91 results about "Sporocarp (fungi)" patented technology

The invention belongs to the field of microbial fungi, and relates to a selenium-rich cordyceps militaris strain CM001; the wild cordyceps militaris strain which is newly separated in the nature is selected as an original strain; the original strain is subjected to ultraviolet mutation, and the strain can be obtained by separating and screening; the selenium-rich cordyceps militaris strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 18, 2014 with the preservation registration number of CGMCC No.9460, is named as CM001 and is named as cordyceps militaris in a classified way. Under the artificial culture conditions, the cordyceps militaris strain has the tolerance for high-concentration selenium, and can carry out biotransformation in a body; the hyphae yeast, the growth matrix or the sporocarp of the selenium-rich cordyceps militaris strain can be used as raw materials of medicines, foods and drinks, or can be prepared into powder, tablets, freeze-drying powder, capsules or electuary.

The invention provides a living Chinese caterpillar fungi artificially cultivated by using larvae as hosts, which comprise insect larvae and one or two about two centimeter living Chinese caterpillar fungus fruiting bodies grown on the head of each the larva. The cultivating process comprises the steps of: invasion of the Chinese caterpillar fungi into the living insect larvae, in vivo growth of the hyphae in the living insect larvae, inactivation of the larvae, growth of the Chinese caterpillar fungus fruiting bodies on the heads and the like; and in the whole process, not only the insect larvae and the hyphae are grown in the polypide, but also the Chinese caterpillar fungus fruiting bodies are grown on the heads, the obtained Chinese caterpillar fungi have the same appearance and almost the same nutrient content as the wild Chinese caterpillar fungi, so that the Chinese caterpillar fungi obtained by the method are the real living Chinese caterpillar fungi obtained by artificial cultivation.

The invention discloses a deep processing method of diversified development of an edible fungus and the derivatives thereof; the invention adopts the following steps: raw material treatment, selecting, washing, tray filling, cooling, temperature raising, a first time separation, a second time separation and derivatives processing, etc. By cooling the edible fungus body, the free water and the bound water are separated from the edible fungus body, and therefore the juice of the fresh edible fungus are obtained; using a sharp tool to cut off the connection part of the umbrella-shaped fruit body and the stalk filament body of the edible fungus to get the fruit body (umbrella part) of the edible fungus and the filament body (stalk part) of the edible fungus. Then, the edible fungus juice, the fruit body and the stalk body obtained from the processing method respectively are deep processed to produce more derivatives. The method has the advantages of simple in operation, high in juice yield, and suitable for diversified development and industrialized production.

The invention belongs to the field of fungus cultivation and provides a lucid ganoderma fungus purification and rejuvenation cultural method and a culture medium thereof against the problem that the activity of lucid ganoderma fungi is lowered due to an existing traditional lucid ganoderma fungus culture medium. The cultural method comprises the following steps: 1, preparing the lucid ganoderma fungus rejuvenation culture medium, wherein the lucid ganoderma fungus rejuvenation culture medium is prepared from 150-400 g of potatoes, 10-30 g of glucose, 10-25 g of agar powder, 1-5 g of monopotassium phosphate, 0.5-3 g of magnesium sulfate and 30-70 g of lucid ganoderma sporocarp; 2, culturing lucid ganoderma fungi into hypha, and then inoculating the hypha to the lucid ganoderma fungus rejuvenation culture medium obtained in step 1; 3, conducting culturing according to normal conditions, and obtaining rejuvenation fungi. The hypha obtained through the cultural method is quick in growth, pure white, orderly and dense, and the obtained sporocarp is good in agronomic trait.

The invention discloses a method for producing cordyceps militaris sporocarp and a fish feed additive at the same time. A culture medium formula for producing the cordyceps militaris sporocarp comprises liquid A and solid B; the liquid A is prepared from the following components of 1000ml of water, 10.0 to 15.0g of saccharose, 0.3 to 0.5g of KH2PO4, 0.3 to 0.5g of MgSO4, four tablets of complex vitamin B and 1 tablet of vitamin C; the solid B is prepared from the following materials of 50% of wheat berry, 15% of rapeseed meal, 24% of peanut meal, 1% of dry silkworm chrysalis meal and 10% of rice bran; a ratio of the liquid A to the solid B powder is (2 to 3) to 1. The method comprises the steps of sealing, sterilizing and cooling the culture medium; then inoculating cordyceps militaris strain, inoculating according to a volume ratio of the strain to the culture medium as 1 to 50 and performing dark culture at first and then performing light culture in cultivation, wherein a strain density is 10 to 20 fungus balls/mL; obtaining the sporocarp and fungi residues. A crude polysaccharide content of the fungi residues reaches 27.43%, the fungi residues are naturally dried or stoved and then smashed to 80-mesh granularity, and digestion and absorption of fish bodies are facilitated; furthermore, the fungi residues have fragrant smell and can serve as the fish feed addition, and an adding amount is 10 to 15%.

