26 results about "Aureobasidium pullulans" patented technology

The invention relates to aureobasidium pullulans Cy28 and application thereof, and belongs to the technical field of microorganisms, the aureobasidium pullulans Cy28 can reduce the content of Cd in soil and can generate more pullulan in a Cd-containing environment; when the culture medium is applied to lettuce planting, the dry weight of edible tissues of the lettuce can be remarkably increased in a cadmium-free or cadmium-containing environment, and the content of Cd in the edible tissues and roots of the lettuce is reduced, so that the content of Cd in the edible tissues of the lettuce reaches the edible standard; in addition, the vitamin C content, the chlorophyll content, the soluble protein content and the IAA content of edible tissues of the lettuce can be improved; meanwhile, the content of beneficial microorganism Pseudarthrobacter in the soil can be remarkably increased in a cadmium environment, and the effect of improving the soil is achieved.

The present invention relates to the use of a cosmetic active ingredient comprising an extract of the biomass of at least one yeast of the species Aureobasidium pullulans, characterized in that the extract contains at least 25% by weight of sugars based on the dry matter of the extract, as a cosmetic active ingredient in a use or composition comprising the same.

Disclosed herein are methods of producing Massoia lactone from liamocins, or mixtures of liamocins and exophilins. Liamocins utilizable in the methodologies provided herein can be produced by microbes, such as Aureobasidium pullulans. The methodologies described herein utilize acid catalyzed hydrolysis / dehydration and carboxylic acids as a phase transfer agent in a dual-solvent system. This approach further allows the separation and collection of liamocin head groups.

The invention belongs to the technical field of gene engineering, and particularly relates to aureobasidium pullulans with high yield of pullulan and application of the aureobasidium pullulans. On the basis of aureobasidium pullulans M233-20, an isopullulanase gene A9187 gene in a genome of the aureobasidium pullulans M233-20 is knocked out based on a homologous recombination technology, and through polysaccharide fermentation test detection, an isopullulanase gene deletion mutant strain aureobasidium pullulans delta A9187-1 with the pullulan polysaccharide molecular weight improved and the yield not reduced is obtained. The weight-average molecular weight of the pullulan produced by the recombinant strain is 6.5 * 10 < 5 > Da, which is 2.6 times that of the original wild strain (2.46 * 10 < 5 >), and the yield of the pullulan is increased from 147.33 g / L to 156 g / L, which is increased by 5.88%.

The invention provides a compound preparation for inhibiting aspergillus flavus and application of the compound preparation in prevention and treatment of aspergillus flavus pollution, and the compound preparation comprises a compound microbial agent and a compound extract preparation, the compound microbial agent is prepared by mixing the following microbial agents in parts by weight: 5-10 parts of bacillus sorola, 5-10 parts of bacillus amyloliquefaciens and 3-8 parts of black yeast; the composite extract preparation is prepared by mixing a winged bean extract and a vanilla bean extract according to a weight ratio of (30-55): 1. The plant extract and the microbial agent are compounded to inhibit the aspergillus flavus, the antibacterial effect is good, and a new direction is provided for preventing and treating the aspergillus flavus of grain and oil crops and grain products.

The present disclosure provides an Aureobasidium pullulans CJFSKY2201 strain deposited under accession number KCCM13230P; a culture of the strain; use of the strain in producing oligosaccharides; a composition comprising the strain, a lysate thereof, a culture thereof, a concentrate thereof, or a dry product thereof; and a method of producing oligosaccharides, the method comprising the step of culturing the strain and the step of obtaining oligosaccharides from the culture.

The invention discloses a compound microbial agent rich in beta-glucan as well as a preparation method and application of the compound microbial agent, and belongs to the technical field of agricultural biology. According to the microbial agent, an aureobasidium pullulans-rhizobium pullulans-bacillus subtilis-bacillus thuringiensis quaternary synergistic system is constructed, specific auxiliary materials, carriers and a coating technology are supplemented, and under the drought stress condition, the microbial agent can remarkably promote the growth (plant height and biomass) of the pakchoi, so that the yield of the pakchoi is increased, and the yield of the pakchoi is increased. The yield of the pakchoi is increased, the quality is improved (the content of soluble sugar and protein is increased), the chlorophyll content and the photosynthetic efficiency are improved, the antioxidant enzyme activity is enhanced, cell membrane damage is reduced, the physical and chemical properties of soil are improved, and a new effective solution is provided for agricultural green drought resistance.

