200 results about "Aureobasidium" patented technology

The invention relates to a method used microorganism enzyme resolution DL-pantoic acid lactone to produce D-pantoic acid. It uses the D-pantoic acid lactone hydrolase strain of the fusarium, gibberella, aspergillus, penicillium, rhizopus, gliocladium, aureobasidium to ferment and culture, uses wet thallus as coarse enzyme, DL-pantoic acid lactone as substrate to produce D-pantoic acid. L- pantoic acid lactone can be reclaimed. The DL-pantoic acid lactone gained by racemization reaction can newly be used to do resolution.

The invention provides mutant aureobasidium pullulans TKPM00006 for massively producing beta-poly(malic acid) and a method for producing poly(malic acid) by using the mutant through fermentation. The strain is the mutant for producing beta-poly(malic acid) with high yield, which is cultured by multiple groups of composite mutagenesis technology based on beta-poly(malic acid) producing TKPM10017 screened in the soil of Wang Taibao orchard, Yinchuan, Ningxia Hui Autonomous Region, China. Under the optimization condition, the yield of beta-poly(malic acid) reaches 18.4+/-1.3g/l.

The present invention relates to utilizing saccharide metabolism to produce extracellular water soluble polysaccharide and belongs to the field of bioengineering technology. The present invention provides strain, named Aureobasidium pullulans G-58 and with the preservation number of CGMCC No. 1234. The pullulan producing process with the strain includes fermentation culture, fermented liquid post-treatment, heat treatment, decolorizing, precipitation, desalting, drying and other steps. The pullulan product has light color, high polysaccharide content, excellent viscoelasticity, excellent emulsifying performance and plasticity, and no toxicity and harm, and may be used widely in food, medicine, cosmetics, etc.

The invention discloses a bacterial strain for producing PMLA [Poly (Beta-L-malic acid)], which is obtained by screening the surface of a plant leaf and can not produce melanin. The classification name is Aureobasidium pullulans, the Latin literature name is Aureobasisdium pullulans ZD-3D, and the preservation number of the bacterial strain is CGMCC No.4605. The method for producing PMLA by the bacterial strain is simple to implement and is beneficial to the separation and purification of PMLA, and the yield can be up to 50-60g/L.

The invention relates to a comprehensive application of salicornia bigelovii. The salicornia bigelovii comprises fresh vegetables and tender stems of plants; active substances are extracted after the salicornia bigelovii is pulped or dried and crushed; the active substances comprise components such as polysaccharide, ferulic acid, general flavone and the like; the residues of salicornia bigelovii after the active substances are extracted are inoculated with food-security microorganism aureobasidium pullulans for fermentation so as to produce fermented products rich in single-cell protein (SCP) and viscous pullulan which has been allowed by the FDA (food and drug administration) in Japan to be used as a food additive, the pullulan produced by self fermentation is utilized as a binding agent, infrared and microwave technologies are adopted to dewater, dry and form fermentation mud, and the fermented products are prepared into instant vegetable paper with rich nutrition, so that the variety of leisure foods is richened and the high-additional-value comprehensive utilization system of the salicornia bigelovii is constructed.

The invention discloses an aureobasidium pullulan for producing pullulan, and belongs to the field of microorganisms. The preservation number of the aureobasidium pullulan BCSWGHPL101 (Aureobsidium pullulans) is CGMCCNO.7055; the fermentation medium adopted by the aureobasidium pullulan for producing the pullulan comprises peptone of 3g/L, cane sugar of 100g/L, K2HPO4.7H2O of 3.0g/L, MgSO4.7H2O of 0.4g/L, NaCl of 2.5g/L and FeSO of 40.01g/L, and the pH is adjusted to 7.0. Compared with an ordinary pullulan producing strain, the mutant strain BCSWGHPL101 is higher in pullulan production capacity, and as a result, the yield of the pullulan is effectively increased, and the production cost is lowered.

