180 results about "Aureobasidium pullulans" patented technology

Three choline utilizing strains of microorganisms isolated from the anthers of wheat, Aureobasidium pullulans strain AS 55.2, Arthrobacter species strain OH 221.3, and Pseudomonas species strain AS 64.4, are superior antagonists of F. graminearum. These microorganisms are effective for suppression and control of FHB in cereals, particularly in wheat and barley.

The invention provides mutant aureobasidium pullulans TKPM00006 for massively producing beta-poly(malic acid) and a method for producing poly(malic acid) by using the mutant through fermentation. The strain is the mutant for producing beta-poly(malic acid) with high yield, which is cultured by multiple groups of composite mutagenesis technology based on beta-poly(malic acid) producing TKPM10017 screened in the soil of Wang Taibao orchard, Yinchuan, Ningxia Hui Autonomous Region, China. Under the optimization condition, the yield of beta-poly(malic acid) reaches 18.4+/-1.3g/l.

The invention relates to a fire-retardant polyethylene cable material with mould-proof function, which is characterized by comprising the following components by weight portion: 45 to 70 portions of high density polyethylene resin, 30 to 55 portions of linear low density polyethylene resin, 2.35 to 2.85 portions of carbon black, 35 to 65 portions of fire retardant, 3 to 6 portions of lubricant, 0.5 to 2 portions of antioxidant, and 1 to 2 portions of antibacterial agent. The method for preparing the fire-retardant polyethylene cable material with the mould-proof function comprises the following steps: weighing raw materials according to a formulation, mixing the materials, and performing the processes of extruding, granulating and drying the materials to prepare the mould-proof fire-retardant polyethylene cable material. The mould-proof fire-retardant polyethylene cable material can effectively inhibit the growth, the forming and the reproduction of eight types of mould fungi including aspergillus niger, aspergillus terreus, aureobasidium pullulans which can easily grow on a cable under the condition that the original performances and the cabling process of the fire-retardant polyethylene cable material are not influenced, thus the cable is prevented from being infringed by the mould fungi, and a number of unnecessary loss and hidden hazards to the society are avoided.

The present invention relates to utilizing saccharide metabolism to produce extracellular water soluble polysaccharide and belongs to the field of bioengineering technology. The present invention provides strain, named Aureobasidium pullulans G-58 and with the preservation number of CGMCC No. 1234. The pullulan producing process with the strain includes fermentation culture, fermented liquid post-treatment, heat treatment, decolorizing, precipitation, desalting, drying and other steps. The pullulan product has light color, high polysaccharide content, excellent viscoelasticity, excellent emulsifying performance and plasticity, and no toxicity and harm, and may be used widely in food, medicine, cosmetics, etc.

The invention discloses a bacterial strain for producing PMLA [Poly (Beta-L-malic acid)], which is obtained by screening the surface of a plant leaf and can not produce melanin. The classification name is Aureobasidium pullulans, the Latin literature name is Aureobasisdium pullulans ZD-3D, and the preservation number of the bacterial strain is CGMCC No.4605. The method for producing PMLA by the bacterial strain is simple to implement and is beneficial to the separation and purification of PMLA, and the yield can be up to 50-60g/L.

The invention relates to a comprehensive application of salicornia bigelovii. The salicornia bigelovii comprises fresh vegetables and tender stems of plants; active substances are extracted after the salicornia bigelovii is pulped or dried and crushed; the active substances comprise components such as polysaccharide, ferulic acid, general flavone and the like; the residues of salicornia bigelovii after the active substances are extracted are inoculated with food-security microorganism aureobasidium pullulans for fermentation so as to produce fermented products rich in single-cell protein (SCP) and viscous pullulan which has been allowed by the FDA (food and drug administration) in Japan to be used as a food additive, the pullulan produced by self fermentation is utilized as a binding agent, infrared and microwave technologies are adopted to dewater, dry and form fermentation mud, and the fermented products are prepared into instant vegetable paper with rich nutrition, so that the variety of leisure foods is richened and the high-additional-value comprehensive utilization system of the salicornia bigelovii is constructed.

