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37 results about "Nemadectin" patented technology

Moxidectin, a macrocyclic lactone of the milbemycin class, is a semisynthetic derivative of nemadectin, which is a fermentation product of the bacterium Streptomyces cyanogriseus subsp. noncyanogenus.

Purification method for Nemadectin

The invention provides a purification method for Nemadectin. The method comprises the following steps: performing filter pressing on flocculated Nemadectin-containing fermentation liquid to obtain filter press bacteria dregs; extracting the filter press bacteria dregs by using the aqueous solution of a first solvent to obtain a Nemadectin-containing extract; stripping the extract by using a second solvent to obtain a Nemadectin-containing stripping liquid, wherein the polarity of the second organic solvent is weaker than that of the first organic solvent; concentrating the stripping extract and crystallizing to obtain the purified Nemadectin. According to the purification method for the Nemadectin, all of the adopted separation and purification means are conventional separation means in bio-pharmacy; equipment has high universality and low cost; moreover, the production operation is simple and convenient, the production operating cost is low, and the enlargement of production scale is facilitated.
Owner:NEW FOUNDER HLDG DEV LLC +2

Method for producing Nemadectin through fermenting

The invention discloses a method for producing Nemadectin through fermenting. In the Nemadectin production process through fermenting, carbohydrates are intermittently or continuously added to a fermentation liquid to maintain the total sugar concentration of the fermentation liquid at 10-40g / L in order to carry out fermenting production when the total sugar concentration of the fermentation liquid is less than 30g / L. The method enables the Nemadectin fermentation unit to be improved by above 50%, simultaneously has the advantages of simple production operation, low equipment requirements and low cost, and is a technology suitable for the industrialized production.
Owner:NEW FOUNDER HLDG DEV LLC +2

Method for preparing high-purity nemadectin

The invention discloses a method for preparing high-purity nemadectin and belongs to the field of medicinal chemistry. The nemadectin is a sixteen-member macrolide type anthelmintic antibiotic and mainly used for synthesizing moxidectin with the better anthelmintic effect. According to the method for preparing the high-purity nemadectin, the nemadectin is extracted through an ethanol-water solution, and an extract is obtained, decolorized through ion exchange resin, deproteinized and desugared through macroporous adsorption resin and finally adsorbed and separated through the appropriate macroporous adsorption resin, so that nemadectin products with the purity higher than 90% can be prepared. The method is high in pertinency through matched usage of the resin, and the prepared high-purity nemadectin has great significance in synthesis of the high-purity moxidectin.
Owner:DALIAN JOIN KING FINE CHEM CO LTD

Method used for preparing moxidectin using nemadectin fermentation broth

The invention discloses a method used for preparing moxidectin using nemadectin fermentation broth, and belongs to the synthesis technology field of compounds with condensed rings containing 4 or more heterocyclic rings. The method comprises following steps: the nemadectin fermentation broth is subjected to solid-liquid separation so as to obtain mycelium; the mycelium is immersed in ethanol so as to obtain an ethanol immersion liquid; the ethanol immersion liquid is condensed, an organic solvent with low polarity is added for extraction, and an obtained product is condensed; methanamide or a mixed solvent of methanamide and a polar solvent is added for extraction so as to obtain an extract liquor; dichloromethane is added for extraction, and an obtained mixture is condensed, and is subjected to chromatography using macroporous resin or silicagel column so as to obtain nemadectin with a purity of more than 85%; and nemadectin is subjected to upper protection, oxidation, oximation, crystallization, deprotection, and chromatography using macroporous resin or silicagel column so as to obtain moxidectin with a purity of more than 93%. The method processes advantages such as short operation time, simple operation, high overall yield, and low cost.
Owner:HEBEI YUZE CHEM TECH

Method for preparation of moxidectin

The invention relates to a method for preparation of moxidectin. The method includes subjecting Nemadectin to protective reaction, oxidation reaction, oximation reaction and deprotection reaction in order, thus obtaining moxidectin. Specifically, the protective agent employed in the protective reaction is tert-butyl dimethylchlorosilane, and after the oximation reaction, the solid form crude product obtained by the oximation reaction is subjected to crystallization, and then the deprotection reaction is carried out on the obtained crystal. The method provided by the invention increases the product content, further reduces the difficulty for follow-up utilization of macroporous resin for purification, and improves the column loading amount, reduces the number of column chromatography, maintains or even improves the purity of the final product, and lowers the production cost.
Owner:NEW FOUNDER HLDG DEV LLC +2

