Detection card for serotype O foot and mouth disease virus antibody and preparation method of detection card

Abstract

The invention discloses a detection card for a serotype O foot and mouth disease virus antibody and a preparation method of the detection card. The detection card comprises a detection card body assembled in a shell. The detection card body comprises a base plate. A sample pad, a marking pad, a nitrocellulose membrane and water absorption paper are sequentially arranged on the base plate. A serotype O foot and mouth disease antigen coats the nitrocellulose membrane to serve as a detection line, and an anti-chicken antibody produced in goat coats the nitrocellulose membrane to serve as a quality control line. A sample adding hole corresponding to the sample pad is formed in the shell. Window holes corresponding to the detection line and the quality control line on the nitrocellulose membrane are formed in the shell. The detection card is high in sensitivity, small in detection error in a batch and detection error between batches, good in repeatability, stable in performance, capable of fast detecting the serotype O foot and mouth disease virus antibody on site, and high in practical value.

Description

technical field [0001] The invention relates to a detection card, in particular to a detection card for rapid and quantitative detection of O-type foot-and-mouth disease virus antibody in pig serum and a preparation method thereof. Background technique [0002] Foot-and-Mouth Disease (Foot-and-MouthDisease, FMD) is caused by Foot-and-MouthDiseaseVirus (Foot-and-MouthDiseaseVirus, FMDV), an acute, febrile, highly contagious The disease is characterized by the occurrence of blisters on the mouth, tongue, lips, nose, hooves, breasts, etc., and ulceration and itching to form rotten spots. The disease has many transmission routes, fast speed, wide epidemic area, and can cause huge political and economic losses. The World Organization for Animal Health (Office International des Epizooties, OIE) listed it as a class A animal disease. The Chinese government also ranks it first among 14 first-class animal infectious diseases. At present, FMD is prevalent in most OIE member countrie...

AI Technical Summary

Problems solved by technology

[0006] Similarly, enzyme-labeled kits for detecting O-type foot-and-mouth disease antibodies are also widely used in the market, that is, liquid-phase blocking ELISA enzyme-labeled kits. ELISA kits require a microplate reader, incubation reaction conditions, plate washing conditions, operating environment and professional skills. In addition, the detection of samples also requires complex pre-treatment, such as blood collection, serum separation, etc.; and although rapid colloidal gold detection is convenient and does not require professional technicians and working environments, its detection sensitivity is low, and it can only be used qualitatively. narrow range

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