23results about How to "High analytical sensitivity" patented technology

The utility model belongs to earth and planet chemical instrument equipment, and discloses a Nano-C isotope analysis device which comprises an element analysis system, a carbon dioxide freezing enrichment system and an isotope mass spectrometry system, the carbon dioxide freezing enrichment system comprises a six-way valve, the six-way valve is communicated with a first freezing assembly and a second freezing assembly, and the second freezing assembly is communicated with the isotope mass spectrometry system; the first freezing assembly comprises a first freezing container, and a first cold trap communicating with the six-way valve is arranged in the first freezing container in a liftable mode. The second freezing assembly comprises a second freezing container, and a second cold trap communicating with the six-way valve is arranged in the second freezing container in a liftable mode. The carbon isotope analyzer is simple and compact in mechanism, convenient to use, capable of analyzing carbon elements in different forms and accurately measuring isotope composition of carbon in different forms at the nanomole level, capable of enriching and purifying a sample to be detected for multiple times, capable of reducing analysis errors and capable of improving measurement sensitivity and precision.

The invention belongs to the technical field of analysis and detection, and relates to an analysis method and an analysis system of ethylene glycol by-product mixed alcohol, the analysis method comprises the following steps: S1, preparing a corresponding reference solution and a standard mixed alcohol solution according to a to-be-analyzed component in the ethylene glycol by-product mixed alcohol; s2, for the reference substance solution and the standard mixed alcohol solution, constructing a standard curve model corresponding to each component through gas chromatography; s3, collecting by-products in ethylene glycol production in real time, and filtering; according to the method, peak areas corresponding to components to be analyzed in the mixed alcohol sample are obtained and then substituted into respective standard curve models respectively, and the content of the components to be analyzed in the mixed alcohol sample is obtained. According to the method, the standard curve model is constructed, and the mixed alcohol is pretreated, so that the content of multiple components in the mixed alcohol is analyzed and detected, the analysis time is shortened, the analysis cost is reduced, and the analysis accuracy and sensitivity are improved.

The invention discloses a liquid chromatography method for determining the content of nine flavonoid compounds in a natural perfume raw material. The method is based on an ultra-high performance liquid chromatography technology, chromatographic conditions are optimized, two HSS T3 chromatographic columns are connected in series, and efficient separation of various flavonoid compounds is successfully achieved. The method is particularly suitable for analysis of complex samples, can be used for simultaneously determining the content of various flavonoid compounds in single detection, and has relatively high sensitivity and separation degree. In addition, the method is also combined with multi-wavelength detection, the flavonoid compounds in the sample are quantitatively analyzed through an external standard method, and efficient separation and accurate determination of the flavonoid compounds can be realized. Compared with a traditional LC / MS method, the method has the advantages that analysis efficiency and accuracy are improved, sample treatment steps and analysis cost are reduced, and the method has wide industrial application potential.

The invention belongs to the technical field of immunodiagnosis, and relates to a detection card for quantitatively detecting human total IgE antibodies, which comprises a card shell and a test strip, and the test strip is arranged in the card shell; the test strip comprises a sample pad, a combination pad, a reaction film and an absorption pad which are arranged on a bottom plate, wherein an animal anti-human IgE monoclonal antibody marked by lanthanide fluorescent microspheres is arranged on the combination pad; a detection line coated with an animal anti-human IgE polyclonal antibody and a quality control line coated with an animal IgG antibody are arranged on the reaction film. The invention further provides a preparation method, a kit and application of the detection card for quantitatively detecting the human total IgE antibody. The method can effectively eliminate the interference of non-specific fluorescence, greatly improves the analysis sensitivity, and is simple and convenient to operate and low in cost.

