353results about How to "High extraction yield" patented technology

A process for preparing the sodium hyaluronate from the flermented liquid of hyaluronic acid incldues such steps as removing bacteria from said fermented liquid, enzymolyzing, depositing in alcohol, complexing, cleavaging, depositing in alcohol, ultrafiltering, depositing in alcohol, washing, dewatering and dryin. It has high molecular weight and low content of protein.

The invention discloses a method for effectively degrading and removing pulullan polysaccharide in Aureobasidium pullulans fermentation broth by utilizing pullulanase so as to be favorable for extracting and obtaining beta-polymalic acid. The method can effectively degrade and remove the pulullan polysaccharide in the Aureobasidium pullulans fermentation broth, improve the yield and the purity of products, avoids a series of trivial operations caused by the interference of the pulullan polysaccharide, improve the working efficiency and reduce the production cost of the products. The method is realized by the following proposal: Aureobasidium pullulans is subjected to activation and seed culture and is inoculated into a fermentation medium; the Aureobasidium pullulans is cultured between 7 and 12 d at the temperature of between 24 and 30 DEG C and under the condition of between 120 and 200 r/m, fermentation broth is subjected to 5,000 r/m centrifugation or filtration after the fermentation is over, and thalli are removed; the pH value of a fermentation filtered fluid (a supernatant) is adjusted to be between 5 and 7, 6-11 plu/mL supernatant of the Pullulanase is added, and the reaction is performed for 30 to 90 minutes at the temperature of between 30 and 50 DEG C; subsequently dried methyl alcohol is added until the final concentration of methanol is between 40 and 60 percent, the mixture is slowly stirred and kept stand over night, and the obtained deposit is a coarse product of the beta-polymalic acid; and the coarse product of the beta-polymalic acid is dissolved in distilled water according to the ratio of 1:10(m:v), the obtained product is subjected to 5,000 r/m centrifugation, micromolecular undissolved substances are removed, and the beta-polymalic acid is obtained after vacuum concentration and freeze drying.

The invention discloses a method for extracting gamma-polyglutamic acid. The scheme of the method comprises the following steps: filtering out thallus by pumping fermentation liquor; adding organic solvent into fermentation supernate, adding sodium chloride, potassium chloride or ammonium sulfate, and putting for one night to obtain precipitate which is a gamma-polyglutamic acid coarse product; dissolving the gamma-polyglutamic acid coarse product into distilled water, filtering out undissolved micromolecule substance in a centrifuging way, adding sodium chloride, potassium chloride or ammonium sulfate and organic solvent into the supernate, stirring to obtain a gamma-polyglutamic acid precipitate, dissolving the gamma-polyglutamic acid precipitate into the distilled water according to 1: (5-10) (m : v), and filtering out impurity by an ultrafiltration membrane; and performing the freeze drying to ultrafiltration peritoneal effluent so as to obtain white gamma-polyglutamic acid powder. After the method is used, the product extraction yield can be effectively improved, the usage amount of the organic solvent can be reduced, the production cost can be lowered, the environment problem caused by a large number of organic solvent during extracting can be avoided, and the environment pollution can be reduced.

The invention relates to a new treatment method of threonine mother liquid. The method comprises a step of adding an alcohol substance into a threonine mother liquid solution obtained in an extraction process of threonine mother liquid so as to reduce the solubility and separate out threonine, and a compound microbial bacterial agent is added into other impurities to reach the emission standard. The method provided by the invention has the advantages of low production cost, high threonine extraction yield and little wastewater discharge, is economical and environment-friendly and has a broad application prospect.

The invention discloses a high- purity of osthol, structure proof with spectral analysis, method for preparation, stability study, acute toxicity study, the drug compositions containing osthol, and new drug utilities of the natural product. By the technique steps of extracted Chinese pharmacy conidium fruit with alcohol, decolorizing and enriching by large-hole adsorption resin, and separating and purifying, it can prepare osthol with a purity of above 98%. The study expresses that the osthol has a clear utility of anti-cancer, anti-auto lymphoma, and so on.

