A kind of culture medium and culture method for Streptomyces fermentative production of Naimamicin

The technology of a fermentation medium and a culture method is applied in the field of Streptomyces fermentative production of naimomycin, which can solve the problems of carbonization of glucose, reducing the content of total sugar, affecting the quality of fermentation broth, etc., and achieving the effect of reducing the production cycle and increasing the fermentation unit.

Active Publication Date: 2017-09-12
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] 1 The fermentation level of naimamectin is low, and its fermentation unit is lower than 3000u / ml
[0004] 2 The culture medium for domestic fermentation and production of neimamicin contains glucose. After high-temperature sterilization, it is easy to cause part of the glucose to be carbonized, reduce the total sugar content, and affect the quality of the fermentation broth.
[0006] 4 The fermentation and cultivation period of domestic neimamectin is relatively long, generally above 260 hours, resulting in high energy consumption

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Seed medium: seed tank volume 1.5m 3 , the effective volume is 1m 3 .

[0068] Seed medium: soybean oil 5L, maltose 10kg, low-temperature soybean cake powder 15kg, peanut cake powder 10kg, corn steep liquor 5L, corn gluten powder 5kg, ammonium sulfate 0.5kg, light calcium carbonate 1kg.

[0069] The seed medium is sterilized and cooled to 25°C, and kept under pressure with sterile air. The quality of the sterilized seed medium is 41mg / 100ml of amino nitrogen, 50ug / ml of dissolved phosphorus, 15mg / 100ml of total sugar, and pH7.8. Then, under the protection of the flame, put the cultured Streptomyces mother bottle fermentation liquid into the seed medium according to the inoculation amount of 0.2L for cultivation, the tank pressure is 0.03-0.05MPa; the tank temperature is 28°C; the air flow rate: 0-5h , use air stirring instead of mechanical stirring; 6h~transplant: 30m 3 / h; stirring speed 60r / min. After the seed cultivation is completed, the cell concentration is 20...

Embodiment 2

[0074] Seed medium: seed tank volume 1.5m 3 , the effective volume is 1m 3 .

[0075] Seed medium: 6L corn oil, 11kg maltose, 16kg low-temperature soybean cake powder, 11kg peanut cake powder, 6L corn steep liquor, 6kg corn gluten powder, 0.6kg ammonium sulfate, and 2kg light calcium carbonate.

[0076] The seed medium was sterilized and cooled to 26°C, and kept under pressure with sterile air. The quality of the sterilized seed medium was 45mg / 100ml of amino nitrogen, 60ug / ml of dissolved phosphorus, 17mg / 100ml of total sugar, and pH7.7. Then, under the protection of the flame, put the cultured Streptomyces mother bottle fermentation liquid into the seed medium according to the inoculation amount of 0.22L for cultivation, the tank pressure is 0.03-0.05MPa; the tank temperature is 29°C; the air flow rate: 0-5h , use air stirring instead of mechanical stirring; 6h~transplant: 35m 3 / h; stirring speed 65r / min. After the seed culture is over, the cell concentration is 22%, th...

Embodiment 3

[0081] Seed medium: seed tank volume 1.5m 3 , the effective volume is 1m 3 .

[0082] Seed medium: soybean oil 7L, maltose 12kg, low-temperature soybean cake powder 17kg, peanut cake powder 12kg, corn steep liquor 7L, corn gluten powder 7kg, ammonium sulfate 0.7kg, light calcium carbonate 3kg.

[0083] The seed medium is sterilized and cooled to 27°C, and kept under pressure with sterile air. The quality of the sterilized seed medium is 51mg / 100ml of amino nitrogen, 71ug / ml of dissolved phosphorus, 20mg / 100ml of total sugar, and pH7.5. Then, under the protection of the flame, put the cultured Streptomyces mother bottle fermentation liquid into the seed medium according to the inoculum amount of 0.25L for cultivation, the tank pressure is 0.03-0.05MPa; the tank temperature is 30°C; the air flow rate: 0-5h , use air stirring instead of mechanical stirring; 6h~transplant: 40m 3 / h; stirring speed 70r / min. After the seed cultivation is completed, the cell concentration is 25%,...

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Abstract

The invention relates to a culture medium for producing nema rhzomorph by streptomyces fermentation and a cultivation method. The fermentation unit of the nema rhzomorph can be improved, the fermentation cycle can be shortened, the fermentation cost can be reduced, the effect on primary and auxiliary materials caused by the environment is reduced to the maximal extent, and adequate supply of the primary and auxiliary materials is ensured by using the culture medium formula and the fermentation control process provided by the invention, thereby achieving stable and efficient production of the nema rhzomorph.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a novel culture medium and a culture method for streptomyces fermenting and producing nemamycin. Background technique [0002] Like ivermectin, neimamectin is a broad-spectrum, high-efficiency, new macrolide anthelmintic antibiotic widely used in veterinary clinics, and is a member of the mebemycin family. At present, the domestic fermentative production of naimamectin adopts the secondary fermentation mode, and the main problems of this mode are as follows: [0003] 1 The fermentation level of naimamectin is low, and its fermentation unit is lower than 3000u / ml. [0004] 2 The culture medium for domestic fermented production of neimamicin contains glucose. After high-temperature sterilization, it is easy to cause part of the glucose to be carbonized, reduce the total sugar content, and affect the quality of the fermentation broth. [0005] 3 The procurement co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/18C12R1/465
Inventor 任勇奇乃李小萍董媛
Owner 宁夏泰瑞制药股份有限公司
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