84results about How to "Small difference between batches" patented technology

The invention relates to the field of the clinic immunological detection, and in particular to a time resolution immunochromatographic test strip for quantitatively detecting pepsinogen I. The test strip comprises a plastic snap shell, a soleplate as well as a sample pad, a conjugate pad, a coating film and a piece of water absorption paper, which are sequentially adhered onto the soleplate in a staggering manner. The conjugate pad is coated with a pepsinogen I monoclonal antibody I marked by rare-earth ion microspheres; the coating film is coated with a detection band and a quality control band, a pepsinogen I monoclonal antibody II for recognizing different epitope is fixed on the detection band, and a rabbit anti-mouse IgG antibody is fixed on the quality control band. The invention also discloses a preparation method of the test strip. The two-antibody antigen sandwiched measuring technology and a time resolution immunochromatograhic technology are introduced to the detection of the pepsinogen I, and the single-person quantitative detection of the pepsinogen I is realized by combining a fluorescent detector; moreover, the sensitivity is high, the intra difference and inter difference are small, and great convenience is provided for the clinical use.

The invention discloses a fluorescent quantitative test paper strip for simultaneously detecting algal toxins MC-LR/RR/YR in a water body and a preparation method and application of the fluorescent quantitative test paper strip. The preparation method comprises the following steps: firstly, marking specific antibodies and rabbit IgG of three algal toxins by using fluorescent microspheres, and drying the three algal toxins on a fluorescent combining pad; sequentially drawing detection lines T1, T2 and T3 of conjugate of the three algal toxins and bovine serum protein and a goat anti-rabbit quality control line C on an NC membrane; finally assembling the test paper strip, namely, sequentially lapping and adhering a sample pad, a fluorescent marker conjugate pad, a nitrocellulose membrane with the three detection lines (T1, T2 and T3 lines) and one quality control line (line C), and a piece of water absorbing paper to a PVC hard board, after the components are assembled, shearing so as to obtain the test paper strip, and finally packaging the test paper strip in a plastic shell. The detection sensitivity on the three algal toxins, of the test paper strip disclosed by the invention, is up to 0.05ng/ml, the quantitative linear range of the test paper strip is 0.1-10ng/ml, the sample needs no pretreatment, the detection time is only 6 minutes, and rapid quantitative detection on the three common algal toxins in water bodies can be achieved, so that the test paper strip has very high practical values.

The invention relates to a process for preparing a veterinary rabies inactivated vaccine, in particular to a process for preparing a veterinary rabies inactivated and freeze-dried vaccine through a suspension culture cell. The method has the key point that: a bioreactor is used for large-scale suspension culture of baby hamster kidney BHK21-C13 cells and rabies virus is inoculated, and the rabiesvirus is subjected to mass propagation through a fed-batch and perfusion technology, so that a high-concentration and high-titer rabies antigen is obtained and concentrated, inactivated and purified to prepare the veterinary rabies inactivated vaccine; and thus, technical problems such as complexity, low antigen content, low effectiveness, large dosage, poor batch-to-batch variation and the like existing in the conventional process are solved.

The invention discloses a liquid ready-to-use prothrombin time detection reagent, which comprises buffer, synthetic phospholipid, recombinant rabbit tissue factor, surfactant and stabilizer. The synthetic phospholipid consists of phosphatidylserine, phosphatidylcholine and Cholesterol composition. The invention utilizes rabbit recombinant factors and synthetic phospholipids, selects the components of the synthetic phospholipids, and optimizes the stabilizer to prepare prothrombin time detection reagents, which can be used immediately after opening the bottle without reconstitution. The reagent overcomes the problem that the batch-to-batch difference of existing prothrombin time detection reagents is difficult to control, and has high sensitivity, good stability, small batch-to-batch difference, easy quality control, and easy production.

