Method for production of ethanol by continuous fermentation of immobilized yeast cells

A technology for immobilizing yeast and yeast cells is applied in the field of continuous fermentation of immobilized yeast cells to produce ethanol and continuous fermentation to produce ethanol. Facilitate automated production, high mechanical strength, and reduced equipment volume

Active Publication Date: 2014-06-18
NANJING HIGH TECH UNIV BIOLOGICAL TECH RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this type of embedding carrier has the advantages of convenient molding and high immobilization density, it has low mechanical strength, weak mass transfer, and poor resistance to microbial decomposition; at the same time, it is difficult to achieve cell self-renewal due to mass transfer limitations.

Method used

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  • Method for production of ethanol by continuous fermentation of immobilized yeast cells
  • Method for production of ethanol by continuous fermentation of immobilized yeast cells
  • Method for production of ethanol by continuous fermentation of immobilized yeast cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Using glucose as raw material to carry out single-stage continuous fermentation of immobilized yeast cells

[0037] Fermentation medium components: glucose 200g / L, peptone 7g / L, yeast extract 4g / L, ammonium sulfate 4g / L, phosphate 4g / L, ferrous sulfate heptahydrate 0.1g / L, zinc sulfate heptahydrate 0.1g / L L.

[0038] First, cut activated carbon fiber cotton 1 and steel wire mesh 2 into shapes of the same size, spread them flat on the steel mesh, and roll them into a cylinder (see figure 1 ), the fibrous material layers need to be kept loose and uniform, and loaded into the reactor 5 with a height-to-diameter ratio of 4 (see figure 2 ). Add the cultured strains (logarithmic phase) to the feed tank 3, and the seed liquid flows in from the bottom of the reactor 5 through the left pump 4 at a flow rate of 10L / h. When the seed liquid is full of the reactor, turn off the left pump 4. Turn on the right side pump and the middle valve, and circulate for a fixed pe...

Embodiment 2

[0039] Embodiment 2 carries out single-stage continuous fermentation of immobilized yeast cells with cassava raw material

[0040] Cassava hydrolyzate fermentation medium components: cassava saccharification liquid (glucose concentration 245g / L), urea 0.2g / L, magnesium sulfate 0.2g / L.

[0041] At first, plant fiber (cotton cloth) 1 and steel wire mesh are fixed in the reactor 5 that height and diameter ratio are 5 by the operating method of embodiment 1 (see figure 2 ); then add the cultured strains (logarithmic phase) into the feed tank 3, the seed liquid flows in from the bottom of the reactor 5 through the left pump 4 at a flow rate of 15L / h, until the seed liquid is full of the reactor, Close the left side pump 4, open the right side pump and the middle valve, and circulate for a certain period of time until the bacterium concentration in the feed tank 3 liquid hardly declines any more. Drain the waste liquid, add the cassava hydrolyzate fermentation medium, still flow i...

Embodiment 3

[0042] Example 3 Using glucose as raw material to carry out 5-stage continuous fermentation of immobilized yeast cells

[0043] Fermentation medium components: glucose 250g / L, peptone 5g / L, yeast extract 3g / L, ammonium sulfate 4g / L, phosphate 3g / L, ferrous sulfate heptahydrate 0.05g / L, zinc sulfate heptahydrate 0.05g / L L.

[0044] First, bamboo fibers are soaked in glyoxal solution for 4 hours. After cleaning and drying, they are rolled into a bucket with steel mesh and loaded into five reactors 5 connected in series with a height-to-diameter ratio of 4 (see image 3 ). Next, add the cultured bacteria (logarithmic phase) into the feed tank 3, flow in from the bottom of the first reactor 5 with a flow rate of 15L / h through the pump 4, and then flow out from the upper end of the last reactor 5 To the feeding tank 3, the cycle is fixed for a certain period of time until the OD value of the bacteria in the feeding tank 3 is less than 1. Drain the waste liquid, add fresh ferment...

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Abstract

The invention provides a method for production of ethanol by continuous fermentation of immobilized yeast cells, and the method comprises the following steps: (1) a fiber material is attached to a support frame, and is filled into a bioreactor; (2) yeast cells are immobilized in the fiber material in the bioreactor; (3) a fermentation medium is added into the bioreactor to begin ethanol fermentation; (4) when glucose in the fermentation medium is consumed, a fermentation liquid flows out of the bioreactor, the ethanol is collected, the fermentation medium is added, and then the step (4) is repeated. The method uses the fiber material for immobilization of the yeast cells for continuous fermentation to produce the ethanol, the fiber material as an immobilization material is stably in property, non-toxic, and good in adsorption and mass transfer effects, can be repeatedly used, is easy to achieve continuous production of the ethanol, and in a continuous fermentation process can greatly improve the ethanol yield.

Description

technical field [0001] The invention belongs to the technical field of industrial biology, and relates to a method for producing ethanol by continuous fermentation, in particular to a method for producing ethanol by continuous fermentation using immobilized yeast cells. Background technique [0002] As a renewable clean energy, ethanol has become a research hotspot in the field of industrial biotechnology. The traditional ethanol fermentation process uses free cell fermentation, and the yeast continuously flows away with the fermented mash, resulting in insufficient yeast cell concentration in the fermenter, resulting in slow alcohol fermentation and longer fermentation time. [0003] In addition, in the batch fermentation process, microorganisms are in a continuously changing environment. Due to the continuous consumption of substrates and the accumulation of harmful metabolites, microorganisms cannot be in a vigorous fermentation period for a long time, and the utilization...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06C12P7/08C12N11/14C12N11/08
CPCY02E50/10
Inventor 应汉杰陈勇柳东牛欢青何颖刘庆国
Owner NANJING HIGH TECH UNIV BIOLOGICAL TECH RES INST CO LTD
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