Method for extracting crude nemadectin product from nemadectin fermentation liquor

A technology of neimamicin and fermented liquid, which is applied in the field of extracting crude neimamicin from fermented neimamicin, which can solve the problems of high extraction cost, unfavorable environmental protection, and low extraction yield, and avoid environmental pollution problems, enhancing market competitiveness, and improving extraction yield

Active Publication Date: 2014-12-10
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]1 The extraction yield is low, generally 60-70%
[0005]2 The extraction period is longer, exceeding 60h
[0006]3 The extraction cost is high, which reduces the market competitiveness of the product
[0007]4 During the extraction process, a lot of waste water is produced, which is not conducive to environmental protection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Naimamectin Fermentation Broth 10m 3 , The titer is 4123u / ml. Add 100kg of diatomaceous earth and 500L of drinking water, stir for 10 minutes, and filter with plate and frame to obtain 583kg of fungal residue. Start the flash drying equipment, control the inlet air temperature at 145-160°C, and control the outlet air temperature at 60-90°C. Put the wet fungus residue into the feeding port of the flash dryer, control the feeding speed at 100-400r / min, and carry out flash drying to obtain 496kg of dried naimamectin residue. After 2 times of leaching, the total amount of n-propanol was added to be 5000L, the potency of the extract was 7982u / ml, and the extraction yield was 96.8%, and then the solid-liquid separation. After concentration by falling film, 42.3 kg of solid neimamectin mixture was obtained. Add 423L of purified water, add 30% acetic acid to adjust the pH to 4.5, and filter, the volume of the filtrate is 438L. Use macroporous resin to decolorize the naimame...

Embodiment 2

[0036] Naimamectin Fermentation Broth 10m 3 , the titer is 4017u / ml. Add 200kg of calcium carbonate and 600L of drinking water, stir for 15min, and filter through plate and frame to obtain 678kg of fungus residue. Start the flash drying equipment, control the inlet air temperature at 145-160°C, and control the outlet air temperature at 60-90°C. Put the wet fungus residue into the feeding port of the flash dryer, control the feeding speed at 100-400r / min, and carry out flash drying to obtain 581kg of dried neimamicin residue. After 2 extractions, the total amount of ethanol added was 5300L, the titer of the extract was 7359u / ml, and the extraction yield was 97.1%, and then the solid-liquid separation. After concentration by falling film, 39 kg of solid neimamectin mixture was obtained. Add 430L of purified water, add 30% hydrochloric acid to adjust the pH to 4.6, and filter, the volume of the filtrate is 442L. Use macroporous resin to decolorize the naimamectin filtrate. T...

Embodiment 3

[0038] Naimamectin Fermentation Broth 10m 3, The titer is 4106u / ml. Add 500kg of diatomaceous earth and 700L of drinking water, stir for 20min, and filter with plate and frame to obtain 942kg of fungus residue. Start the flash drying equipment, control the inlet air temperature at 145-160°C, and control the outlet air temperature at 60-90°C. Put the wet fungus residue into the feeding port of the flash dryer, control the feeding speed at 100-400r / min, and carry out flash drying to obtain 829kg of dried neimamicin residue. After 2 extractions, the total amount of methanol added is 5500L, the potency of the extract is 7279u / ml, the extraction yield is 97.5%, and then the solid-liquid separation. After concentration by falling film, 40kg of solid neimamectin mixture was obtained. Add 480L of purified water, add 30% sulfuric acid to adjust the pH to 4.7, and filter, the volume of the filtrate is 489L. Use macroporous resin to decolorize the naimamectin filtrate. The amount of ...

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PUM

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Abstract

The invention relates to a method for extracting a crude nemadectin product from nemadectin fermentation liquor, which comprises the following process steps: firstly, carrying out plate frame filter and flash drying on the nemadectin fermentation liquor so as to obtain dry nemadectin dregs; then, leaching the dry nemadectin dregs by using low alcohols as a solvent, carrying out falling film concentration on leaching liquor until no liquid flows out; adding water for acidifying until the pH value of the obtained product is 4.5-5, carrying out decoloring treatment by using macroporous resins, carrying out secondary countercurrent extraction, and reextracting; and finally, carrying out resin adsorption, and crystallizing the obtained product, thereby obtaining the crude nemadectin product. According to the invention, a preparation method for increasing the yield of nemadectin, reducing the cost, and improving the quality of crystals and finished products of nemadectin is implemented, and the method is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of antibiotic extraction, in particular to a method for extracting crude neimamicin from a fermented neimamicin liquid. Background technique [0002] Naimamectin is an antiparasitic drug, a single-component macrolide antibiotic fermented by Streptomyces, produced internationally by Novartis and Sankyo. Neimamectin is the starting material for the synthesis of moxidectin. At present, antiparasitic drugs are widely used in basic agriculture such as animal husbandry and aquaculture, and the international demand for antiparasitic drugs has continued to grow. [0003] It is understood that the extraction process of neimamectin in China is relatively complicated, and the main technical methods are chromatography and secondary resin adsorption. The main problems are: [0004] 1 The extraction yield is low, generally 60-70%. [0005] 2 The extraction cycle is longer, exceeding 60 hours. [0006] 3 The extraction...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/22
CPCC07D493/22
Inventor 任勇奇乃李小萍董媛
Owner 宁夏泰瑞制药股份有限公司
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