The invention discloses a method for producing poly(malic acid) and pullulan together by using Aureobasidium pullulans. The invention relates to a method for producing poly(malic acid) and pullulan by using Aureobasidium pullulans, belonging to the technical field of the bio-fermentation engineering. The method comprises the following steps: reducing the temperature of the culture medium of a fermentation tank to 28 DEG C, inoculating a strain in the fermentation tank according to the inoculation quantity which is 3%, stirring under the conditions that the rotational speed is 200rpm and the ventilation rate is 0.5VVM, controlling the temperature to 28-31 DEG C to begin fermentation, performing the fed-batch of sucrose after 24 hours to control the content of sucrose in the fermentation liquor to 35g/L, controlling the temperature to about 25 DEG C after 72 hours, continuously performing the fed-batch of sucrose to control the content of sucrose in the fermentation liquor to 15 g/L, fermenting for 136 hours to stop adding sucrose, continuously fermenting for 8 hours when the content of sucrose in the fermentation liquor is reduced to 0, controlling the pH value to 5-7.2 in the fermentation process, and fermenting for 166 hours. By adopting the method disclosed by the invention, the raw material utilization rate can be increased, energy can be saved and the cost can be reduced.

The invention discloses a method for increasing pulullan yield by sub-sectional adding of growth factors in a fermentation process, and belongs to the technical field of biological fermentation engineering. The method comprises the following steps: producing pulullan from aureobasidium pullulans CGMCC NO.7055 in a fermentation manner; adding 0.003-0.005% of vitamin B1 when bacteria growth is at the beginning of the logarithmic phase; and adding 0.002-0.004% of uridine monophosphate when the bacteria growth is at the early stage of a stable stage, so as to induce and facilitate accumulation of secondary metabolite, namely the pulullan. The growth factors are specifically added according to the characteristics of sugar production in different growth periods and fermentation processes, so that the method is convenient and fast to operate and has an obvious effect; the fermentation period is greatly shortened, the transformation rate of a substrate is increased, and the cost of the pulullan is reduced.

The invention discloses a method for asymmetrically reducing a carbonyl compound by biocatalysis in a water/ion liquid biphasic system, which belongs to the biochemical engineering technical field. The method takes a selective strain producing carbonyl reductase as a starting strain, and prochiral ketone as a substrate to perform reducing preparation of corresponding chiral alcohol in the water/ion liquid biphasic system; the method comprises the following steps that: Aureobasidium pullulans CGMCC No.1244 is used to catalyze 4-chloro-acetoacetic acid ethyl ester to perform the asymmetrical reduction preparation of (S)-4-chloro-3-hydroxybutyric acid ethyl ester, and Saccharoinyces uvarum ATCC 26602 is used to catalyze 4,4,4-trifluoroacetoacetate to perform the asymmetrical reduction preparation of (R)-4, 4, 4-trilfluoro-3-hydroxybutyrate. The method shows the advantages of no toxicity, no smell, difficult volatilization, good biocompatibility, no environmental pollution, simple product separation, easy recovery, repeated use and so on, of ion liquid. At the same time, the method improves the transformation rate, the concentration and an enantiomeric excess value of a reaction product, and quickens the process of the reaction.

The invention discloses a liquid plastic film, and belongs to the technical field of agriculture. The liquid plastic film consists of the following components in percentage by weight: 1%-10% of pullulan polysaccharide, 1%-10% of polyglutamic acid and the balance of water, wherein the pullulan polysaccharide is prepared by fermenting aureobasidium pullulans of which the preservation No. is CGMCC No.7055, and the polyglutamic acid is prepared by fermenting bacillus licheniformis. The invention further discloses a preparation method and a use method of the liquid plastic film. The liquid plastic film is biodegradable and does not pollute environment, and after the plastic film is sprayed onto the surface of soil, the temperature of the soil can be raised, the evaporation of water is restrained, water content of the soil is increased, the salt content of the soil is reduced, and the soil can also be improved, so that the physicochemical property of the soil is better, and the growth of plants is promoted; besides, the liquid plastic film has the advantages of simple preparation method, low cost, good convenience in use, saving of labors and material, low cost and easiness in obtaining of raw materials, simple production equipment and technology, zero pollution in a production process, and the like.

