57 results about "Aureobasidium pullulans" patented technology

The invention discloses aureobasidium pullulans, application thereof and a method for producing medium-molecular-weight pullulan, and belongs to the technical field of microorganisms. The aureobasidium pullulans disclosed by the invention is aureobasidium pullulans SYCY-018, the strain is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on December 26, 2022, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.40467. According to the aureobasidium pullulans SYCY-018, the aureobasidium pullulans SYCY-018 and the aureobasidium pullulans The aureobasidium pullulans SYCY-018 is inoculated into a fermentation medium for fermentation to produce the pullulan, the yield of the medium-molecular-weight pullulan reaches up to 150 g / L or above, meanwhile, good hereditary stability and high temperature resistance are achieved, the yield of the pullulan can still be higher than 130 g / L even at the high temperature of 40 DEG C, and the aureobasidium pullulans SYCY-018 is suitable for industrial production.

The present invention discloses an endophytic fungus Aureobasidium melanogenum producing melanin and its application. The strain of the present invention can effectively promote the growth of Arabidopsis thaliana and tomatoes. After co-culturing with this strain, the fresh weights of Arabidopsis thaliana and tomato seedlings are increased by 259.18% and 182.55% respectively compared with the control group; the leaf area of Arabidopsis thaliana seedlings is increased by 154.08% compared with the control group. After applying the filtrate of the fermentation broth of this strain, in the pot experiment for promoting growth, the fresh weight of the above-ground part of tomato seedlings is increased by 69.15% compared with the control group, the dry weight of the above-ground part is increased by 75.95%, the fresh weight of the underground part is increased by 80.00%, the dry weight of the underground part is increased by 120.01%, and the root length of tomato seedlings is increased by 66.98% compared with the control group. By applying the strain and the filtrate of its fermentation broth provided by the present invention, a significant growth-promoting effect can be exerted, and it has broad prospects for development and utilization in the field of agricultural microbial fertilizers and is worthy of vigorous promotion.

The invention provides aureobasidium pullulans F1 and application of the aureobasidium pullulans F1 in co-production of pullulan and beta-glucan, and belongs to the technical field of biological fermentation. The aureobasidium pullulans F1 is preserved in the China General Microbiological Culture Collection Center on March 17, 2025, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.41835; the invention relates to an application of aureobasidium pullulans F1 in co-production of pullulan and beta-glucan. The strain aureobasidium pullulans F1 provided by the invention can co-produce pullulan and beta-glucan, the yield of pullulan and beta-glucan is high, and the molecular weight of pullulan is low.

Disclosed in the present specification is a hypoallergenic composition for skin regeneration or skin soothing, containing, as an active ingredient, an Aureobasidium pullulans strain, a lysate thereof, a culture product thereof, or an extract of the strain, lysate or culture product. Unlike conventional functional ingredients, the composition is non-irritating to the skin, and thus is safe, while having a skin regeneration effect, and has a skin soothing effect by relieving irritation.

The invention relates to aureobasidium pullulans Cy28 and application thereof, and belongs to the technical field of microorganisms, the aureobasidium pullulans Cy28 can reduce the content of Cd in soil and can generate more pullulan in a Cd-containing environment; when the culture medium is applied to lettuce planting, the dry weight of edible tissues of the lettuce can be remarkably increased in a cadmium-free or cadmium-containing environment, and the content of Cd in the edible tissues and roots of the lettuce is reduced, so that the content of Cd in the edible tissues of the lettuce reaches the edible standard; in addition, the vitamin C content, the chlorophyll content, the soluble protein content and the IAA content of edible tissues of the lettuce can be improved; meanwhile, the content of beneficial microorganism Pseudarthrobacter in the soil can be remarkably increased in a cadmium environment, and the effect of improving the soil is achieved.

The present invention provides a budding Acidiflora pullulan F1 strain and an application thereof in the co-production of pullulan and β-glucan, belonging to the field of biofermentation technology; specifically, a budding Acidiflora pullulan F1 strain is provided, which was deposited in the General Microbiology Center of the China Culture Collection of Microorganisms on March 17, 2025, with the deposit address being No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number being CGMCC NO.41835; an application of budding Acidiflora pullulan F1 in the co-production of pullulan and β-glucan; the strain budding Acidiflora pullulan F1 provided by the present invention can co-produce pullulan and β-glucan, with high yields of both, and the pullulan molecular weight is low.

