Method for extracting vitamine B2

An extraction method and vitamin technology, which is applied in the field of extracting vitamin B2 from vitamin B2 fermentation liquid, can solve the problems of large environmental pollution, high production cost, and large investment in equipment, and achieve the effects of low operating cost, high solubility, and simple method

Active Publication Date: 2005-10-26
ZHEJIANG QIANJIANG BIOCHEMICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This extraction method has a long process flow and high energy consumption. The purity of one-time dissolution, separation and crystallization is only 60% to 70%, and t

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Take 1000ml Vitamin B 2 Fermentation broth (titer 18212μg / ml), add 1000ml water to dilute, adjust pH value to 11.8 with 30% liquid caustic soda, stir for 30min; add calcium chloride 8g, disodium hydrogen phosphate 8g and 5% sodium polyacrylate solution 50ml to carry out Flocculation, and then filtration; the resulting filtrate was added with 2.5ml H 2 o 2 , adjust the pH to 4.2 with hydrochloric acid, and stir with air bubbling for 30 minutes; stand still for 2 hours, pour off half of the supernatant, heat and boil the remaining material for 15 minutes, cool to room temperature, filter the filter cake and wash it with pure water to obtain a wet crude product 18.5g; dissolve with 250ml of water, then adjust the pH to 11.8 with 30% liquid caustic soda, dissolve the material, filter; add H to the filtrate 2 o 2 2.5ml, adjust the pH to 4.2 with hydrochloric acid, stir with air bubbling for 30min; stand still for 2hr, heat and boil for 15min, cool to room temperature, fil...

Embodiment 2

[0029] Take 1000ml of fermentation broth (titer 17924μg / ml), add 1000ml of water to dilute, adjust the pH value to 11.8 with 30% liquid caustic soda, stir for 30min; add calcium chloride 8g, disodium hydrogen phosphate 8g and 5% sodium polyacrylate solution 50ml for flocculation, and then filtered; the resulting filtrate was added with 2.0ml H 2 o 2 , adjust the pH to 4.0 with hydrochloric acid and stir with air bubbling for 1 hr; stand still for 2 hrs, pour off half of the supernatant, heat and boil the remaining material for 15 min, cool to room temperature, and filter to obtain 17.5 g of wet crude product; dissolve in 250 ml of water , adjust the pH to 11.8 with 30% liquid caustic soda, dissolve the material, filter; add H to the filtrate 2 o 2 2.0ml and adjust the pH to 4.0 with hydrochloric acid, stir with air bubbling for 1hr; stand still for 2hr, heat and boil for 15min, cool to room temperature, filter to obtain the finished product; dry at 60°C, pulverize, pass thro...

Embodiment 3

[0031] Take 1000ml of fermentation broth (titer 18122μg / ml), add 1000ml of water to dilute, adjust the pH value to 11.8 with 30% liquid caustic soda, stir for 30min; add calcium chloride 8g, disodium hydrogen phosphate 8g and 5% sodium polyacrylate solution 50ml for flocculation, and then filtered; the resulting filtrate was added with 2.2ml H 2 o 2 , adjust the pH to 4.1 with hydrochloric acid and stir with air bubbling for 40 minutes; stand still for 2 hours, pour off half of the supernatant, heat and boil the remaining material for 15 minutes, cool to room temperature, and filter to obtain 17.7 g of wet crude product; dissolve in 230 ml of water , adjust the pH to 11.8 with 30% liquid caustic soda, dissolve the material, filter; add H to the filtrate 2 o 2 2.2ml and adjust the pH to 4.0 with hydrochloric acid, stir with air bubbling for 1hr; stand still for 2hr, heat and boil for 15min, cool to room temperature, filter to obtain the finished product; dry at 60°C, pulveriz...

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PUM

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Abstract

The present invention relates to a method for extracting vitamin B2 from vitamni B2 fermentation liquor. Said method utilizes addition of calcium chloride, disodium hydrogen phosphate and sodium polyacrylate to make flocculation, after filtration adds aqueous hydrogen peroxide solution to make precipitation and separation, then makes crystallization and recrystallization so as to obtain the invented vitamin B2 whose extracted yield is 85%-87%, and whose purity can be up to 98%-100%.

Description

technical field [0001] The invention belongs to a chemical extraction technology, in particular to a 2 B vitamins extracted from fermented broth 2 Methods. Background technique [0002] Vitamin B 2 It is an organic substance that is necessary for animal metabolism but cannot be synthesized by itself. Vitamin B 2 It is mainly used as a feed additive for livestock and poultry. It can improve feed conversion rate, promote animal growth, improve animal reproduction ability and ability to resist harsh environments, and has various health care functions and therapeutic effects such as anti-oxidation and anti-tumor. Both literature and textbooks talk about vitamin B 2 It is easy to be destroyed when encountering alkali, so vitamin B 2 Extraction studies have mostly focused on dissolving B vitamins with strong acids 2 The method; in the production, the acid-soluble method is also used to extract vitamin B 2 . The extraction method has a long process f...

Claims

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Application Information

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IPC IPC(8): C07D487/04
Inventor 裘国寅王雪钢
Owner ZHEJIANG QIANJIANG BIOCHEMICAL CO LTD
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