7 results about "A serotype" patented technology

The invention belongs to the technical field of vaccine preparation, and particularly relates to a haemophilus parasuis adjuvant vaccine which comprises the following ingredients: serum type 4 haemophilus parasuis liquid, serum type 5 haemophilus parasuis liquid, an alumina gel adjuvant, a composite polysaccharide immunopotentiator, methanol, a solid culture medium and a liquid culture medium. According to the invention, the vaccine stock solution prepared by adding the mixed adjuvant and mixing serum type 4 and type 5 haemophilus parasuis solutions is inactivated and then inoculated to piglets, so that a larger antigen library is formed in the bodies of the piglets, the antigen library is slowly released in the application process, the action time of the antigen is prolonged, and meanwhile, the vaccine has a better promotion effect on the immune effect; the inoculation frequency is reduced, the drug resistance can be alleviated, the cost expenditure of farmers is reduced, and the morbidity and mortality of swinery are reduced.

Disclosed herein are methods for detecting the presence of a plurality of adeno-associated viruses (AAVs) or adenoviruses (Ad) in a sample. Methods for determining multiple serotypes of AAV or Ad virus in a sample are also described. Further disclosed are methods for detecting the presence of a heteroviral capsid protein in a sample comprising a serotype of AAV or Ad. Also disclosed is the use of LC-MS for detecting the presence of a plurality of AAVs or Ad in a sample; or a use for determining a plurality of serotypes of a plurality of AAVs or Ad in a sample; or to detect the presence of a heteroviral capsid protein in a sample comprising a serotype of AAV or Ad.

The invention relates to the technical field of molecular diagnosis, and discloses a specific primer for identifying a vibrio parahaemolyticus KUT1 type strain and a fluorescent PCR (Polymerase Chain Reaction) detection method. The invention provides a primer KUT1-F / R and a TaqMan probe for identifying a vibrio parahaemolyticus KUT1 type strain, and establishes a fluorescent PCR (Polymerase Chain Reaction) method (TaqMan probe method). According to the method, the KUT type of the main vibrio parahaemolyticus epidemic in diarrhea patients in China can be quickly identified, a serotyping system of the vibrio parahaemolyticus is expanded, and a more accurate tool is provided for epidemiological investigation and traceability. Compared with the prior art, the kit has the advantages that the due level of a fluorescent PCR technology is achieved in the aspects of amplification efficiency, sensitivity and specificity, so that efficient and specific detection on the KUT strain is realized, whether the strain is the mainly popular KUT type in China or not can be judged within one hour, and the actual use detection requirement is fully met.

This invention provides a serotyping method, a serotyping PCR kit, and a primer pair for Mannhemia haemolitica, enabling simple and rapid serotyping of the species. [Solution] For the 12 serotypes of Mannhemia haemolitica (A1, A2, A5, A6, A7, A8, A9, A12, A13, A14, A16, A17), 11 specific capsule-coding genes are targeted, with serotypes A14 and A16 having homologous capsule-coding genes.

The invention relates to the technical field of molecular diagnosis, and discloses a specific primer for identifying a vibrio parahaemolyticus KUT2 type strain and a fluorescent PCR (Polymerase Chain Reaction) detection method. The invention provides a primer KUT2-F / R and a TaqMan probe for identifying a vibrio parahaemolyticus KUT2 type strain, and establishes a fluorescent PCR (Polymerase Chain Reaction) method (TaqMan probe method). According to the method, the KUT type of the main vibrio parahaemolyticus epidemic in diarrhea patients in China can be quickly identified, a serotyping system of the vibrio parahaemolyticus is expanded, and a more accurate tool is provided for epidemiological investigation and traceability. Compared with the prior art, the kit has the advantages that the due level of a fluorescent PCR technology is achieved in the aspects of amplification efficiency, sensitivity and specificity, so that efficient and specific detection on the KUT strain is realized, whether the strain is the mainly popular KUT type in China or not can be judged within one hour, and the actual use detection requirement is fully met.

The present invention relates to novel immunogenic compositions comprising conjugated capsular saccharide antigens of Streptococcus pneumoniae (glycoconjugates) and uses thereof. The immunogenic compositions of the invention will generally comprise at least one saccharide conjugate from a serotype of Streptococcus pneumoniae that is not found in Prevnar, Synflorix and / or Prevnar 13. The invention also relates to the use of the novel immunogenic compositions for vaccinating human subjects, particularly infants and the elderly, against pneumococcal infection.

The present invention relates to the field of chemical synthesis, and disclosed is a chemical synthesis method for a serotype O5 O-antigen oligosaccharide of pseudomonas aeruginosa. In the present invention, a D-glucuronic acid building block and a D-fucosamine building block are used to construct an O-antigen trisaccharide, wherein the stereoselective synthesis of a D-fucosamine 1,2-α-cis-glycosidic bond relies on remote acyl participation and reagent regulation, and the synthesis of two 2,3-diaminomannuronic acid 1,2-β-trans glycosidic bonds is realized by means of SN2 nucleophilic substitution of a C2 azido group; and by means of selective assembly of protecting groups, orthogonal modification of modifying groups, and regulation of the reactivity of glycosyl donors and acceptors, the preparation of a multifunctionally modified O-antigen target trisaccharide is successfully completed. The method of the present invention is characterized in that raw materials are cheap and easily available and the preparation method is simple and easy to repeat, and has good prospects of application in the development of vaccines against pseudomonas aeruginosa.