The present invention belongs to the technical field of antibody preparation, and in particular, relates to rabbit monoclonal antibodies, antibody conjugates and applications thereof against human PAX8 protein. The amino acid sequences of complementary determining regions CDR1-3 on the light chain variable region of the rabbit monoclonal antibody are shown in SEQ ID NO.3-5, respectively, and the amino acid sequences of complementary determining regions CDR1-3 on the heavy chain variable region are shown in SEQ ID NO.8-10, respectively. The anti-human PAX8 protein antibody of the present invention can specifically recognize the natural PAX8 protein on the surface of cells / tissues, and when used for immunohistochemical staining, the positive staining is obvious, the background is clean, and there is basically no non-specific staining, and the sensitivity and specificity can be as high as 100%, with the advantages of high specificity, good reliability, strong anti-interference ability, high analytical sensitivity and good detection accuracy, which is conducive to avoiding false positive and false negative results.

This invention discloses a wide dynamic range substrate reagent, belonging to the field of biological detection reagent technology. The reagent consists of the following components: citric acid, 9.6 g / L; 3,3',5,5'-tetramethylbenzidine, 0.29 g / L; 3% hydrogen peroxide solution, 3 mL / L; 1 g / L polyvinyl alcohol; 0.5 g / L copovidone W-735; 0.5 g / L dimethyldiallylammonium chloride-acrylamide copolymer; 1 g / L PVP K30; and Tween 20, 0.005 g / L. mL / L; polyethylene glycol 10g-50g / L; pure water, balance. This invention, by adding specific polyethylene glycol and synergistically combining with other components, significantly reduces the reagent background signal and improves detection sensitivity; effectively broadens the detection dynamic range and increases the detection upper limit of high-concentration analytes; improves the linear correlation between signal and concentration; and enhances the thermally accelerated stability of the reagent. This reagent has comprehensive performance and is especially suitable for ELISA detection systems with high sensitivity and wide detection range.

The invention relates to the technical field of clinical medical examination, in particular to an immunochromatography test strip for enhancing near-infrared two-region fluorescence through supramolecular host-guest interaction and a preparation method of the immunochromatography test strip. The invention provides a preparation method of an immunochromatographic test strip. The preparation method comprises the following steps: preparing a rare earth nanoparticle (RENPs) labeling solution; preparing a microsphere combination pad; preparing a sample pad; coating an antibody with a nitrocellulose membrane; and assembling and manufacturing the card. The aggregation phenomenon and partial fluorescence quenching effect caused in the surface modification process of the rare earth microspheres are reduced by utilizing an efficient surface modification strategy of a supramolecular host compound, the fluorescence luminescence property of the rare earth microspheres is effectively improved, and meanwhile, the antibody labeling efficiency of the rare earth microspheres is further improved by utilizing a supramolecular self-assembly strategy; the prepared test strip is obviously improved in analysis sensitivity, has the advantages of simplicity in operation, low cost and short detection time, and is suitable for rapid and sensitive detection of low-concentration markers in complex biological samples.

The invention discloses a high-throughput detection method for determining fructose-6-phosphoric acid in a cell matrix, a sample is introduced into a mass spectrum through sound wave ejection based on a sound wave excitation mass spectrum technology for target compound detection and analysis, a carrier liquid of the sample is an alkaline carrier liquid, the sample ejection amount of sound wave ejection is 10-15 nL, and the sample ejection amount of the sound wave ejection is 10-15 nL; the ejection frequency of sound wave ejection is that the interval between every two samples is 1000-4000 milliseconds. According to the detection method provided by the invention, the analysis time can be shortened, the manpower and consumable cost can be saved, the operation is simple and convenient, and the high-throughput detection requirement can be met.

The application discloses a mediator type integrated microbial electrode capable of detecting seawater biological toxicity, and belongs to the field of seawater biological toxicity detection. The electrode comprises an electrode base and a microbial membrane uniformly attached to the electrode base. The microbial membrane is a thin film obtained by fixing mixed and uniform electronic mediators and microorganisms on the electrode base through a gel solution and then drying. The gel solution is formed by mixing polydiallyldimethylammonium chloride solution and chitosan solution. The electrode can be used to quickly and accurately detect seawater biological toxicity, fills the blank of the mediator type integrated microbial electrode in the field of seawater toxicity evaluation, and opens up the application of the electrochemical method in marine water quality monitoring. The application further discloses a preparation method and application of the mediator type integrated microbial electrode.

