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Xylaria gracillima and methods of isolated culture, preservation and rejuvenation

A kind of carbon horn bacteria, slender technology, applied in the direction of botany equipment and methods, fungal products, fertilizer mixture, etc., can solve the problems such as difficult separation and extraction, few species of carbon horn bacteria

Active Publication Date: 2010-02-24
浙江三禾生物工程股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims at the problem that there are few types of Pyrethymus spp. in the prior art and it is difficult to separate and extract, and it provides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, the method for isolation and culture of C. slenderus comprises the following steps:

[0025] Step 1: Take out the main nest of the black-winged white mosquito that has been nesting for more than three years from the natural underground soil layer, use sterile instruments to take out the main nest of the black-winged white mosquito from the ground, and put it directly into a sterile container In the container, immediately fully enclosed, put it in an environment of 5°C, and make the black winged mosquito in the main nest of the black winged mosquito die naturally. After the black winged mosquito dies, the carbon dioxide gas in the container The volume ratio of the container is increased from 0.03% to 1%, and the container is fully closed again, and the container is stored in a dark environment at 20°C;

[0026] Step 2: After 5 days, when the dominant bacteria of C. slenderus gradually appear in the symbiosis of the main nest of the black winged white mosqui...

Embodiment 2

[0031] Embodiment 2, the method for isolation and culture of C. slenderus comprises the following steps:

[0032] Step 1: Take out the main nest of the black-winged white mosquito that has been nesting for more than three years from the natural underground soil layer, use sterile instruments to take out the main nest of the black-winged white mosquito from the ground, and put it directly into a sterile container In the container, immediately fully enclosed, put it in an environment of 5°C, and make the black winged mosquito in the main nest of the black winged mosquito die naturally. After the black winged mosquito dies, the carbon dioxide gas in the container The volume ratio of the container is increased from 0.03% to 2%, the container is fully closed again, and the container is stored in a dark environment at 20°C;

[0033] Step 2: After 10 days, when the dominant bacteria of C. slenderi gradually appear in the symbiosis of the main nest of the black-winged white mosquito, ...

Embodiment 3

[0038] Embodiment 3, the method for isolation and culture of C. slenderus comprises the following steps:

[0039] Step 1: Take out the main nest of the black-winged white mosquito that has been nesting for more than three years from the natural underground soil layer, use sterile instruments to take out the main nest of the black-winged white mosquito from the ground, and put it directly into a sterile container In the container, immediately fully enclosed, put it in an environment of 5°C, and make the black winged mosquito in the main nest of the black winged mosquito die naturally. After the black winged mosquito dies, the carbon dioxide gas in the container The volume ratio of the container is increased from 0.03% to 1.5%, and the container is fully closed again, and the container is stored in a dark environment at 20°C;

[0040] Step 2: After 8 days, when the dominant bacteria of Xylella slenderens gradually appear in the Xylella slender bacteria parasitizing on the main n...

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PUM

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Abstract

The invention relates to fungi and methods of isolated culture, preservation and rejuvenation, and discloses Xylaria gracillima (CGMCC No. 1625) and methods of isolated culture, preservation and rejuvenation; the isolated culture method is as follows: the main nest of odontotermes formosanus which is formed for more than three years are taken out of the natural soil layer to be put into an aseptic container, after being fully enclosed, mature fruiting bodies of the Xylaria gracillima are separated out and then are inoculated on the improved culture medium to isolate and culture the Xylaria gracillima; the preservation and rejuvenation method is as follows: after the Xylaria gracillima preserved in a medical freezer recovers, the Xylaria gracillima are inoculated to a fresh inclined culture medium in an asepsis room, the inclined culture medium is put in a freezer of 4 DEG C to be preserved after the hypha of the Xylaria gracillima overgrows on the inclined planes. The invention leads to species increasing of Xylaria, and solves the problems of difficult isolation and extraction and difficult mass industrial production of the Xylaria gracillima.

Description

technical field [0001] The present invention relates to bacterium and its separation culture, preservation and rejuvenation method, especially relates to Xylocarpus and its separation culture, preservation and rejuvenation method. Background technique [0002] Xylariaceae (Xylariaceae) is a family of Fungi, Ascomycotina, Sclerotinia, and Spheroides. Undergraduate fungi usually have sub-seats, which are sessile or stalked, and can often be upright. Born in the sub-seat, usually dark, with orifices, beaked, solitary or clustered, lateral filaments linear, branched, dissolving when mature, ascus single-walled, thin-walled, often thickened and crested Ring structure, turning blue when exposed to iodine, mostly columnar, rod-shaped, less spherical, rarely disintegrated, ascospore unit cell, brown to black, ovate, elliptic, spindle-shaped, etc., mostly with bud slits, sometimes There are colloidal appendages on the top, which shoot out or overflow in a sticky mass, and mostly rot...

Claims

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Application Information

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IPC IPC(8): A01H15/00A01G1/04C05G3/00
Inventor 朱志熊
Owner 浙江三禾生物工程股份有限公司
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