398results about How to "Improve fermentation yield" patented technology

L-Glutamic acid is produced by culturing a microorganism in which an expression of L-glutamic acid-export gene, a yhfK gene, is enhanced or overexpressed, in a medium to produce and cause accumulation of L-glutamic acid in the medium, and collecting L-glutamic acid from the medium.

Production of fermentation products, such as ethanol and lactic acid in biofilm reactors by microorganisms immobilised on sterilised granular sludge.

This invention relates to one method of improving fermentation efficiency of long chain diacid. In this method, normal alkane is the material, and animalcule fermentation method is adopted to produce correspondent diacid. Its major character is to add catastaltica, which includes halogeno-aliphatic acid, methyl fat acid and one or two kinds of its salt or ester, to reduce beta oxidation ability of bacterial strain. The concentration of the catastaltica is 0.01-5mmol/L. the added catastaltica can efficiently reduce beta oxidation ability of bacterial strain, lowers the alkane consumption, and improves greatly the fermentation efficiency, the acid-producing rapidity and is particularly suitable for long chain diacid.

The invention belongs to the field of biological engineering and is aimed for solving the problem of mass scale plant diseases caused by fungus. The preparing process comprises subjecting a strong of Bacillus firmus (CGMCC No.0924) to second level fermentation culture under suitable condition, then carrying out filtering, salting out and column chromatography treatment.

The invention provides a fermentation method for preparing L-lysine products, which comprises the steps: an L-lysine culture solution is produced through three-level culture fermentation, and fine particles are coated by using fractional palm fat after spray drying. In addition, the invention also provides products prepared by the method, and the retention rate of L-lysine is larger than 15% within 24 hours.

The invention provides a method for production of ethanol by continuous fermentation of immobilized yeast cells, and the method comprises the following steps: (1) a fiber material is attached to a support frame, and is filled into a bioreactor; (2) yeast cells are immobilized in the fiber material in the bioreactor; (3) a fermentation medium is added into the bioreactor to begin ethanol fermentation; (4) when glucose in the fermentation medium is consumed, a fermentation liquid flows out of the bioreactor, the ethanol is collected, the fermentation medium is added, and then the step (4) is repeated. The method uses the fiber material for immobilization of the yeast cells for continuous fermentation to produce the ethanol, the fiber material as an immobilization material is stably in property, non-toxic, and good in adsorption and mass transfer effects, can be repeatedly used, is easy to achieve continuous production of the ethanol, and in a continuous fermentation process can greatly improve the ethanol yield.

The invention discloses paenibacillus sp. Y24-1 CGMCC No. 3700 and application of flocculating agents generated by the paenibacillus to the aspect of removing suspended substances, coloring agents and heavy metal from waste water. The ion absorption rate of the paenibacillus is respectively 90.535 percent for Pb2<+>, 78.74 percent for Hg<2+>, 18.457 percent for Cu<2+>, 15.853 percent for Co<2+> and 19.365 percent for Zn<2+>. The method comprises the following steps: carrying out fermentation culture on strains; taking fermentation liquid for centrifugation; adding absolute ethyl alcohol with 1.5 times of the volume into supernatant fluid; carrying out still standing at 4 DEG C after the materials are uniformly mixed; carrying out centrifugation on separated precipitate; carrying out ethanol washing and vacuum drying on obtained precipitate to prepare the paenibacillus. The flocculation activity is between 81.7 and 83.15 percent. The invention has wide application in the aspect of removing suspended substances, coloring agents, heavy metal and the like from waste water.

The invention discloses a method for producing lipopeptide biosurfactant through fermentation. Bacillus subtilis is immobilized on a solid material and a dual-phase pH-controlled continuous batch fermentation technology is employed, so surfactant production efficiency is effectively improved.

The present invention describes an L-glutamic acid-producing bacterium which belongs to the genus Pantoea, Enterobacter, Klebsiella or Erwinia, wherein the bacterium has been modified by gene recombination to inactivate the rpoS gene. A method is also described for culturing the bacterium in a medium to cause accumulation of L-glutamic acid in the medium, and collecting L-glutamic acid from the medium.

The invention provides a method for producing long-chain dicarboxylic acid by regulating and controlling the fermentation process through redox potential.The method comprises the steps of strain activating, seed culture and fermentation acid production.A fermentation system is a four-phase fermentation system formed by thalluses, sterile air, alkane and water, an ORP electrode is introduced into the fermentation system, and two-stage OPR control fermentation is achieved to produce the long-chain dicarboxylic acid by regulating the ventilatory capacity and stirring rotating speed in combination with an oxidizing agent and a reducing agent.According to the method, the ORP electrode is introduced in the process of producing the long-chain dicarboxylic acid through microbial fermentation, so that a two-stage ORP control strategy is achieved; the fermentation process of the long-chain dicarboxylic acid is regulated, and therefore the yield of the long-chain dicarboxylic acid and the stability of the fermentation system are improved; the fermentation yield is remarkably improved compared with a method that ORP is not controlled, even the fermentation yield is 4.6 times that of the method that the ORP is not controlled, and the production intensity can reach 1.78 g.h-1.L-1.

The invention discloses a solid ocean red yeast preparation which is mainly prepared from a composite protective agent and ocean red yeast bacterial sludge according to a mass ratio of (1.5-2.2):1, wherein the cell density of ocean red yeast in the solid ocean red yeast preparation is 200-400 billion/gram, the cell activity is 70-80%, and the protective agent comprises skim milk powder and cane sugar. The invention further discloses a preparation method of the solid ocean red yeast preparation, The preparation method comprises the following steps: firstly, performing high-density cultivation on the ocean red yeast in a cultivation mode of continuous liquid flow layer ventilation, and further drying by using a vacuum freezing drying method. According to the solid ocean red yeast preparation, the survival rate of the ocean red yeast can be increased to be 70-80%, the cell density in the preparation can be 200-400 billion/gram, and the preparation can be also used in aquatic product feeds as an additive as a product of the preparation is in a solid manner, long in expiration date and convenient to transport.