The present invention relates to the use of an isolated Aureobasidium pullulans yeast strain YBCA5 as a biological control agent. Processes and compositions for the biological control of phytopathogenic bacteria and fungi using YBCA5 are also provided.

The invention discloses a preparation method of a biodegradable film based on nanocellulose enhancement, and relates to the field of films. Hydroxyl groups on the surface of the nano cellulose fiber and Pullulan polysaccharide secreted by aureobasidium pullulans are self-assembled through hydrogen bonds to form a nano network structure; formaldehyde and hydrazide groups of hydrazide hyaluronic acid are subjected to a condensation reaction to generate acylhydrazone bonds, and a highly-crosslinked compact barrier layer is formed on the surface layer of the film; a polyphenol structure of tannic acid and a metal ion Fe < 3 + > fill gaps of nano cellulose fibers through coordination, so that the porosity is remarkably reduced, an oxygen diffusion path is prolonged, and the oxygen permeability is reduced; a hydrogen bond network is formed by the nano cellulose fibers and hydroxyl groups of the Pullulan polysaccharide through surface hydroxyl groups, meanwhile, terminal amino groups of the amino-terminated polycaprolactone and hydroxyl groups of the nano cellulose fibers generate ion-dipole interaction, and a three-dimensional interpenetrating structure is constructed; the tensile strength of the film can be effectively improved.

The present invention relates to the use of a cosmetic active ingredient comprising an extract of the biomass of at least one yeast of the species Aureobasidium pullulans, characterized in that the extract contains at least 25% by weight of sugars based on the dry matter of the extract, as a cosmetic active ingredient in a use or composition comprising the same.

Disclosed herein are methods of producing Massoia lactone from liamocins, or mixtures of liamocins and exophilins. Liamocins utilizable in the methodologies provided herein can be produced by microbes, such as Aureobasidium pullulans. The methodologies described herein utilize acid catalyzed hydrolysis / dehydration and carboxylic acids as a phase transfer agent in a dual-solvent system. This approach further allows the separation and collection of liamocin head groups.

The invention belongs to the technical field of gene engineering, and particularly relates to aureobasidium pullulans with high yield of pullulan and application of the aureobasidium pullulans. On the basis of aureobasidium pullulans M233-20, an isopullulanase gene A9187 gene in a genome of the aureobasidium pullulans M233-20 is knocked out based on a homologous recombination technology, and through polysaccharide fermentation test detection, an isopullulanase gene deletion mutant strain aureobasidium pullulans delta A9187-1 with the pullulan polysaccharide molecular weight improved and the yield not reduced is obtained. The weight-average molecular weight of the pullulan produced by the recombinant strain is 6.5 * 10 < 5 > Da, which is 2.6 times that of the original wild strain (2.46 * 10 < 5 >), and the yield of the pullulan is increased from 147.33 g / L to 156 g / L, which is increased by 5.88%.

The invention provides a compound preparation for inhibiting aspergillus flavus and application of the compound preparation in prevention and treatment of aspergillus flavus pollution, and the compound preparation comprises a compound microbial agent and a compound extract preparation, the compound microbial agent is prepared by mixing the following microbial agents in parts by weight: 5-10 parts of bacillus sorola, 5-10 parts of bacillus amyloliquefaciens and 3-8 parts of black yeast; the composite extract preparation is prepared by mixing a winged bean extract and a vanilla bean extract according to a weight ratio of (30-55): 1. The plant extract and the microbial agent are compounded to inhibit the aspergillus flavus, the antibacterial effect is good, and a new direction is provided for preventing and treating the aspergillus flavus of grain and oil crops and grain products.

