49 results about "Aureobasidium" patented technology

The invention discloses a method for gene modification of aureobasidium pullulans, which belongs to the technical field of gene engineering, and comprises the following steps: determining a malic acid synthase gene, obtaining a malic acid synthase gene segment, searching a gene sequence of the malic acid synthase in the disclosed aureobasidium pullulans as a template on NCBI (National Center of Biotechnology Information), and carrying out gene modification on the malic acid synthase gene segment to obtain a malic acid synthase gene segment; the method comprises the following steps: respectively designing an upstream primer and a downstream primer, taking extracted genome DNA of aureobasidium pullulans M1 as a template, respectively carrying out PCR amplification and recombinant plasmid pUC57-PMA-ms construction and linearization treatment on the upstream primer and the downstream primer, and introducing the plasmid into the aureobasidium pullulans M1 to obtain an aureobasidium pullulans recombinant strain; according to the method, the polymalic acid synthetase gene (PMA-ms) on an aureobasidium pullulans genome is knocked out from aureobasidium pullulans, the yield of pullulan in the obtained recombinant plasmid M1-2 is about 39.11 g / L, and compared with an original strain M1, the yield of M1-2 is increased by 9.79 g / L, and the practical application prospect of the method is improved.

The invention discloses a melanin-producing aureobasidium for co-producing pullulan and converting American ginseng and application, and relates to the technical field of bioengineering fermentation. The melanin producing aureobasidium is aureobasidium melanogenum F8449, and the preservation number of the aureobasidium melanogenum F8449 is CGMCC (China General Microbiological Culture Collection Center) No. 23228. The strain can be used for simultaneously producing pullulan and biologically converting American ginseng extract in liquid, so that efficient utilization of fermentation substances is realized, the fermentation cost is reduced, and the fermentation income is increased.

The invention provides aureobasidium pullulans F1 and application of the aureobasidium pullulans F1 in co-production of pullulan and beta-glucan, and belongs to the technical field of biological fermentation. The aureobasidium pullulans F1 is preserved in the China General Microbiological Culture Collection Center on March 17, 2025, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.41835; the invention relates to an application of aureobasidium pullulans F1 in co-production of pullulan and beta-glucan. The strain aureobasidium pullulans F1 provided by the invention can co-produce pullulan and beta-glucan, the yield of pullulan and beta-glucan is high, and the molecular weight of pullulan is low.

The invention discloses a modified aureobasidium pullulans strain based on reduction of malic acid pathway consumption and application of the modified aureobasidium pullulans strain. Gene for coding NADPH dependent malic enzyme and phosphoenolpyruvate carboxykinase in an aureobasidium pullulans genome is directionally knocked out. The transformation blocks a key consumption branch for synthesis and accumulation of the cytoplasmic malic acid, and effectively guides a central carbon metabolic flow to be more efficiently guided to a target product. Experimental results show that the polymalic acid yield and the saccharic acid conversion rate of the double-knockout engineering strain D3N5-delta3617 / deltapepck in fermentation are remarkably improved compared with those of an original strain, and the biomass is also increased. The invention provides a high-performance strain with important application value for industrial efficient fermentation production of polymalic acid.

The application discloses a malt PYF prediction method based on metagenome detection and belongs to the field of malt quality detection. Candida The relative abundance of Phytobacter The relative abundance of Curtobacterium The relative abundance of Kosakonia The relative abundance of Enterobacter The relative abundance of Fusarium The relative abundance of Aureobasidium The relative abundance of Acinetobacter The relative abundance of Klebsiella The relative abundance of The PYF potential is calculated, and the smaller the PYF potential is, the lower the degree of yeast pre-flocculation caused by the malt is. The application is applied to the yeast pre-flocculation degree prediction aspect, solves the problem that the existing malt PYF detection method cannot accurately reflect the yeast pre-flocculation condition in actual application, has the characteristics that the detection result is more accurate and can truly reflect the degree of yeast pre-flocculation caused by the malt.

