses a fermentation device for edible fungi that can improve fermentation yield. It includes a fermentation chamber, with a fixed column slidably connected to one side of the top of the chamber. A sampling cylinder is fixedly connected to the bottom of the fixed column. A limiting mechanism is provided at the top of one side of the fermentation chamber. Heating plates are installed on both sides inside the fermentation chamber, and a temperature sensor is installed on one side of the top of the fermentation chamber. This invention utilizes a drive motor installed at the top of the fermentation chamber. The drive motor drives a first gear to rotate, which in turn drives a second gear on one side to rotate. The first and second gears then drive stirring rods at the bottom to rotate, and the actuating rods on the two sets of stirring rods are staggered. This allows for rapid dispersing and mixing of the edible fungi material inside the fermentation chamber, accelerating the movement between edible fungi molecules and increasing the reaction rate of the edible fungi.

The invention provides a strain for producing L-isoleucine and a construction method and application of the strain. Pck genes of the strain are knocked out, ilvBN (G20D, I21D and I22F), ilvA (V140M, F383A), lysC (T311I), hom (G378I), thrB (A20G), ppc, aspB, thrC, asd and brnFE are over-expressed, and the strain is prepared through operons Psod-ilvBN (G20D, I21D and I22F)-ilvA (V140M, F383A)-Ptuf-thrA-thrB-thrC and Psod-ppc-aspB-Ptuf

This invention belongs to the field of microbial polysaccharide fermentation and processing technology, specifically relating to a liquid sizing agent, its preparation method, and its application. This invention optimizes the fermentation process of *Sphingomonas sphingosine monophosphate* CGMCC No. 9531 to prepare a high-yield, high-viscosity fibrous sizing agent. Then, using vegetable oil and / or mineral oil as the dispersion medium, and in conjunction with a composite emulsifier, a high-concentration liquid sizing agent is prepared. This invention solves the problems of existing sizing agents being only solid powders with poor solubility and difficulty in maintaining high-concentration sizing agents stably in liquid systems. The high-concentration liquid sizing agent of this invention allows for pumping and precise control, meets product performance requirements, can be stably stored for more than 12 months, dissolves quickly, and remains uniformly stable after 72 hours of standing. The fermentation yield is increased by 25% compared to existing processes.

The application discloses a recombinant Yarrowia lipolytica engineering bacterium for synthesizing borneol from scratch, a construction method and application thereof. The recombinant Yarrowia lipolytica engineering bacterium is constructed by integrating a multi-gene tandem expression cassette containing an orange-flower base pyrophosphate synthase coding gene, a borneol base pyrophosphate synthase coding gene and a hydrolytic enzyme coding gene into a genome H1 site of Yarrowia lipolytica ATCC 201249 by CRISPR-Cas9 gene editing technology. The application first constructs the recombinant Yarrowia lipolytica engineering bacterium for synthesizing borneol from scratch by using a simple carbon source. The engineering bacterium does not need to add any terpene precursor externally, and only uses simple carbon sources such as glucose and sucrose as substrates. The borneol is synthesized from acetyl coenzyme A, a primary metabolic starting material, by a complete MVA pathway and an artificially constructed borneol-specific synthesis pathway, so that the whole biosynthesis of borneol is realized, and the limitation of the prior art on external precursors is broken through.

This invention discloses a recombinant *Escherichia coli* strain, its preparation method and application, and a method for synthesizing oryzanol A, relating to the field of bioengineering technology. The recombinant *E. coli* strain uses *E. coli* BL21(DE3) as the chassis cell and undergoes the following modifications (a) and (b): (a) knocking out the pfkA gene; or, knocking out the pfkA gene and mutating the 5′ untranslated region of the gltA gene from the sequence shown in SEQ ID NO: 1 to the sequence shown in SEQ ID NO: 2; (b) inserting exogenous genes agmA, agmB, agmC, agmE, and agmF. This recombinant *E. coli* strain exhibits rapid growth, a short fermentation cycle (within 72 hours), can utilize inexpensive and readily available culture media, and significantly increases the fermentation yield of oryzanol A compared to existing *E. coli* systems.

