92 results about "Sporocarp (fungi)" patented technology

The invention discloses a hericium erinaceus cultivation method comprising the steps of culture material preparation, fungi bag preparation, sterilization, inoculation, greenhouse construction, mycelium culturing, fruiting management, harvesting, and post management. The cultivation process provided by the invention is simple, and the cost is low. The cultivated hericium erinaceus has the advantages of fast mycelium germination, robust growth, uniform and neat primordium, ordered fruiting body growth condition, robust fruiting body growth, and high biological efficiency.

The invention belongs to the field of microbial fungi, and relates to a selenium-rich cordyceps militaris strain CM001; the wild cordyceps militaris strain which is newly separated in the nature is selected as an original strain; the original strain is subjected to ultraviolet mutation, and the strain can be obtained by separating and screening; the selenium-rich cordyceps militaris strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 18, 2014 with the preservation registration number of CGMCC No.9460, is named as CM001 and is named as cordyceps militaris in a classified way. Under the artificial culture conditions, the cordyceps militaris strain has the tolerance for high-concentration selenium, and can carry out biotransformation in a body; the hyphae yeast, the growth matrix or the sporocarp of the selenium-rich cordyceps militaris strain can be used as raw materials of medicines, foods and drinks, or can be prepared into powder, tablets, freeze-drying powder, capsules or electuary.

The invention provides a living Chinese caterpillar fungi artificially cultivated by using larvae as hosts, which comprise insect larvae and one or two about two centimeter living Chinese caterpillar fungus fruiting bodies grown on the head of each the larva. The cultivating process comprises the steps of: invasion of the Chinese caterpillar fungi into the living insect larvae, in vivo growth of the hyphae in the living insect larvae, inactivation of the larvae, growth of the Chinese caterpillar fungus fruiting bodies on the heads and the like; and in the whole process, not only the insect larvae and the hyphae are grown in the polypide, but also the Chinese caterpillar fungus fruiting bodies are grown on the heads, the obtained Chinese caterpillar fungi have the same appearance and almost the same nutrient content as the wild Chinese caterpillar fungi, so that the Chinese caterpillar fungi obtained by the method are the real living Chinese caterpillar fungi obtained by artificial cultivation.

The invention relates to a method for separating polysaccharide and protein by using a choline ionic liquid two-phase aqueous system, belongs to the technical field of bioactive substance separation and purification, and particularly relates to application of the ionic liquid two-phase aqueous system composed of choline chloride [Ch]Cl and inorganic phosphate K3PO4 in selective separation of fungal polysaccharide and protein. The method is mainly characterized by comprising the following steps: after degreasing crushed fungi mycelium or sporocarp with petroleum ether, using hot water with the temperature of 95 DEG C for reflux extraction for 8 hours, centrifuging, concentrating, dialyzing, and freeze-drying, so as to obtain a fungus aqueous extract; carrying out extraction separation by using the ionic liquid two-phase aqueous system composed of choline chloride[Ch]C1 and inorganic phosphate K3P04; carrying out lower-layer inorganic salt phase dialysis, ethanol precipitation and freeze-drying so as to obtain fungal polysaccharide; carrying out extraction on an upper-layer ionic liquid phase by using dichloromethane, concentrating and recycling an ionic liquid. The ionic liquid two-phase aqueous system provided by the invention is suitable for industrial production of active polysaccharides, has the effects of being green, quick and efficient, the ionic liquid can be recycled for use, the production cost is lowered, and the ionic liquid two-phase aqueous system can be commonly used for the selective separation of various fungal polysaccharide and protein.

The invention relates to a strain preparation method of rare wild edible mushroom termitomyces robustus and belongs to the technical field of macrofungi culture. The preparation method is characterized in that sporocarp which is not opened at the cap is used as a material, and solid cultivated strain is prepared by using ways such as mycelium induction, mycelium amplication culture and rapid propagation. According to the invention, termitomyces robustus mycelia is special extremely slow in growing and difficult to propagate on a solid culture medium, and is difficult to grow on a cultivation medium, batch strains are difficultly produced, and the condition is one of factors restricting or influencing artificial culture. According to the invention, the termitomyces robustus mainly producedin Yunnan is taken for an example, and a creative break is generated for the solid culture technology of the mycelia, so that the mycelia can rapidly grow and propagate, culture period is greatly shortened, and bagged cultivated strain is produced. In the preparation method provided by the invention, mother strain, amplification and cultivation culture mediums are used, and are simple and available in components, low in cost and convenient for popularization, such as cultivated strain culture medium mainly makes use of primitive environment soil and wheat bran, which provides conditions for artificial domestication and cultivation.