The invention provides application and a preparation method of tea seed episperm bioactive extracts, relating to the technical field of comprehensive utilization of agricultural waste resources. The extracts extracted from the hulls of oil tea fruits and tea fruits comprise bioactive materials, such as saccharicterpenin, tea polyphenol and the like and have the advantages of high comprehensive performance, wide and special application, wide sources and low cost; the extracts can be used as feed additives and antioxidants or used as intermediate products to respectively obtain tea saponin, tea polyphenol or tea-seed polysaccharide applied to food and pharmaceuticals industry after separating and purifying treatment; in the preparation method, the hulls of the oil tea fruits and the tea fruits are soaked in a water-containing or anhydrous organic solvent and the extracts are directly extracted after dried in vacuum, and the preparation method has the characteristics of high extracting efficiency, high utilization efficiency of raw materials, simple and convenient producing process, low cost, wide raw material sources, wide application range, capability of turning waste into wealth, environment improvement, mass production, easy realization of industrialization, high economic benefit and the like.

The invention provides an oil tea peel extract containing oil tea peel polyphenol as well as a preparation method and application of the oil tea peel extract. The preparation method of the oil tea peel extract containing the oil tea peel polyphenol comprises the following steps: (1) crushing fresh oil tea peel; (2) lixiviating the crushed peel with an organic solvent and meanwhile acting on an extraction system by ultrasonic waves; (3) separating and purifying with a nanometer film; and (4) drying in vacuum. In the invention, the oil tea peel extract containing the oil tea peel polyphenol is mixed with an emulsifying agent and a synergist, then heated and finally added to fatty oil to obtain a feed anti-oxidant additive. In the oil tea peel extract, the polyphenol content reaches up to 55-80wt% (based on gallic acid); and the feed anti-oxidant additive made by the oil tea peel extract can effectively prolong the quality guarantee period of feed and improve the utilization ratio of the feed. The preparation method has the characteristics of high extraction yield, high utilization ratio of raw materials, and simple, convenient and feasible production process, is easy to realize industrialization and the like.

The invention relates to utilization and technology of agriculture and forestry biological data, in particular to a method for separating lignin and holocellulose from wood fiber raw materials. The method comprises the following steps: a, wood fiber raw materials are mixed with an alkali liquor and then soaked and moistened; b, the soaked and moistened wood fiber raw materials are homogenized and then filtered and separated to obtain solid residues and a filtrate I; c, the solid residues which are washed with water are added into a benzene sulfonate water solution for digestion, and the digested solid residues are filtered and separated to obtain residues containing holocellulose and a filtrate containing lignin; d, the residues containing holocellulose are washed to obtain holocellulose; e, water is added into the filtrate containing lignin to separate lignin, filtering is performed to obtain lignin residues and a filtrate II, and the lignin residues are washed and dried to obtain lignin. The method provided by the invention is simple and feasible in process, environment-friendly, and low in toxicity, the lignin extraction yield is high, and the saccharification efficiency of holocellulose obtained through separation is also high.

The invention provides a method for separating the purified amygdalin from the loquat nucleus, comprising: extracting the amygdalin from the loquat nucleus to produce a concrete with a hydrophilic organic water solution; fully dissolving the concrete by ethanol, filtering, depressed dealcoholization of the filtrate, cryogenic crystallization to produce the coarse amygdalin; fully dissolving the amygdalin, filtering, cryogenic recrystallization, washing by ethanol-aether, getting a amygdalin white crystal with a purity not less than 98.7%. The invention purifies the amygdalin from the loquat nucleus which solves the problem that the amygdalin as an original medicine is hydrolyzed by acid and enzyme so as to reduce the curative effect and has a broad extension.

The present invention relates to a method for extracting vitamin B2 from vitamni B2 fermentation liquor. Said method utilizes addition of calcium chloride, disodium hydrogen phosphate and sodium polyacrylate to make flocculation, after filtration adds aqueous hydrogen peroxide solution to make precipitation and separation, then makes crystallization and recrystallization so as to obtain the invented vitamin B2 whose extracted yield is 85%-87%, and whose purity can be up to 98%-100%.

The invention discloses a dried tangerine peel extract preparation method, wherein the dried tangerine peel extract is prepared by using dried tangerine peel as a raw material through cellulase and pectinase combined enzymolysis. The invention further discloses the dried tangerine peel extract prepared by using the method and applications of the dried tangerine peel extract. According to the present invention, by using the cellulase and pectinase combined enzymolysis method, the characteristics of high efficiency, simple and convenient operation, mild reaction condition and the like can be provided; after the enzymolysis reaction, ethanol is used as a solvent to extract; and the method has advantages of high yield, safety, effectiveness and solvent recovery, and is suitable for industrial production.