This invention belongs to the field of resource utilization technology, specifically relating to a low-temperature composting self-heating start-up compound microbial agent, its preparation method, and its application. The low-temperature composting self-heating start-up compound microbial agent is composed of *Broodia buddingis* (…). Aureobasidium sp.X4-8) and Bacillus licheniformis ( Bacillus licheniformis The compound microbial agent (H-29) was prepared by combining two bacterial groups. The two bacterial groups exhibited dominance and functional complementarity at different temperature stages. When added at -30℃, the psychrophilic fungi initially "ignited" the heat, and as the compost temperature exceeded 40℃, the heat-resistant bacteria rapidly took over and released lignin peroxidase and manganese peroxidase, achieving continuous regulation of the composting system from initial heating and high-temperature maintenance to deep decomposition under low-temperature conditions. Aerobic composting of agricultural waste using the compound microbial agent resulted in a seed germination index (GI) ≥ 70% for the decomposed product, with all indicators meeting the NY / T 525-2021 standard.

The invention discloses a haematococcus culture dissolved oxygen coupling regulation and control method for a corrosion-resistant photoreactor, relates to the field of bioengineering, and builds a photoreactor made of a corrosion-resistant material and a matched regulation and control system; preparing a special improved BG-11 culture medium for haematococcus and injecting the culture medium into the photoreactor; oxygen transfer coefficients under different ventilation rates are calibrated through a dynamic method, a ternary equation of light intensity, cell density and oxygen production rate is fitted by combining photosynthetic characteristics of haematococcus, and a basic model for dynamic prediction of dissolved oxygen is established. According to the method, the photoreactor made of a corrosion-resistant material and a matched regulation and control system are built, so that the interference of material dissolution on the culture medium is effectively avoided, and the stability of a haematococcus culture environment is guaranteed. Based on a ternary fitting equation of an oxygen transfer coefficient calibrated by a dynamic method, light intensity, cell density and oxygen production rate, a basic model for dynamic prediction of dissolved oxygen is constructed, and accurate coupling regulation and control of dissolved oxygen are realized by combining model prediction control and reinforcement learning agents.

The invention relates to aureobasidium pullulans Cy28 and application thereof, and belongs to the technical field of microorganisms, the aureobasidium pullulans Cy28 can reduce the content of Cd in soil and can generate more pullulan in a Cd-containing environment; when the culture medium is applied to lettuce planting, the dry weight of edible tissues of the lettuce can be remarkably increased in a cadmium-free or cadmium-containing environment, and the content of Cd in the edible tissues and roots of the lettuce is reduced, so that the content of Cd in the edible tissues of the lettuce reaches the edible standard; in addition, the vitamin C content, the chlorophyll content, the soluble protein content and the IAA content of edible tissues of the lettuce can be improved; meanwhile, the content of beneficial microorganism Pseudarthrobacter in the soil can be remarkably increased in a cadmium environment, and the effect of improving the soil is achieved.

The present invention provides a budding Acidiflora pullulan F1 strain and an application thereof in the co-production of pullulan and β-glucan, belonging to the field of biofermentation technology; specifically, a budding Acidiflora pullulan F1 strain is provided, which was deposited in the General Microbiology Center of the China Culture Collection of Microorganisms on March 17, 2025, with the deposit address being No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number being CGMCC NO.41835; an application of budding Acidiflora pullulan F1 in the co-production of pullulan and β-glucan; the strain budding Acidiflora pullulan F1 provided by the present invention can co-produce pullulan and β-glucan, with high yields of both, and the pullulan molecular weight is low.

The present invention relates to the use of a cosmetic active ingredient comprising an extract of the biomass of at least one yeast of the species Aureobasidium pullulans, characterized in that the extract contains at least 25% by weight of sugars based on the dry matter of the extract, as a cosmetic active ingredient in a use or composition comprising the same.

