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181 results about "Aureobasidium pullulans" patented technology
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Aureobasidium pullulans is a ubiquitous black, yeast-like fungus that can be found in different environments (e.g. soil, water, air and limestone). It is well known as a naturally occurring epiphyte or endophyte of a wide range of plant species (e.g. apple, grape, cucumber, green beans, cabbage) without causing any symptoms of disease. A. pullulans has a high importance in biotechnology for the production of different enzymes, siderophores and pullulan. Furthermore, A. pullulans is used in biological control of plant diseases, especially storage diseases.
Choline-utilizing microbial strains for biologically controlling fusarium head blight
InactiveUS7601346B1Effective suppressionEffective controlBiocideBacteriaTriticeaeFusarium ear blight
Three choline utilizing strains of microorganisms isolated from the anthers of wheat, Aureobasidium pullulans strain AS 55.2, Arthrobacter species strain OH 221.3, and Pseudomonas species strain AS 64.4, are superior antagonists of F. graminearum. These microorganisms are effective for suppression and control of FHB in cereals, particularly in wheat and barley.
Owner:AGRI THE UNITED STATES OF AMERICAS AS REPRESENTED BY THE SEC OF +1
Mutant aureobasidium pullulans TKPM00006 for massively producing beta-poly(malic acid) and culture method thereof
ActiveCN101979499AHigh yieldSimplify the screening processFungiMutant preparationPullulanAureobasidium
The invention provides mutant aureobasidium pullulans TKPM00006 for massively producing beta-poly(malic acid) and a method for producing poly(malic acid) by using the mutant through fermentation. The strain is the mutant for producing beta-poly(malic acid) with high yield, which is cultured by multiple groups of composite mutagenesis technology based on beta-poly(malic acid) producing TKPM10017 screened in the soil of Wang Taibao orchard, Yinchuan, Ningxia Hui Autonomous Region, China. Under the optimization condition, the yield of beta-poly(malic acid) reaches 18.4+ / -1.3g / l.
Owner:天津慧智百川生物工程有限公司
Composition containing beta-glucan and constipation-relieving drug, immunopotentiatior, and skin moistening agent using the composition
InactiveUS20050272694A1Organic active ingredientsCosmetic preparationsAureobasidiumBULK ACTIVE INGREDIENT
The present invention provides a composition containing β-glucan in which the physiologically active effects of β-1,3-1,6-glucan contained in a cultured composition of a bacterium belonging to the genus Aureobasidium sp. are further enhanced, and a constipation-relieving drug, preparation an immunopotentiator, and a skin moistening agent using the composition. A composition containing β-glucan, which contains a cultured composition containing β-1,3-1,6-glucan obtained by culturing a bacterium belonging to the genus Aureobasidium sp. and lactic acid bacterium cells, is obtained. This composition containing β-glucan is employed as an active ingredient of a constipation-relieving drug, an immunopotentiator, or a skin moistening agent. Aureobasidium pullulans M-1 (FERM BP-08615) is preferable as the bacterium belonging to the genus Aureobasidium sp. Moreover, Enterococcus faecalis is preferable as the lactic acid bacterium. It is still preferable that the lactic acid bacterium has been killed by heating. Furthermore, the content of the cultured composition in solid matters preferably ranges from 1 to 80% by mass in terms of β-1,3-1,6-glucan, while the content of the lactic acid bacterium cells preferably ranges from 4 to 95% by mass.
Owner:AUREO CO LTD
Fire-retardant polyethylene cable material with mould-proof function and preparation method thereof
InactiveCN101570610AGood anti-mold functionImprove performancePlastic/resin/waxes insulatorsInsulated cablesLinear low-density polyethylenePullulan
Owner:SHANGHAI KAIBO SPECIAL CABLE FACTORY
Prussian blue producing strain and prussian blue producing process with the strain
InactiveCN1609188ALight colorImprove polysaccharide yieldFungiFermentationPullulanWater soluble polysaccharides
The present invention relates to utilizing saccharide metabolism to produce extracellular water soluble polysaccharide and belongs to the field of bioengineering technology. The present invention provides strain, named Aureobasidium pullulans G-58 and with the preservation number of CGMCC No. 1234. The pullulan producing process with the strain includes fermentation culture, fermented liquid post-treatment, heat treatment, decolorizing, precipitation, desalting, drying and other steps. The pullulan product has light color, high polysaccharide content, excellent viscoelasticity, excellent emulsifying performance and plasticity, and no toxicity and harm, and may be used widely in food, medicine, cosmetics, etc.