The purpose of the present invention is to efficiently extract mycosporine-like amino acids (MAAs) from the cells of black yeast of the family Dothioraceae of the family Dothioraceae, and to obtain an MAAs extract at a high concentration. According to the present invention, provided is a method for extracting MAAs into water, said method comprising a culture step for culturing aureobasidium pullulans and an extraction step for extracting a MAAs fraction from cultured cells of the aureobasidium pullulans, and satisfying one or more of the following (a), (b), and (c): (a) in the extraction step, the MAAs fraction is extracted from the cultured cells of the aureobasidium pullulans in the extracted cells of the aureobasidium pullulans in the extracted cells of the aureobasidium pullulans in the extracted cells of the aureobasidium pullulans in the extracted cells of the aureobasidium pullulans in the extracted cells of the aureobasidium pullulans; the MAAs fraction is extracted in a polar solvent at a temperature above 40 DEG C. (b) Before the extraction step, a heating step is performed in which the thalli are collected from the culture solution after the culture step and the collected thalli are heated. (c) In the culturing step, culturing is performed in a culture medium that does not substantially contain ascorbic acid or a salt thereof and / or peptone.

The problem of the present invention is to provide: a method for stably producing β-1,3 / 1,6-glucan at a high yield from the Aureobasidium pullulans N-163 strain; and β-1,3 / 1,6-glucan produced by said method. This problem is solved by the discovery of the ability to obtain β-1,3 / 1,6-glucan stably at a high yield by using water that contains a large amount of silicon in the form of silicic acid in a step in which the Aureobasidium pullulans N-163 strain (accession number: NITE BP-03377) is cultured.　

The present disclosure provides an Aureobasidium pullulans CJFSKY2201 strain deposited under accession number KCCM13230P; a culture of the strain; use of the strain in producing oligosaccharides; a composition comprising the strain, a lysate thereof, a culture thereof, a concentrate thereof, or a dry product thereof; and a method of producing oligosaccharides, the method comprising the step of culturing the strain and the step of obtaining oligosaccharides from the culture.

The invention provides aureobasidium pullulans G1 and application of the aureobasidium pullulans G1 in co-production of pullulan and beta-glucan, and belongs to the technical field of biological fermentation. The aureobasidium pullulans G1 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 17, 2025, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.41837; the invention relates to an application of aureobasidium pullulans G1 in co-production of pullulan and beta-glucan. The strain aureobasidium pullulans G1 provided by the invention can co-produce pullulan and beta-glucan, and the yield of pullulan and beta-glucan is high.

The present application provides a strain of Aureobasidium pullulans E1 and its application in co-production of pullulan and beta-glucan, and belongs to the technical field of biological fermentation; specifically provides a strain of Aureobasidium pullulans E1, which is preserved in the China General Microbiological Culture Collection Center on March 17, 2025, the preservation address is No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.41836; the application of Aureobasidium pullulans E1 in co-production of pullulan and beta-glucan; the strain of Aureobasidium pullulans E1 provided by the present application can co-produce pullulan and beta-glucan, and the yield of both is high.

The invention provides a method for monitoring the synthesis efficiency of pullulan in a fermentation process of aureobasidium pullulans in real time based on a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) technology. A quantitative correlation model of gene expression abundance and polysaccharide yield is established by specifically detecting transcription dynamics of key genes (such as GluK-1, PGM-2, UGT-1 and AmAGS2-2) for synthesizing pullulan polysaccharide. Compared with chemical detection means such as high performance liquid chromatography (HPLC), the method has the advantages of high sensitivity, short time consumption and multi-target parallel analysis, and an innovative molecular tool is provided for intelligent monitoring and yield increase in the industrial fermentation process.

The present invention discloses Aureobasidium pullulans and its application and a method for producing medium-molecular-weight pullulan polysaccharide, belonging to the technical field of microorganisms. The Aureobasidium pullulans of the present invention is Aureobasidium pullulans ( Aureobasidium pullulans ) SYCY-018, which was deposited in the General Microbiological Center of the China Committee for Culture Collection of Microorganisms on December 26, 2022, with the deposit number of CGMCC NO. 40467. In the present invention, the Aureobasidium pullulans SYCY-018 is inoculated into a fermentation medium for fermentation to produce pullulan polysaccharide. The yield of medium-molecular-weight pullulan polysaccharide is as high as over 150 g / L, and it also has good genetic stability and high temperature resistance. Even at a high temperature of 40 °C, the yield of pullulan polysaccharide can still be higher than 130 g / L, which is suitable for industrial production.