The present invention provides a cilnidipine optical isomer impurity content detection method, which comprises: detecting cilnidipine optical isomer impurities by using a high performance liquid chromatography to obtain a liquid chromatogram, and calculating according to the liquid chromatogram to obtain the cilnidipine optical isomer impurity content, wherein a mobile phase used in the high performance liquid chromatography comprises methanol and water; a chromatographic column used in the high performance liquid chromatography is selected from Formes Speedcore PFP (Polyfluoropropylene). The method is high in detection efficiency and accuracy, good in impurity and principal component peak shape and capable of achieving baseline separation.

The invention discloses a reagent containing a porcine pseudorabies virus gB protein magnetic particle coating substance, a preparation method and a kit, and relates to the technical field of antibody detection. The preparation method of the reagent containing the porcine pseudorabies virus gB protein magnetic particle coating substance comprises the following steps: incubating magnetic particles and a gB protein antibody in a coating buffer solution; the reaction liquid is removed, and the gB protein magnetic particle coating and first confining liquid are subjected to first confining; after closing is completed, the first closing liquid is washed away, and the gB protein magnetic particle coating and second closing liquid are subjected to second-time closing; after closing is completed, the second closing liquid is washed away, resuspension is conducted, and the reagent containing the gB protein magnetic particle coating substance is obtained. The magnetic particle coating containing the porcine pseudorabies virus gB protein is prepared by adopting a two-step sealing method, the sealing effect is good, and non-specific adsorption in a system is reduced. The kit provided by the invention contains the reagents and has the advantages of high analysis sensitivity, wide linear range and high accuracy.

The application relates to a separation device and a VOC catalytic evaluation device comprising the same, and relates to the technical field of testing equipment. The separation device comprises a low-temperature separation tank and a secondary separator. The low-temperature separation tank is connected with the output end of a reaction furnace and is used for carrying out gas-liquid separation on the gas generated by the reaction furnace. The secondary separator is arranged in the low-temperature separation tank and is used for carrying out gas-liquid separation on the liquid generated by the low-temperature separation tank. The low-temperature separation tank comprises an outer shell, a first cooling member and a second cooling member arranged in the outer shell, an input pipeline with a first end located outside the outer shell and connected with the output end of the reaction furnace and a second end entering the outer shell and penetrating through the first cooling member, and an output pipeline with a first end located inside the outer shell and a second end penetrating through the second cooling member and extending out of the outer shell. The VOC catalytic evaluation device disclosed by the application reduces the influence of moisture on the detection result through active capture and supplementary detection of the moisture, so that more accurate detection results can be obtained.

The present invention provides a selective inhibitory cyclization auxiliary sequence and application. The selective inhibitory cyclization auxiliary sequence is composed of an interfering sequence binding region a, a linker binding region a, a linker binding region b and an interfering sequence binding region b from the 5' end to the 3' end, and the 5' end of the interfering sequence binding region a has a phosphate group; the interfering sequence specifically bound to the 5' end of the interfering sequence binding region a is adjacent to the interfering sequence specifically bound to the 3' end of the interfering sequence binding region b, and the interfering sequence is an interfering sequence in a fragment to be sequenced; the linker binding region a and the linker binding region b are respectively used for specific binding to a double-end linker sequence. The problem that a large number of sequencing results for uninteresting sequences or interfering sequences exist in the sequencing data in the prior art can be solved, and the method is suitable for the field of high-throughput sequencing.

This invention belongs to the field of power transmission capacity sensitivity analysis technology, specifically relating to a regional sensitivity analysis method for static maximum transmission capacity based on an improved 3D adaptive sparse grid interpolation method. The method includes: importing system parameters to obtain a deterministic MSTC calculation model; establishing a probabilistic model of uncertain sources in the power system and converting it into the required random input variables; correcting the boundaries of the random input variables to obtain a surrogate model for MSTC; generating MCS samples of random inputs to obtain the probability results of MSTC; calculating the CSM and CSV numerical results related to the random inputs of MSTC; selecting the top-ranking important random input variables; combining the corresponding CSM and CSV numerical results; analyzing the graphical expressions of the important random input variables; and determining adjustment strategies. This method efficiently identifies important random variables, thereby providing effective strategies for their adjustment and MSTC control, thus improving the stability and economic operation of the system.