Method of chemically synthesizing moxidectin

The invention relates to a method of chemically synthesizing moxidectin and belongs to the field of medicinal chemistry. The method comprises the following step: carrying out a reaction on nemadectin as a raw material and 4-chlorophenoxyacetyl chloride as an upper protective agent, and preparing moxidectin through a reaction of four steps: upper protection, oxidization, oximation and deprotection, wherein the reaction sequence of oximation and deprotection can be interchanged without affecting the final reaction product. In the reaction process, the products in upper protection, oxidization and oximation can be recrystallized and extracted through methanol and the like, so that the requirements on the purity of the raw material is reduced, the operating flow is simplified and the reaction cost is lowered.
Owner:DALIAN JOIN KING FINE CHEM CO LTD

Method for extracting crude nemadectin product from nemadectin fermentation liquor

The invention relates to a method for extracting a crude nemadectin product from nemadectin fermentation liquor, which comprises the following process steps: firstly, carrying out plate frame filter and flash drying on the nemadectin fermentation liquor so as to obtain dry nemadectin dregs; then, leaching the dry nemadectin dregs by using low alcohols as a solvent, carrying out falling film concentration on leaching liquor until no liquid flows out; adding water for acidifying until the pH value of the obtained product is 4.5-5, carrying out decoloring treatment by using macroporous resins, carrying out secondary countercurrent extraction, and reextracting; and finally, carrying out resin adsorption, and crystallizing the obtained product, thereby obtaining the crude nemadectin product. According to the invention, a preparation method for increasing the yield of nemadectin, reducing the cost, and improving the quality of crystals and finished products of nemadectin is implemented, and the method is suitable for industrial production.
Owner:宁夏泰瑞制药股份有限公司

Nemadectin purification method

The invention provides a nemadectin purification method. The method comprises the steps that: (1) a nemadectin crude product is provided; (2) the nemadectin crude product is mixed with liquid-state organic salt, and filtering is carried out, such that a filtrate is obtained; (3) the obtained filtrate is mixed with C5-C20 alkane, such that washing is carried out; and separation is carried out, such that a liquid-state organic salt-phase solution is obtained; and (4) the liquid-state organic salt-phase solution obtained in the step (3) is subjected to cooling crystallization, such that nemadectin is obtained. Compared with prior arts, the method has the following advantages: defects of current methods such as resin absorption separation are overcome; the purity of obtained nemadectin is high; the method is simple and feasible; energy consumption is low; cost is low; and single processing capacity is high. The method is suitable for industrialized mass productions.
Owner:NEW FOUNDER HLDG DEV LLC +2

Method for separating and preparing high-purity Moxidectin membrane

The invention discloses a method for separating and preparing a high-purity Moxidectin membrane, and belongs to the technical field of pharmaceutical chemistry. The method comprises the following steps: performing membrane filtration on a pre-purified Nemadectin solution; after extraction with dichloromethane, removing solvents by using conventional concentration; successively performing a protection feeding reaction, an oxidation reaction, an oximation reaction and a protection removing reaction on concentrate to obtain a Moxidectin crude solution; filtering to remove impurities through a membrane separation technique; obtaining a high-purity Moxidectin concentrated solution through a membrane concentration technique. The method disclosed by the invention uses the membrane separation technique to replace column chromatography and a macroporous resin adsorption and separation method, the technique is stable, controllable, simple and practical, the industrialization is easy, the dosage of the solvents is reduced, and a high-temperature solvent removing process of a material solution is omitted, so that the degradation risk of products is reduced, the product purity, the product quality and the product yield are improved, at the same time, the energy resources are saved, and the production cost is reduced.
Owner:DALIAN JOIN KING FINE CHEM CO LTD

Method for chemical synthesis and purification of moxidectin

The invention discloses a method for chemical synthesis and purification of moxidectin, and relates to the technical field of synthetic medicines. The method comprises the following steps: (1) carrying out upper protective reaction on nemadectin to obtain an upper protective crude product; (2) carrying out oxidation reaction on the upper protective crude product to obtain an oxidized crude product; (3) carrying out chromatography purification of macroporous resin on the oxidized crude product, and concentrating to obtain an oxidized pure product; (4) carrying out deprotection reaction on the oxidized pure product to obtain a deprotected product; and (5) carrying out oximation reaction on the deprotected product, carrying out chromatography purification of macroporous resin, concentrating, filtering and drying in vacuum to obtain the moxidectin of which the purity is higher than 96% and the maximal single impurity content is lower than 0.3%. The upper protective crude product is subjected to chromatography purification of the macroporous resin after oxidized reaction; and the impurities introduced into the subsequent chemical reaction are reduced, so that the purity of the moxidectin is improved; the impurity content is reduced; the total yield of the moxidectin is improved; and the cost is reduced.
Owner:HEBEI SHENGXUE DACHENG PHARMA