Owner:JIANGNAN UNIV
Bacterial strain for producing PMLA [Poly (Beta-L-malic acid)] and method for producing PMLA by fermentation of bacterial strain
InactiveCN102220248AEfficient fermentationThe post-processing process is simpleFungiMicroorganism based processesPullulanMicrobiology
The invention discloses a bacterial strain for producing PMLA [Poly (Beta-L-malic acid)], which is obtained by screening the surface of a plant leaf and can not produce melanin. The classification name is Aureobasidium pullulans, the Latin literature name is Aureobasisdium pullulans ZD-3D, and the preservation number of the bacterial strain is CGMCC No.4605. The method for producing PMLA by the bacterial strain is simple to implement and is beneficial to the separation and purification of PMLA, and the yield can be up to 50-60g / L.
![Bacterial strain for producing PMLA [Poly (Beta-L-malic acid)] and method for producing PMLA by fermentation of bacterial strain](https://images-eureka.patsnap.com/patent_img/bd7d7ade-f1e4-41b3-8906-fdfc7168172e/110520142403.PNG "Bacterial strain for producing PMLA [Poly (Beta-L-malic acid)] and method for producing PMLA by fermentation of bacterial strain")
Owner:SHANDONG JINCHENG BIO PHARMA CO LTD
Comprehensive application method of salicornia bigelovii
The invention relates to a comprehensive application of salicornia bigelovii. The salicornia bigelovii comprises fresh vegetables and tender stems of plants; active substances are extracted after the salicornia bigelovii is pulped or dried and crushed; the active substances comprise components such as polysaccharide, ferulic acid, general flavone and the like; the residues of salicornia bigelovii after the active substances are extracted are inoculated with food-security microorganism aureobasidium pullulans for fermentation so as to produce fermented products rich in single-cell protein (SCP) and viscous pullulan which has been allowed by the FDA (food and drug administration) in Japan to be used as a food additive, the pullulan produced by self fermentation is utilized as a binding agent, infrared and microwave technologies are adopted to dewater, dry and form fermentation mud, and the fermented products are prepared into instant vegetable paper with rich nutrition, so that the variety of leisure foods is richened and the high-additional-value comprehensive utilization system of the salicornia bigelovii is constructed.
Owner:YANCHENG INST OF TECH
Aureobasidium pullulans and application thereof
InactiveCN102994395AAchieve synthesisMeeting the demand for high molecular weight pullulanFungiMicroorganism based processesBiotechnologyPullulan
The invention discloses Aureobasidium pullulans and application thereof. The Aureobasidium pullulans strain SZU1001 is preserved in China center for type culture collection (CCTCC), and the preservation number is CCTCC M 2012259. A method for producing pullulan by the Aureobasidium pullulans strain includes the steps of by using the Aureobasidium pullulans strain as an original strain, performing fermentation cultivation for at least 48 hours after slope seed activation and seed culture, and extracting with an organic solvent to obtain the pullulan. The method is characterized in that the fermentation medium per liter contains glucose 40-60g, yeast powder 0.8-3.5g, ammonium sulfate 0.2-1.0g, dipotassium phosphate 1.0-3.0g, magnesium sulfate 0.1-0.3g, and sodium chloride 0-1.0g, and the pH value is 6.5-6.8. According to the invention, the aureobasidium pullulans mutant strain which can synthesize glutathione in cells can be obtained, so that the synthesis of pullulan with high molecular weight and no pigment production is realized, thereby meeting the requirement of application fields, such as medicine and environment, on the pullulan.