The present invention relates to the use of a cosmetic active ingredient comprising at least one biomass extract of a yeast of the Aureobasidium pullulans species, characterized in that the extract comprises at least 25% sugar by weight of the dry matter of the extract, or the use of a composition comprising the extract as a cosmetic active ingredient.

The invention discloses a compound microbial agent rich in beta-glucan as well as a preparation method and application of the compound microbial agent, and belongs to the technical field of agricultural biology. According to the microbial agent, an aureobasidium pullulans-rhizobium pullulans-bacillus subtilis-bacillus thuringiensis quaternary synergistic system is constructed, specific auxiliary materials, carriers and a coating technology are supplemented, and under the drought stress condition, the microbial agent can remarkably promote the growth (plant height and biomass) of the pakchoi, so that the yield of the pakchoi is increased, and the yield of the pakchoi is increased. The yield of the pakchoi is increased, the quality is improved (the content of soluble sugar and protein is increased), the chlorophyll content and the photosynthetic efficiency are improved, the antioxidant enzyme activity is enhanced, cell membrane damage is reduced, the physical and chemical properties of soil are improved, and a new effective solution is provided for agricultural green drought resistance.

The application provides an Aureobasidium pullulans G1 and application thereof in co-production of pullulan and beta-glucan, and belongs to the technical field of biological fermentation; specifically provides an Aureobasidium pullulans G1, which is preserved in the China General Microbiological Culture Collection Center on March 17, 2025, and the address is No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.41837; application of the Aureobasidium pullulans G1 in co-production of pullulan and beta-glucan; the Aureobasidium pullulans G1 provided by the application can co-produce pullulan and beta-glucan, and the yield of the two is high.

The application discloses application of melanin-producing Aureobasidium pullulans CNBG-PGPF-1 in improving saline soil and / or plant salt tolerance and / or promoting plant growth and belongs to the technical field of microorganisms.The strain CNBG-PGPF-1 has a promoting effect on seed germination and seedling growth of Medicago polymorpha under the condition of saline soil.The strain CNBG-PGPF-1 also enhances the salt stress tolerance of Medicago polymorpha by reducing the content of sodium ions, increasing the content of potassium ions, reducing the accumulation of hydrogen peroxide and increasing the content of proline.The melanin-producing Aureobasidium pullulans and the microbial agent thereof have a good application prospect in the improvement of saline soil and the production of crops in saline soil, and provide resource and technical support for further development of microbial agents or microbial manure suitable for the improvement of saline soil and the agricultural production in saline soil.

The present application provides a mutant glucanase, which has a mutation at the amino acid position corresponding to position 121 of SEQ ID No. 2 compared to the parent glucanase. The enzyme activity of the mutant glucanase of the present application is increased by 68% compared to the wild-type enzyme, and has a wide application prospect in degrading materials containing glucan (for example, Aureobasidium pullulans residues produced in the production of pullulan).

The application discloses a method for regulating high-efficiency expression of heterologous genes of Aureobasidium melanogenum based on rDNA integration vectors and application, first constructs integration vectors pAMrDNA1, pAMrDNA2 and pAMrDNA3 targeting three different regions of rDNA, compares integration efficiency by taking gfp as a report gene, and selects the optimal site rDNA1.On this basis, endogenous pks genes of Aureobasidium melanogenum are first knocked out, then pks gene back complementation integration is mediated by the pAMrDNA1 vector, stable multi-copy expression is realized, and further 11.0 kb melanin biosynthesis gene clusters are integrated into a three-gene knockout chassis strain by the pAMrDNA1 vector in one step, the highest 15.0 copy integration is realized, and melanin yield is increased by about 12 times.The application realizes stable and high-efficiency expression of heterologous genes of Aureobasidium melanogenum, and especially provides an effective tool for one-step integration of large fragment gene clusters.

