72 results about "Phosphoenolpyruvate carboxykinase" patented technology

The invention relates to isolated nucleotide sequences from Coryneform bacteria which code for the pck gene encoding the enzyme phosphoenol pyruvate carboxykinase (PEP carboxykinase). The invention also relates a process for the fermentative preparation of L-amino acids, in particular L-lysine, L-threonine, and L-glutamate by attenuation of the pck gene.

The present invention relates to a recombinant adenovirus with increased in-vivo safety, tissue specificity, and anticancer activities, and a use thereof. Specifically, the recombinant adenovirus comprising: a promoter of the liver tissue-specific phosphoenolpyruvate carboxykinase (PEPCK) gene; a trans-splicing ribozyme which is operably linked to the promoter and acts on a cancer-specific gene; a therapeutic gene or a reporter gene which is linked to the 3′ exon of the ribozyme; and a serotype 35 fiber knob and a serotype 5 shaft, in which the orf4 gene is deleted from adenovirus E1, E3 and E4 orf1, shows remarkable in-vivo safety, high specificity for a target tissue, and remarkable anticancer effects, and thus can be useful for an anticancer drug or a cancer diagnostic agent as a gene delivery vector.

The invention discloses recombinant escherichia coli for efficiently producing succinic acid and a construction method thereof, and belongs to the technical field of bioengineering. The method comprises the following steps: knocking out a by-product encoding gene of a succinic acid producing strain E.coli FMME-N-2 to obtain a strain E.coli FMME-N-5(delta foc A-pflB-delta ldhA-delta pta-ackA); performing overexpression from actinobacillus succinogenes phosphoenolpyruvate carboxykinase pck and bacillus stutzeri phosphite dehydrogenase ptxD; introducing the constructed plasmid pTrcHisA-pck-ptxD into an expression host E.coli FMME-N-5(delta foc A-pflB-delta ldhA-delta pta-ackA), and screening through an ampicillin resistance flat plate to obtain an engineering bacterium E.coli FMME-N-5(delta foc A-pflB-delta ldhA-delta pta-ackA)-pck-ptx-D for efficiently producing succinic acid. The engineering strain adopts a two-stage fermentation strategy on a 7.5 L fermentation tank, the fermentation time is 96h, the succinic acid yield reaches 137g / L, the succinic acid yield reaches 1g / g glucose, the production strength is 1.43 g / L / h, byproducts lactic acid and formic acid are not accumulated, acetic acid is 1-2g / L, and the engineering strain has certain industrial production potential.

The present invention relates a method for transforming a C3 plant to provide it with a C4 photoshynthetic pathway by way of the introduction of some genes participating in a C4 photosynthetic pathway. To this end, the method of the invention comprises introducing a phosphoenolpyruvate carboxylase (PEPC) and a gene coding for a phosphoenolpyruvate carboxykinase (PCK) which has been connected with a DNA fragment coding for a transit peptide into a C3 plant.