Owner:SUZHOU UNIV
Method for producing poly(malic acid) and pullulan together by using Aureobasidium pullulans
ActiveCN102492740AIncrease profitSave energyMicroorganism based processesFermentationSucrosePullulan
The invention discloses a method for producing poly(malic acid) and pullulan together by using Aureobasidium pullulans. The invention relates to a method for producing poly(malic acid) and pullulan by using Aureobasidium pullulans, belonging to the technical field of the bio-fermentation engineering. The method comprises the following steps: reducing the temperature of the culture medium of a fermentation tank to 28 DEG C, inoculating a strain in the fermentation tank according to the inoculation quantity which is 3%, stirring under the conditions that the rotational speed is 200rpm and the ventilation rate is 0.5VVM, controlling the temperature to 28-31 DEG C to begin fermentation, performing the fed-batch of sucrose after 24 hours to control the content of sucrose in the fermentation liquor to 35g / L, controlling the temperature to about 25 DEG C after 72 hours, continuously performing the fed-batch of sucrose to control the content of sucrose in the fermentation liquor to 15 g / L, fermenting for 136 hours to stop adding sucrose, continuously fermenting for 8 hours when the content of sucrose in the fermentation liquor is reduced to 0, controlling the pH value to 5-7.2 in the fermentation process, and fermenting for 166 hours. By adopting the method disclosed by the invention, the raw material utilization rate can be increased, energy can be saved and the cost can be reduced.
Owner:天津北洋百川生物技术有限公司
Method for increasing pulullan yield
InactiveCN103695500ALow costImprove conversion rateMicroorganism based processesFermentationBiotechnologyPullulan
The invention discloses a method for increasing pulullan yield by sub-sectional adding of growth factors in a fermentation process, and belongs to the technical field of biological fermentation engineering. The method comprises the following steps: producing pulullan from aureobasidium pullulans CGMCC NO.7055 in a fermentation manner; adding 0.003-0.005% of vitamin B1 when bacteria growth is at the beginning of the logarithmic phase; and adding 0.002-0.004% of uridine monophosphate when the bacteria growth is at the early stage of a stable stage, so as to induce and facilitate accumulation of secondary metabolite, namely the pulullan. The growth factors are specifically added according to the characteristics of sugar production in different growth periods and fermentation processes, so that the method is convenient and fast to operate and has an obvious effect; the fermentation period is greatly shortened, the transformation rate of a substrate is increased, and the cost of the pulullan is reduced.
Owner:天津北洋百川生物技术有限公司
Method for improving poly-malic acid yield
ActiveCN103789363AIncrease productionRealize the flowMicroorganism based processesFermentationPullulanCarbon metabolism
The invention discloses a method for improving the poly-malic acid yield in aureobasidium pullulans through surface active agent tween or span. The method particularly comprises the steps that after the aureobasidium pullulans is activated, the aureobasidium pullulans is fermented under the condition containing 0.1 g / L to 0.5 g / L tween or span. The method is simple, the tween or the span can be directly added into a fermentation medium, the carbon metabolism flow direction of synthesis of the poly-malic acid and Pulullan can be adjusted, the poly-malic acid yield can be improved to a large extent, and the method has the advantages of being low in fermentation cost, high in yield, strong in technical economical efficiency, capable of being applied to poly-malic acid industrial production and the like.
Owner:SOUTHWEST UNIV
Method for biological catalysis of unsymmetrical reduction carbon based compound in water/ion liquid diphasic system
InactiveCN101319236AEasy to separateEasy to recycleMicroorganism based processesFermentationHydroxybutyric acidBiocompatibility Testing
The invention discloses a method for asymmetrically reducing a carbonyl compound by biocatalysis in a water / ion liquid biphasic system, which belongs to the biochemical engineering technical field. The method takes a selective strain producing carbonyl reductase as a starting strain, and prochiral ketone as a substrate to perform reducing preparation of corresponding chiral alcohol in the water / ion liquid biphasic system; the method comprises the following steps that: Aureobasidium pullulans CGMCC No.1244 is used to catalyze 4-chloro-acetoacetic acid ethyl ester to perform the asymmetrical reduction preparation of (S)-4-chloro-3-hydroxybutyric acid ethyl ester, and Saccharoinyces uvarum ATCC 26602 is used to catalyze 4,4,4-trifluoroacetoacetate to perform the asymmetrical reduction preparation of (R)-4, 4, 4-trilfluoro-3-hydroxybutyrate. The method shows the advantages of no toxicity, no smell, difficult volatilization, good biocompatibility, no environmental pollution, simple product separation, easy recovery, repeated use and so on, of ion liquid. At the same time, the method improves the transformation rate, the concentration and an enantiomeric excess value of a reaction product, and quickens the process of the reaction.