The present invention provides a method and application for surface enhanced fluorescence, wherein a nano material is used as an enhanced substrate, a fluorescent substance to be tested is placed on the surface of the enhanced substrate, and then the fluorescent substance to be tested is stimulated by excitation light, and the multiple light scattering effect of the nano material on the excitation light and the radiated fluorescence is utilized to achieve enhancement of the fluorescence signal. According to the present invention, the problems existing in the existing surface enhanced fluorescence technology, such as large light loss, difficulty in control, complex process, high cost, etc., are solved, especially the problem of the current high dependence of the fluorescence enhancement substrate on metals and high refractive index materials is solved, and a lossless, non-metal-dependent surface enhanced fluorescence method is developed, which is not only suitable for the research of basic scientific problems, but also suitable for a wide range of practical applications.

The invention relates to the technical field of chemical analysis. The invention provides a method for determining various active components in scutellaria baicalensis based on UPLC-QTOF-MS. The technical method provided by the invention has the characteristics of rapidness and accuracy, and can qualitatively and quantitatively analyze the types and contents of the active components in the scutellaria baicalensis; the variety and the content of the active ingredients in the roots, the stems and the leaves of the scutellaria baicalensis and in the scutellaria baicalensis obtained at different harvesting times can be compared and analyzed, which is of great significance to the research on the subsequent quality control of the scutellaria baicalensis medicinal material.

The present invention relates to a method for performing a liquid phase immunoassay using a diagnostic test cartridge having a magnetic bead well, a reaction well, a washing well, and a measurement well. The liquid phase immunoassay method of the present invention includes a step of bringing a magnet close to a pipette tip to capture and transfer magnetic beads filled in the magnetic bead well to the reaction well, a step of providing a sample to the reaction well to perform an antigen-antibody reaction, a step of bringing the magnet close to the pipette tip to capture and transfer a reaction in the reaction well to the pipette tip and to the washing well to perform washing, and a step of bringing the magnet close to the pipette tip to transfer the reaction after washing in the washing well to the measurement well and to perform optical analysis. According to the present invention, the capture yield of magnetic beads can be improved.

The invention provides a detection method for ziprasidone synthesis intermediate 3-(1-piperazinyl)-1,2-benzisothiazole hydrochloride, belonging to the technical field of drug quality analysis and detection. The invention selects acetonitrile and triethylamine-containing aqueous solution in a certain proportion as mobile phases, performs programmed gradient elution in coordination, and improves separation degree and analytical sensitivity; a diode array detector is adopted, and the 2008-2022Shimadzu Corporation LabSolutions software / similar software carried by the diode array detector is used to confirm the relative quantification of main impurities in the main product 3-(1-piperazinyl)-1,2-benzisothiazole hydrochloride; and the method provided by the invention has short analysis time, simple analysis operation and good repeatability and adaptability.

The invention relates to the technical field of bioengineering, in particular to a recombinant human lipoprotein a and a preparation method of a polyclonal antibody thereof, which comprises the following steps: by taking pET28a (+) as an expression vector, introducing an Lp (a) gene of which the N-terminal is fused with six His tags, Trx tags and PP enzyme digestion sites under the control of a T7 promoter into escherichia coli for induction, denaturating and renaturating an inclusion body, purifying by a Ni Focurose 6FF column, and purifying by using a recombinant human lipoprotein a polyclonal antibody. The purity of the Lp (a) fusion protein is greater than or equal to 90%; the Lp (a) fusion protein is subjected to enzyme digestion and passes through a Ni Focurose 6FF column for purification, and the label-free Lp (a) protein with the purity larger than or equal to 95% is obtained; the Lp (a) polyclonal antibody is obtained by immunizing a goat with Lp (a) protein with the purity greater than or equal to 95%, collecting serum after each immunization, screening qualified serum through an antiserum bidirectional agar diffusion experiment, and purifying with Protein A. The Lp (a) prepared by the method is low in cost and relatively high in purity, and can be used as an effective immunogen for preparing the Lp (a) polyclonal antibody; and the material can be used as a calibrator raw material of a detection kit.

