9results about How to "Solve output" patented technology

The invention discloses a cotton salt-tolerant breeding device and a breeding method thereof, and relates to the technical field of cotton salt-tolerant breeding, the cotton salt-tolerant breeding device comprises a base, the top of the base is fixedly connected with a support frame, the lower position of the surface of the support frame is fixedly connected with a hydraulic cylinder, and the bottom of the inner side surface of the support frame is slidably connected with a storage box. According to the cotton salt-tolerant breeding device and the breeding method thereof, saline water is sprayed into a storage box through a spray head, a telescopic cylinder drives a stirring assembly to move downwards, the stirring assembly extends into a substrate, the stirring assembly is driven by the telescopic cylinder to repeatedly ascend and descend to stir the substrate and the saline water, and the salinity and alkalinity of the substrate are increased; the linear guide rail drives the supporting plate to reset, the grabbing and conveying mechanism grabs the salinity matrix and then puts the salinity matrix into the cultivation mechanism, and the problem of how to select the cultivation salinity range of cotton seeds so as to improve the yield of cotton planted in the saline-alkali soil is solved.

The present invention discloses a method for producing stropharia by graded fermentation and a method for cultivating stropharia in rotation with celery. A method for producing stropharia by graded composting fermentation comprises the following steps: 1) mixing sawdust, lime and water to form mixture A, performing primary composting fermentation to obtain primary fermentation material; 2) mixing the primary fermentation material, cottonseed hulls, rice husks, bran, lime and water to form mixture B, performing secondary composting fermentation to obtain secondary fermentation material; 3) mixing the secondary fermentation material with water to form culture medium, sowing stropharia strains on the culture medium, performing mycelium culture, and covering with soil to form a covering layer; 4) performing fruiting culture to obtain stropharia. The method of the present application significantly shortens the time from sowing to fruiting and harvesting and significantly improves the average yield through graded composting fermentation, three-layer paving and covering with soil after the mycelium crawls to the surface of the paving.

This utility model relates to the field of water oxygenation equipment, and specifically discloses a water oxygenation and temperature regulation device. It includes a tank for water storage, an air inlet pipe, and an air outlet pipe. The air inlet end of the air inlet pipe is connected to a blower. The tank is a closed structure, and there are multiple tanks, all located below ground level. Adjacent tanks are connected by air guide pipes. The air inlet end of the air guide pipe is connected to the tank upstream of the gas transmission path and is located above the water level inside that tank; the air outlet end is connected to the tank downstream of the gas transmission path and is located below the water level inside that tank. The air inlet pipe is installed on the tank at the beginning of the gas transmission path, with its bottom end below the water level inside that tank; the air outlet pipe is installed on the tank at the end of the gas transmission path, with its bottom end above the water level inside that tank. The water temperature in the tank can be automatically adjusted according to the underground temperature, solving the problem in the prior art where the water temperature is greatly affected by the external natural environment, leading to unstable water temperature.

The invention relates to the technical field of tea processing, in particular to eurotium cristatum Liupu tea and a stable eurotium cristatum growing process of loose tea thereof. The loose tea stable eurotium cristatum growing process of the golden flower Liupu tea comprises the following steps: sequentially carrying out rehydration, eurotium cristatum inoculation, loose pressing and shaping and mold fermentation on raw tea to obtain a golden flower Liupu tea finished product; wherein steaming is carried out before inoculation of the eurotium cristatum or / and after loose pressing and shaping, the moisture content of the tea leaves during loose pressing and shaping is 25-30%, and a kraft paper layer with the specification of 100-180g / m < 2 > is adopted on the inner wall of a loose pressing and shaping mold to wrap the tea leaves. Kraft paper with a specific thickness is adopted in a mold for wrapping and shaping, and appropriate tea moisture content in a fermentation stage is combined, so that stable eurotium cristatum growing is realized, and the problems of low eurotium cristatum content and non-uniform eurotium cristatum growing effect in the eurotium cristatum Liupu tea finished product are remarkably improved; meanwhile, the eurotium cristatum growing time of the loose tea is obviously shortened.

