Method for preparing high-purity nemadectin

Abstract

The invention discloses a method for preparing high-purity nemadectin and belongs to the field of medicinal chemistry. The nemadectin is a sixteen-member macrolide type anthelmintic antibiotic and mainly used for synthesizing moxidectin with the better anthelmintic effect. According to the method for preparing the high-purity nemadectin, the nemadectin is extracted through an ethanol-water solution, and an extract is obtained, decolorized through ion exchange resin, deproteinized and desugared through macroporous adsorption resin and finally adsorbed and separated through the appropriate macroporous adsorption resin, so that nemadectin products with the purity higher than 90% can be prepared. The method is high in pertinency through matched usage of the resin, and the prepared high-purity nemadectin has great significance in synthesis of the high-purity moxidectin.

Description

technical field

[0001] The invention relates to a method for preparing high-purity nemoctine, which belongs to the field of medicinal chemistry. Background technique

[0002] Nimoctine is a sixteen-membered macrolide anthelmintic antibiotic, which can be used to treat internal and external parasites in animals such as cattle and sheep. Nimoctine is fermented by Streptomyces couscous, and it is mainly used to synthesize moxictine with better efficacy. Therefore, high-purity nemoctine is of special significance for the preparation of high-purity moxictine.

[0003] There are fewer methods for purifying nimoctine reported in the known literature, and there are mainly the following two types:

[0004] 1. Chinese patent CN101372492A discloses a method for purification by macroporous adsorption resin column chromatography. The purity of nemoctine obtained by this method is 40%-50%, and further column chromatography purification is required in the process of synthesizing moxicti...