The invention relates to a fermentation production method of pullulan without pigment. Aureobasidium pullulans, which are used as the starting strain, are induced by microwaves to obtain the mutant strain with high-yield pullulan and little pigment secretion. The fermentation production method of the pullulan comprises the following steps: (1) preparing seeds and fermentation medium; (2) inoculating the activated strain to the seed culture medium according to the inoculum size of 2-10%, and culturing at 28+/-1 DEG C for 24-36 hours, wherein the concentration of seeds is 0.8-2.5*108/mL; (3) inoculating the seed liquid to the fermentation medium according to the inoculum size of 2-10%, pressurizing to 0.01-0.05 Mpa in a tank at 28+/-1 DEG C, adjusting the stirring rate and ventilation amount at different fermentation stages and fermenting for 60-72 hours; and (4) after-treatment of fermentation liquid: using decoloration of the fermentation liquid, plate-and-frame filter, ceramic membrane ultrafiltration ultrafiltering for the sugar solution and the other methods to carrying out ultrafiltration and concentratuion, and performing spray drying to obtain the pullulan product.

The invention discloses a collagen skin firming lifting plumping glowing cream which comprises red ginseng snail secreta filter liquor, soluble collagen, hydrolysis collagen, atelocollagen, sodium hyaluronate, caesalpinia spinosa fruit extracts/Kappaphycus alvarezii extracts, nicotinamide, mica, hydrogenated phosphatidylcholine, lysoph-osphatidylcholine/sclerotium gum/xanthan gum/aureobasidium pullulans polysaccharide, mentha piperita extracts, hydroxyethyl acrylate/acryloyl dimethyl sodium taurocholate copolymers. Periocular skins are firmed, lifted and glowed from outside to inside by the aid of the cream. Ray refraction modes are adjusted by the aid of the mica, so that wrinkles are flatted, skins are brightened, melanin can be restrained by the nicotinamide, black eyes are faded, 'external regulation and internal nourishing' are achieved, the periocular skins are rapidly and effectively brightened and rapidly firmed and lifted, the hydrogenated phosphatidylcholine serves as emulsifying agents and thickening agents, mild and non-irritating effect is achieved, the mentha piperita extracts bring cool and refreshing skin feeling, fatigue is relieved, generation of fat granules is prevented, and the healthy state of the skins is kept.

The invention relates to the field of microorganisms and discloses aureobasidium pullulans.The preservation number of the aureobasidium pullulans is CGMCC No.11937.The aureobasidium pullulans with the preservation number being CGMCC No.11937 uses wild aureobasidium pullulans with the preservation number being CGMCC No.3.4580 as an original strain, chemical induced mutation is performed, the aureobasidium pullulans is obtained through screening, the capacity that the aureobasidium pullulans ferments to generate pullulans is remarkably improved, compared with the original strain, the yield of the pullulans is increased by 80%, and the glucose conversion rate is increased by 68%.The color of the finished pullulans becomes lighter obviously.Thus, the aureobasidium pullulans with the preservation number being CGMCC No.11937 can be widely applied to pullulans fermentation.

The invention discloses a collagen nano instant mask taking aureobasidium pullulans polysaccharide as a framework and a preparation method. The mask comprises a non-woven fabric base material layer and a nano-fiber layer, wherein the nano-fiber layer is formed on the non-woven fabric base material layer by adopting an electrostatic spinning method; the nano-fiber layer is prepared from the following components in parts by weight: 10 to 60 parts of the aureobasidium pullulans polysaccharide, 5 to 40 parts of hydrolyzed collagen with relatively low molecular weight and 0.1 to 5 parts of a surface active substance; the average molecular weight of the aureobasidium pullulans polysaccharide is 100000 to 300000; the average molecular weight of the hydrolyzed collagen with relatively low molecular weight is 1000 to 3000; the mass ratio of the aureobasidium pullulans polysaccharide to the hydrolyzed collagen with relatively low molecular weight ranges from (1 to 5) to (6 to 1).