Method of purifying nemadectin through crystallization

The invention provides a method of purifying nemadectin through crystallization. The method includes: taking a nemadectin crude product, adding a first nonpolar solvent, and heating for dissolving to obtain a nemadectin solution; adding a second nonpolar solvent to be turbid, and performing gradient cooling for crystallization; sequentially filtering and drying to obtain a nemadectin pure product. By using the method, the problem that the nemadectin crude product is difficult to crystallize is solved, and high-purity nemadectin crystals are obtained. The method is simple to operate, simple in equipment, low in cost, environment-friendly and convenient for industrial production.
Owner:江苏海阔生物医药有限公司 +1

Strain belonging to the genus Streptomyces and being capable of producing nemadictin and process for producing nemadictin using the strain

In the present invention, a recombination of gene groups of nemadectin aglycon biosynthesis is performed for obtaining C-13 hydroxylnemadectin, to which sugar groups can be attached, and a production strain which produces C-13 hydroxylnemadectin is produced. Further, C-13 glycosylnemadectin producing strain is prepared by introducing aveBI-BVIII genes involving glycosidation of avermectin and biosynthesis of oleandrose. As described, C-13 hydroxylnemadectin and C-13 glycosidated nemadectin can be obtained effectively by using the producing strain prepared by means of the molecular genetic technology, and improvement in the biological activity thereof can be expected.
Owner:THE KITASATO INST

Method for improving fermentation yield of nemadectin

The invention discloses a method for improving fermentation yield of nemadectin. The method comprises the following steps: A. selecting an original strain; B. carrying out slant culture to the strain; C. bottling and cultivating the strain; D. cultivating the strain in a seeding tank; and E. cultivating the strain in a fermentation tank. By refilling sodium acetate for one or more times in the middle and later periods of fermentation, the method for improving the fermentation yield of the nemadectin disclosed by the invention can be used for greatly improving the yield of the nemadectin.
Owner:HEBEI SHENGXUE DACHENG PHARMA

Method for improving nemadectin yield

The invention provides a method for improving a nemadectin yield. The method utilizes Streptomyces cyaneogriseus BJX003 with the preservation number CGMCC No. 8731 as a producing strain to realize nemadectin fermentation production. When the broth has a reducing sugar concentration less than 12-18g / L, a glucose solution containing soybean cake powder is supplied and the reducing sugar concentration is controlled in a range of 12-18g / L. The supplement solution has a glucose concentration of 300-400g / L and a soybean cake powder concentration of 75-125g / L. The method can improve a nemadectin yield to about 3400 micrograms per milliliter from about 3200 micrograms per milliliter.
Owner:牡丹江佰佳信生物科技有限公司

Method for detecting nemadectin content based on liquid chromatography external standard method

The invention discloses a method for detecting nemadectin content based on a liquid chromatography external standard method and specifically relates to the detection for the nemadectin content. The method provided by the invention comprises the following steps: precisely weighting a nemadectin sample and a nemadectin reference substance in same weight and placing into two volumetric flasks, respectively adding 95% ethyl alcohol solution and fixing the volume to a same scale, uniformly mixing and preparing into a sample solution and a reference solution, and then quantitatively and precisely weighting the sample solution and the reference solution acquired in the step a in same volume and performing high performance liquid chromatography determination, wherein the chromatographic conditions are as follows: octadecyl silane bonded silica gel column selected as a chromatographic column, column temperature at 25-35 DEG C, 85% methyl alcohol as flowing phase, flow rate at 1.0-1.5mL / min, detection wavelength of 240nm and sampling volume at 20uL. According to the invention, the nemadectin content can be accurately detected, the accuracy is high, the repeatability is high, the linear relation coefficient can reach up to 0.99, the accuracy RSD is less than or equal to 2.0%, the sensitivity is high and the result is satisfying.
Owner:HEBEI SHENGXUE DACHENG PHARMA