The invention discloses a detection card for a serotype O foot and mouth disease virus antibody and a preparation method of the detection card. The detection card comprises a detection card body assembled in a shell. The detection card body comprises a base plate. A sample pad, a marking pad, a nitrocellulose membrane and water absorption paper are sequentially arranged on the base plate. A serotype O foot and mouth disease antigen coats the nitrocellulose membrane to serve as a detection line, and an anti-chicken antibody produced in goat coats the nitrocellulose membrane to serve as a quality control line. A sample adding hole corresponding to the sample pad is formed in the shell. Window holes corresponding to the detection line and the quality control line on the nitrocellulose membrane are formed in the shell. The detection card is high in sensitivity, small in detection error in a batch and detection error between batches, good in repeatability, stable in performance, capable of fast detecting the serotype O foot and mouth disease virus antibody on site, and high in practical value.

The invention belongs to the field of biotechnology and relates to the purification technology for avian influenza viruses, which is applicable to the mass production of human-used avian influenza vaccines. The purification technology is characterized in that: the avian influenza viruses are inoculated on a 9 to 11 days chicken embryo; the influenza viruses are cultured for 68 to 72 hours; and the allantoic fluid of the chicken embryo is sequentially collected, coarsely filtered, centrifugated with a continuous flow, ultrafiltered, condensed, centrifugated in a sucrose density gradient to be purified. The technology has the advantages of simple operation, small difference between batches, stable quality, high yield, low ovalbumin content and the like.

The invention discloses a porcine reproductive and respiratory syndrome antibody detecting card and a preparation method thereof. The porcine reproductive and respiratory syndrome antibody detecting card comprises a detecting card body assembled in a shell, wherein the detecting card body comprises a bottom plate; a sample pad, a label pad, a nitrocellulose film and absorbent paper are sequentially arranged on the bottom plate; the nitrocellulose film is coated with porcine reproductive and respiratory syndrome antigens serving as a detection line, and coated with goat anti-chicken antibodies serving as a quality control line; a sample adding hole is formed in the shell and corresponds to the sample pad; and window holes are formed and correspond to the detection line and the quality control line on the nitrocellulose film. The porcine reproductive and respiratory syndrome antibody detecting card is high in sensitivity, small in detection intra-batch difference and inter-batch difference, good in repeatability and stable in performance, can quickly detect porcine reproductive and respiratory syndrome antibodies in situ, and has high practical value.

The invention discloses a detection card for quickly quantitative detection of echinococcosis antibody in serum. The detection card includes a detection card case and a test paper strip assembled therein. The test paper strip includes a plastic base board provided with pressure-sensitive adhesive, wherein a sample pad, a marker pad, a nitrocellulose membrane and water absorption paper are successively adhered to the base board. The marker pad is composed of a carrier base layer and a marker. The marker is a film formed by spray-coating the carrier base layer with lanthanum-series fluorescencedetection microspheres and lanthanum-series fluorescence quality control microspheres. The nitrocellulose membrane is coated with an echinococcosis recombinant antigen to form a detection line and rabbit-anti-chicken IgY antibody to form a quality control line. The marker includes the fluorescence detection microspheres labeled by the echinococcosis recombinant antigen and the fluorescence qualitycontrol microspheres labeled by the chicken IgY antibody. The detection card can achieve quick quantitative detection of the echinococcosis antibody in situ and has great practical and application values.

The invention relates to a test strip capable of quantificationally detecting content of c-reactive protein in peripheral blood. The test strip comprises a sample cushion, a quantum cushion, a nitrocellulose membrane, water absorbing paper and a lining plate, the quantum cushion wraps c-reactive protein antibodies marked by carbon quantum dots, and a detecting line and a quality control line are successively arranged on the nitrocellulose membrane from the sample cushion side to the water absorbing paper side; the detecting line wraps c-reactive protein recognizing monoclonal antibodies, and the quality control line wraps goat anti mouse IgG. The test strip is simple in structure and can quickly and accurately detect CRP in the peripheral blood quantificationally, the detection of the teststrip is high in sensitivity and stability and good in specificity, the detecting time is shorter than 15 min, and the diagnostic efficiency and the work efficiency of detecting operators can be greatly improved.