Owner:JIANGNAN UNIV
Salicornia paper type vegetable and preparation method
InactiveCN103584005AImprove food safetyFull of nutritionFood ingredient as thickening agentFood thermal treatmentNutrients proteinPullulan
The invention discloses salicornia vegetable paper and a production method of the salicornia vegetable paper. The method is characterized in that aureobasidium pullulans fermented salicornia is utilized to produce the salicornia vegetable paper. The method comprises the following basic processes: selecting fresh salicornia; cleaning; retaining color; pulping; homogenizing; seasoning and sterilizing; inoculating aureobasidium pullulans to ferment; paying on a plate to mold; baking; removing a film; segmenting; and packing. The preparation method of the vegetable paper has the advantages that an outsourcing thickening agent is not needed, and polysaccharide produced by aureobasidium pullulans fermentation is used as an endogenous thickening agent, thus the content of nutrient protein of the vegetable paper is increased, the preparation cost is decreased, and the safety in taking is improved; and the vegetable paper remains the taste of fresh salicornia, and is convenient to take, the problem that fresh salicornia is difficult to store is solved, the variety of snack foods is enriched, and the added value of the use of salicornia is also increased.
Owner:射阳县射阳港渔工贸开发有限公司
Liquid plastic mulch and preparation method thereof
ActiveCN104403135AEasy to operateGood effectClimate change adaptationMicroorganism based processesBacillus licheniformisPullulan
The invention discloses a liquid plastic film, and belongs to the technical field of agriculture. The liquid plastic film consists of the following components in percentage by weight: 1%-10% of pullulan polysaccharide, 1%-10% of polyglutamic acid and the balance of water, wherein the pullulan polysaccharide is prepared by fermenting aureobasidium pullulans of which the preservation No. is CGMCC No.7055, and the polyglutamic acid is prepared by fermenting bacillus licheniformis. The invention further discloses a preparation method and a use method of the liquid plastic film. The liquid plastic film is biodegradable and does not pollute environment, and after the plastic film is sprayed onto the surface of soil, the temperature of the soil can be raised, the evaporation of water is restrained, water content of the soil is increased, the salt content of the soil is reduced, and the soil can also be improved, so that the physicochemical property of the soil is better, and the growth of plants is promoted; besides, the liquid plastic film has the advantages of simple preparation method, low cost, good convenience in use, saving of labors and material, low cost and easiness in obtaining of raw materials, simple production equipment and technology, zero pollution in a production process, and the like.
Owner:天津北洋百川生物技术有限公司
Production method of pullulan without pigment
The invention relates to a fermentation production method of pullulan without pigment. Aureobasidium pullulans, which are used as the starting strain, are induced by microwaves to obtain the mutant strain with high-yield pullulan and little pigment secretion. The fermentation production method of the pullulan comprises the following steps: (1) preparing seeds and fermentation medium; (2) inoculating the activated strain to the seed culture medium according to the inoculum size of 2-10%, and culturing at 28+ / -1 DEG C for 24-36 hours, wherein the concentration of seeds is 0.8-2.5*108 / mL; (3) inoculating the seed liquid to the fermentation medium according to the inoculum size of 2-10%, pressurizing to 0.01-0.05 Mpa in a tank at 28+ / -1 DEG C, adjusting the stirring rate and ventilation amount at different fermentation stages and fermenting for 60-72 hours; and (4) after-treatment of fermentation liquid: using decoloration of the fermentation liquid, plate-and-frame filter, ceramic membrane ultrafiltration ultrafiltering for the sugar solution and the other methods to carrying out ultrafiltration and concentratuion, and performing spray drying to obtain the pullulan product.