The application discloses application of melanin-producing Aureobasidium pullulans CNBG-PGPF-1 in improving saline soil and / or plant salt tolerance and / or promoting plant growth and belongs to the technical field of microorganisms.The strain CNBG-PGPF-1 has a promoting effect on seed germination and seedling growth of Medicago polymorpha under the condition of saline soil.The strain CNBG-PGPF-1 also enhances the salt stress tolerance of Medicago polymorpha by reducing the content of sodium ions, increasing the content of potassium ions, reducing the accumulation of hydrogen peroxide and increasing the content of proline.The melanin-producing Aureobasidium pullulans and the microbial agent thereof have a good application prospect in the improvement of saline soil and the production of crops in saline soil, and provide resource and technical support for further development of microbial agents or microbial manure suitable for the improvement of saline soil and the agricultural production in saline soil.

The present application provides a mutant glucanase, which has a mutation at the amino acid position corresponding to position 121 of SEQ ID No. 2 compared to the parent glucanase. The enzyme activity of the mutant glucanase of the present application is increased by 68% compared to the wild-type enzyme, and has a wide application prospect in degrading materials containing glucan (for example, Aureobasidium pullulans residues produced in the production of pullulan).

The application discloses a method for regulating high-efficiency expression of heterologous genes of Aureobasidium melanogenum based on rDNA integration vectors and application, first constructs integration vectors pAMrDNA1, pAMrDNA2 and pAMrDNA3 targeting three different regions of rDNA, compares integration efficiency by taking gfp as a report gene, and selects the optimal site rDNA1.On this basis, endogenous pks genes of Aureobasidium melanogenum are first knocked out, then pks gene back complementation integration is mediated by the pAMrDNA1 vector, stable multi-copy expression is realized, and further 11.0 kb melanin biosynthesis gene clusters are integrated into a three-gene knockout chassis strain by the pAMrDNA1 vector in one step, the highest 15.0 copy integration is realized, and melanin yield is increased by about 12 times.The application realizes stable and high-efficiency expression of heterologous genes of Aureobasidium melanogenum, and especially provides an effective tool for one-step integration of large fragment gene clusters.

Disclosed herein are methods of producing massoia lactone from liamocins, or mixtures of liamocins and exophilins. Liamocins utilizable in the methodologies provided herein can be produced by microbes, such as Aureobasidium pullulans. The methodologies described herein utilize acid catalyzed hydrolysis / dehydration and carboxylic acids as a phase transfer agent in a dual-solvent system. This approach further allows the separation and collection of liamocin head groups.

The invention relates to an aureobasidium pullulans engineering bacterium for producing low-molecular-weight pullulan and application of the aureobasidium pullulans engineering bacterium, and belongs to the technical field of biological engineering. According to the aureobasidium pullulans engineering bacterium disclosed by the invention, the alpha-amylase AMY gene, the glucamylase GLY gene and the pullulanase IPU gene are continuously overexpressed, so that the molecular weight of pullulan produced by fermenting the aureobasidium pullulans is remarkably reduced, and the fermentation titer of the strain is improved; the problem that the molecular weight of pullulan produced through fermentation of aureobasidium pullulans in the prior art is uncontrollable is solved, an excellent strain is provided for preparing pullulan through a microbial fermentation method, the separation cost in the downstream purification process of pullulan is reduced, an excellent strain is provided for industrial production of low-molecular-weight pullulan, and the method is suitable for industrial production of pullulan. The molecular weight of pullulan produced after the strain is fermented for 3 days is 9.2 * 10 < 3 > Da and is greatly reduced compared with the molecular weight 5.08 * 10 < 5 > Da of pullulan produced by a host strain, the molecular weight can be reduced to 3.6 * 10 < 3 > Da by optimizing a fermentation culture medium and culture conditions, and the pullulan can be widely applied to the fields of food additives, preparation of cosmetic raw materials and the like.

The invention discloses an aureobasidium sp. Strain ZX033 for producing fumaric acid and gluconic acid and application of the aureobasidium sp. Strain ZX033. The preservation number of the aureobasidium sp. Strain ZX033 is CCTCC M 20252559. The invention also provides a separation and identification method of the strain. The ITS sequence and the LSU sequence of the strain are respectively shown as SEQ ID NO.1 and SEQ ID NO.2. Experiments prove that the strain can simultaneously produce fumaric acid and gluconic acid through fermentation. Furthermore, a glucose oxidase (GOX) gene of the ZX033 strain is successfully knocked out through a genetic engineering means, and an engineering strain ZX033-delta GOX is constructed. The fumaric acid production capacity of the engineering strain is remarkably improved, compared with an original strain, the yield in a specific culture medium can be improved by 28.7% or above, and the engineering strain has better industrial application potential. The invention provides a new strain resource and a fermentation process for efficiently producing fumaric acid and gluconic acid.

The present application relates to the application of extracellular beta-glucan from Aureobasidium pullulans in preparing training immune inducer. By establishing mouse bone marrow derived macrophage (BMDMs) and zebrafish larvae training immune activation evaluation model, the immune activity of extracellular beta-glucan (eBG) from Aureobasidium pullulans is evaluated. It is found that compared with cell wall derived beta-glucan (cBG), the extracellular beta-glucan (eBG) from Aureobasidium pullulans has more significant training immune activation effect, which proves that the extracellular beta-glucan (eBG) from Aureobasidium pullulans is an excellent training immune inducer, and has good application prospect in the field of animal feed addition and disease immune prevention and control.