The invention discloses a needle milling production process of a powdery cell culture medium. The process includes the steps of: 1) weighing raw materials according to types for later use; 2) pre-mixing the weighed raw materials in the step 1); 3) grinding and pulverizing the pre-mixed raw materials by means of a needle-type grinding miller; 4) final-mixing the pulverized materials in the step 3); and 5) packaging the final-mixed product. The process can achieve continuous production of the cell culture medium, wherein the productivity of the process is equal to that of a process with a large-size ball miller. The process is less in occupied area of equipment, saves production cost and production time, is high in productivity, is less in batch difference and has stable product quality.

The invention discloses a quick quantitative determination card for a canine parvovirus antibody and a use method thereof. The quick quantitative determination card comprises a detection card casing and a test strip assembled in the detection card casing, wherein the test strip comprises a plastic bottom plate with pressure sensitive adhesive, and a sample pad, a marker pad, a nitrocellulose membrane and absorbent paper are sequentially bonded to the bottom plate; the marker pad comprises a carrier base layer and a marker; the marker is a layer of membrane which is formed by spraying lanthanide-series fluorescence detection microspheres and lanthanide-series fluorescence quality control microspheres on the carrier base layer; the nitrocellulose membrane shows a detection line when being coated with a canine parvovirus recombinant antigen and shows a quality control line when being coated with a rabbit anti-chicken lgY antibody; the marker is fluorescence detection microspheres marked with a canine parvovirus structural protein VP2 recombinant antigen and fluorescence quality control microspheres marked with a chicken lgY antibody. The quick quantitative determination card disclosedby the invention can achieve in-site quick quantitative detection on the canine parvovirus antigen and has higher practical value and popularization value.

The invention relates to an aptamer percolated biochip and a preparation method thereof. The preparation method comprises the following steps: arranging a window on a casing of the biochip; arranging a nitrocellulose membrane, a reverse osmosis layer, a water absorbing layer and a leakage preventing layer in the casing of the biochip from top to bottom; fixing an aptamer molecule for a target substance on the surface of the nitrocellulose membrane, wherein the aptamer molecule is RNA (Ribose Nucleic Acid), DNA (Deoxyribose Nucleic Acid) or modified RNA or DNA. According to the aptamer percolated biochip and the preparation method thereof disclosed by the invention, the defects of limited detection range, low sensitivity, weak specificity, easiness for deterioration, complex process, long reaction time and the like existing in the prior art are overcome; DNA molecules which are specifically combined with target molecules are screened from mass in-vitro single stranded oligonucleotide libraries through SELEX screening and are amplified greatly to obtain an aptamer; in addition, a sample to be detected can quickly pass through a micropore of the nitrocellulose membrane by the percolated biochip; the target molecule of the percolated biochip can be quickly combined with the aptamer on the membrane, so that the reaction time can be greatly shortened; higher sensitivity and specificity are obtained.

The invention provides a heterogenous conjugate. The heterogenous conjugate comprises a tetramer shaped heterogenous crosslinking antibody formed by mouse anti-RV (rubella virus) McAb and healthy human IgG (immunoglobulin G) which is not infected with RV, wherein the antibody can serve as a substitute of an RV antibody in the positive blood of patients and can be used in immunoassay kits as a quality control product replacing the positive blood of patients after being diluted in a diluent. The heterogenous conjugate is simple to produce and prepare, is low in cost, has very good safety and stability at the same time and can conduce to reducing the assay errors.

The invention provides a reagent or a kit for magnetic particle chemiluminescence detection of salivary liquefied carbohydrate chain antigen and application of the reagent or the kit, and belongs to the technical field of in-vitro diagnostic reagents. The reagent comprises a magnetic particle reagent and an acridinium ester reagent, wherein the magnetic particle reagent is composed of 2-8 [mu] g/ml of a magnetic particle labeled KL-6 antibody 1, 20%-30% of newborn bovine serum, 0.01%-0.1% of a preservative, 20%-30% of glycerin, 0.01%-0.1% of Tween 20, 0.1%-1% of BSA and 50%-70% of a PBS buffer solution, and the acridinium ester reagent is composed of 6-10 [mu] g/ml of an acridinium ester labeled KL-6 antibody 2, 10%-40% of glycerin, 0.5%-5% of BSA and 60%-90% of a PBS buffer solution. The KL-6 detection kit has the characteristics of high sensitivity, high precision and high accuracy, has excellent anti-interference capability, and does not have an obvious HOOK effect under a high-concentration sample of 40000IU/mL.