Owner:INST OF BIOPHARM OF SHANDONG PROVINCE
Collagen skin firming lifting plumping glowing cream
InactiveCN107233282ALighten the dullnessEasy to synthesizeCosmetic preparationsToilet preparationsPullulanHydrolysis
The invention discloses a collagen skin firming lifting plumping glowing cream which comprises red ginseng snail secreta filter liquor, soluble collagen, hydrolysis collagen, atelocollagen, sodium hyaluronate, caesalpinia spinosa fruit extracts / Kappaphycus alvarezii extracts, nicotinamide, mica, hydrogenated phosphatidylcholine, lysoph-osphatidylcholine / sclerotium gum / xanthan gum / aureobasidium pullulans polysaccharide, mentha piperita extracts, hydroxyethyl acrylate / acryloyl dimethyl sodium taurocholate copolymers. Periocular skins are firmed, lifted and glowed from outside to inside by the aid of the cream. Ray refraction modes are adjusted by the aid of the mica, so that wrinkles are flatted, skins are brightened, melanin can be restrained by the nicotinamide, black eyes are faded, 'external regulation and internal nourishing' are achieved, the periocular skins are rapidly and effectively brightened and rapidly firmed and lifted, the hydrogenated phosphatidylcholine serves as emulsifying agents and thickening agents, mild and non-irritating effect is achieved, the mentha piperita extracts bring cool and refreshing skin feeling, fatigue is relieved, generation of fat granules is prevented, and the healthy state of the skins is kept.
Owner:NOX BELLCOW COSMETICS CO LTD
Strain producing pullulan, application thereof and pullulan production method
InactiveCN105695347AQuality improvementIncrease productionFungiMicroorganism based processesBiotechnologyMicroorganism
The invention relates to the field of microorganisms and discloses aureobasidium pullulans.The preservation number of the aureobasidium pullulans is CGMCC No.11937.The aureobasidium pullulans with the preservation number being CGMCC No.11937 uses wild aureobasidium pullulans with the preservation number being CGMCC No.3.4580 as an original strain, chemical induced mutation is performed, the aureobasidium pullulans is obtained through screening, the capacity that the aureobasidium pullulans ferments to generate pullulans is remarkably improved, compared with the original strain, the yield of the pullulans is increased by 80%, and the glucose conversion rate is increased by 68%.The color of the finished pullulans becomes lighter obviously.Thus, the aureobasidium pullulans with the preservation number being CGMCC No.11937 can be widely applied to pullulans fermentation.
Owner:MEIHUA BIOTECH LANGFANG CO LTD
Collagen nano instant mask taking aureobasidium pullulans polysaccharide as framework and preparation method
ActiveCN109674685AHigh porosityLarge specific surface areaCosmetic preparationsToilet preparationsPullulanMass ratio
The invention discloses a collagen nano instant mask taking aureobasidium pullulans polysaccharide as a framework and a preparation method. The mask comprises a non-woven fabric base material layer and a nano-fiber layer, wherein the nano-fiber layer is formed on the non-woven fabric base material layer by adopting an electrostatic spinning method; the nano-fiber layer is prepared from the following components in parts by weight: 10 to 60 parts of the aureobasidium pullulans polysaccharide, 5 to 40 parts of hydrolyzed collagen with relatively low molecular weight and 0.1 to 5 parts of a surface active substance; the average molecular weight of the aureobasidium pullulans polysaccharide is 100000 to 300000; the average molecular weight of the hydrolyzed collagen with relatively low molecular weight is 1000 to 3000; the mass ratio of the aureobasidium pullulans polysaccharide to the hydrolyzed collagen with relatively low molecular weight ranges from (1 to 5) to (6 to 1).
Owner:NOX BELLCOW COSMETICS CO LTD
Cosmetic additive with anti-pollution effect and cosmetic thereof
The invention discloses a cosmetic additive with an anti-pollution effect and a cosmetic thereof, and aims to provide the cosmetic additive capable of effectively reducing adhesion to pollutants to the skin, protect skin cells, resisting external pollutants and having the effects of inflammation resisting, repairing and relieving. The cosmetic additive comprises, by weight, 3-12 parts of braseniaschreberi leaf extract, 1-10 parts of quercus robur root extract, 1-7 parts of moringa pterygosperma seed extract, 2-8 parts of salicornia herbacea extract, 1-6 parts of aureobasidium pullulans fermentation product and 2-7 parts of ginkgo biloba extract. The cosmetic additive can be used in cosmetics to enhance the skin barrier and achieve excellent isolation and defense functions on the skin andhas a good application prospect. The cosmetic belongs to the technical field of cosmetics.