The application provides a method for preparing sialylated pullulan, which comprises the following steps: adding 1.0-30.0 g / L of sialic acid to a fermentation liquor of a strain of Aureobasidium pullulans EP1001 and / or a genetically engineered strain of Aureobasidium pullulans EP1001 after 24.0-60.0 hours of fermentation; catalyzing, by sialyltransferase, the transfer of sialic acid from an activated glycosyl donor to the end of a saccharide chain of a receptor pullulan to form a sialylated pullulan complex, i.e., sialylated pullulan; precipitating the supernatant of the centrifuged fermentation liquor by using an organic solvent; and finally obtaining the sialylated pullulan. The content of the pullulan in the sialylated pullulan is 55.05 g / L, the content of the sialylated pullulan in the sialylated pullulan is 9.58 g / L, the content of free sialic acid in the culture and / or the culture liquor is 0.42 g / L, and the sialic acid conversion rate is up to 95.8%.

The application discloses an MFS-1 transport protein gene and a method for improving the yield of pullulan, and belongs to the technical field of biology. The method comprises the following steps: obtaining the MFS-1 transport protein gene, wherein the nucleic acid sequence of the MFS-1 transport protein gene is shown as SEQ ID NO. 1, and the amino acid sequence is shown as SEQ ID NO. 2; transferring the MFS-1 transport protein gene into Aureobasidium pullulans to obtain an Aureobasidium pullulans pLH454-MFS-1 recombinant strain; and finally, fermenting the Aureobasidium pullulans pLH454-MFS-1 recombinant strain to obtain a fermentation liquor containing pullulan. The MFS-1 transport protein gene is obtained, the recombinant strain is constructed and then fermented, the fermentation time is shortened, the ability of the Aureobasidium pullulans to synthesize pullulan is improved, the pullulan is more efficiently, rapidly and intensively expressed and synthesized, the production cost of enterprises can be reduced, and therefore, the pullulan has a wider application space and prospect.

The invention relates to an aureobasidium pullulans engineering bacterium for producing high-molecular-weight pullulan and application of the aureobasidium pullulans engineering bacterium, and belongs to the technical field of biological engineering. According to the aureobasidium pullulans engineering bacterium disclosed by the invention, an alpha-amylase AMY gene, a glucamylase GLY gene and a pullulanase IPU gene are continuously knocked out, so that the molecular weight of pullulans produced by fermentation of aureobasidium pullulans is remarkably increased, and the problem that the molecular weight of pullulans produced by fermentation of aureobasidium pullulans in the prior art is uncontrollable is solved; and an excellent strain is provided for industrial production of high-molecular-weight pullulan polysaccharide. The molecular weight of pullulan produced by the aureobasidium pullulans engineering bacterium is greatly improved compared with that of a host strain and can be improved from 5.08 * 10 < 5 > Da to 5.1 * 10 < 6 > Da, the molecular weight can reach 1.31 * 10 < 7 > Da by optimizing fermentation conditions, and the pullulan can be widely applied to the fields of drug film-forming materials, food thickeners, stabilizers and the like.

This invention relates to a budding short-stem mold Cy28 and its applications, belonging to the field of microbial technology. This strain can reduce the Cd content in soil and produce more pullulan in Cd-containing environments. When applied to lettuce cultivation, it can significantly increase the dry weight of edible lettuce tissues and reduce the Cd content in edible lettuce tissues and roots, bringing the Cd content in edible lettuce tissues to edible standards, both in cadmium-free and cadmium-containing environments. Furthermore, it can increase the vitamin C, chlorophyll, soluble protein, and IAA content of edible lettuce tissues. Simultaneously, it can significantly increase beneficial microorganisms in the soil under cadmium conditions. Pseudarthrobacter The content of these elements helps to improve the soil.

Provided are an Aureobasidium pullulans CJFSKY2201 strain deposited under accession number KCCM13230P; a culture of the strain; use of the strain in producing oligosaccharides; a composition comprising the strain, a lysate thereof, a culture thereof, a concentrate thereof, or a dry product thereof; and a method of producing oligosaccharides, the method comprising the step of culturing the strain and the step of obtaining oligosaccharides from the culture.