The invention discloses a method for preparing artificially domesticated original strains of wild red-soil termitomyces albuminosus, which is the practical application of an artificial domestication biotechnology, and solves the technical problem of artificial cultivation of wild fungi. The technical method comprises the following steps: (1) collecting entities of wild red-soil termitomyces albuminosus naturally grown on high plateau areas (altitude: more than 1800 meters) in Yunnan Province so as to obtain spores; (2) carrying out screening on the spores by using a single spore hybridization technique so as to obtain fruiting fungus strains of red-soil termitomyces albuminosus; and (3) carrying out systematic seed selection through sporocarp tissue separation, after 2-6 years of systematic cultivation, realizing the artificial domestication of red-soil termitomyces albuminosus, so that the original strains of wild red-soil termitomyces albuminosus, which are strong in stability and can be used for breeding production are obtained. The original strains of wild red-soil termitomyces albuminosus disclosed by the invention can be used for artificially planting and producing red-soil termitomyces albuminosus.

The invention discloses a deep processing method of diversified development of an edible fungus and the derivatives thereof; the invention adopts the following steps: raw material treatment, selecting, washing, tray filling, cooling, temperature raising, a first time separation, a second time separation and derivatives processing, etc. By cooling the edible fungus body, the free water and the bound water are separated from the edible fungus body, and therefore the juice of the fresh edible fungus are obtained; using a sharp tool to cut off the connection part of the umbrella-shaped fruit body and the stalk filament body of the edible fungus to get the fruit body (umbrella part) of the edible fungus and the filament body (stalk part) of the edible fungus. Then, the edible fungus juice, the fruit body and the stalk body obtained from the processing method respectively are deep processed to produce more derivatives. The method has the advantages of simple in operation, high in juice yield, and suitable for diversified development and industrialized production.

The invention belongs to the technical field of artificial cultivation of edible fungi, particularly relates to an artificially cultivated phellinus igniarius sporocarp extract for treating gout, andfurther discloses a preparation method of the artificially cultivated phellinus igniarius sporocarp extract. The artificially cultivated phellinus igniarius sporocarp extract for treating gout is obtained by adopting an artificial bag material for efficiently cultivating a selected phellinus igniarius sporocarp raw material, cultivating phellinus igniarius sporocarp based on a traditional artificial bag material type cultivation mode, and taking the phellinus igniarius sporocarp as the raw material for ethanol extraction, and the obtained extract has a good uric acid reducing effect, and can be used for treatment gout.

The invention belongs to the field of fungus cultivation and provides a lucid ganoderma fungus purification and rejuvenation cultural method and a culture medium thereof against the problem that the activity of lucid ganoderma fungi is lowered due to an existing traditional lucid ganoderma fungus culture medium. The cultural method comprises the following steps: 1, preparing the lucid ganoderma fungus rejuvenation culture medium, wherein the lucid ganoderma fungus rejuvenation culture medium is prepared from 150-400 g of potatoes, 10-30 g of glucose, 10-25 g of agar powder, 1-5 g of monopotassium phosphate, 0.5-3 g of magnesium sulfate and 30-70 g of lucid ganoderma sporocarp; 2, culturing lucid ganoderma fungi into hypha, and then inoculating the hypha to the lucid ganoderma fungus rejuvenation culture medium obtained in step 1; 3, conducting culturing according to normal conditions, and obtaining rejuvenation fungi. The hypha obtained through the cultural method is quick in growth, pure white, orderly and dense, and the obtained sporocarp is good in agronomic trait.

The invention relates to an application of a chitosan-oligosaccharide containing composition to the production of edible fungi and particularly provides a culture solution for producing an edible fungi. The culture solution comprises a growth factor, a carbon source, a nitrogen source, an inorganic salt and water, wherein the growth factor comprises chitosan oligosaccharide and humic acid which are at the weight ratio of 1:(20-100); the growth factor accounts for 5-15% of the total weight of the culture solution, the carbon source accounts for 10-30% of the total weight of the culture solution, the nitrogen source accounts for 10-30% of the total weight of the culture solution, the inorganic salt accounts for 1-10% of the total weight of the culture solution, and the balance of water. The invention provides the application of the composition containing the chitosan oligosaccharide and the humic acid to the production of a variety of edible fungi. The method for culturing the edible fungi comprises the steps of culturing the culture solution containing the chitosan oligosaccharide and the humic acid and atomizing by using an edible fungus bag. The growth of the mycelium can be promoted, the yield of the fruiting body can be increased, and the quality of the fruiting body can be improved.