This invention provides a uracil-producing strain, its construction method, and its application. The strain utilizes CRIPSR / Cas9 gene editing technology to produce... E. coli On the UR14 genome, firstly by knocking out psuG Gene, black Gene, rutA Genes and upp Genes were introduced to block the breakdown of uracil; then, the uridine phosphorylase gene was overexpressed. udp and pyrimidine-5'-nucleotide nuclease gene ppnN This synergistically enhances the synthesis and accumulation of uracil; finally, overexpression of the phosphoribosyltransferase gene... pgm, Further enhancing the conversion of the byproduct 1-phosphate ribose into the precursor 5-phosphate ribose-1-pyrophosphate improves carbon utilization. The resulting strain exhibits good genetic stability and high fermentation yield, enabling stable production of uracil and demonstrating broad application prospects.

This invention provides a method for modifying Streptomyces lincosinate. SLCG_3615 A method to increase lincomycin production through gene therapy involves knocking out the TetR family transcriptional regulatory genes in Streptomyces lincomyces via genetic engineering. SLCG_3615 A high-yield engineered strain of lincomycin was obtained, and the obtained strain was used to produce lincomycin through fermentation; among which, SLCG_3615 The nucleotide sequence of the gene is shown in SEQ ID NO.1. This invention also provides... SLCG_3615 Applications of gene knockout modified *Streptomyces lincomycin*. In this study, the negative regulator of lincomycin biosynthesis, SLCG_3615, was screened and knocked out on the chromosome of *Streptomyces lincomycin* via genetic engineering. SLCG_3615 SLCG_3615 Gene copying can yield high-yield strains of lincomycin, providing technical support for increasing the fermentation yield of lincomycin in industrial production.

The application discloses a culture method for improving the fermentation yield of tacrolimus and belongs to the technical field of tacrolimus production. The culture method for improving the fermentation yield of tacrolimus comprises the following steps: inoculating Streptomyces tsukuba into a fermentation culture medium for fermentation culture to obtain a tacrolimus fermentation liquor; and performing separation and purification on the tacrolimus fermentation liquor to obtain tacrolimus; and adding macroporous adsorption resin into the fermentation culture medium according to the fermentation time during the fermentation process. The method for improving the fermentation yield of tacrolimus controls the carbon and nitrogen source concentration and pH in the fermentation process and supplements macroporous resin, improves the secondary metabolism efficiency, adsorbs tacrolimus in the fermentation liquor through the macroporous adsorption resin, reduces the negative feedback inhibition effect on the secondary metabolism of the production strain caused by high-concentration tacrolimus, and significantly improves the tacrolimus yield.

The invention discloses an expression cassette, a genetically engineered bacterium for producing ergothioneine and application of the genetically engineered bacterium. The expression cassette comprises coding genes of Ncegt1, egtEncr and egtDAfr, and the coding genes of the egtEncr and the egtDAfr are shown in the description. The amino acid sequences of Ncegt1, egtEncr and egtDAfr are respectively as shown in SEQ ID NO: 3, SEQ ID NO: 4 and SEQ ID NO: 5. The expression cassette has the advantage of being capable of improving the fermentation yield of the ergothioneine, and the genetically engineered bacterium for producing the ergothioneine can autonomously produce histidine which is one of substrates, has the advantages of being high in fermentation yield, high in production intensity and low in cost when being applied to production, and has industrial application prospects.

The invention discloses a high-efficiency expression and production process of a recombinant human interleukin-1 receptor antagonist. The expression method of the recombinant human interleukin-1 receptor antagonist comprises the following steps: (a) obtaining a host cell for expressing the recombinant human interleukin-1 receptor antagonist; (b) inoculating the host cells into a seed culture medium and activating; (c) inoculating the activated product in the step (b) into a fermentation culture medium and fermenting; and (d) inducing the host cell to express the recombinant human interleukin-1 receptor antagonist under the condition that an inducer is added when OD600 reaches 90-110.

This application relates to the use of a lysE mutant transporter in increasing L-histidine production. Specifically, this application provides a lysE mutant transporter with the following amino acid substitutions relative to the wild-type lysE transporter shown in SEQ ID NO:1: valine at position 197 is replaced by alanine, and / or serine at position 205 is replaced by alanine or glycine. This application also provides the use of the lysE mutant transporter in the preparation of L-histidine or L-histidine derivatives. Compared to strains containing wild-type lysE, strains containing the lysE mutant exhibit increased histidine production.