Separation and purification method of high-purity moxidectin

The invention provides a separation and purification method of high-purity moxidectin. The method includes the steps: performing solid-liquid separation on fermented liquid generated by streptomycin in a fermented manner; performing leaching to obtain nemadectin concentrated solution; performing upper protection, oxidation, deprotection and amination reaction on the nemadectin concentrated solution to obtain moxidectin methanol solution (MX-4); performing preparative HPLC (high-performance liquid chromatography) on the moxidectin methanol solution, performing concentration, extracting and layering, crystal, suction filtration and drying to obtain moxidectin finished products with the purity of 98%. The method is high in separation purity and product yield, low in solvent consumption, lessin operation step and suitable for commercial mass production.
Owner:JIANGSU WEI LING BIOCHEM TECH CO LTD

Strain belonging to the genus streptomyces and being capable of producing nemadictin and process for producing nemadictin using the strain

In the present invention, a recombination of gene groups of nemadectin aglycon biosynthesis is performed for obtaining C-13 hydroxylnemadectin, to which sugar groups can be attached, and a production strain which produces C-13 hydroxylnemadectin is produced. Further, C-13 glycosylnemadectin producing strain is prepared by introducing aveBI-BVIII genes involving glycosidation of avermectin and biosynthesis of oleandrose. As described, C-13 hydroxylnemadectin and C-13 glycosidated nemadectin can be obtained effectively by using the producing strain prepared by means of the molecular genetic technology, and improvement in the biological activity thereof can be expected.
Owner:THE KITASATO INST

Method for preparing high-content nemadectin

The invention discloses a method for preparing high-content nemadectin and belongs to the technical field of organic chemistry. According to the method, crystallized and purified 5-O (protecting group)-23-Oxo nemadectin or 5-O (protecting group)-23-formaldoxime nemadectin is taken as the raw material, and the high-content nemadectin is obtained through deoximation, reduction, deprotection and silicagel column separation; the HPLC purity of the obtained nemadectin is larger than or equal to 95.0A%, and content is larger than or equal to 93.0wt.%. The method has the advantages that aftertreatment is easy, column separation frequency is low, and nemadectin content is high.
Owner:内蒙古佳瑞米精细化工有限公司

Method for preparing moxidectin

The invention discloses a method for preparing moxidectin. The method sequentially comprises the following steps: with nemadectin is used as a raw material performing substitution, oxidization and de-protection reactions on allyl chlorocarbonate; The invention discloses a novel synthesis route, wherein used raw materials have characteristics of wide and sufficient source; the method has advantagesof proper cost, simple process, mild reaction conditions of each step, conventional operation, and production cost reducing.
Owner:JIANGSU WEI LING BIOCHEM TECH CO LTD

Method for preparing high-purity 5-oxy(p-nitrobenzoyl)-nemadectin

The invention discloses a method for preparing high-purity 5-oxy(p-nitrobenzoyl)-nemadectin. According to the invention, nemadectin is adopted as a reaction initial raw material, and p-nitrobenzoyl chloride is adopted as an -OH protecting reagent, such that a 5-oxy(p-nitrobenzoyl)-nemadectin crude product is prepared; the 5-oxy(p-nitrobenzoyl)-nemadectin crude product is adopted as a raw material and is mixed with a solvent, such that a solution is prepared; an eluting agent is added, and cooling crystallization is carried out, such that 5-oxy(p-nitrobenzoyl)-nemadectin with higher purity is obtained. The method provided by the invention has the advantages of short crystallization time, low cost, high product purity and high yield. With the method, a basis is provided for the preparation of high-purity moxidectin.
Owner:XINYU PHARM CO LTD

Preparation method of nemadectin

The invention discloses a preparation method of nemadectin. According to the invention, a fermentation broth used for producing nemadectin is directly dried by spraying, such that solid powder is obtained; the solid powder is extracted by using an organic solvent, the extract is filtered, and nemadectin in the extract is absorbed by using macroporous resin; nemadectin absorbed by macroporous resin is then eluted; and the eluent is extracted, concentrated, and dried, such that a nemadectin pure product is obtained. According to the invention, a direct spray-drying method is adopted upon the fermentation broth, and the obtained dried solid fermentation bacterium powder is transportation-tolerant, and is easy to store. With the method provided by the invention, the producing of nemadectin degradation product is reduced, the bacterium powder is easy to extract, and the consumption of the extraction solvent is low. The technology is simple, the cost is low, and the method is easy to operate. With the method, the prepared nemadectin has a high HPLC purity of above 90%.
Owner:CHONGQING DAXIN PHARMA +2