The invention belongs to the technical field of gene engineering, and particularly relates to aureobasidium pullulans with high yield of pullulan and application of the aureobasidium pullulans. On the basis of aureobasidium pullulans M233-20, an isopullulanase gene A9187 gene in a genome of the aureobasidium pullulans M233-20 is knocked out based on a homologous recombination technology, and through polysaccharide fermentation test detection, an isopullulanase gene deletion mutant strain aureobasidium pullulans delta A9187-1 with the pullulan polysaccharide molecular weight improved and the yield not reduced is obtained. The weight-average molecular weight of the pullulan produced by the recombinant strain is 6.5 * 10 < 5 > Da, which is 2.6 times that of the original wild strain (2.46 * 10 < 5 >), and the yield of the pullulan is increased from 147.33 g / L to 156 g / L, which is increased by 5.88%.

The purpose of the present invention is to efficiently extract mycosporine-like amino acids (MAAs) from the cells of black yeast of the family Dothioraceae of the family Dothioraceae, and to obtain an MAAs extract at a high concentration. According to the present invention, provided is a method for extracting MAAs into water, said method comprising a culture step for culturing aureobasidium pullulans and an extraction step for extracting a MAAs fraction from cultured cells of the aureobasidium pullulans, and satisfying one or more of the following (a), (b), and (c): (a) in the extraction step, the MAAs fraction is extracted from the cultured cells of the aureobasidium pullulans in the extracted cells of the aureobasidium pullulans in the extracted cells of the aureobasidium pullulans in the extracted cells of the aureobasidium pullulans in the extracted cells of the aureobasidium pullulans in the extracted cells of the aureobasidium pullulans; the MAAs fraction is extracted in a polar solvent at a temperature above 40 DEG C. (b) Before the extraction step, a heating step is performed in which the thalli are collected from the culture solution after the culture step and the collected thalli are heated. (c) In the culturing step, culturing is performed in a culture medium that does not substantially contain ascorbic acid or a salt thereof and / or peptone.

The present disclosure provides an Aureobasidium pullulans CJFSKY2201 strain deposited under accession number KCCM13230P; a culture of the strain; use of the strain in producing oligosaccharides; a composition comprising the strain, a lysate thereof, a culture thereof, a concentrate thereof, or a dry product thereof; and a method of producing oligosaccharides, the method comprising the step of culturing the strain and the step of obtaining oligosaccharides from the culture.

The invention provides aureobasidium pullulans G1 and application of the aureobasidium pullulans G1 in co-production of pullulan and beta-glucan, and belongs to the technical field of biological fermentation. The aureobasidium pullulans G1 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 17, 2025, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.41837; the invention relates to an application of aureobasidium pullulans G1 in co-production of pullulan and beta-glucan. The strain aureobasidium pullulans G1 provided by the invention can co-produce pullulan and beta-glucan, and the yield of pullulan and beta-glucan is high.

The present application provides a strain of Aureobasidium pullulans E1 and its application in co-production of pullulan and beta-glucan, and belongs to the technical field of biological fermentation; specifically provides a strain of Aureobasidium pullulans E1, which is preserved in the China General Microbiological Culture Collection Center on March 17, 2025, the preservation address is No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.41836; the application of Aureobasidium pullulans E1 in co-production of pullulan and beta-glucan; the strain of Aureobasidium pullulans E1 provided by the present application can co-produce pullulan and beta-glucan, and the yield of both is high.

The invention provides a method for monitoring the synthesis efficiency of pullulan in a fermentation process of aureobasidium pullulans in real time based on a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) technology. A quantitative correlation model of gene expression abundance and polysaccharide yield is established by specifically detecting transcription dynamics of key genes (such as GluK-1, PGM-2, UGT-1 and AmAGS2-2) for synthesizing pullulan polysaccharide. Compared with chemical detection means such as high performance liquid chromatography (HPLC), the method has the advantages of high sensitivity, short time consumption and multi-target parallel analysis, and an innovative molecular tool is provided for intelligent monitoring and yield increase in the industrial fermentation process.