Owner:GUANGDONG BAWEI BIOLOGICAL TECH CO LTD
Skin-care composition and preparation method thereof
InactiveCN107496231AGum supplementFree radical scavengingCosmetic preparationsToilet preparationsPullulanBetaine
The invention discloses a skin-care composition. The skin-care composition comprises a solvent, substrate solution, a thickening agent, an emulsifying agent, a pH modifier, a preservative and the following conditioning components: glycine betaine, mycose, aureobasidium pullulans polysaccharide, ROSA MULTIFLORA fruit extractive, ROAS RUGOSA oil, SCLEROTIUM ROLFSSII glue, sodium hyaluronate and hydrolyzed collagen. The skin-care composition can form a layer of moisturizing anti-cracking protection barrier with good weather resistance, and effectively remove free radical on the surface and surface layer of skin, and has the effects of oxidation resistance, skin whitening and aging resistance; the skin-care composition can effectively supplement skin collagen, and give skin elasticity and gloss; the skin-care composition cooperatively preserves moisture, keeps skin white and resists skin aging. A preparation method of the skin-care composition is beneficial to the obtaining of the skin-care composition uniform in preparation and capable of being properly absorbed by skin.
Owner:广州市馨雨化妆品有限公司
Nitrogen, phosphorus and potassium microbial coated fertilizer and preparation method thereof
InactiveCN106187503AIncrease profitAvoid cakingSuperphosphatesAlkali orthophosphate fertiliserPullulanMicrobial agent
The invention discloses a nitrogen, phosphorus and potassium microbial coated fertilizer and a preparation method thereof. The nitrogen, phosphorus and potassium microbial coated fertilizer comprises a pill core, a middle layer and a mycoderm layer from inside to outside in sequence; the pill core comprises the following raw materials: urea, monopotassium phosphate, diammonium phosphate, calcium superphosphate, potassium sulphate, plant ash and glutinous rice glue; the middle layer comprises the following raw materials: furfural residues, vinasse, sorghum residues, edible fungus culture medium wastes, aureobasidium pullulans, EM (effective microorganism) bacterial liquid and clay; the mycoderm layer comprises the following raw materials: peat soil, a microbial agent, Carbomer, 10 wt% sodium hydroxide aqueous solution and water. The nitrogen, phosphorus and potassium microbial coated fertilizer disclosed by the invention has a viable count of more than 0.062 billion CFU / g, an organic content of more than 60.5 wt%, a pH value of 7.1-7.2 and an N, P and K content ratio of (2-3.5):(1.5-2.1):1; the reasonable collocation is performed according to nitrogen, phosphorus, potassium and other demands of crops, so that the release period of fertilizer nutrients can be prolonged; the microbial agent improves the soil texture and the tillage performance and inhibits the emergence of weeds and pests. The preparation method of the fertilizer has the advantages that the raw materials are easy to obtain, the operation is simple, the biological survival rate is high, the activity is good, and the use effect is outstanding.
Owner:SHANDONG ZHIFENG NONGHUA GRP
Aureobasidium pullulans for highly producing pigment-free Pulullan and method for culturing and preparing Pulullan
The invention discloses an aureobasidium pullulans for highly producing pigment-free Pulullan, which is collected in China Center For Type Culture Collection (CCTCC) with a collection number of CCTCC M2013611. The invention further discloses a mutation screening method and a culture method of aureobasidium pullulans for highly producing pigment-free Pulullan, and a method for producing Pulullan through fermentation of the aureobasidium pullulans for highly producing pigment-free Pulullan. A culture obtained by fermentation cultivation of an aureobasidium pullulans strain GM-1 cannot show a dark green color or deep green color due to accumulation of melanin substance, but form a milk white or faint yellow composition. Compared with a conventional Pullulan producing strain, the aureobasidium pullulans strain GM-1 has the advantages that the Pulullan is colorless and is higher in yield, the purposes of increasing the raw material utilization rate and reducing the production cost are reached, and the demand of application fields such as foods, medicines, and environment on the pigment-free Pulullan is met.