The application belongs to the tobacco mildew prevention technical field, and particularly relates to application of essential oil slow-release beads in preparation of cigar leaf and cigarette mildew-proof products. The application provides application of essential oil slow-release beads in preparation of cigar leaf and cigarette mildew-proof products, wherein the essential oil slow-release beads comprise a bacteriostatic active substance and a slow-release carrier, the bacteriostatic component is continuously and slowly released through the coating effect of the slow-release carrier, thereby achieving a convenient mildew-proof method which does not need to be sprayed and can be directly placed with finished cigar cigarettes, the method does not affect the quality of cigars, can prolong the duration of the mildew-proof effect, and avoids the problem of short-term effect caused by one-time release; meanwhile, the mildew-proof slow-release preparation has a broad-spectrum bacteriostatic effect, can effectively inhibit the growth of various molds such as Aspergillus corntus, Aureobasidium pullulans and Scopulariopsis, and is suitable for long-term mildew-proof protection of the fermentation process of cigar leaves and the aging process of finished cigars.

The application relates to application of Aureobasidium pullulans Cy28 in relieving drought stress of plants and belongs to the technical field of microbial application; specifically, the application of Aureobasidium pullulans Cy28 in relieving drought stress of plants, wherein the strain preservation number of the Aureobasidium pullulans Cy28 is CCTCC NO: M 2025660; the inventor first finds that the Aureobasidium pullulans Cy28 has the effect of relieving drought stress of plants, can improve the drought resistance and quality of plants, and especially can not only improve the yield of wheat but also improve the protein content of wheat kernels and the amylose content of wheat kernels.

Provided are genetically modified strains of Aureobasidium pullulans that can produce a bioproduct which is heavy oil composed of polyol fatty acid esters (glycolipids, liamocin) with increased productivity and a method of producing a bioproduct using the same, wherein the genetically modified strain of Aureobasidium pullulans comprises an overexpression of a functional VHb protein and an overexpression of a functional PacC protein and is grown under conditions required to support the production of the bioproduct to collect the bioproduct in a high yield.

Provided are genetically modified strains of Aureobasidium pullulans that can produce a bioproduct with increased productivity and a method of producing a bioproduct using the same, wherein the genetically modified strain of Aureobasidium pullulans comprises an overexpression of a functional VHb protein and an overexpression of a functional PacC protein and is grown under conditions required to support the production of the bioproduct to collect the bioproduct in a high yield.

The invention belongs to the technical field of microbial fermentation, and particularly relates to aureobasidium pullulans NCPS-D9187 and application of the aureobasidium pullulans NCPS-D9187 in preparation of high-molecular-weight pullulan through fermentation. The aureobasidium pullulans NCPS-D9187 provided by the invention is preserved in the China Center for Type Culture Collection on November 17, 2025, and the preservation number is CCTCC NO: M 20252577. After a carbon source of a fermentation culture medium of the aureobasidium pullulans NCPS-D9187 is optimized, when the concentration of the carbon source is 250g / L, the molecular weight of pullulan produced by fermentation of the strain is up to 2.49 MDa, and the pullulan can be used for producing pullulan. Meanwhile, the yield of the pullulan is up to 189.67 g / L, and the molecular weight and the yield of the pullulan are synchronously increased.

A moisture-locking, film-forming, and multi-channel relieving and repairing compound, a preparation method therefor, and use thereof. The compound comprises: 0.1-15% of glycerin, 0.1-25% of a humectant, 0.01-0.5% of disodium ethylenediaminetetraacetic acid, 0.01-0.5% of polyquaternium, 0.01-0.5% of menthol, 0.01-2% of hydrogenated castor oil, 0.01-2% of a preservative, 0.01-0.1% of a European sour cherry flower extract, 0.01-0.1% of a bitter orange flower hydrolat, with the balance being deionized water; the humectant comprises 10-20 parts of sodium hyaluronate, 5-15 parts of aureobasidium pullulans polysaccharide, 5-10 parts of serine, and 1-5 parts of sodium alginate. The compound can be used for preparing skin care products such as sprays, creams, emulsions, and essences, and it features fast absorption and long-lasting moisturizing effects. At the same time, the product will not block the nozzle of a packaging container during long-term use.

The application discloses a bacillus velezensis for antagonizing chestnut blight fungus and an application thereof, the classification and naming of the bacillus velezensis is Bacillus velezensis G16, the preservation unit is China General Microbiological Culture Collection Center, the preservation number is CGMCC NO.33101, and the preservation time is December 31, 2024. Experimental results prove that the bacillus velezensis G16 has significant antagonistic activity on the chestnut blight fungus, the sterile fermentation supernatant of the bacillus velezensis G16 has certain control effect on chestnut branch infection of the chestnut blight fungus, and can obviously inhibit the expansion of a disease spot. In addition, the bacillus velezensis G16 provided by the application has good bacteriostatic activity on Aureobasidium pullulans, Colletotrichum gloeosporioides, Vitis phialangium, Lecanicillium laicium, Alternaria alternata and new shell crenospora, that is, the strain has the characteristics of inhibiting a broad spectrum, greatly increases the application range, and has a good development and application prospect.