A strain of northern cold-region Morchella WZ-23 and a cultivation method of its fruiting body, the present invention relates to a strain of northern cold-region Morchella WZ-23 and a cultivation method of its fruiting body. The purpose of the present invention is to solve the problem of low yield of existing Morchella under low temperature conditions. The present invention is a strain of northern cold-region Morchella WZ-23, which is Morchella eohespera WZ-23 from three places, preserved in the China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan, China, the preservation date is December 5, 2023, and the preservation number is CCTCC NO: M 20232465. The strain screened by the present invention solves the problem of unstable yield and traits in the northern cold region because the main cultivated varieties are southern varieties. The present invention is applied to the field of edible fungi.

The invention discloses a method and a device for creating heat-resistant potato germplasm through interspecific grafting. The method comprises the following steps: (1) cultivating potato seedlings and tomato seedlings; (2) mutual stock-scion grafting; 3, grafted plant treatment, wherein after grafting is conducted for three weeks, grafting openings are pulled to the positions 15 cm below plate holes, and variable temperature treatment is conducted; (4) potato seed management: cutting off all the grown stolons before temperature-changing treatment, leaving the potatoes after temperature-changing treatment, and harvesting the potatoes at proper time; and (5) heat-resistant domestication: after 5-6 compound leaves grow on the seed potato potted plant in the step (4), performing high-temperature domestication at 38 DEG C in the daytime and 30 DEG C at night in an illumination incubator for 9 days, transferring the heat-resistant plant to outdoor culture, and harvesting tubers. And (6) heat-resistant germplasm screening: repeating the step (5) for two generations, and harvesting tubers. According to the method, the potato germplasm with high heat resistance and genetic stability is obtained, the variety breeding process is shortened, and the problems that a heat-resistant potato germplasm creation technology and heat-resistant varieties are lacked, and potato production is affected by high temperature are solved.

The present invention discloses a method for large-scale extraction of extracellular membrane vesicles (OMV / FGF2) secreted by Escherichia coli that has ampicillin resistance and can secrete bFGF protein after gene editing. The method cultured Escherichia coli in LB liquid containing 100ug / mL ampicillin, and used ultrasonic separation and filtration technology to effectively separate the extracellular membrane vesicles loaded with bFGF protein from Escherichia coli without Escherichia coli cells. The extraction method is simple and easy to operate, and the yield of extracellular membrane vesicles is greatly improved under the premise of retaining most of the effective biological components of bacterial outer membrane vesicles. At the same time, the method effectively removes the live bacterial cells of Escherichia coli, ensures the safety of the body, and greatly reduces the risk of bacteremia or scurvy induced by microorganisms entering the blood. In addition, the OMV / FGF2 extracted by the method was applied to colitis wounds, and it was found that it could significantly promote the healing of colitis wounds.

A sorghum cultivation method comprises the steps that firstly, the ridge spacing is set, the interval between every two adjacent ridges ranges from 40 cm to 60 cm, and the plant spacing is set and ranges from 20 cm to 30 cm; step 2, ploughing before sowing: carrying out first rough ploughing before sowing, and carrying out second fine ploughing in the sowing process; step 3, sowing: sowing according to the set plant spacing; and step 4, watering after sowing, and irrigating after sowing until the earth surface is completely wet. The row spacing and the ridge spacing are designed according to the local annual rainfall and the soil acidity and alkalinity, meanwhile, a two-time ploughing mode is adopted, soil breaking is conducted through first-time ploughing, hardened large-block soil is smashed, soil refining can be achieved through second-time ploughing, and the yield of the soil is increased. Therefore, the occurrence of soil hardening in the whole growth period of the sorghum can be reduced to the greatest extent, and the problem that the yield of the sorghum is affected due to surface hardening when the sorghum is planted in marginal land is solved.