The invention relates to a culture method for inhibiting the browning of bolete mycelium and belongs to the technical field of organism (macro fungi culture). The artificial culture of bolete is difficult to realize at present, and bolete fruiting bodies completely rely on wild picking. In recent years, because of the reasons of climate change, ecological damage and the like, the natural yield isseriously reduced, and the price is high. The growth of bolete hyphae on an artificial culture medium is slow, the culture medium and the hyphae easily brown after the hyphae grow for a period of time, and the subsequent growth is influenced. According to the method provided by the invention, a strain of unopened boletus edulis fruiting body produced in Yunnan is adopted as materials, paths such as mycelium inducing, mycelium activation and the like are adopted, and a culture medium capable of inhabiting or reducing the browning is provided. The culture medium has the characteristics that ingredients are clear and simple, the cost is low, the growth of the mycelium is fast, the bacterial colony is white and clean, and the like. Important foundation is laid for the scaled culture, artificial or semi-artificial domestication and the like of the bolete mycelium.

The invention relates to a hericium erinaceus mother strain culture medium. The hericium erinaceus mother strain culture medium is characterized in that melissyl alcohol with certain concentration is added to a PDA (Potato Dextrose Agar) enriched medium. The invention also relates to a making method of the hericium erinaceus mother strain culture medium. Compared with an existing PDA culture medium, the hericium erinaceus mother strain culture medium disclosed by the invention has the advantages that the growth vitality of hericium erinaceus mycelium is enhanced because the melissyl alcohol stimulates the energy metabolism of mycelium, so that the fungi culture time of a mother strain is shortened, the fungi culture time of an original strain is also shortened by making a hericium erinaceus original strain by using the mother strain, therefore, the differentiation phenomenon of a sporocarp during a strain germination period is avoided, the nutrient loss is reduced and the strain quality is improved.

The present invention provides compositions and methods for regulating expression of nucleotide sequences in fungi. Compositions are novel nucleotide sequences for a tissue preferred promoter isolated from the Agaricus bisporus lectin gene. The sequences drive expression preferentially to fruit body tissue. A method for expressing a nucleotide sequence in fungi using the regulatory sequences disclosed herein is provided. The method comprises transforming a fungal cell to comprise a nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed fungus from the transformed cell.

The invention relates to an establishing method for indoor symbiotic relationship of amanita flavipes and cyclobalanopsis glaucoides which is a symbiotic plant, and belongs to the field of biotechnology (plant tissues and Fungus cultivation). An overwhelming majority of amanita belongs to ectomycorrhizal fungi, can not be yet subjected to artificial cultivation at present, and can be hardly developped and utilized; and growth and development of sporocarps of the amanita has a close relationship with cyclobalanopsis glaucoids which is a symbiotic plant. The establishing method is characterizedin that myceliums are induced through the sporocarps of amanita flavipes for reproduction; aseptic seedlings and potted seedlings are successfully obtained by using seeds of cyclobalanopsis glaucoides, collected in the field; reproduced myceliums are inoculated to the roots of the aseptic seedlings and the potted seedlings respectively; the result shows that inoculated seedlings are more exuberant than the non-inoculated seedlings and have more leaves, the leaves of inoculated seedlings are dark green, disease resistance of inoculated seedlings are reinforced; and therefore, the indoor symbiotic relationship of amanita flavipes and cyclobalanopsis glaucoides is innovatively established, and a technological reserve is provided for future researches on symbiotic mechanism and mechanism generated in the sporocarps of amanita.

