Large-scale production method for veterinary pseudorabies attenuated live vaccines

Abstract

The invention discloses a large-scale production method for veterinary pseudorabies attenuated live vaccines. The method comprises the steps of culturing pseudorabies virus attenuated strains by using a cell suspension process so that the density of BHK21-C13 cells subjected to suspension culture in a reactor is more than or equal to 2*10<6> cells per milliliter, then culturing with a virus maintenance solution containing pseudorabies suspended virus seeds, and performing clarification, seedling, packaging and freeze-drying on the harvested pseudorabies attenuated virus solution to obtain the veterinary pseudorabies attenuated live vaccines. By adopting the method, mass multiplication of pseudorabies attenuated viruses can be realized, the titer of viruses and the yield of antigen are greatly improved, process automation is realized, the quality of the vaccines is improved, the density of the BHK21-C13 cells can be improved, the optimal biochemical condition of cell growth or virus multiplication can be automatically monitored, the titer of viruses is improved, large-scale production of the process is relatively easy, and the vaccines are stable in quality and low in batch difference and have broad production and application prospect.

Description

technical field [0001] The invention belongs to a preparation method of a vaccine in the field of biomedicine, in particular to a method for producing an attenuated live vaccine of pseudorabies for animals by using suspension culture cells. Background technique [0002] Pseudorabies is an acute infectious disease caused by a variety of domestic and wild animals caused by pseudorabies virus (PRV), especially one of the major infectious diseases that endanger the global swine industry. [0003] At present, pseudorabies is widespread in the world, and more than 40 countries and regions have reported the occurrence of pseudorabies. With the continuous development of large-scale pig farming and the emergence of virulent strains, the occurrence and prevalence of porcine pseudorabies are becoming more and more serious. Porcine pseudorabies has been reported in more than 20 provinces and cities in my country, and several PRV strains have been isolated successively. DQ-8401, S, Y st...

AI Technical Summary

Problems solved by technology

[0004] At present, the method of domestic production of pseudorabies vaccine still adopts the method of adherent culture of primary or passaged cell lines, which adopts the traditional spinner bottle production process, which has the advantages of small batches of antigens, large batch-to-batch differences, low efficiency, high labor intensity, and occupation. Disadvantages such as many venues

And the present invention utilizes suspension culture technology not only can overcome the shortcoming of above-mentioned spinner bottle production technology, but also can improve antigen output, save cost, improve product quality, but the suspension culture technology and its application of production pseudorabies attenuated live vaccine do not have at present Related reports

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