Disclosed herein are methods of producing massoia lactone from liamocins, or mixtures of liamocins and exophilins. Liamocins utilizable in the methodologies provided herein can be produced by microbes, such as Aureobasidium pullulans. The methodologies described herein utilize acid catalyzed hydrolysis / dehydration and carboxylic acids as a phase transfer agent in a dual-solvent system. This approach further allows the separation and collection of liamocin head groups.

The invention relates to an aureobasidium pullulans engineering bacterium for producing low-molecular-weight pullulan and application of the aureobasidium pullulans engineering bacterium, and belongs to the technical field of biological engineering. According to the aureobasidium pullulans engineering bacterium disclosed by the invention, the alpha-amylase AMY gene, the glucamylase GLY gene and the pullulanase IPU gene are continuously overexpressed, so that the molecular weight of pullulan produced by fermenting the aureobasidium pullulans is remarkably reduced, and the fermentation titer of the strain is improved; the problem that the molecular weight of pullulan produced through fermentation of aureobasidium pullulans in the prior art is uncontrollable is solved, an excellent strain is provided for preparing pullulan through a microbial fermentation method, the separation cost in the downstream purification process of pullulan is reduced, an excellent strain is provided for industrial production of low-molecular-weight pullulan, and the method is suitable for industrial production of pullulan. The molecular weight of pullulan produced after the strain is fermented for 3 days is 9.2 * 10 < 3 > Da and is greatly reduced compared with the molecular weight 5.08 * 10 < 5 > Da of pullulan produced by a host strain, the molecular weight can be reduced to 3.6 * 10 < 3 > Da by optimizing a fermentation culture medium and culture conditions, and the pullulan can be widely applied to the fields of food additives, preparation of cosmetic raw materials and the like.

The invention discloses an aureobasidium sp. Strain ZX033 for producing fumaric acid and gluconic acid and application of the aureobasidium sp. Strain ZX033. The preservation number of the aureobasidium sp. Strain ZX033 is CCTCC M 20252559. The invention also provides a separation and identification method of the strain. The ITS sequence and the LSU sequence of the strain are respectively shown as SEQ ID NO.1 and SEQ ID NO.2. Experiments prove that the strain can simultaneously produce fumaric acid and gluconic acid through fermentation. Furthermore, a glucose oxidase (GOX) gene of the ZX033 strain is successfully knocked out through a genetic engineering means, and an engineering strain ZX033-delta GOX is constructed. The fumaric acid production capacity of the engineering strain is remarkably improved, compared with an original strain, the yield in a specific culture medium can be improved by 28.7% or above, and the engineering strain has better industrial application potential. The invention provides a new strain resource and a fermentation process for efficiently producing fumaric acid and gluconic acid.

The invention belongs to the technical field of disease control, and relates to a compound microbial fermentation composition. The compound microbial fermentation composition disclosed by the invention is prepared from the following effective components in parts by weight: 50 to 130 parts of fermentation liquor of Chinese microbacterium, 60 to 110 parts of fermentation liquor of aureobasidium pullulans and 15 to 55 parts of plant exosome, wherein the fermentation liquor of the Chinese microbacterium is the fermentation liquor of the Chinese microbacterium SM8, and the preservation number of the Chinese microbacterium SM8 is GMCCNo.15757; the fermentation liquor of the aureobasidium pullulans is the fermentation liquor of the aureobasidium pullulans PA-2, and the preservation number of the aureobasidium pullulans PA-2 is CGMCC (China General Microbiological Culture Collection Center) No.8413; the plant exosome is a clematis exosome. The compound microbial fermentation composition disclosed by the invention can be used for preventing and treating root-knot nematodes.

The present application relates to the application of extracellular beta-glucan from Aureobasidium pullulans in preparing training immune inducer. By establishing mouse bone marrow derived macrophage (BMDMs) and zebrafish larvae training immune activation evaluation model, the immune activity of extracellular beta-glucan (eBG) from Aureobasidium pullulans is evaluated. It is found that compared with cell wall derived beta-glucan (cBG), the extracellular beta-glucan (eBG) from Aureobasidium pullulans has more significant training immune activation effect, which proves that the extracellular beta-glucan (eBG) from Aureobasidium pullulans is an excellent training immune inducer, and has good application prospect in the field of animal feed addition and disease immune prevention and control.