The invention discloses a compound microbial agent rich in beta-glucan as well as a preparation method and application of the compound microbial agent, and belongs to the technical field of agricultural biology. According to the microbial agent, an aureobasidium pullulans-rhizobium pullulans-bacillus subtilis-bacillus thuringiensis quaternary synergistic system is constructed, specific auxiliary materials, carriers and a coating technology are supplemented, and under the drought stress condition, the microbial agent can remarkably promote the growth (plant height and biomass) of the pakchoi, so that the yield of the pakchoi is increased, and the yield of the pakchoi is increased. The yield of the pakchoi is increased, the quality is improved (the content of soluble sugar and protein is increased), the chlorophyll content and the photosynthetic efficiency are improved, the antioxidant enzyme activity is enhanced, cell membrane damage is reduced, the physical and chemical properties of soil are improved, and a new effective solution is provided for agricultural green drought resistance.

The application provides an Aureobasidium pullulans G1 and application thereof in co-production of pullulan and beta-glucan, and belongs to the technical field of biological fermentation; specifically provides an Aureobasidium pullulans G1, which is preserved in the China General Microbiological Culture Collection Center on March 17, 2025, and the address is No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.41837; application of the Aureobasidium pullulans G1 in co-production of pullulan and beta-glucan; the Aureobasidium pullulans G1 provided by the application can co-produce pullulan and beta-glucan, and the yield of the two is high.

The application discloses application of melanin-producing Aureobasidium pullulans CNBG-PGPF-1 in improving saline soil and / or plant salt tolerance and / or promoting plant growth and belongs to the technical field of microorganisms.The strain CNBG-PGPF-1 has a promoting effect on seed germination and seedling growth of Medicago polymorpha under the condition of saline soil.The strain CNBG-PGPF-1 also enhances the salt stress tolerance of Medicago polymorpha by reducing the content of sodium ions, increasing the content of potassium ions, reducing the accumulation of hydrogen peroxide and increasing the content of proline.The melanin-producing Aureobasidium pullulans and the microbial agent thereof have a good application prospect in the improvement of saline soil and the production of crops in saline soil, and provide resource and technical support for further development of microbial agents or microbial manure suitable for the improvement of saline soil and the agricultural production in saline soil.

The present application provides a mutant glucanase, which has a mutation at the amino acid position corresponding to position 121 of SEQ ID No. 2 compared to the parent glucanase. The enzyme activity of the mutant glucanase of the present application is increased by 68% compared to the wild-type enzyme, and has a wide application prospect in degrading materials containing glucan (for example, Aureobasidium pullulans residues produced in the production of pullulan).

The application discloses a method for regulating high-efficiency expression of heterologous genes of Aureobasidium melanogenum based on rDNA integration vectors and application, first constructs integration vectors pAMrDNA1, pAMrDNA2 and pAMrDNA3 targeting three different regions of rDNA, compares integration efficiency by taking gfp as a report gene, and selects the optimal site rDNA1.On this basis, endogenous pks genes of Aureobasidium melanogenum are first knocked out, then pks gene back complementation integration is mediated by the pAMrDNA1 vector, stable multi-copy expression is realized, and further 11.0 kb melanin biosynthesis gene clusters are integrated into a three-gene knockout chassis strain by the pAMrDNA1 vector in one step, the highest 15.0 copy integration is realized, and melanin yield is increased by about 12 times.The application realizes stable and high-efficiency expression of heterologous genes of Aureobasidium melanogenum, and especially provides an effective tool for one-step integration of large fragment gene clusters.

Disclosed herein are methods of producing massoia lactone from liamocins, or mixtures of liamocins and exophilins. Liamocins utilizable in the methodologies provided herein can be produced by microbes, such as Aureobasidium pullulans. The methodologies described herein utilize acid catalyzed hydrolysis / dehydration and carboxylic acids as a phase transfer agent in a dual-solvent system. This approach further allows the separation and collection of liamocin head groups.

