A plate-type chemiluminescence-method detection kit for a carbohydrate antigen 50 and a preparing method

A chemiluminescence method and a detection kit technology, which are applied in the direction of chemiluminescence/bioluminescence, biological testing, and analysis through chemical reactions of materials, etc., can solve the basis for early diagnosis or diagnosis of malignant tumors, and are not suitable for the general population Insufficient tumor screening, sensitivity and anti-interference ability, etc., to achieve the effect of shortening production time, reducing usage, and improving sensitivity

Inactive Publication Date: 2017-12-19
JIANGSU FLON BIOTECH
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Problems solved by technology

In the past, human CA50 antigen assay kits represented by enzyme-linked immunoimmunoassay technology were seriously insufficient in sensitivity and anti-interference ability due to methodological limitations. Most of them are ELISA, time-resolved fluorescent immunoassay and chemiluminescence technology, among which chemiluminescence technology emerged in the 1980s and is an emerging technology developed after ELISA and radioimmunoassay. High sensitivity, high specificity

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  • A plate-type chemiluminescence-method detection kit for a carbohydrate antigen 50 and a preparing method
  • A plate-type chemiluminescence-method detection kit for a carbohydrate antigen 50 and a preparing method
  • A plate-type chemiluminescence-method detection kit for a carbohydrate antigen 50 and a preparing method

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Example Embodiment

[0073] The present invention will be described below in conjunction with specific examples. The given examples are only general illustrations of the products or methods of the present invention, which help to better understand the present invention, but do not limit the scope of the present invention. The experimental methods described in the following examples, unless otherwise specified, are conventional methods; the materials, unless otherwise specified, can be obtained from commercial sources.

[0074] The main reagents of the plate type chemiluminescence detection kit for carbohydrate antigen 50 in this embodiment include: the included reagents are biotin-labeled CA50 antibody solution, anti-biotin antibody-coated microtiter plate, horseradish peroxidase-labeled CA50 antibody solution, CA50 calibrator A-F, concentrated washing solution, substrate solution A, substrate solution B.

[0075] Preparation method of the kit

[0076] First, the preparation of concentrated lotio...

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Abstract

The invention relates to a plate-type chemiluminescence-method detection kit for a carbohydrate antigen 50. Agents of the kit include a biotin-marked CA50 antibody solution, an enzyme linked immunosorbent assay plate coated with an antibiotin antibody, a CA50 antibody solution marked by horseradish peroxidase, CA50 calibrating materials A-F, a concentrated washing solution, a substrate liquid A and a substrate liquid B. According to a main design thought of the kit, an antibiotin antibody-biotin system is introduced based on chemiluminescence immune assay, detection specificity of the kit is stabilized, detection sensitivity is greatly increased and detection time is reduced. A high-temperature oscillating type antibiotin antibody coating process is improved, thus reducing the using amount of the antibody/antigen, simplifying agent production procedures and shortening the production period.

Description

technical field [0001] The invention belongs to the field of biological kit materials, and in particular relates to a quantitative detection kit and detection method for carbohydrate antigen 50, which can quickly, simply, efficiently and sensitively quantitatively detect carbohydrate antigen 50 in human serum samples. Background technique [0002] CA50 (Carbohydrate Antigen 50, referred to as CA50) refers to a tumor marker (Tumor Marker), which is a chemical substance that reflects the existence of a tumor. They either do not exist in normal adult tissues but are only found in embryonic tissues, or their content in tumor tissues greatly exceeds that in normal tissues. Their existence or quantitative changes can indicate the nature of tumors, so as to understand the histogenesis and cell differentiation of tumors , cell function, to help tumor diagnosis, classification, prognosis judgment and treatment guidance. [0003] CA50 is a sialic acid ester and sialic acid glycoprote...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574G01N21/76
Inventor 钱晓锦彭会军戴宝平张伟
Owner JIANGSU FLON BIOTECH
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