Owner:INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
Method for producing novel natural melanin
ActiveCN102517335AIncrease productionIncrease profitMicroorganism based processesFermentationPullulanMelanin
The invention discloses a method for producing novel natural melanin. The method is characterized by comprising the following steps of: activating aureobasidium pullulans for 4 to 8 hours, performing shaking culture for 12 to 36 hours to obtain seed liquid, inoculating the seed liquid in a volume of which is 1 to 10 percent of that of a fermentation medium to the fermentation medium, and performing liquid state fermentation; centrifuging the fermentation solution obtained by fermentation for 5 minutes under the condition of 5,000r / min, performing acidolysis on the supernate by using hydrochloric acid, and then performing precipitation by using methanol to obtain a crude melanin product; dissolving the crude product to obtain a black solution by using an alkali solution; and performing precipitation on the obtained black solution by using hydrochloric acid, and thus obtaining the melanin. Compared with other fermentation medium, the method using the fermentation medium has the advantages that: the yield of the melanin is improved from 2.47g / L to 10.74g / L, the fermentation period is shortened from 7 days to 3 days, the culture substrate is fully utilized, the utilization rate of raw materials is improved, and the production cost is reduced.
Owner:天津北洋百川生物技术有限公司
Aureobasidium pullulans and applications thereof
ActiveCN107058123AGrowth inhibitionPromote growthFungiMicroorganism based processesBiotechnologyPullulan
The invention provides aureobasidium pullulans and applications thereof. The collection number of the strain is CGMCC No.12249, and marine yeast aureobasidium pullulans HJM01 is applied to agricultural production as a microbial fertilizer. The screened marine yeast aureobasidium pullulans and active substances in fermented liquor of the aureobasidium pullulans serve as the microbial fertilizer, the fertilizer can effectively inhibit and prevent the occurrence of diseases of botrytis cinerea on vegetables of tomatoes and the like after being applied to soil, the strain can decompose insoluble phosphate to produce soluble phosphate after producing acid in the soil, and phytase produced from the strain can decompose phytic acid in the soil to produce soluble phosphate, so that the soil fertility can be increased and the growth of plants can be promoted.
Owner:OCEAN UNIV OF CHINA
Aureobasidium pullulans and method for fermenting to produce pulullan thereof
ActiveCN107699500AIncrease productionReduce manufacturing costFungiMicroorganism based processesBiotechnologySnow mold
The invention belongs to the technical field of microbes and in particular relates to an aureobasidium pullulans stain NCPS 2016-M capable of producing pulullan at high yield. The aureobasidium pullulans stain NCPS 2016-M capable of producing pulullan at high yield provided by the invention is characterized in that the strain is collected in China Center for Type Culture Collection Center on September 14, 2017, and has a collection number of CCTCC M 2017498. The aureobasidium pullulans stain disclosed by the invention has the beneficial effects that the amount of the pulullan produced by performing shaking flask fermentation on the aureobasidium pullulans stain provided by the invention reaches 60g / L, and the ability of producing the pullulans is 1.5-1.7 times that of a wild aureobasidiumpullulans stain. The mutant strain does not produce any melanin, and the fermentation time is shortened by six hours compared with that of an original strain. The technical problem that wide application of the pullulans in the domestic food industry, particularly in an agricultural product preservation aspect, is greatly limited because the pullulans production level is low and the product qualityis low is solved.
Owner:INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
Aureobasidium pullulans, culturing medium and method for producing b-glucan, a culture of aureobasidium pullulans and a composition comprising the same
ActiveUS20180187226A1High yieldWithout melaninFungiMicroorganism based processesPullulanStaphylococcus cohnii
The present application provides a culture medium for producing β-glucan. The culture medium comprises: a carbon source, lecithin and an ascorbic acid. The present application further provides a method for producing β-glucan, the method comprising culturing Aureobasidium pullulans in the culture medium for fermentation. The Aureobasidium pullulans is advantageous in producing no melanin; as a result, the yield of β-glucan can be increased and the waste can be reduced with the improved culture medium composition and culturing method.