The invention relates to an aureobasidium pullulans engineering bacterium for producing low-molecular-weight pullulan and application of the aureobasidium pullulans engineering bacterium, and belongs to the technical field of biological engineering. According to the aureobasidium pullulans engineering bacterium disclosed by the invention, the alpha-amylase AMY gene, the glucamylase GLY gene and the pullulanase IPU gene are continuously overexpressed, so that the molecular weight of pullulan produced by fermenting the aureobasidium pullulans is remarkably reduced, and the fermentation titer of the strain is improved; the problem that the molecular weight of pullulan produced through fermentation of aureobasidium pullulans in the prior art is uncontrollable is solved, an excellent strain is provided for preparing pullulan through a microbial fermentation method, the separation cost in the downstream purification process of pullulan is reduced, an excellent strain is provided for industrial production of low-molecular-weight pullulan, and the method is suitable for industrial production of pullulan. The molecular weight of pullulan produced after the strain is fermented for 3 days is 9.2 * 10 < 3 > Da and is greatly reduced compared with the molecular weight 5.08 * 10 < 5 > Da of pullulan produced by a host strain, the molecular weight can be reduced to 3.6 * 10 < 3 > Da by optimizing a fermentation culture medium and culture conditions, and the pullulan can be widely applied to the fields of food additives, preparation of cosmetic raw materials and the like.

The invention discloses an aureobasidium sp. Strain ZX033 for producing fumaric acid and gluconic acid and application of the aureobasidium sp. Strain ZX033. The preservation number of the aureobasidium sp. Strain ZX033 is CCTCC M 20252559. The invention also provides a separation and identification method of the strain. The ITS sequence and the LSU sequence of the strain are respectively shown as SEQ ID NO.1 and SEQ ID NO.2. Experiments prove that the strain can simultaneously produce fumaric acid and gluconic acid through fermentation. Furthermore, a glucose oxidase (GOX) gene of the ZX033 strain is successfully knocked out through a genetic engineering means, and an engineering strain ZX033-delta GOX is constructed. The fumaric acid production capacity of the engineering strain is remarkably improved, compared with an original strain, the yield in a specific culture medium can be improved by 28.7% or above, and the engineering strain has better industrial application potential. The invention provides a new strain resource and a fermentation process for efficiently producing fumaric acid and gluconic acid.

The present application relates to the application of extracellular beta-glucan from Aureobasidium pullulans in preparing training immune inducer. By establishing mouse bone marrow derived macrophage (BMDMs) and zebrafish larvae training immune activation evaluation model, the immune activity of extracellular beta-glucan (eBG) from Aureobasidium pullulans is evaluated. It is found that compared with cell wall derived beta-glucan (cBG), the extracellular beta-glucan (eBG) from Aureobasidium pullulans has more significant training immune activation effect, which proves that the extracellular beta-glucan (eBG) from Aureobasidium pullulans is an excellent training immune inducer, and has good application prospect in the field of animal feed addition and disease immune prevention and control.

The application provides a method for preparing sialylated pullulan, which comprises the following steps: adding 1.0-30.0 g / L of sialic acid to a fermentation liquor of a strain of Aureobasidium pullulans EP1001 and / or a genetically engineered strain of Aureobasidium pullulans EP1001 after 24.0-60.0 hours of fermentation; catalyzing, by sialyltransferase, the transfer of sialic acid from an activated glycosyl donor to the end of a saccharide chain of a receptor pullulan to form a sialylated pullulan complex, i.e., sialylated pullulan; precipitating the supernatant of the centrifuged fermentation liquor by using an organic solvent; and finally obtaining the sialylated pullulan. The content of the pullulan in the sialylated pullulan is 55.05 g / L, the content of the sialylated pullulan in the sialylated pullulan is 9.58 g / L, the content of free sialic acid in the culture and / or the culture liquor is 0.42 g / L, and the sialic acid conversion rate is up to 95.8%.