The invention discloses a method for rapidly propagating wild toadstool small leopard amanita mycelium indoor, and belongs to the technical field of cultivation of macrofungi. The method is characterized in that malt extract, glutamic acid and active substances for growth are simply added in young sporocarp mediotrastum induced mycelium before depucelation so that the mycelium can be subjected to rapid mass propagation. According to the invention, a simple culture medium is adopted, the cultivation period is short, the cost is low, and solid culture of the small leopard amanita mycelium can be realized on a large scale. The genus amanita is a large economic fungi which is special and precious and has great value and a wide application range in potentially developing new specific medicines such as anti-tumor medicines, antibacterial and antiviral medicines, sedatives, and narcotics; however, the genus amanita is difficult to develop and apply because the problems that resource of the genus amanita is rare, the genus amanita is difficult to artificially domesticate and the chemical synthetic of toxin is inactive. Aiming to the problems that the amanita mycelium is difficult to induce and grows slowly, the invention brings an innovative breakthrough and provides conditions to large-scale culture and artificial domestication of mycelium and the like.

The invention belongs to the technical field of cultivation of valuable and rare medicinal fungi, and discloses a new strain of Ganoderma helvetianum and an artificial cultivation method thereof. The new strain of the Ganoderma hoehnelianum (HMGIM-D151177) is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on May 12, 2021, and the preservation number of the new strain of the Ganoderma hoehnelianum is GDMCC No: 61660. The artificial cultivation method of the new strain of the Ganoderma helvetianum comprises the steps of production mother strain manufacturing, production strain manufacturing, artificial domestication cultivation and cultivation management. According to the new strain of the Ganoderma helvetii and the artificial cultivation method thereof, the Ganoderma helvetianum is a new variety which is not researched yet, through separation and artificial domestication experiments of wild strains, the characteristics of artificial cultivation, good sporocarp character, high polysaccharide content and the like can be achieved, and the Ganoderma helvetianum is a strain with development prospects.

The invention provides an artificial cultivation method of Maowo fungi, which comprises the following steps: (1) selecting a wild Maowo fungi strain, and separating to obtain an Maowo fungi sporocarpas an Maowo fungi stock; (2) preparing a Imperata cylindrica root-PDA composite culture medium, placing the Imperata cylindrica root-PDA composite culture medium in a sterile culture container, inoculating of stock seeds, and culturing mother seeds; (3) uniformly mixing crushed fresh Imperata cylindrica, cottonseed hull, wheat bran and lime, bagging, sterilizing, cooling, inoculating of the Maowofungi mother strain, and culturing to obtain a Maowo fungi cultivar; (4) uniformly mixing fresh Imperata cylindrica, cottonseed hull, wheat bran and lime, adding water, piling and fermenting to prepare a culture medium; paving the culture medium on a greenhouse ground culture bed, sowing Maowo fungi cultivar, covering a film, and controlling greenhouse conditions to enable Maowo fungi to grow; (5)after Maowo fungi hyphae grow all over, removing the film, covering with strip-shaped fine soil belts, and spraying water on the soil covering layer until the soil covering layer is wet and thoroughly watered; and controlling greenhouse conditions, and harvesting fruiting bodies when the fruiting bodies of the Maowo fungi are flat so as to obtain Maowo fungi products.

The invention provides an acclimatization cultivation method of trichaptum biforme, and belongs to the technical field of artificial acclimatization of fungi. The method comprises the following steps:firstly, performing isolated culture on wild trichaptum biforme sporocarp, so as to obtain mother strain mycelia; secondly, performing mother strain expanding propagation culture on the mother strainmycelia, so as to obtain propagated mother strain; thirdly, performing original strain culture on a propagated mother strain fungus block, so as to obtain original strain; fourthly, performing cultivated species culture on the original strain, so as to obtain cultivated species; fifthly, performing acclimatization cultivation culture on the cultivated species. The method has the advantages that the mycelia growth speed is high, and the mycelia are good in growing and dense; primordium is produced within 14 to 17d, small sporocarp is formed within 7 to 10d, and normal sporocarp is formed within 10 to 15d, and the yield of sporocarp is high. The trichaptum biforme sporocarp obtained through artificial cultivation is nontoxic, and sporocarp polysaccharide has obvious in vitro anti-hepatoma activity.

The invention discloses a high-quality cultivation method of grifola frondosa. The cultivation method comprises the following steps of: preparing a culture material, preparing a fungus bag, sterilizing at high temperature, namely sterilizing by adopting high-pressure steam, vacuumizing for three times, preserving heat at 95-105 DEG C for 55-65 minutes, preserving heat at 120-125 DEG C for 75-85 minutes, and braising for 18-25 minutes; cooling and inoculating; culturing hyphae; scratching fungi, and accelerating germination; managing in a fruiting period; and harvesting, namely harvesting whenthe diameter of a fruiting body leaf reaches 2.5-3.5 cm. The growth period is short, and the utilization rate of a plant is improved; the biotransformation rate is high, and fruiting is stable; the raw material types are few, and the cost is low. Most of the raw materials used in the cultivation method are crop leftovers and are convenient to purchase.