Owner:FOOD IND RES & DEV INST
Aureobasidium pullulans strain and application thereof
ActiveCN109136109AEfficient fermentationThe post-processing process is simpleFungiMicroorganism based processesMicroorganismPullulan
The invention relates to an Aureobasidium pullulans strain and an application thereof, and belongs to the technical fields of fermentation engineering and biology. The preservation name of the Aureobasidium pullulans strain is Aureobasidium pullulans ZK-502, the strain is preserved in CGMCC (China General Microbiological Culture Collection Center) on August 20, 2018 at No.3 of No.1 West Beichen Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 16326. The Aureobasidium pullulans strain can efficiently produce liamocin by fermentation and produces no melanin, fermentation broth is light pink to faint yellow, and follow-up treatment process can be simplified. The method is simple and feasible, raw materials are simple in composition, yield of liamocin can reach 8-10 g / L, and commercial and large-scale production of liamocin is expected to be realized; the obtained liamocin product is dissoluble in water and has wide application prospect in medicine, cosmetics andfood.
Owner:优鲜工坊(浙江)食品有限公司
Microbial preparation for treating tobacco stems and preparation method thereof
ActiveCN103451106AIncrease the amount of aromaIncrease pleasureFungiTobacco treatmentPullulanIrritation
The invention discloses a microbial preparation for tobaccos and a preparation method and application thereof, belonging to the technical field of tobacco additives. According to the preparation method, the corresponding microbial preparation is obtained through carrying out liquid fermentation culture on producing strains, wherein the producing strains are aureobasidium pullulans OF-01 strains (CGMCC (China General Microbiological Culture Collection Center) No.7609). The microbial preparation disclosed by the invention has the advantages that the characters, such as aroma amount, pleasantness and the like, of cut stems can be improved obviously, offensive odors, irritation and the like are reduced, the concentration and momentum of smoke are reduced, and the aftertaste becomes comfortable, so that the blended amount of the cut stems in cigarettes is further increased, and the aims of reducing harm, lowering tar content and reducing the cigarette production cost are achieved.
Owner:HONGYUN HONGHE TOBACCO (GRP) CO LTD
Method for increasing yield of pullulan
InactiveCN105624233AIncrease productionIncrease production capacityFermentationBiotechnologyPullulan
The invention discloses a method for increasing the yield of pullulan. The method comprises the following steps: after activating aureobasidium pullulans, inoculating a seed medium; after OD600 is greater than 0.3, inoculating seeds into a fermentation medium; monitoring OD600 of a fermentation liquid in real time; when OD600 is greater than 0.2 and less than 0.3, adding a compounding agent I which accounts for 0.02-0.03% of the volume of the fermentation liquid, so as to greatly increase the bacterium growth velocity and remarkably shorten the logarithmic growth phase; when OD600 is greater than or equal to 0.6, adding a compounding agent II which accounts for 0.03-0.05% of the volume of the fermentation liquid. The substrate conversion speed can be accelerated, the polysaccharide synthesis performance can be improved, and the yield can be greatly increased.
Owner:天津北洋百川生物技术有限公司
Method for increasing pulullan output through fed-batch fermentation
InactiveCN104911231AHigh substrate conversion rateAids in synthesisMicroorganism based processesFermentationPullulanAnimal science
The invention relates to a method for increasing pulullan output through fed-batch fermentation, which comprises the following steps: culturing seeds, performing fermentation culture and separating pulullan; continuously feeding a fed-batch solution after fermenting for 24-28 hours, controlling the residual sugar of a broth to maintain at 6-12g / L, supplementing 0.5-2g / L of amino acid solution for once by every two hours, wherein the total fermentation period is 65-67 hours. Aiming at defects of long period and low substrate conversion rate during a process of fermentation of aureobasidium pullulans with a preservation number of No. 7055 to produce pulullan, a proper amount of amino acid is continuously fed during the fermentation process for synthesis of pullulan, substrate conversion rate is increased, operation is convenient, effect is obvious, fermentation period is greatly shortened, and cost of pullulan can be reduced.
Owner:天津北洋百川生物技术有限公司
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