The invention discloses a method for producing cordyceps militaris sporocarp and a fish feed additive at the same time. A culture medium formula for producing the cordyceps militaris sporocarp comprises liquid A and solid B; the liquid A is prepared from the following components of 1000ml of water, 10.0 to 15.0g of saccharose, 0.3 to 0.5g of KH2PO4, 0.3 to 0.5g of MgSO4, four tablets of complex vitamin B and 1 tablet of vitamin C; the solid B is prepared from the following materials of 50% of wheat berry, 15% of rapeseed meal, 24% of peanut meal, 1% of dry silkworm chrysalis meal and 10% of rice bran; a ratio of the liquid A to the solid B powder is (2 to 3) to 1. The method comprises the steps of sealing, sterilizing and cooling the culture medium; then inoculating cordyceps militaris strain, inoculating according to a volume ratio of the strain to the culture medium as 1 to 50 and performing dark culture at first and then performing light culture in cultivation, wherein a strain density is 10 to 20 fungus balls / mL; obtaining the sporocarp and fungi residues. A crude polysaccharide content of the fungi residues reaches 27.43%, the fungi residues are naturally dried or stoved and then smashed to 80-mesh granularity, and digestion and absorption of fish bodies are facilitated; furthermore, the fungi residues have fragrant smell and can serve as the fish feed addition, and an adding amount is 10 to 15%.

The invention relates to a culture method for inhibiting the browning of bolete mycelium and belongs to the technical field of organism (macro fungi culture). The artificial culture of bolete is difficult to realize at present, and bolete fruiting bodies completely rely on wild picking. In recent years, because of the reasons of climate change, ecological damage and the like, the natural yield isseriously reduced, and the price is high. The growth of bolete hyphae on an artificial culture medium is slow, the culture medium and the hyphae easily brown after the hyphae grow for a period of time, and the subsequent growth is influenced. According to the method provided by the invention, a strain of unopened boletus edulis fruiting body produced in Yunnan is adopted as materials, paths such as mycelium inducing, mycelium activation and the like are adopted, and a culture medium capable of inhabiting or reducing the browning is provided. The culture medium has the characteristics that ingredients are clear and simple, the cost is low, the growth of the mycelium is fast, the bacterial colony is white and clean, and the like. Important foundation is laid for the scaled culture, artificial or semi-artificial domestication and the like of the bolete mycelium.

The invention relates to a hericium erinaceus mother strain culture medium. The hericium erinaceus mother strain culture medium is characterized in that melissyl alcohol with certain concentration is added to a PDA (Potato Dextrose Agar) enriched medium. The invention also relates to a making method of the hericium erinaceus mother strain culture medium. Compared with an existing PDA culture medium, the hericium erinaceus mother strain culture medium disclosed by the invention has the advantages that the growth vitality of hericium erinaceus mycelium is enhanced because the melissyl alcohol stimulates the energy metabolism of mycelium, so that the fungi culture time of a mother strain is shortened, the fungi culture time of an original strain is also shortened by making a hericium erinaceus original strain by using the mother strain, therefore, the differentiation phenomenon of a sporocarp during a strain germination period is avoided, the nutrient loss is reduced and the strain quality is improved.

The present invention provides compositions and methods for regulating expression of nucleotide sequences in fungi. Compositions are novel nucleotide sequences for a tissue preferred promoter isolated from the Agaricus bisporus lectin gene. The sequences drive expression preferentially to fruit body tissue. A method for expressing a nucleotide sequence in fungi using the regulatory sequences disclosed herein is provided. The method comprises transforming a fungal cell to comprise a nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed fungus from the transformed cell.

The invention relates to an establishing method for indoor symbiotic relationship of amanita flavipes and cyclobalanopsis glaucoides which is a symbiotic plant, and belongs to the field of biotechnology (plant tissues and Fungus cultivation). An overwhelming majority of amanita belongs to ectomycorrhizal fungi, can not be yet subjected to artificial cultivation at present, and can be hardly developped and utilized; and growth and development of sporocarps of the amanita has a close relationship with cyclobalanopsis glaucoids which is a symbiotic plant. The establishing method is characterizedin that myceliums are induced through the sporocarps of amanita flavipes for reproduction; aseptic seedlings and potted seedlings are successfully obtained by using seeds of cyclobalanopsis glaucoides, collected in the field; reproduced myceliums are inoculated to the roots of the aseptic seedlings and the potted seedlings respectively; the result shows that inoculated seedlings are more exuberant than the non-inoculated seedlings and have more leaves, the leaves of inoculated seedlings are dark green, disease resistance of inoculated seedlings are reinforced; and therefore, the indoor symbiotic relationship of amanita flavipes and cyclobalanopsis glaucoides is innovatively established, and a technological reserve is provided for future researches on symbiotic mechanism and mechanism generated in the sporocarps of amanita.

