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A production method, glutamic acid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problem of increased consumption of sulfuric acid, ammonia water, glutamic acid impurities, poor quality, and environmental protection management costs Advanced problems, to achieve the effect of shortening the production cycle, ensuring product purity, and saving process water
Active Publication Date: 2012-10-03
HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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Problems solved by technology
The ubiquitous ion-exchange liquid is bulky, which increases the consumption of sulfuric acid and ammonia, and inevitably increases a large amount of waste water and raw materials.
Not only the cost of environmental protection treatment is high, but also the obtained glutamic acid impurities are of poor quality
Method used
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Embodiment 1
[0022] (1) First prepare glutamic acid fermentation broth: cultivate seeds, prepare seed medium (below are all mass percentages): glucose 2.5%, corn steep liquor 3%, K 2 HPO 4 ·3H 2 O 0.2%, Na 2 HPO 4 0.2%, biotin 0.5%, MgSO 4 ·7H 2 O 0.2%, urea 0.025%, sterilized at 115°C for 15 minutes; insert the strains into the seed culture medium, the inoculum size was 3%; cultivated in a 5L automatic control fermenter under suitable temperature, pH and dissolved oxygen conditions to In the logarithmic phase, the seed solution was obtained;
[0023] (2) Aerobic fermentation: the seed liquid prepared in step (1) was inoculated and fermented with 15% inoculation amount, and the temperature of the fermentation liquid was controlled by sequential temperature raising mode: 0~5h was 33°C, 5~10h was 34°C, 10h ~18h is 35°C, 18~26h is 36°C, 26~32h is 37°C; introduce appropriate air, adjust the appropriate stirring speed, and adopt the staged oxygen supply mode to control the dissolved oxyge...
Embodiment 2
[0033] Brevibacterium tianjin T613 was selected as the fermentation strain.
[0034] (1) First prepare glutamic acid fermentation broth: cultivate seeds, prepare seed medium (below are all mass percentages): glucose 2.5%, corn steep liquor 3%, K 2 HPO 4 ·3H 2 O 0.2%, Na 2 HPO 4 0.2%, biotin 0.5%, MgSO 4 ·7H 2 O 0.2%, urea 0.025%, sterilized at 115°C for 15 minutes; insert the strains into the seed culture medium, the inoculum size was 3%; cultivated in a 5L automatic control fermenter under suitable temperature, pH and dissolved oxygen conditions to In the logarithmic phase, the seed solution was obtained;
[0035] (2) Aerobic fermentation: the seed liquid prepared in step (1) was inoculated and fermented with 15% inoculation amount, and the temperature of the fermentation liquid was controlled by sequential temperature raising mode: 0~5h was 33°C, 5~10h was 34°C, 10h ~18h is 35°C, 18~26h is 36°C, 26~32h is 37°C; introduce appropriate air, adjust the appropriate stirri...
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Abstract
The invention discloses a novel production method for glutamic acid, belonging to the technical field of the production of amino acid. The novel production method for the glutamic acid comprises the following steps of: removing thalli and insolubles by means of high-speed disc separation; evaporating and concentrating separated glutamic acid material liquid through a multi-effect plate type evaporator at low temperature, wherein the generated secondary steam condensed water is used for fermentation ingredients of the glutamic acid; performing continuous isoelectric extraction on the glutamic acid in the evaporated glutamic acid concentrated solution; absorbing the glutamic acid by making supernatant fluid pass through ion exchange columns; performing isoelectric reextraction on the analyzed glutamic acid; inputting high-concentration wastewater into a fertilizer workshop for producing fertilizer; squeezing heavy phase (mycoprotein) through a plate frame, and granulating; and drying through a fluid bed, and thus producing high-protein feed. The novel production method for the glutamic acid has the advantages of low unit consumption of liquid ammonia and sulfuric acid, high extraction yield of the glutamic acid, less ion exchange investment and the like; and meanwhile, the purity of the extracted glutamic acid is high, sodium glutamate can be produced without crystalloblast, resources are fully used in the whole process, the aims of energy conservation and consumption reduction are achieved, and the novel production method for the glutamic acid has a wide application prospect.
Description
technical field [0001] The invention relates to a method for efficiently producing glutamic acid, which belongs to the technical field of amino acid production by fermentation. Background technique [0002] Glutamic acid is the precursor of monosodium glutamate, which is mainly used in the production of monosodium glutamate. The most widely used method for the production of glutamic acid at home and abroad is the fermentation method. There are many techniques for extracting glutamic acid from the fermentation broth. The main methods are: : Glutamic acid extracted by isoelectric method, glutamic acid extracted by ion exchange method, glutamic acid extracted by zinc salt method, glutamic acid extracted by hydrochloride method or glutamic acid extracted by electrodialysis method, etc. [0003] Wherein the isoelectric method extracts glutamic acid and utilizes the following properties of glutamic acid: glutamic acid is a kind of ampholyte, and when the isoelectric point pH value...
Claims
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Application Information
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