Isolation and purification method of amygdalin from loquat core

A technology for the separation and purification of amygdalin, which is applied in the direction of medical preparations containing active ingredients, organic chemistry, and pharmaceutical formulas, and can solve problems such as environmental pollution and waste of resources, achieve high recovery rates, reduce preparation costs, and penetrate powerful effect

Inactive Publication Date: 2007-09-19
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the annual output of loquat fresh fruit in the country has reached 160,000 to 200,000 tons, and tens of thousands of tons of loquat cores have been discarded without being used, which not only pollutes the environment, but also wastes precious resources.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Using loquat kernels produced in Putian, Fujian as raw materials, wash fresh loquat kernels with water, dry, crush, and pass through a 10-30 mesh sieve to obtain loquat kernel powder, put 3.0kg of loquat kernel powder into an extraction tank, and use 10 times the weight of loquat kernel powder (W / W) 48% methanol-acetone (1:1) aqueous solution, extracted 3 times at 63°C, 2 hours each time, combined the extracts, filtered, and the filtrate was filtered at -0.06~-0.09MPa, 50 Under the condition of ~65°C, the solvent is recovered under reduced pressure and concentrated in vacuum to obtain the crude extract of amygdalin.

[0029] Add 95% ethanol to the extract, so that the final ethanol concentration in the system is 81%, fully dissolve amygdalin at 80°C, filter, and filter the filtrate under reduced pressure at -0.06~-0.09MPa, 50~75°C Recover ethanol until amygdalin starts to precipitate, refrigerate at 4-10°C, let stand to grow crystals, and filter to obtain a beige-yellow...

Embodiment 2

[0032] Put 2.5 kg of 10-30 mesh loquat core powder into an extraction tank, extract 2 times at 50°C with 30% aqueous methanol solution of 20 times the weight (W / W) of the loquat core powder, and combine the extractions for 3 hours each time. liquid, filtered, and the filtrate was subjected to the conditions of -0.06~-0.09MPa, 50~65°C, the solvent was recovered under reduced pressure, and the amygdalin crude extract was concentrated in vacuum.

[0033]Add 95% ethanol to the extract to make the final concentration of ethanol in the system 88%, fully dissolve amygdalin at 75°C, filter, and recover the filtrate under reduced pressure at -0.06~-0.09MPa, 50~75°C Ethanol until amygdalin begins to precipitate, refrigerate at 4-10°C, let stand to grow crystals, and filter to obtain a beige-yellow amygdalin crude product.

[0034] Under the condition of 68°C, fully dissolve the crude product of amygdalin with 5 times the amount (W / W) of absolute ethanol, filter, and slowly cool the filt...

Embodiment 3

[0036] Put 2.5kg of 10-30 mesh loquat core powder into an extraction tank, use 65% aqueous methanol solution of 17 times the weight (W / W) of the loquat core powder, reflux extraction for 1 hour at 80°C, filter, and filter the filtrate through the filtrate. Under the conditions of -0.06~-0.09MPa and 50~65°C, the solvent is recovered under reduced pressure and vacuum concentrated to obtain the crude extract of amygdalin.

[0037] Add absolute ethanol to the extract to make the final concentration of ethanol in the system 90%, fully dissolve amygdalin at 60°C, filter, and recover the filtrate under reduced pressure at -0.06~-0.09MPa, 50~75°C Ethanol until amygdalin begins to precipitate, refrigerate at 4-10°C, let stand to grow crystals, and filter to obtain a beige-yellow amygdalin crude product.

[0038] Under the condition of 75°C, fully dissolve the crude product of amygdalin with 3 times the amount (W / W) of absolute ethanol, filter, and slowly cool the filtrate until the amy...

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Abstract

The invention provides a method for separating the purified amygdalin from the loquat nucleus, comprising: extracting the amygdalin from the loquat nucleus to produce a concrete with a hydrophilic organic water solution; fully dissolving the concrete by ethanol, filtering, depressed dealcoholization of the filtrate, cryogenic crystallization to produce the coarse amygdalin; fully dissolving the amygdalin, filtering, cryogenic recrystallization, washing by ethanol-aether, getting a amygdalin white crystal with a purity not less than 98.7%. The invention purifies the amygdalin from the loquat nucleus which solves the problem that the amygdalin as an original medicine is hydrolyzed by acid and enzyme so as to reduce the curative effect and has a broad extension.

Description

technical field [0001] The invention relates to a green chemical preparation method for extracting medicinal active ingredients from plant nuts, and more particularly relates to a method for separating and purifying amygdalin from loquat kernels. Background technique [0002] Amygdalin (Amygdalin, C 20 h 27 o 11 ) is an active ingredient of traditional Chinese medicine bitter almond, widely present in the kernels of various Rosaceae plants such as apricot, peach, plum, loquat, apple, hawthorn, etc., especially in bitter almond, with a content of about 2-3% . [0003] Amygdalin has obvious effects of relieving cough, relieving asthma, analgesia, anti-inflammation, anti-tumor, and promoting digestion. Traditional Chinese medicine often uses bitter almonds as medicine, but often due to the bitterness of the active ingredients during storage and boiling Amygdalin is easily hydrolyzed by acids and enzymes, and the obtained α-hydroxyphenylacetonitrile aglycone is very unstable...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/18A61K36/736
Inventor 陈剑锋陈浩郭养浩
Owner FUZHOU UNIV
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