Method for extracting beta-thymidine from fermentation liquid

A fermented liquid and thymidine technology, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of relatively large safety and environmental protection hazards, complex chemical synthesis process, and less thymidine extraction process , to achieve a high degree of automation, improve the extraction yield, and ensure the quality of the effect
CN105859809AInactive Publication Date: 2016-08-17NANCHANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANCHANG UNIV
Publication Date
2016-08-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for extracting beta-thymidine from fermentation liquid. The method comprises the following steps: (1) preheating the fermentation liquid; (2) performing microfiltration on the fermentation liquid; (3) performing ultrafiltration on the microfiltration liquid; (4) performing electrodialysis on the ultrafiltration liquid; (5) concentrating the electrodialysis liquid; (6) performing chromatography on the concentrated liquid; and (7) concentrating and crystallizing the chromatography liquid. According to the method, a membrane separation technique, an electrodialysis technique and a chromatography separation technique are implemented in the extraction process of the beta-thymidine fermentation liquid, so that a good impurity removal effect can be achieved, and the process is simple, convenient and environment-friendly and high in automation degree. By adopting the extraction process disclosed by the invention, the purity of the liquid phase of a beta-thymidine product is greater than or equal to 98.5%, the external standard content is greater than or equal to 98.5, the total yield is greater than or equal to 70%, industrial application of extracting beta-thymidine from the fermentation liquid is achieved, and good economic benefits and industrial popularization prospects can be achieved.
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Description

technical field

[0001] The invention relates to a method for extracting β-thymidine from fermentation liquid, belonging to the technical field of biochemical industry. Background technique

[0002] β-thymidine, namely 2'-deoxythymidine, is an important precursor for the synthesis of anti-AIDS drugs stavudine, azidothymidine and zidovudine. As the number of AIDS patients in the world increases year by year, the demand for thymidine, the raw material of synthetic anti-AIDS drugs, also increases year by year.

[0003] Currently, there are two main methods for producing β-thymidine. One is the production of thymidine by chemical synthesis. Due to the complexity of the chemical synthesis process, a large amount of organic solvents are needed in the synthesis process, and there are great safety and environmental risks in the production process; the other is the production of thymidine by biological fermentation, but the related thymidine There are still few research reports on t...

Claims

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