The invention relates to a plate-type chemiluminescence-method detection kit for a carbohydrate antigen 50. Agents of the kit include a biotin-marked CA50 antibody solution, an enzyme linked immunosorbent assay plate coated with an antibiotin antibody, a CA50 antibody solution marked by horseradish peroxidase, CA50 calibrating materials A-F, a concentrated washing solution, a substrate liquid A and a substrate liquid B. According to a main design thought of the kit, an antibiotin antibody-biotin system is introduced based on chemiluminescence immune assay, detection specificity of the kit is stabilized, detection sensitivity is greatly increased and detection time is reduced. A high-temperature oscillating type antibiotin antibody coating process is improved, thus reducing the using amount of the antibody/antigen, simplifying agent production procedures and shortening the production period.

The invention discloses a large-scale production method for veterinary pseudorabies attenuated live vaccines. The method comprises the steps of culturing pseudorabies virus attenuated strains by using a cell suspension process so that the density of BHK21-C13 cells subjected to suspension culture in a reactor is more than or equal to 2*10<6> cells per milliliter, then culturing with a virus maintenance solution containing pseudorabies suspended virus seeds, and performing clarification, seedling, packaging and freeze-drying on the harvested pseudorabies attenuated virus solution to obtain the veterinary pseudorabies attenuated live vaccines. By adopting the method, mass multiplication of pseudorabies attenuated viruses can be realized, the titer of viruses and the yield of antigen are greatly improved, process automation is realized, the quality of the vaccines is improved, the density of the BHK21-C13 cells can be improved, the optimal biochemical condition of cell growth or virus multiplication can be automatically monitored, the titer of viruses is improved, large-scale production of the process is relatively easy, and the vaccines are stable in quality and low in batch difference and have broad production and application prospect.

The invention discloses a time-resolved fluorescence immunochromatographic test strip and kit for detection of cTnI, and preparation methods thereof. The test strip comprises a bottom liner and further comprises a sample pad, a conjugate pad, a coating membrane and absorbent paper successively arranged on the bottom liner, wherein the conjugate pad is coated with a cTnI monoclonal detection antibody labeled by fluorescence microspheres; the coating membrane comprises a test zone and a control zone arranged in parallel and spaced apart from each other; the test zone is coated with a cTnI monoclonal capture antibody capable of recognizing a single epitope, and the control zone is coated with a goat anti-mouse IgG antibody; and the coating membrane a nitrocellulose membrane bonded with a polymer, and the polymer is a material having light transmittance of 10% or below under irradiation of light with wavelengths of less than 450 nm and light transmittance of 95% or more under irradiation of light with wavelengths of 500 nm or more. The test strip of the invention can realize quick and quantitative detection, and is accurate and reliable in test results and high in sensitivity; the preparation method is simple in process and suitable for large-scale production; and the test strip has positive significance to quantitative detection of cTnI.

The invention discloses a rabies virus rapid antibody quantitative detection card and an application method thereof. The rabies virus rapid antibody quantitative detection card comprises a detection card housing and a test strip assembled in the detection card housing, wherein the test strip comprises a plastic baseplate with a pressure-sensitive adhesive; a sample pad, a marker pad, a nitrocellulose membrane and absorbent paper are sequentially bonded on the baseplate; the marker pad consists of a carrier base layer and a marker; the marker is a membrane formed by spraying lanthanide fluorescence detection microspheres and lanthanide fluorescence quality control microspheres onto the carrier base layer; the nitrocellulose membrane is coated with a rabies virus recombinant antigen as a detection line and coated with a rabbit anti-chicken IgY antigen as a quality control line; and the marker is fluorescence detection microspheres marked with a rabies virus structural protein G recombinant antigen and fluorescence quality control microspheres marked with a chicken IgY antibody. The rabies virus rapid antibody quantitative detection card can realize field rapid quantitative detectionof a rabies virus antibody and has a high practical value and popularization value.