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Film technological extracting method for L-phenylalanine

A technology of phenylalanine and extraction method, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of large amount, high consumption, low product purity and the like

Inactive Publication Date: 2003-06-25
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Activation, regeneration and cleaning of ion exchange method resin need to consume a large amount of acid and alkali, and required equipment and resin cost are higher simultaneously; Metal salt precipitation method all produces a large amount of salt and waste water, and the yield of extracting simultaneously (generally lower than 80%) ) and the purity of the product is relatively low; therefore it is very urgent to develop a high-yield, low-cost L-phenylalanine extraction method to promote the industrialization of L-phenylalanine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Take 10 liters of enzymatic conversion solution, the concentration of L-phenylalanine is 20 g / L, select 0.1m 2 The inorganic ceramic membrane is filtered at 0.1MPa and 25C, and the light transmittance of the filtered solution is 86%. The material of the inorganic membrane is alumina or zirconia, and the membrane pore size is 0.2-1μ. The filtrate utilizes a polysulfone membrane to remove miscellaneous proteins, the solution filtration pressure is 0.15-0.25Mpa, and the membrane molecular weight is 5000-30000 to obtain 11 liters of L-phenylalanine primary extract with a protein removal rate of more than 95%. The concentration of L-phenylalanine was 17.6 g / L, and the yield of L-phenylalanine was 96.7%.

Embodiment 2

[0019] Take 10 liters of enzymatic conversion solution, the concentration of L-phenylalanine is 20 g / L, filter with polysulfone membrane, the light transmittance of the solution reaches 82%, and the molecular weight of the membrane is 30000-100000. The filtrate utilizes a polysulfone membrane to remove miscellaneous proteins, the solution filtration pressure is 0.15-0.25MPa, and the membrane molecular weight is 5000-30000 to obtain 12 liters of L-phenylalanine primary extract with a protein removal rate of 94%. - The concentration of phenylalanine is 16.2 g / l and the yield of L-phenylalanine is 97.2%.

Embodiment 3

[0021] Get 10 liters of the first extract of Example 1 (the concentration of phenylalanine is 17.6 grams per liter), use polysulfone membrane as filter membrane, the molecular weight of membrane is 1000-3000, at 1.5-3.5MPa, 25-40 ℃ Under the condition of filtration and decolorization, the color removal rate is above 97%, and the yield of L-phenylalanine is 97.5%; the decolorization solution is desalted and concentrated by polysulfone membrane with a molecular weight of 200-800, and the removal rate of sodium chloride is When the solution was concentrated to 50-70 g / L, the pH was adjusted to 5.0-6.0 with sodium hydroxide, crystallized and precipitated to obtain 159.1 g of L-phenylalanine crystals with a purity of 96.7%.

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PUM

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Abstract

The present invention relates to the technological process of film extracting L-phynylalanine as medicine intermediate. To L-phynylalanine solution prepared with pyenyl-pyruvic acid through enzyme process, the film separation process, including eliminating thallus and cell fragment, ultrafiltration to elimiante hybrid protein, nanometer filtering to desalt and decolor and concentrate, to separate and extract target product. The said process has L-phynylalanine yield over 85%, and compared with available extraction process including ion exchange and metal salt precipitation, the said process has higher yield, and less consumption of acid, alkali, water and steam.

Description

technical field [0001] The invention relates to a membrane separation technology extraction method for pharmaceutical intermediate L-phenylalanine, which aims at preparing L-phenylalanine conversion solution by enzymatic method, and realizes high yield and low energy consumption of L-phenylalanine by using membrane separation technology. consumption separation and extraction. Background technique [0002] L-phenylalanine is one of the eight essential amino acids. The amino acid is mainly used: (1) as a food additive or additive synthetic raw material, such as one of the main raw materials of the new health-care sweetener aspartame is L-phenylalanine; (2) as a pharmaceutical raw material, such as Amino acid infusion, synthetic raw materials of amino acid anticancer drugs and antiviral drugs, etc. Among them, aspartame, as the most popular sweetener product in the international market in the 1990s, has been widely used in the food and beverage industries, and has become the ...

Claims

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Application Information

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IPC IPC(8): C07C227/40C07C229/36
Inventor 韦萍周华何若平万红贵欧阳平凯
Owner NANJING UNIV OF TECH
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