The invention discloses a method for producing poly(malic acid) and pullulan together by using Aureobasidium pullulans. The invention relates to a method for producing poly(malic acid) and pullulan by using Aureobasidium pullulans, belonging to the technical field of the bio-fermentation engineering. The method comprises the following steps: reducing the temperature of the culture medium of a fermentation tank to 28 DEG C, inoculating a strain in the fermentation tank according to the inoculation quantity which is 3%, stirring under the conditions that the rotational speed is 200rpm and the ventilation rate is 0.5VVM, controlling the temperature to 28-31 DEG C to begin fermentation, performing the fed-batch of sucrose after 24 hours to control the content of sucrose in the fermentation liquor to 35g/L, controlling the temperature to about 25 DEG C after 72 hours, continuously performing the fed-batch of sucrose to control the content of sucrose in the fermentation liquor to 15 g/L, fermenting for 136 hours to stop adding sucrose, continuously fermenting for 8 hours when the content of sucrose in the fermentation liquor is reduced to 0, controlling the pH value to 5-7.2 in the fermentation process, and fermenting for 166 hours. By adopting the method disclosed by the invention, the raw material utilization rate can be increased, energy can be saved and the cost can be reduced.

The invention discloses a method for increasing pulullan yield by sub-sectional adding of growth factors in a fermentation process, and belongs to the technical field of biological fermentation engineering. The method comprises the following steps: producing pulullan from aureobasidium pullulans CGMCC NO.7055 in a fermentation manner; adding 0.003-0.005% of vitamin B1 when bacteria growth is at the beginning of the logarithmic phase; and adding 0.002-0.004% of uridine monophosphate when the bacteria growth is at the early stage of a stable stage, so as to induce and facilitate accumulation of secondary metabolite, namely the pulullan. The growth factors are specifically added according to the characteristics of sugar production in different growth periods and fermentation processes, so that the method is convenient and fast to operate and has an obvious effect; the fermentation period is greatly shortened, the transformation rate of a substrate is increased, and the cost of the pulullan is reduced.

The invention discloses aureobasidium pullulans dicarboxylic acid transport protein and a recombinant vector and application thereof. Amino acid sequence of the aureobasidium pullulans dicarboxylic acid transport protein is shown as SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9 or SEQ ID NO.10 while nucleotide sequence of the same is shown as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4 and SEQ ID NO.5. Overexpression of dicarboxylic acid transport protein in aureobasidium pullulans can increase yield of polymalic acid, and no free malic acid is detected in fermentation liquor, so that dicarboxylic acid transport protein gene is related to synthetic transport of the polymalic acid, and a foundation is laid for increasing yield of the polymalic acid.

The invention relates to a fermentation production method of pullulan without pigment. Aureobasidium pullulans, which are used as the starting strain, are induced by microwaves to obtain the mutant strain with high-yield pullulan and little pigment secretion. The fermentation production method of the pullulan comprises the following steps: (1) preparing seeds and fermentation medium; (2) inoculating the activated strain to the seed culture medium according to the inoculum size of 2-10%, and culturing at 28+/-1 DEG C for 24-36 hours, wherein the concentration of seeds is 0.8-2.5*108/mL; (3) inoculating the seed liquid to the fermentation medium according to the inoculum size of 2-10%, pressurizing to 0.01-0.05 Mpa in a tank at 28+/-1 DEG C, adjusting the stirring rate and ventilation amount at different fermentation stages and fermenting for 60-72 hours; and (4) after-treatment of fermentation liquid: using decoloration of the fermentation liquid, plate-and-frame filter, ceramic membrane ultrafiltration ultrafiltering for the sugar solution and the other methods to carrying out ultrafiltration and concentratuion, and performing spray drying to obtain the pullulan product.

The invention discloses an aureobasidium pullulans genome scale metabolism network model and application thereof. Construction of the aureobasidium pullulans genome scale metabolism network model comprises four steps of building of a network database, refining and calibrating of the database, building of a mathematical model and validation and analysis of the model. According to the constructed aureobasidium pullulans genome scale metabolism network model, a gene target for potentially improving polymalic acid can be predicted, a guiding platform is provided for research and comprehensive analysis of aureobasidium pullulans, and the aureobasidium pullulans genome scale metabolism network model has great significance on transformation of the aureobasidium pullulans to obtain high-yield metabolic products of the polymalic acid and pullulan.