Method for preparing high-content nemadectin

The invention discloses a method for preparing high-content nemadectin and belongs to the technical field of organic chemistry. The method comprises the following steps: taking crystallized and purified 5-oxygen (protecting group)-23-oxo nemadectin as a raw material, and then reducing, de-protecting and separating with a silica gel column, thereby acquiring the high-content nemadectin. The HPLC purity of the acquired nemadectin is more than or equal to 95.0A% and the content is more than or equal to 93.0wt%. The method has the advantages of simple post-processing, few column separation times and high content of nemadectin.
Owner:内蒙古佳瑞米精细化工有限公司

Recombinant vector and recombinant bacterium for improving yield of macrolide antibiotics and application of recombinant vector and recombinant bacterium

The invention discloses a recombinant vector and recombinant bacteria for improving the yield of macrolide antibiotics and application, and belongs to the field of gene engineering. The purpose of the invention is to improve the yield and titer of macrolide antibiotics. A starting vector of the recombinant vector for efflux of macrolide antibiotics is pSET152T, a streptomyces bingchengensis BC-101-4 genome is used as a template to amplify a promoter and an efflux gene, and a sequence obtained by connecting the promoter and the efflux gene is integrated into the starting vector to obtain the recombinant vector. The promoter is located at the upstream of the efflux gene. Recombinant bacteria are constructed through genetic engineering transformation, in fermentation production, the feedback inhibition effect of a final product is relieved, and the yield of milbemycins, abamectin and nemadectin is increased.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI

Moxidectin intermediate and preparation method thereof, and preparation method of moxidectin

The invention discloses a moxidectin intermediate and a preparation method thereof, and a moxidectin preparation method. The preparation method of the moxidectin intermediate comprises the following steps: carrying out nucleophilic substitution reaction on nemadectin and beta-phenylacryloyl chloride, and after the reaction is finished, carrying out post-treatment to obtain the moxidectin intermediate. According to the preparation method of the moxidectin intermediate provided by the invention, beta-phenyl acryloyl chloride is adopted as a protective reagent of hydroxyl, after the reaction is finished, post-treatment (column chromatography) purification is performed, the purity of the obtained moxidectin intermediate reaches 98% or above, the yield reaches 92% or above, the cost is low, the reaction time is short, and the method is suitable for industrial production. The method is suitable for large-scale industrial safety production.
Owner:河北美荷药业有限公司

A kind of membrane separation preparation method of high-purity moxictine

The invention discloses a method for separating and preparing a high-purity Moxidectin membrane, and belongs to the technical field of pharmaceutical chemistry. The method comprises the following steps: performing membrane filtration on a pre-purified Nemadectin solution; after extraction with dichloromethane, removing solvents by using conventional concentration; successively performing a protection feeding reaction, an oxidation reaction, an oximation reaction and a protection removing reaction on concentrate to obtain a Moxidectin crude solution; filtering to remove impurities through a membrane separation technique; obtaining a high-purity Moxidectin concentrated solution through a membrane concentration technique. The method disclosed by the invention uses the membrane separation technique to replace column chromatography and a macroporous resin adsorption and separation method, the technique is stable, controllable, simple and practical, the industrialization is easy, the dosage of the solvents is reduced, and a high-temperature solvent removing process of a material solution is omitted, so that the degradation risk of products is reduced, the product purity, the product quality and the product yield are improved, at the same time, the energy resources are saved, and the production cost is reduced.
Owner:DALIAN JOIN KING FINE CHEM CO LTD

Binary product fermentation method and two kinds of fermentation products thereof

ActiveCN104694593APlay the role of pest controlDilution of preparation costsBiocideNematocidesBiotechnologyOrganic solvent
The invention discloses a binary product fermentation method and two kinds of fermentation products thereof. The fermentation method for a composition containing Nemadectin comprises the steps of culture activation, cultivation enlarging, fermental cultivation and organic solvent purification, and recrystallization, chromatography and other complex steps are not needed. The invention further provides a composition and a pesticide preparation prepared through the fermentation method and the plant disease and pest preventing application.
Owner:河北奥思德环保科技有限公司
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