The invention discloses an aureobasidium heat-resistant gene and application thereof, the nucleotide of the heat-resistant gene is shown as SEQ ID NO.1, and qPCR (quantitative polymerase chain reaction) analysis finds that the expression quantity of the gene at high temperature (35 DEG C) is increased by 1.25 times compared with that at proper temperature (28 DEG C). A recombinant expression cassette containing the gene and an overexpression vector are further constructed and are transformed into aureobasidium pullulans through agrobacterium tumefaciens mediation, and an overexpression strain OE-g0489 is obtained. A fermentation experiment shows that the malic acid yield, the biomass and the product yield of the overexpressed strain OE-g0489 are remarkably improved compared with those of an original strain at the high temperature of 35 DEG C. The invention proves that the g0489 gene has the function of enhancing the heat resistance of aureobasidium pullulans, and provides an important gene element and method for constructing high-temperature-resistant and high-yield industrial strains.

Based on the current situation that CSA is synthesized by microorganisms from the beginning in the prior art, the invention firstly provides application of a polycistron element P2A in construction of a chondroitin sulfate A expression cassette, the amino acid sequence of the polycistron element P2A is shown as SEQ ID NO: 1, and the polycistron element P2A is derived from porcine teschovirus type 1. The inventor finds that the polycistron element P2A can be successfully efficiently and independently expressed in the aureobasidium, an effective tool is provided for multi-gene collaborative expression, and the CSA is synthesized from the beginning in the aureobasidium strain. Based on the application of the polycistron element P2A, the invention also provides a chondroitin sulfate A expression cassette, an expression vector and an aureobasidium aureobasidium gene engineering strain for producing chondroitin sulfate through de novo synthesis. The aureobasidium pullulans engineering strain not only realizes de novo synthesis of chondroitin sulfate A, but also has significantly improved yield and sulfation degree compared with the prior art, and has good industrialization prospect and huge economic value.

The invention provides an anti-aging lifting and tightening traditional Chinese medicine mask and a preparation method thereof, and belongs to the technical field of skin care products. Comprising the following components in parts by weight: 2 to 8 parts of peach kernel extract, 2 to 8 parts of radix polygonati officinalis extract, 1 to 5 parts of radix asparagi extract, 0.1 to 1 part of sargassum parvifolium extract, 0.1 to 0.5 part of hydrolyzed pearl, 1 to 2 parts of carnosine, 0.5 to 1 part of ceramide NP liposome, 0.05 to 0.1 part of dipotassium glycyrrhizinate, 0.05 to 0.1 part of allantoin and 0.5 to 1 part of aureobasidium pullulans polysaccharide. The functions of the components are complementary, and the components cooperate with the physiological mechanism of the skin; the peach kernel extract improves microcirculation and resists oxidation; polygonatum odoratum polysaccharide has long-acting moisture retention; asparaginic acid promotes collagen synthesis; the sargassum parvifolium activates metabolism; the hydrolyzed pearls effectively promote barrier repair; ceramide NP supplements skin barrier components, carnosine, dipotassium glycyrrhizinate, allantoin and the like are supplemented, and the multiple effects of resisting oxidation, resisting saccharification, relieving and repairing are achieved.

The invention provides aureobasidium pullulans E1 and application of the aureobasidium pullulans E1 in co-production of pullulan and beta-glucan, and belongs to the technical field of biological fermentation. The aureobasidium pullulans E1 is preserved in the China General Microbiological Culture Collection Center on March 17, 2025, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.41836; the invention relates to an application of aureobasidium pullulans E1 in co-production of pullulan and beta-glucan. The strain aureobasidium pullulans E1 provided by the invention can co-produce pullulan and beta-glucan, and the yield of pullulan and beta-glucan is high.