The invention discloses a hypsizigus marmoreus spawn quality detection method, which can solve the problems which are difficult to overcome by the present edible fungi spawn quality evaluation method such as systematicness, simplicity, convenience, high efficiency and the like. Due to the adoption of the detection method, the correlation of the evaluation result to yield traits such as yield of a single bottle, valid sporocarp quantity, weight of single sporocarp and the like can reach a remarkable level, the correlation of the evaluation result to mycelium growth speed in an identical test tube cultivation culture medium can reach an ordinary remarkable level, so spawn quality evaluation indexes can be separately evaluated through the method. At the same time, the evaluation indexes are adopted as a constitutional part to carry out the integrated quantitative evaluation on the hypsizigus marmoreus spawn quality, so the systematicness, the simplicity, the convenience and the high efficiency in evaluating the hypsizigus marmoreus spawn can be realized. In addition, the evaluation method provides a powerful reference for the integrated quantitative evaluation of other edible fungi spawn quality.

The invention discloses a method for rapidly propagating wild toadstool small leopard amanita mycelium indoor, and belongs to the technical field of cultivation of macrofungi. The method is characterized in that malt extract, glutamic acid and active substances for growth are simply added in young sporocarp mediotrastum induced mycelium before depucelation so that the mycelium can be subjected to rapid mass propagation. According to the invention, a simple culture medium is adopted, the cultivation period is short, the cost is low, and solid culture of the small leopard amanita mycelium can be realized on a large scale. The genus amanita is a large economic fungi which is special and precious and has great value and a wide application range in potentially developing new specific medicines such as anti-tumor medicines, antibacterial and antiviral medicines, sedatives, and narcotics; however, the genus amanita is difficult to develop and apply because the problems that resource of the genus amanita is rare, the genus amanita is difficult to artificially domesticate and the chemical synthetic of toxin is inactive. Aiming to the problems that the amanita mycelium is difficult to induce and grows slowly, the invention brings an innovative breakthrough and provides conditions to large-scale culture and artificial domestication of mycelium and the like.

The invention relates to a black fungus oligosaccharide for preventing liver cell oxidative damage as well as a preparation method and application thereof, and belongs to the field of preparation of effective components of edible fungi. The black fungus oligosaccharide is prepared from dried black fungus fruit bodies. The preparation method comprises following steps: rapid homogenization in combination with enzymatic extraction; extrusion filtration and protein removal; and ion exchange chromatography. The preparation method has the advantages that the extraction time is shortened, the reagentconsumption is reduced, the energy is saved, the solubility is improved, and the adopted extrusion filtration method is suitable for separating a viscous solution with a high colloid content; meanwhile, the black fungus oligosaccharide has the function of preventing liver cell oxidative damage and can be used for preparing liver-protecting and liver-protecting auxiliary medicines and health-carefoods, basic data for development of black fungus deep-processed products is provided, and the black fungus oligosaccharide has important economic and market values.

The invention belongs to the technical field of cultivation of valuable and rare medicinal fungi, and discloses a new strain of Ganoderma helvetianum and an artificial cultivation method thereof. The new strain of the Ganoderma hoehnelianum (HMGIM-D151177) is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on May 12, 2021, and the preservation number of the new strain of the Ganoderma hoehnelianum is GDMCC No: 61660. The artificial cultivation method of the new strain of the Ganoderma helvetianum comprises the steps of production mother strain manufacturing, production strain manufacturing, artificial domestication cultivation and cultivation management. According to the new strain of the Ganoderma helvetii and the artificial cultivation method thereof, the Ganoderma helvetianum is a new variety which is not researched yet, through separation and artificial domestication experiments of wild strains, the characteristics of artificial cultivation, good sporocarp character, high polysaccharide content and the like can be achieved, and the Ganoderma helvetianum is a strain with development prospects.

