Xylaria gracillima and methods of isolated culture, preservation and rejuvenation
A kind of carbon horn bacteria, slender technology, applied in the direction of botany equipment and methods, fungal products, fertilizer mixture, etc., can solve the problems such as difficult separation and extraction, few species of carbon horn bacteria
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Embodiment 1
[0024] Embodiment 1, the method for isolation and culture of C. slenderus comprises the following steps:
[0025] Step 1: Take out the main nest of the black-winged white mosquito that has been nesting for more than three years from the natural underground soil layer, use sterile instruments to take out the main nest of the black-winged white mosquito from the ground, and put it directly into a sterile container In the container, immediately fully enclosed, put it in an environment of 5°C, and make the black winged mosquito in the main nest of the black winged mosquito die naturally. After the black winged mosquito dies, the carbon dioxide gas in the container The volume ratio of the container is increased from 0.03% to 1%, and the container is fully closed again, and the container is stored in a dark environment at 20°C;
[0026] Step 2: After 5 days, when the dominant bacteria of C. slenderus gradually appear in the symbiosis of the main nest of the black winged white mosqui...
Embodiment 2
[0031] Embodiment 2, the method for isolation and culture of C. slenderus comprises the following steps:
[0032] Step 1: Take out the main nest of the black-winged white mosquito that has been nesting for more than three years from the natural underground soil layer, use sterile instruments to take out the main nest of the black-winged white mosquito from the ground, and put it directly into a sterile container In the container, immediately fully enclosed, put it in an environment of 5°C, and make the black winged mosquito in the main nest of the black winged mosquito die naturally. After the black winged mosquito dies, the carbon dioxide gas in the container The volume ratio of the container is increased from 0.03% to 2%, the container is fully closed again, and the container is stored in a dark environment at 20°C;
[0033] Step 2: After 10 days, when the dominant bacteria of C. slenderi gradually appear in the symbiosis of the main nest of the black-winged white mosquito, ...
Embodiment 3
[0038] Embodiment 3, the method for isolation and culture of C. slenderus comprises the following steps:
[0039] Step 1: Take out the main nest of the black-winged white mosquito that has been nesting for more than three years from the natural underground soil layer, use sterile instruments to take out the main nest of the black-winged white mosquito from the ground, and put it directly into a sterile container In the container, immediately fully enclosed, put it in an environment of 5°C, and make the black winged mosquito in the main nest of the black winged mosquito die naturally. After the black winged mosquito dies, the carbon dioxide gas in the container The volume ratio of the container is increased from 0.03% to 1.5%, and the container is fully closed again, and the container is stored in a dark environment at 20°C;
[0040] Step 2: After 8 days, when the dominant bacteria of Xylella slenderens gradually appear in the Xylella slender bacteria parasitizing on the main n...
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