The invention provides a cultivation medium and an industrial cultivation method for tremella fuciformis. The cultivation medium is prepared from following components in percentage by mass: 67%-72% of fresh Lvzhou No.1 fungus grass, 25%-30% of wheat bran, 0.8%-1.2% of gypsum, 0.8%-1.2% of lime, 0.1%-0.4% of salt and 0.1%-0.4% of calcium superphosphate, wherein the water content of the fresh Lvzhou No.1 fungus grass is 70-75%. The cultivation method comprises the steps of cultivation medium preparation, sterilization, inoculation, mycelium culture, sporocarp growth and harvesting. According to the technical scheme, the fresh Lvzhou No.1 fungus grass is used for cultivation, the traditional theory that edible and medicinal fungi cannot be directly cultivated through fresh raw materials is broken through, the Lvzhou No.1 fungus grass is adopted as the main raw material, the method is green and environmentally friendly, the ecology is protected, the yield of obtained tremella fuciformis sporocarp is high, and the polysaccharide content and the total flavone content can reach 80.88 mg/g and 8.52 mg/g.

The invention provides a method for detecting neutral triterpenes in ganoderma fungi. The method comprises the following steps: (1) pretreating ganoderma lucidum sporocarp; (2) performing an ultra-high performance liquid chromatography detection; (3) obtaining mass spectrum information of the compound; (4) establishing an ultra-high performance liquid chromatography-triple quadrupole mass spectrometry combined analysis method; (5) performing data analysis. According to the method for simultaneously detecting the 12 neutral triterpenoids in the ganoderma fungus, the 12 neutral triterpenoids in the ganoderma fungus are qualitatively and quantitatively analyzed simultaneously, and accurate and reliable detection analysis is provided for related research of the ganoderma fungus.

The invention discloses absolute perenniporia robiniophila (murrill) ravarden oil containing ambrettolide and a preparation method and application thereof. The absolute oil is derived from the perenniporia robiniophila (murrill) ravarden (Perenniporia robiniophila (Murrill) Ravarden) as medical fungi. Perenniporia robiniophila (murrill) ravarden sporophores are subjected to extraction by an organic solvent, after extracted liquid is cooled and dewaxed, the solvent is recovered, and the absolute perenniporia robiniophila (murrill) ravarden oil is obtained. Through gas chromatography-mass spectrum combination analysis, feature components of the absolute soil are the ambrettolide, (Z)-11-zoomaric acid, 1-oxygen heterocycle tetradecane-2-ketone and 2-hydroxy cyclopentadecanone, and the absolute oil has strong and elegant musk fragrance, and can be applied to daily and edible essence to be a fixative and a synergist and used as a raw material for preparing a pure ambrettolide product.

The invention discloses (Bosea sp.) Y4 and application thereof for accelerating Hypsizygus marmoreus(Peck)H .E .Bigelow to grow, and relates to the biotechnology field of edible fungi. The (Bosea sp.)Y4 is collected in the China Center for Type Culture Collection with a collection number CCTCC NO:M 2019634, wherein a collection date is 16th, August, 2019, and a collection address is Wuhan University, China. The (Bosea sp.) Y4 is used for accelerating the Hypsizygus marmoreus(Peck)H .E .Bigelow to grow. Through bacteria solution culture, cultivation application and fruiting management, the Hypsizygus marmoreus(Peck)H .E .Bigelow in which the (Bosea sp.) Y4 is added is characterized in that a hypha growth rate is increased, the diameter of a sporocarp pileus is increased, a stipe is long, time for a bottle to be full is shortened and a bottle yield is increased. The growth rate of the Hypsizygus marmoreus(Peck)H .E .Bigelow can be effectively improved, the production period of the Hypsizygus marmoreus(Peck)H .E .Bigelow is shortened, the yield of the Hypsizygus marmoreus(Peck)H .E .Bigelow can be improved, a product is guaranteed to be environmentally-friendly, healthy and free frompollution, and the production efficiency and the social benefit of the Hypsizygus marmoreus(Peck)H .E .Bigelow can be improved.