The invention provides an artificial cultivation method of Maowo fungi, which comprises the following steps: (1) selecting a wild Maowo fungi strain, and separating to obtain an Maowo fungi sporocarpas an Maowo fungi stock; (2) preparing a Imperata cylindrica root-PDA composite culture medium, placing the Imperata cylindrica root-PDA composite culture medium in a sterile culture container, inoculating of stock seeds, and culturing mother seeds; (3) uniformly mixing crushed fresh Imperata cylindrica, cottonseed hull, wheat bran and lime, bagging, sterilizing, cooling, inoculating of the Maowofungi mother strain, and culturing to obtain a Maowo fungi cultivar; (4) uniformly mixing fresh Imperata cylindrica, cottonseed hull, wheat bran and lime, adding water, piling and fermenting to prepare a culture medium; paving the culture medium on a greenhouse ground culture bed, sowing Maowo fungi cultivar, covering a film, and controlling greenhouse conditions to enable Maowo fungi to grow; (5)after Maowo fungi hyphae grow all over, removing the film, covering with strip-shaped fine soil belts, and spraying water on the soil covering layer until the soil covering layer is wet and thoroughly watered; and controlling greenhouse conditions, and harvesting fruiting bodies when the fruiting bodies of the Maowo fungi are flat so as to obtain Maowo fungi products.

The invention provides a method for cultivating inonotus hispidus by tremella mushroom bran, and belongs to the technical field of edible fungi cultivation. According to the method, a solid medium forcultivation comprises, in weight percent, 80%-100% of cotton seed hulls, 0%-20% of corncobs and 0%-20% of wheat bran, and the sum of the weight percents of all the components is 100%. A nutrient solution fluid medium comprises, in weight percent, 0.045%-0.055% of tremella waste mushroom bran powder, 1.8%-2.2% of glucose, 0.15%-0.25% of yeast extract powder and the balance water, the sum of the weight percents of all the components is 100%, and pH (potential of hydrogen) is natural. The solid medium and the fluid medium are mixed according to the weight ratio of 1:1.4 to 1:1.6. Inonotus hispidus sporocarps cultivated by the media have the advantages of short growth period, high quality and the like, and the media are simple to manufacture, low in cost and convenient to operate and easily realize factorization.

The invention provides an acclimatization cultivation method of trichaptum biforme, and belongs to the technical field of artificial acclimatization of fungi. The method comprises the following steps:firstly, performing isolated culture on wild trichaptum biforme sporocarp, so as to obtain mother strain mycelia; secondly, performing mother strain expanding propagation culture on the mother strainmycelia, so as to obtain propagated mother strain; thirdly, performing original strain culture on a propagated mother strain fungus block, so as to obtain original strain; fourthly, performing cultivated species culture on the original strain, so as to obtain cultivated species; fifthly, performing acclimatization cultivation culture on the cultivated species. The method has the advantages that the mycelia growth speed is high, and the mycelia are good in growing and dense; primordium is produced within 14 to 17d, small sporocarp is formed within 7 to 10d, and normal sporocarp is formed within 10 to 15d, and the yield of sporocarp is high. The trichaptum biforme sporocarp obtained through artificial cultivation is nontoxic, and sporocarp polysaccharide has obvious in vitro anti-hepatoma activity.

The invention discloses a high-quality cultivation method of grifola frondosa. The cultivation method comprises the following steps of: preparing a culture material, preparing a fungus bag, sterilizing at high temperature, namely sterilizing by adopting high-pressure steam, vacuumizing for three times, preserving heat at 95-105 DEG C for 55-65 minutes, preserving heat at 120-125 DEG C for 75-85 minutes, and braising for 18-25 minutes; cooling and inoculating; culturing hyphae; scratching fungi, and accelerating germination; managing in a fruiting period; and harvesting, namely harvesting whenthe diameter of a fruiting body leaf reaches 2.5-3.5 cm. The growth period is short, and the utilization rate of a plant is improved; the biotransformation rate is high, and fruiting is stable; the raw material types are few, and the cost is low. Most of the raw materials used in the cultivation method are crop leftovers and are convenient to purchase.

