5results about How to "High analytical sensitivity" patented technology

This invention discloses a wide dynamic range substrate reagent, belonging to the field of biological detection reagent technology. The reagent consists of the following components: citric acid, 9.6 g / L; 3,3',5,5'-tetramethylbenzidine, 0.29 g / L; 3% hydrogen peroxide solution, 3 mL / L; 1 g / L polyvinyl alcohol; 0.5 g / L copovidone W-735; 0.5 g / L dimethyldiallylammonium chloride-acrylamide copolymer; 1 g / L PVP K30; and Tween 20, 0.005 g / L. mL / L; polyethylene glycol 10g-50g / L; pure water, balance. This invention, by adding specific polyethylene glycol and synergistically combining with other components, significantly reduces the reagent background signal and improves detection sensitivity; effectively broadens the detection dynamic range and increases the detection upper limit of high-concentration analytes; improves the linear correlation between signal and concentration; and enhances the thermally accelerated stability of the reagent. This reagent has comprehensive performance and is especially suitable for ELISA detection systems with high sensitivity and wide detection range.

The application discloses a mediator type integrated microbial electrode capable of detecting seawater biological toxicity, and belongs to the field of seawater biological toxicity detection. The electrode comprises an electrode base and a microbial membrane uniformly attached to the electrode base. The microbial membrane is a thin film obtained by fixing mixed and uniform electronic mediators and microorganisms on the electrode base through a gel solution and then drying. The gel solution is formed by mixing polydiallyldimethylammonium chloride solution and chitosan solution. The electrode can be used to quickly and accurately detect seawater biological toxicity, fills the blank of the mediator type integrated microbial electrode in the field of seawater toxicity evaluation, and opens up the application of the electrochemical method in marine water quality monitoring. The application further discloses a preparation method and application of the mediator type integrated microbial electrode.

This invention belongs to the field of power transmission capacity sensitivity analysis technology, specifically relating to a regional sensitivity analysis method for static maximum transmission capacity based on an improved 3D adaptive sparse grid interpolation method. The method includes: importing system parameters to obtain a deterministic MSTC calculation model; establishing a probabilistic model of uncertain sources in the power system and converting it into the required random input variables; correcting the boundaries of the random input variables to obtain a surrogate model for MSTC; generating MCS samples of random inputs to obtain the probability results of MSTC; calculating the CSM and CSV numerical results related to the random inputs of MSTC; selecting the top-ranking important random input variables; combining the corresponding CSM and CSV numerical results; analyzing the graphical expressions of the important random input variables; and determining adjustment strategies. This method efficiently identifies important random variables, thereby providing effective strategies for their adjustment and MSTC control, thus improving the stability and economic operation of the system.

The present invention relates to a method for performing a liquid phase immunoassay using a diagnostic test cartridge having a magnetic bead well, a reaction well, a washing well, and a measurement well. The liquid phase immunoassay method of the present invention includes a step of bringing a magnet close to a pipette tip to capture and transfer magnetic beads filled in the magnetic bead well to the reaction well, a step of providing a sample to the reaction well to perform an antigen-antibody reaction, a step of bringing the magnet close to the pipette tip to capture and transfer a reaction in the reaction well to the pipette tip and to the washing well to perform washing, and a step of bringing the magnet close to the pipette tip to transfer the reaction after washing in the washing well to the measurement well and to perform optical analysis. According to the present invention, the capture yield of magnetic beads can be improved.

This invention discloses an ionizing emissive for iron isotope analysis and its preparation and testing methods. The ionizing emissive comprises a silicon nanoparticle suspension, a phosphoric acid solution, and an aluminum nitrate solution, wherein the phosphoric acid solution and aluminum nitrate solution are used as emissive excipients. In the iron isotope analysis testing method, the silicon nanoparticle suspension is used as a high-sensitivity emissive to enhance the ionization efficiency of the iron sample. Simultaneously, the phosphoric acid solution and aluminum nitrate solution assist ionization, and a high-purity rhenium filament is used as the sample carrier to test trace amounts of iron isotopes. The filament ionization temperature is 1350–1450℃. This invention has the advantages of high sensitivity, simple operation, and low cost. Compared with the traditional thermal ionization mass spectrometry method which requires 4000–50000 ng of sample, this invention only requires 400–4000 ng to obtain good iron isotope testing accuracy (<0.02%, RSE), significantly reducing the amount of iron sample used while improving testing sensitivity.