Fluorescent quantitative test paper strip for simultaneously detecting algal toxins MC-LR/RR/YR and preparation method and application of fluorescent quantitative test paper strip

Abstract

The invention discloses a fluorescent quantitative test paper strip for simultaneously detecting algal toxins MC-LR/RR/YR in a water body and a preparation method and application of the fluorescent quantitative test paper strip. The preparation method comprises the following steps: firstly, marking specific antibodies and rabbit IgG of three algal toxins by using fluorescent microspheres, and drying the three algal toxins on a fluorescent combining pad; sequentially drawing detection lines T1, T2 and T3 of conjugate of the three algal toxins and bovine serum protein and a goat anti-rabbit quality control line C on an NC membrane; finally assembling the test paper strip, namely, sequentially lapping and adhering a sample pad, a fluorescent marker conjugate pad, a nitrocellulose membrane with the three detection lines (T1, T2 and T3 lines) and one quality control line (line C), and a piece of water absorbing paper to a PVC hard board, after the components are assembled, shearing so as to obtain the test paper strip, and finally packaging the test paper strip in a plastic shell. The detection sensitivity on the three algal toxins, of the test paper strip disclosed by the invention, is up to 0.05ng/ml, the quantitative linear range of the test paper strip is 0.1-10ng/ml, the sample needs no pretreatment, the detection time is only 6 minutes, and rapid quantitative detection on the three common algal toxins in water bodies can be achieved, so that the test paper strip has very high practical values.

Description

technical field [0001] The invention belongs to the technical field of algae toxin detection, and in particular relates to a fluorescent immunochromatographic test strip capable of simultaneously performing rapid and quantitative detection of algae toxins MC-LR / RR / YR in a water body, and a preparation method and application thereof. Background technique [0002] With the aggravation of environmental pollution and eutrophication, harmful algal blooms, especially cyanobacterial blooms, frequently occur in freshwater basins, and have become an environmental problem of widespread concern at home and abroad. Many cyanobacteria can produce toxins represented by microcystin MC, and more than 60 isomers have been found so far. It is a type of hepatotoxin with strong cancer-promoting effects, among which the most common and abundant There are three types of LR, RR, and YR. [0003] [0004] Since fresh water is the drinking water source for humans and animals, microcystins caused...

AI Technical Summary

Problems solved by technology

Chromatography can carry out qualitative and quantitative analysis and determination of microcystins, and the results are reliable. However, the instruments used in chromatography are expensive and require professional personnel to operate. The pretreatment is complicated, the analysis period is long, and the cost is high. Generally, only Suitable for confirmatory testing

The protein phosphatase inhibition method is not yet mature enough. Many variables in the enzyme reaction system have not been quantified and unified standards have not been established, and the selectivity is poor. Many substances in the environment can interfere with the determination, and the results are not accurate enough. Therefore, practical applications are limited. very restrictive

Immunoassay is an analysis method based on antigen-antibody specific reaction, which has the characteristics of strong specificity, high sensitivity, fast detection speed, and low cost. It is currently a commonly used routine detection method, including enzyme-linked immunosorbent assay (ELISA) , colloidal gold rapid detection method, etc., but they all have their own advantages and disadvantages. Although the enzyme-linked immunosorbent assay has high detection sensitivity and accurate results, the operation is relatively complicated, the time is long, and only one toxin can be detected at a time; colloidal gold The method does not require pretreatment of the sample, and the detection is fast, but the sensitivity is relatively low, and only qualitative or semi-quantitative detection can be achieved, and quantitative detection results cannot be given.

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