The invention relates to fungi and methods of isolated culture, preservation and rejuvenation, and discloses Xylaria gracillima (CGMCC No. 1625) and methods of isolated culture, preservation and rejuvenation; the isolated culture method is as follows: the main nest of odontotermes formosanus which is formed for more than three years are taken out of the natural soil layer to be put into an aseptic container, after being fully enclosed, mature fruiting bodies of the Xylaria gracillima are separated out and then are inoculated on the improved culture medium to isolate and culture the Xylaria gracillima; the preservation and rejuvenation method is as follows: after the Xylaria gracillima preserved in a medical freezer recovers, the Xylaria gracillima are inoculated to a fresh inclined culture medium in an asepsis room, the inclined culture medium is put in a freezer of 4 DEG C to be preserved after the hypha of the Xylaria gracillima overgrows on the inclined planes. The invention leads to species increasing of Xylaria, and solves the problems of difficult isolation and extraction and difficult mass industrial production of the Xylaria gracillima.

The invention provides a method for detecting neutral triterpenes in ganoderma fungi. The method comprises the following steps: (1) pretreating ganoderma lucidum sporocarp; (2) performing an ultra-high performance liquid chromatography detection; (3) obtaining mass spectrum information of the compound; (4) establishing an ultra-high performance liquid chromatography-triple quadrupole mass spectrometry combined analysis method; (5) performing data analysis. According to the method for simultaneously detecting the 12 neutral triterpenoids in the ganoderma fungus, the 12 neutral triterpenoids in the ganoderma fungus are qualitatively and quantitatively analyzed simultaneously, and accurate and reliable detection analysis is provided for related research of the ganoderma fungus.

The invention discloses absolute perenniporia robiniophila (murrill) ravarden oil containing ambrettolide and a preparation method and application thereof. The absolute oil is derived from the perenniporia robiniophila (murrill) ravarden (Perenniporia robiniophila (Murrill) Ravarden) as medical fungi. Perenniporia robiniophila (murrill) ravarden sporophores are subjected to extraction by an organic solvent, after extracted liquid is cooled and dewaxed, the solvent is recovered, and the absolute perenniporia robiniophila (murrill) ravarden oil is obtained. Through gas chromatography-mass spectrum combination analysis, feature components of the absolute soil are the ambrettolide, (Z)-11-zoomaric acid, 1-oxygen heterocycle tetradecane-2-ketone and 2-hydroxy cyclopentadecanone, and the absolute oil has strong and elegant musk fragrance, and can be applied to daily and edible essence to be a fixative and a synergist and used as a raw material for preparing a pure ambrettolide product.

The invention discloses a preparation method of an antrodia extract, and belongs to the technical field of application of edible and medicinal fungi. The method comprises extracting vessel-cultured antrodia sporocarp powder with an ethanol solution and conducting vacuum concentration on a filtrate to obtain a crude extract; adsorbing the crude extract with macroporous resin, placing the mixture ina rectangular container, washing the mixture out with a mixed solution, conducting vacuum concentration on an eluent, drying to obtain a first sub extract of the antrodia contract, then washing out with the ethanol solution, conducting vacuum concentration on the eluent, drying to obtain a second sub extract of the antrodia contract, washing out with an ethyl acetate solution, conducting vacuum concentration on the eluent and drying to obtain a third sub extract of the antrodia contract. The invention also provides a preparation method for an antrodia composition and a pharmaceutical composition. The sub extracts of the antrodia extract or the antrodia composition can be used for treating cancer and reducing side effects of chemotherapy.

The invention discloses (Bosea sp.) Y4 and application thereof for accelerating Hypsizygus marmoreus(Peck)H .E .Bigelow to grow, and relates to the biotechnology field of edible fungi. The (Bosea sp.)Y4 is collected in the China Center for Type Culture Collection with a collection number CCTCC NO:M 2019634, wherein a collection date is 16th, August, 2019, and a collection address is Wuhan University, China. The (Bosea sp.) Y4 is used for accelerating the Hypsizygus marmoreus(Peck)H .E .Bigelow to grow. Through bacteria solution culture, cultivation application and fruiting management, the Hypsizygus marmoreus(Peck)H .E .Bigelow in which the (Bosea sp.) Y4 is added is characterized in that a hypha growth rate is increased, the diameter of a sporocarp pileus is increased, a stipe is long, time for a bottle to be full is shortened and a bottle yield is increased. The growth rate of the Hypsizygus marmoreus(Peck)H .E .Bigelow can be effectively improved, the production period of the Hypsizygus marmoreus(Peck)H .E .Bigelow is shortened, the yield of the Hypsizygus marmoreus(Peck)H .E .Bigelow can be improved, a product is guaranteed to be environmentally-friendly, healthy and free frompollution, and the production efficiency and the social benefit of the Hypsizygus marmoreus(Peck)H .E .Bigelow can be improved.