Method for extracting phycocyanin from spirulina by using ammonia chloride solution

A technology of phycocyanin and ammonium chloride solution, which is applied in the field of natural product extraction, can solve the problems of long process route, high energy consumption, and low production efficiency, and achieve the effect of simple process and high extraction yield

Pending Publication Date: 2017-05-31
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problem of the above process is that the process route is long, most of the process needs to be processed at low temperature, the energy consumption is high, and the extraction cycle is long, resulting in low production efficiency, high cost, and low production and application value

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Spirulina cultivation and collection:

[0037] 1) Prepare the artificial seawater medium for Spirulina cultivation, the composition of the medium: 12.0g / L NaHCO 3 , 4.03g / L Na 2 CO 3 , 0.4g / L K 2HPO 4 , 2.5g / L NaNO 3 , 1.25g / L K 2 SO 4 , 1.25g / L NaCl, 0.25g / LMgSO 4 2H 2 O, 0.05g / L CaCl 2 2H 2 O, 0.01g / L FeSO 4 ·7H 2 O, 0.08g / L EDTA. Spirulina was inoculated and cultured in a 100L bubbling tube type photoreactor, the pH value of the medium was maintained at 9.0, the culture temperature was controlled at 30°C, and the concentration of 2.0% CO was continuously introduced. 2 , the light intensity of the LED light source is controlled at 100lux, the light and dark culture time are 12 hours and the culture time is 8 days alternately, and the density of Spirulina can reach 20g / L. Harvest the bacteria.

[0038] 2) The spirulina culture solution cultivated at high density is separated from the culture solution through the continuous separation tubular ceramic memb...

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Abstract

The invention relates to a method for extracting phycocyanin from spirulina by using an ammonia chloride solution, which belongs to the technical field of the extraction of natural products. The method comprises the following steps: (1) performing enzymolysis and wall breaking on the spirulina to obtain wall-broken spirulina; (2) mixing the wall-broken spirulina and the ammonia chloride solution, extracting the phycocyanin into the ammonia chloride solution, and performing ultra-filtration to obtain phycocyanin supernatant; and (3) salting out the phycocyanin supernatant by adopting an ammonium phosphate solution, and desalting a salted-out product, and drying to obtain the phycocyanin. By adopting the method, the low-temperature treatment is not needed, and the process is simple; and by virtue of the process for rapidly separating and extracting the phycocyanin by the ammonia chloride solution, the extraction yield is high, and the purity of the phycocyanin (A620 / A280) can reach 3.8 or higher.

Description

technical field [0001] The invention relates to the technical field of natural product extraction, in particular to a method for extracting phycocyanin from spirulina by using ammonium chloride solution. Background technique [0002] Phycocyanin (PC) is a photosynthetic pigment present in cyanobacteria cells, which can efficiently capture light energy. Because of its water-soluble, non-toxic, clear and strong coloring power, phycocyanin is widely used in food colorants and cosmetic additives. Phycocyanin is fluorescent and can be used as a fluorescent marker. Phycocyanin has obvious curative effect on anti-tumor and improving immunity. [0003] Among the existing methods involving the extraction and purification of phycocyanin, the separation and purification process of phycocyanin mainly includes the use of low temperature (-20°C to -10°C) repeated freezing and thawing methods or ice powder (-50°C to -10°C) to break the spirulina Add phosphate buffer to separate and extr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/795C07K1/36C07K1/34C07K1/30
CPCC07K14/795
Inventor 敬科举唐圆玮朱先锋郭立卢英华
Owner XIAMEN UNIV
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