The invention relates to a preparation method of acetylisovaleryltylosin tartrate. The method comprises the following steps: adjusting the pH of acetylisovaleryltylosin fermentation liquor to 1-1.5 by using oxalic acid, and standing for 20-30 minutes; adjusting the pH of acetylisovaleryltylosin back to 4.5-5.5 by using 10-15 percent liquid alkali; standing for 20-30 minutes once again; performing plate frame filtering, diluting, decolorizing, nano-filtering, crystalizing, water washing, salifying and spray drying on the fermentation liquor to obtain the acetylisovaleryltylosin tartrate. By adopting the preparation method, the conventional crystallizing process is eliminated, the yield of acetylisovaleryltylosin tartrate is increased by virtue of extracting, crystallizing and synthesizing processes, the cost is lowered, and the product quality is improved. The method is suitable for industrial production.

The invention relates to pullulanase and a production method thereof, and belongs to an amylolytic enzyme acted on an alpha-1,6-glucosidic bond. The pullulanase and the production method are characterized in that: a, zymologic characteristic is that the optimal pH is 3.8 to 4.4, the stable pH is 3.5 to 4.5, and the optimal action temperature is 50 to 65 DEG C; and b, Bacillus licheniformis is selected as an enzyme producing strain and derived from China Center of Industrial Culture Collection (CICC No.10185), and the pullulanase is prepared by a liquid state fermentation process in a mode of compositely feeding a backing material and a feed supplement and the method meets the following technological indexes that: (1) the enzymatic activity of the product is more than or equal to 1,100u/ml; (2) the fermentation period is 72 to 80 hours; and (3) the liquid enzyme recovery rate is more than or equal to 86.5 percent. The pullulanase product has the advantages of high heat resistance and acid resistance, high enzymatic activity, stable performance and low cost; the liquid biological fermentation production method has the advantages of high fermentation enzymatic activity, high extracting yield and low manufacturing cost; and the pullulanase and the production method are suitable for the fermentation industry using starch as a raw material and manufacturing of modified starch products, amylose starch and high-amylose starch.

The present invention relates to the technological process of film extracting L-phynylalanine as medicine intermediate. To L-phynylalanine solution prepared with pyenyl-pyruvic acid through enzyme process, the film separation process, including eliminating thallus and cell fragment, ultrafiltration to elimiante hybrid protein, nanometer filtering to desalt and decolor and concentrate, to separate and extract target product. The said process has L-phynylalanine yield over 85%, and compared with available extraction process including ion exchange and metal salt precipitation, the said process has higher yield, and less consumption of acid, alkali, water and steam.

An improved process for producing substantially non-polar doped or un-doped cerium oxide nanoparticle dispersions is disclosed. The cerium-containing oxide nanoparticles of an aqueous colloid are transferred to a substantially non-polar liquid comprising one or more amphiphilic materials, one or more low-polarity solvents, and one or more glycol ether promoter materials. The transfer is achieved by mixing the aqueous and substantially non-polar materials, forming an emulsion, followed by a phase separation into a remnant polar solution phase and a substantially non-polar organic colloid phase. The organic colloid phase is then collected. The promoter functions to speed the transfer of nanoparticles to the low-polarity phase. The promoter accelerates the phase separation, and also provides improved colloidal stability of the final substantially non-polar colloidal dispersion. Importantly, the glycol ether promoter reduces the temperature necessary to achieve the phase separation, while providing high extraction yield of nanoparticles into the low-polarity organic phase.

The invention relates to a method for extracting and processing lavender essential oil, belonging to the technical field of preparation of lavender essential oil. The method comprises the following steps: (1) pulverizing lavender flowers to 10-40 meshes, filling into an extraction tank of which the temperature is 35-55 DEG C, introducing supercritical CO2 gas into the extraction tank at the flow rate of 15-25 L/hour, and extracting under the pressure of 16-28 MPa for 1.0-3.0 hours; and (2) introducing the CO2 gas after extracting the lavender essential oil into a separation kettle 1 and a separation kettle 2 to carry out separation, and respectively collecting lavender wax lavender essential oil at the separation kettle 1 and the separation kettle 2, wherein the pressure and temperature of the separation kettle 1 are respectively 6-8 MPa and 30-50 DEG C, and the pressure and temperature of the separation kettle 2 are respectively 4-6 MPa and 30-40 DEG C.