The invention discloses a method for synchronously measuring multiple endocrine disruptors in solid waste. The method comprises the following steps: collecting a solid waste sample, and refrigerating and storing the solid waste sample by adopting a colored sealed container; performing first vortex treatment on the solid waste sample by adopting a vortex reagent to obtain a to-be-extracted sample; performing ultrasonic extraction treatment on the to-be-extracted sample according to a first extraction condition to obtain a primary extraction product; determining a final object to be detected according to the turbidity condition of the primarily extracted product; setting chromatographic conditions and mass spectrum conditions, and performing HPLC-MS / MS measurement on the final to-be-measured substance based on the chromatographic conditions and the mass spectrum conditions to obtain content data of the 16 ED Cs in the solid waste sample; according to the method, ultrasonic extraction is combined with a high performance liquid chromatography-tandem mass spectrometry technology, related conditions are optimized, simple, convenient and rapid pretreatment steps are realized, and the analysis sensitivity, the recovery rate and the precision are improved.

The present invention provides a modified primer probe composition for specific detection of RNA, the modified primer probe composition comprising an upstream primer, a downstream primer and a probe; the upstream primer comprises a 5' end target gene sequence binding region, a complementary structure region, a loop structure region and a 3' end target gene sequence binding region. By inducing the structural conversion of the upstream primer through temperature changes at different stages of PCR, RNA is specifically captured to form cDNA under the condition of coexistence of host DNA and RNA; then cDNA is specifically identified and amplified to avoid interference of the sample's own DNA on amplification; at the same time, the lower limit of RNA detection is reduced and the analysis sensitivity is improved.

This invention discloses an ionizing emissive for iron isotope analysis and its preparation and testing methods. The ionizing emissive comprises a silicon nanoparticle suspension, a phosphoric acid solution, and an aluminum nitrate solution, wherein the phosphoric acid solution and aluminum nitrate solution are used as emissive excipients. In the iron isotope analysis testing method, the silicon nanoparticle suspension is used as a high-sensitivity emissive to enhance the ionization efficiency of the iron sample. Simultaneously, the phosphoric acid solution and aluminum nitrate solution assist ionization, and a high-purity rhenium filament is used as the sample carrier to test trace amounts of iron isotopes. The filament ionization temperature is 1350–1450℃. This invention has the advantages of high sensitivity, simple operation, and low cost. Compared with the traditional thermal ionization mass spectrometry method which requires 4000–50000 ng of sample, this invention only requires 400–4000 ng to obtain good iron isotope testing accuracy (<0.02%, RSE), significantly reducing the amount of iron sample used while improving testing sensitivity.

The invention belongs to the technical field of detection, and relates to a method for high-sensitivity quantification and structural identification of cobalamin and application thereof. The method comprises the following steps: by taking three common natural cobalamin with different structures in the environment as standard substances, obtaining cobalamin parent ions and high-abundance series characteristic ion fragments by adopting a mass spectrum acquisition mode of full scanning-automatic triggering secondary mass spectrum scanning; according to the principle that the same cobalamin fragments have the same fragment ion m / z and the fragment ion searching function of software, the purpose of identifying the unknown cobalamin structure is achieved; then, a mass spectrum quantitative method for parallel reaction monitoring is adopted, a large number of interference ions are filtered out through a quadrupole, cobalamin low ligand fragment ions with the highest abundance and the identification degree serve as quantitative ions, and the type and content of cobalamin in a to-be-measured sample are analyzed. By combining two data acquisition modes of high-resolution mass spectrometry, interference of an endogenous matrix can be effectively eliminated, the sample analysis sensitivity is improved, and the quantitative specificity is enhanced, so that high-sensitivity detection of different types of cobalamin is realized.