The invention provides a complex preservative and application thereof in fruit and vegetable preservation. The preservative is prepared by the following steps: enabling a pururan solution to react with a gamma-polyglutamic acid solution, thereby preparing cross-linked polymer powder; dissolving the cross-linked polymer powder in distilled water, thereby obtaining a cross-linked polymer solution; and enabling the cross-linked polymer solution to react with a copper salt aqueous solution, thereby obtaining the product. The pururan is prepared by fermenting and extracting strong bud aureobasidium, and the gamma-polyglutamic acid is prepared by fermenting and extracting bacillus licheniformis. The complex preservative prepared by the invention has excellent film-forming property and moisture retention property and is extremely small in use amount, is capable of obviously improving the preservation effect of fruits and vegetables, is convenient to use and easy to operate, can be widely applied to perishable fruits and vegetables such as mangos, strawberries, young garlic shoot and chives stem and is suitable for fruit and vegetable preservation in fruit and vegetable producing areas, fruit and vegetable markets, factories/enterprises and families.

The invention discloses a medium capable of improving a polymalic acid yield. The medium contains 1 to 20g/L of citric acid or citrate. The medium can provide external power donors to aureobasidium pullulans in fermentation, and can improve a cellular ATP supply level thereby improving a polymalic acid yield maximally by 57.1%. The medium can be used for producing polymalic acid by aureobasidium pullulan fermentation and has good economic benefits.

The invention relates to the field of microorganisms and discloses aureobasidium pullulans.The preservation number of the aureobasidium pullulans is CGMCC No.11937.The aureobasidium pullulans with the preservation number being CGMCC No.11937 uses wild aureobasidium pullulans with the preservation number being CGMCC No.3.4580 as an original strain, chemical induced mutation is performed, the aureobasidium pullulans is obtained through screening, the capacity that the aureobasidium pullulans ferments to generate pullulans is remarkably improved, compared with the original strain, the yield of the pullulans is increased by 80%, and the glucose conversion rate is increased by 68%.The color of the finished pullulans becomes lighter obviously.Thus, the aureobasidium pullulans with the preservation number being CGMCC No.11937 can be widely applied to pullulans fermentation.

The invention discloses a collagen nano instant mask taking aureobasidium pullulans polysaccharide as a framework and a preparation method. The mask comprises a non-woven fabric base material layer and a nano-fiber layer, wherein the nano-fiber layer is formed on the non-woven fabric base material layer by adopting an electrostatic spinning method; the nano-fiber layer is prepared from the following components in parts by weight: 10 to 60 parts of the aureobasidium pullulans polysaccharide, 5 to 40 parts of hydrolyzed collagen with relatively low molecular weight and 0.1 to 5 parts of a surface active substance; the average molecular weight of the aureobasidium pullulans polysaccharide is 100000 to 300000; the average molecular weight of the hydrolyzed collagen with relatively low molecular weight is 1000 to 3000; the mass ratio of the aureobasidium pullulans polysaccharide to the hydrolyzed collagen with relatively low molecular weight ranges from (1 to 5) to (6 to 1).

The invention discloses a skin-care composition. The skin-care composition comprises a solvent, substrate solution, a thickening agent, an emulsifying agent, a pH modifier, a preservative and the following conditioning components: glycine betaine, mycose, aureobasidium pullulans polysaccharide, ROSA MULTIFLORA fruit extractive, ROAS RUGOSA oil, SCLEROTIUM ROLFSSII glue, sodium hyaluronate and hydrolyzed collagen. The skin-care composition can form a layer of moisturizing anti-cracking protection barrier with good weather resistance, and effectively remove free radical on the surface and surface layer of skin, and has the effects of oxidation resistance, skin whitening and aging resistance; the skin-care composition can effectively supplement skin collagen, and give skin elasticity and gloss; the skin-care composition cooperatively preserves moisture, keeps skin white and resists skin aging. A preparation method of the skin-care composition is beneficial to the obtaining of the skin-care composition uniform in preparation and capable of being properly absorbed by skin.