The invention discloses a skin-care composition. The skin-care composition comprises a solvent, substrate solution, a thickening agent, an emulsifying agent, a pH modifier, a preservative and the following conditioning components: glycine betaine, mycose, aureobasidium pullulans polysaccharide, ROSA MULTIFLORA fruit extractive, ROAS RUGOSA oil, SCLEROTIUM ROLFSSII glue, sodium hyaluronate and hydrolyzed collagen. The skin-care composition can form a layer of moisturizing anti-cracking protection barrier with good weather resistance, and effectively remove free radical on the surface and surface layer of skin, and has the effects of oxidation resistance, skin whitening and aging resistance; the skin-care composition can effectively supplement skin collagen, and give skin elasticity and gloss; the skin-care composition cooperatively preserves moisture, keeps skin white and resists skin aging. A preparation method of the skin-care composition is beneficial to the obtaining of the skin-care composition uniform in preparation and capable of being properly absorbed by skin.

The invention discloses a nitrogen, phosphorus and potassium microbial coated fertilizer and a preparation method thereof. The nitrogen, phosphorus and potassium microbial coated fertilizer comprises a pill core, a middle layer and a mycoderm layer from inside to outside in sequence; the pill core comprises the following raw materials: urea, monopotassium phosphate, diammonium phosphate, calcium superphosphate, potassium sulphate, plant ash and glutinous rice glue; the middle layer comprises the following raw materials: furfural residues, vinasse, sorghum residues, edible fungus culture medium wastes, aureobasidium pullulans, EM (effective microorganism) bacterial liquid and clay; the mycoderm layer comprises the following raw materials: peat soil, a microbial agent, Carbomer, 10 wt% sodium hydroxide aqueous solution and water. The nitrogen, phosphorus and potassium microbial coated fertilizer disclosed by the invention has a viable count of more than 0.062 billion CFU/g, an organic content of more than 60.5 wt%, a pH value of 7.1-7.2 and an N, P and K content ratio of (2-3.5):(1.5-2.1):1; the reasonable collocation is performed according to nitrogen, phosphorus, potassium and other demands of crops, so that the release period of fertilizer nutrients can be prolonged; the microbial agent improves the soil texture and the tillage performance and inhibits the emergence of weeds and pests. The preparation method of the fertilizer has the advantages that the raw materials are easy to obtain, the operation is simple, the biological survival rate is high, the activity is good, and the use effect is outstanding.

The invention discloses a method for producing novel natural melanin. The method is characterized by comprising the following steps of: activating aureobasidium pullulans for 4 to 8 hours, performing shaking culture for 12 to 36 hours to obtain seed liquid, inoculating the seed liquid in a volume of which is 1 to 10 percent of that of a fermentation medium to the fermentation medium, and performing liquid state fermentation; centrifuging the fermentation solution obtained by fermentation for 5 minutes under the condition of 5,000r/min, performing acidolysis on the supernate by using hydrochloric acid, and then performing precipitation by using methanol to obtain a crude melanin product; dissolving the crude product to obtain a black solution by using an alkali solution; and performing precipitation on the obtained black solution by using hydrochloric acid, and thus obtaining the melanin. Compared with other fermentation medium, the method using the fermentation medium has the advantages that: the yield of the melanin is improved from 2.47g/L to 10.74g/L, the fermentation period is shortened from 7 days to 3 days, the culture substrate is fully utilized, the utilization rate of raw materials is improved, and the production cost is reduced.