The invention discloses a method for increasing yield of erythromycin by virtue of a saccharopolyspora erythraea SACE_3986 gene. The method for increasing the yield of erythromycin by virtue of the saccharopolyspora erythraea SACE_3986 gene is that a saccharopolyspora erythraea erythromycin high yield engineering strain is obtained by virtue of an SACE_3986 gene with saccharopolyspora erythraea deleted in a genetic engineering way, the obtained bacterial strain can increase the yield of erythromycin when being used for fermentation, an erythromycin biosynthesis negative regulator SACE_3986 is screened under study, and an erythromycin high yield bacterial strain can be obtained by copying the SACE_3986 gene on a chromosome with saccharopolyspora erythraea deleted in the genetic engineering way, so that technical support is provided for increasing fermentation yield of erythromycin in industrial production.

The invention provides a multifunctional rice wind fermentation tank and a process method thereof. Semi-solid rice wine is prepared from sticky rice as a primary raw material. A cone-shaped stirrer and a round partition plate are arranged in the tank body, the round partition plate is arranged below the cone-shaped stirrer, spiral stirring blades are mounted on the cone-shaped surface of the cone-shaped stirrer, a connecting rod is mounted on the cone-shaped stirrer, penetrates through the top of the tank body and is driven by a motor, the round partition plate is composed of two semi-circularpartition plates which can turn around, and any one of the semi-circular partition plates can go up and down along with a tensile rod thereon to be horizontal or vertical to the other semi-circular partition plate. The multifunctional fermentation tank which integrates sticky rice soaking, steaming and cooling and separating steps before rice wine fermentation can finish all the work in th same container by a single person, so that the labor cost is lowered, the resources are saved, the work efficiency is improved, and the product quality is ensured. Multiple tanks are connected in series, sothat hot air and waste water are fully utilized, and the energy consumption cost is lowered.

The invention discloses a method for improving the fermentation yield of hyaluronic acid (HA), and aims to solve the problems that the yield of the HA is restricted under the influence of the physiological metabolic properties of streptococcus zooepidemicus serving as production strains, while the yield of the HA produced by a microbial fermentation method is required to be improved and the production cost is required to be reduced imperatively at present. The method comprises the following steps: slant culture, seed culture, fermentation culture, first-stage culture and second-stage culture. By utilizing the method, the fermentation yield of the HA can be further improved and the fermentation comprehensive cost is reduced in the mode of combining fed-batch culture and intermittent pH stress, so that the application of the HA in the field of medicaments, makeup and food healthcare is promoted. By utilizing the method, the conventional fermentation culture mode is subverted; the problem that the yield cannot be further improved in the method for producing the HA is effectively solved; the method has important significance to improve the fermentation yield of the HA, reduce the production cost of enterprises and promote the application of the HA.

The invention discloses aureobasidium pullulans dicarboxylic acid transport protein and a recombinant vector and application thereof. Amino acid sequence of the aureobasidium pullulans dicarboxylic acid transport protein is shown as SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9 or SEQ ID NO.10 while nucleotide sequence of the same is shown as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4 and SEQ ID NO.5. Overexpression of dicarboxylic acid transport protein in aureobasidium pullulans can increase yield of polymalic acid, and no free malic acid is detected in fermentation liquor, so that dicarboxylic acid transport protein gene is related to synthetic transport of the polymalic acid, and a foundation is laid for increasing yield of the polymalic acid.

The invention discloses a method for increasing the erythromycin yield by transforming the saccharopolyspora erythraea SACE_3980 gene. In saccharopolyspora erythraea, the gene SACE_3980 is subjected to transcription regulation by genetic engineering and through TetR-deficient family to obtain an engineering strain with high erythromycin yield; and by adopting the obtained strain for fermentation production of erythromycin, the yield can be remarkably increased, and a new technical support is provided for increasing the erythromycin yield in industrial production.

The invention discloses a method for sectional regulation and control for continuous strain culture. By adopting the method, nutrient substances are continuously replenished into a fermentation liquidto maintain strain metabolism, and at the same time, the fermentation liquid is continuously led out and is fed into a serial fermentation tank for further fermentation; to increase the strain metabolism velocity, parameters such as dissolved oxygen, pH values and replenished material constitution are respectively regulated and controlled at different stages. By adopting the method, the times ofstrain culture and equipment cleaning are reduced, the production process can be simplified, and the fermentation cost can be reduced. In addition, due to sectional regulation and control on the fermentation process, the yield of rhamnolipid can be increased, the fermentation production efficiency can be improved, the product batch stability can be improved, and the method is easily applied to industrial production.

The invention screens out lactobacillus parabrevis HX12-19 for high-yield gamma-aminobutyric acid. The lactobacillus parabrevis HX12-19 is preserved in China Center for Type Culture Collection (CCTCC), the preservation number is CCTCCM2017307. The lactobacillus parabrevis HX12-19 is obtained through isolating and screening out foodstuff materials, has high safety and can be used for fields such as food; traditional fermentation and bioconversion subdivision control are adopted, bio-enzyme conversion is fully utilized to obtain a product gamma-aminobutyric acid, the production efficiency is greatly improved, the cost is reduced, the fermentation yield can be up to 235g/L, so that the gamma-aminobutyric acid has productive competitiveness; a crystalline method is adopted to purify products, the purity can be up to 98%, and the lactobacillus parabrevis HX12-19 is suitable for industrial production.