The invention discloses a nitrogen, phosphorus and potassium microbial coated fertilizer and a preparation method thereof. The nitrogen, phosphorus and potassium microbial coated fertilizer comprises a pill core, a middle layer and a mycoderm layer from inside to outside in sequence; the pill core comprises the following raw materials: urea, monopotassium phosphate, diammonium phosphate, calcium superphosphate, potassium sulphate, plant ash and glutinous rice glue; the middle layer comprises the following raw materials: furfural residues, vinasse, sorghum residues, edible fungus culture medium wastes, aureobasidium pullulans, EM (effective microorganism) bacterial liquid and clay; the mycoderm layer comprises the following raw materials: peat soil, a microbial agent, Carbomer, 10 wt% sodium hydroxide aqueous solution and water. The nitrogen, phosphorus and potassium microbial coated fertilizer disclosed by the invention has a viable count of more than 0.062 billion CFU/g, an organic content of more than 60.5 wt%, a pH value of 7.1-7.2 and an N, P and K content ratio of (2-3.5):(1.5-2.1):1; the reasonable collocation is performed according to nitrogen, phosphorus, potassium and other demands of crops, so that the release period of fertilizer nutrients can be prolonged; the microbial agent improves the soil texture and the tillage performance and inhibits the emergence of weeds and pests. The preparation method of the fertilizer has the advantages that the raw materials are easy to obtain, the operation is simple, the biological survival rate is high, the activity is good, and the use effect is outstanding.

The invention belongs to the technical field of microbes and in particular relates to an aureobasidium pullulans stain NCPS 2016-M capable of producing pulullan at high yield. The aureobasidium pullulans stain NCPS 2016-M capable of producing pulullan at high yield provided by the invention is characterized in that the strain is collected in China Center for Type Culture Collection Center on September 14, 2017, and has a collection number of CCTCC M 2017498. The aureobasidium pullulans stain disclosed by the invention has the beneficial effects that the amount of the pulullan produced by performing shaking flask fermentation on the aureobasidium pullulans stain provided by the invention reaches 60g/L, and the ability of producing the pullulans is 1.5-1.7 times that of a wild aureobasidiumpullulans stain. The mutant strain does not produce any melanin, and the fermentation time is shortened by six hours compared with that of an original strain. The technical problem that wide application of the pullulans in the domestic food industry, particularly in an agricultural product preservation aspect, is greatly limited because the pullulans production level is low and the product qualityis low is solved.

It is intended to utilize β-glucan produced by a bacterium belonging to Aureobasidium sp. From a bacterium belonging to Aureobasidium sp., a mutant with little pigment accumulation is constructed by a mutagenesis means of, for example, irradiating with ultraviolet light or treating with a mutagen. A culture obtained by culturing this mutant in a liquid culture medium is usable as a composition with a large β-glucan content without showing any intense dark green color caused by the accumulation of melanin-like pigments. This composition may be taken as such as a functional food having the physiologically active functions of the β-glucan-containing composition. Alternatively, it may be added to foods, drinks. food additives, cosmetics and so on.

The invention relates to an Aureobasidium pullulans strain and an application thereof, and belongs to the technical fields of fermentation engineering and biology. The preservation name of the Aureobasidium pullulans strain is Aureobasidium pullulans ZK-502, the strain is preserved in CGMCC (China General Microbiological Culture Collection Center) on August 20, 2018 at No.3 of No.1 West Beichen Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 16326. The Aureobasidium pullulans strain can efficiently produce liamocin by fermentation and produces no melanin, fermentation broth is light pink to faint yellow, and follow-up treatment process can be simplified. The method is simple and feasible, raw materials are simple in composition, yield of liamocin can reach 8-10 g/L, and commercial and large-scale production of liamocin is expected to be realized; the obtained liamocin product is dissoluble in water and has wide application prospect in medicine, cosmetics andfood.

The invention discloses a microbial preparation for tobaccos and a preparation method and application thereof, belonging to the technical field of tobacco additives. According to the preparation method, the corresponding microbial preparation is obtained through carrying out liquid fermentation culture on producing strains, wherein the producing strains are aureobasidium pullulans OF-01 strains (CGMCC (China General Microbiological Culture Collection Center) No.7609). The microbial preparation disclosed by the invention has the advantages that the characters, such as aroma amount, pleasantness and the like, of cut stems can be improved obviously, offensive odors, irritation and the like are reduced, the concentration and momentum of smoke are reduced, and the aftertaste becomes comfortable, so that the blended amount of the cut stems in cigarettes is further increased, and the aims of reducing harm, lowering tar content and reducing the cigarette production cost are achieved.