The invention belongs to the technical field of microbes and in particular relates to an aureobasidium pullulans stain NCPS 2016-M capable of producing pulullan at high yield. The aureobasidium pullulans stain NCPS 2016-M capable of producing pulullan at high yield provided by the invention is characterized in that the strain is collected in China Center for Type Culture Collection Center on September 14, 2017, and has a collection number of CCTCC M 2017498. The aureobasidium pullulans stain disclosed by the invention has the beneficial effects that the amount of the pulullan produced by performing shaking flask fermentation on the aureobasidium pullulans stain provided by the invention reaches 60g/L, and the ability of producing the pullulans is 1.5-1.7 times that of a wild aureobasidiumpullulans stain. The mutant strain does not produce any melanin, and the fermentation time is shortened by six hours compared with that of an original strain. The technical problem that wide application of the pullulans in the domestic food industry, particularly in an agricultural product preservation aspect, is greatly limited because the pullulans production level is low and the product qualityis low is solved.

The invention relates to an Aureobasidium pullulans strain and an application thereof, and belongs to the technical fields of fermentation engineering and biology. The preservation name of the Aureobasidium pullulans strain is Aureobasidium pullulans ZK-502, the strain is preserved in CGMCC (China General Microbiological Culture Collection Center) on August 20, 2018 at No.3 of No.1 West Beichen Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 16326. The Aureobasidium pullulans strain can efficiently produce liamocin by fermentation and produces no melanin, fermentation broth is light pink to faint yellow, and follow-up treatment process can be simplified. The method is simple and feasible, raw materials are simple in composition, yield of liamocin can reach 8-10 g/L, and commercial and large-scale production of liamocin is expected to be realized; the obtained liamocin product is dissoluble in water and has wide application prospect in medicine, cosmetics andfood.

The invention discloses a microbial preparation for tobaccos and a preparation method and application thereof, belonging to the technical field of tobacco additives. According to the preparation method, the corresponding microbial preparation is obtained through carrying out liquid fermentation culture on producing strains, wherein the producing strains are aureobasidium pullulans OF-01 strains (CGMCC (China General Microbiological Culture Collection Center) No.7609). The microbial preparation disclosed by the invention has the advantages that the characters, such as aroma amount, pleasantness and the like, of cut stems can be improved obviously, offensive odors, irritation and the like are reduced, the concentration and momentum of smoke are reduced, and the aftertaste becomes comfortable, so that the blended amount of the cut stems in cigarettes is further increased, and the aims of reducing harm, lowering tar content and reducing the cigarette production cost are achieved.

The invention discloses a method for increasing the yield of pullulan. The method comprises the following steps: after activating aureobasidium pullulans, inoculating a seed medium; after OD600 is greater than 0.3, inoculating seeds into a fermentation medium; monitoring OD600 of a fermentation liquid in real time; when OD600 is greater than 0.2 and less than 0.3, adding a compounding agent I which accounts for 0.02-0.03% of the volume of the fermentation liquid, so as to greatly increase the bacterium growth velocity and remarkably shorten the logarithmic growth phase; when OD600 is greater than or equal to 0.6, adding a compounding agent II which accounts for 0.03-0.05% of the volume of the fermentation liquid. The substrate conversion speed can be accelerated, the polysaccharide synthesis performance can be improved, and the yield can be greatly increased.

The invention relates to a method for increasing pulullan output through fed-batch fermentation, which comprises the following steps: culturing seeds, performing fermentation culture and separating pulullan; continuously feeding a fed-batch solution after fermenting for 24-28 hours, controlling the residual sugar of a broth to maintain at 6-12g/L, supplementing 0.5-2g/L of amino acid solution for once by every two hours, wherein the total fermentation period is 65-67 hours. Aiming at defects of long period and low substrate conversion rate during a process of fermentation of aureobasidium pullulans with a preservation number of No. 7055 to produce pulullan, a proper amount of amino acid is continuously fed during the fermentation process for synthesis of pullulan, substrate conversion rate is increased, operation is convenient, effect is obvious, fermentation period is greatly shortened, and cost of pullulan can be reduced.