The invention discloses a process for extracting and washing cyclohexane oxime. The process comprises the following steps of: extracting an extractant and aqueous solution of cyclohexane oxime in a weight part ratio of 1:1 to 2:1 at the temperature of between 45 and 55 DEG C; washing an organic phase obtained by the extraction with desalted water when the mass concentration of the cyclohexane oxime is less than 1 percent so as to remove salt ions in the cyclohexane oxime solution; mixing a water phase obtained after washing and a water phase obtained by extraction, and extracting the mixture by using a cyclohexane or hexane extractant in a weight part ratio of 0.5:1 at the temperature of between 45 and 55 DEG C so as to recycle the cyclohexane oxime in the mixture; and sequentially performing hydraulic cyclone separation and gravity settling separation on the washed organic phase when the mass concentration of the salt ions in the organic phase is less than 0.0001 percent so as to obtain the cyclohexane oxime. The process does not need a rectification process; the purity of the obtained cyclohexane oxime is high; beckmann rearrangement can be directly performed in the next process so as to prepare caprolactam; and for 100,000 t/a of cyclohexanone oxime devices, the process can save one-time equipment investment by about 20 to 30 million and can reduce energy consumption such as water, electricity, steam and the like by about 30 to 40 million.

The invention discloses a method for extracting theophylline sodium salt or theophylline from a theophylline sodium salt or theophylline mother solution by a styrene type macroporous resin adsorption method, which comprises the following steps: (1) regulating the pH value of the theophylline sodium salt or theophylline mother solution; (2) filtering the mother solution and removing suspended impurities in the mother solution; (3) adsorbing the mother solution by adsorption resin; (4) desorbing the adsorption resin after adsorption saturation by a desorbing agent to obtain a desorption solution; (5) regulating the pH value of the desorption solution, standing and crystallizing theophylline sodium salt or theophylline; (6) centrifugally filtering; and (7) drying to obtain solid theophylline sodium salt. The method provided by the invention has the advantages that the theophylline sodium salt or theophylline crude product has better quality, the operation safety is better, the extraction efficiency is higher, the extraction cost is low, and the method is more suitable for mass production and facilitates realizing automatic operation. With the method disclosed by the invention, the extraction cost of the theophylline sodium salt and theophylline can be reduced, the extraction yield of the theophylline sodium salt and theophylline is increased, and the competitiveness of enterprises is enhanced.

The invention relates to a preparation method for a novel red ginseng using reduced-pressure drying after high temperature high pressure process, also relates to the red ginseng prepared by the method, health functional food containing the red ginseng and red ginseng tea. Compared with common red ginseng, the novel red ginseng prepared by the method has the following features: contents of effective constituents like total phenol compound, maltol and ginsenoside are increased; color darkening phenomenon of the red ginseng is prevented, and the phenomenon is caused by a conventional method that high temperature high pressure process or repeated steaming and drying process are carried out to increase the effective constituent content; round ginseng state can be maintained; red ginseng with high extraction yield can be made in a short time; and the red ginseng can be smashed to make red ginseng tea powder, and the red ginseng tea powder can be used in warm water for fast releasing ingredients being beneficial to human body.

The invention discloses a method for separating and purifying huperzine by a molecular imprinting technology, which includes the following steps: 1 huperzia serrata is smashed and extracted in ultrasonic counter-current mode, obtained extracting solution is decompressed and concentrated, and then active carbon is added in the extracting solution to perform decoloration; 2 the decolorized extracting solution is arranged on a solid phase column, huperzine molecular imprinting polymer serving as a solid phase is installed in the solid phase column, 5% to 10% of ethanol water or methanol water is used for elution, 40% to 50% of ethanol water or methanol water is used for elution till eluent is colorless, an disassembling column enables the solid phase to be taken out from the solid phase column, and 60% to 70% of ethanol water or methanol water is used for extracting adsorbed huperzine monomers in the solid phase to obtain extraction liquid; and 3 the extraction liquid is decompressed, concentrated and recrystallized by using solvent to obtain the huperzine. The method for preparing the huperzine has the advantages of being safe, easy and simple to operate, low in cost, short in production cycle, high in purity and the like.

The invention discloses a light food composition for weight-loss meal replacement and a preparation method thereof. The light food composition for weight-loss meal replacement comprises 10-15 parts ofcereal powder, 8-14 parts of composite grease microcapsules,3-6 parts of fiveleaf gynostemma herb microcapsules, 6-13 parts of resistant dextrin, 6-13 parts of xylitol, 7-14 parts of soy isolate protein, 5-13 parts of medium-chain triglyceride powder, 5-10 parts of inulin, 2-5 parts of stachyose, 1-5 parts of chia seeds, 1-5 parts of konjaku flour, 0.7-3 parts of wheat oligopeptide, 0.2-2 parts of xylooligosaccharide powder, 0.1-2 parts of white kidney bean extract and 10-15 parts of composite fruit powder.