55results about How to "Low background signal" patented technology

The invention discloses a method suitable for simultaneously detecting 9 N-nitrosamines in food contact rubber products. The method comprises the following steps: utilizing a sample to be tested to establish a migration system to obtain a migration extract; utilizing the migration extract to prepare a sample solution to be tested to obtain a supernatant / filtrate; preparing 9 N-nitrosamines into a standard solution to further prepare a standard working solution with the gradient of 0.01-0.40 [mu]g / mL; injecting the gradient standard solution and the supernatant / filtrate into a liquid Chromatogram-tandem mass spectrograph, and testing the cation multi-reaction monitoring model to finally obtain the respective contents of 9 N-nitrosamines in the sample to be tested. The 9 N-nitrosamines are N-Nitrosodimethylamine, N-Nitroso-ethyl methylamine, N-Nitrosomorpholine, N-Nitrosopiperidine, N-Nitrosopyrrolidine, N-Nitroso-diethylamine, N-Nitrosodibutylamine, N-Nitrosodi-n-propylamine and N-Nitroso-diphenylamine.

The invention relates to an efficient cascade amplification antibody testing method which adopts the enzyme-linked immuno sorbent assay (ELISA). The method comprises the following steps: performing coating treatment, namely adsorbing the known antigen or antibody on the surface of solid phase carrier; adding specific second antibody labeled with enzyme to perform antigen antibody reaction on the surface of solid phase carrier; and adopting washing method to remove free components in liquid phase, wherein during the coating treatment, high purity immune serum is used as sealing agent. The method of the invention performs further improvements on the basis of ELISA, thus increasing the response signal, reducing the background signal and effectively amplifying the signal of a substance to be tested in the sample. Therefore, the method can be used to test the low-content substances or trace substances in the biological sample.

The invention discloses an antibody chip kit for early screening and diagnosis of tumors. The detection is realized based on an enzyme lined immunosorbent assay (ELISA); the antibody chip kit comprises an antibody chip and an antigen standard substance mixture of multiple tumor markers, wherein the antibody chip comprises a base membrane and capture antibodies, fixed on the base membrane, of the multiple tumor markers; the base membrane is a polydimethylsiloxane (PDMS) membrane having a surface containing hydrophilic groups. On the premise of maintaining the characteristics of high sensitivity, large flux, much information and the like, the base membrane adopted by the invention is good in stability, strong in biological compatibility, good in flexibility, easy to clean and convenient to use; chip substrates of the capture antibodies of the tumor markers are fixed.

A hsa-miR-629-5pdetection kit based on AllGlo probe fluorescent quantitative PCR (Polymerase Chain Reaction) and a detection method of the hsa-miR-629-5pdetection kit relate to MicroRNA. The detection kit is provided with a kit body, a clapboard, an exogenous reference bottle, a stem-loop reverse transcription reagent bottle, and a real-time fluorescent quantitative PCR reagent bottle. The miRNA in a sample is extracted, and if the sample is a serum / plasma sample or other liquid samples, then 5 mu L exogenous reference cel-miR-39 with the concentration of 5nmol, provided by the reagent bottle, is added after the sample is fully split, and the mixture is oscillated in vortex; but if the sample is a cell or tissue sample, then exogenous reference cel-miR-39 is not added; a stem-loop reverse transcription reagent provided by the reagent bottle is used to reversely transcribemiRNA into cDNA; a real-time fluorescent quantitative PCR reagent provided by the reagent bottle is used to conduct real-time PCR amplification; various data provided by an analytical instrument are integrated together, and reasonable threshold and reference line are set for result analysis.

The invention relates to single nucleotide polymorphism, in particular to a Glu504lys detection genotyping kit based on an AllGlo probe and a genotyping method thereof. The kit comprises a real-time fluorescence quantification PCR (polymerase chain reaction) reagent, positive control and negative control, Glu504lys is an SNP locus in an ALDH2 gene of human chromosome 12 provided by NCBI. The genotyping method comprises the steps that firstly, DNA in an EDTA anti-freezing whole blood sample is extracted through a conventional method; secondly, the real-time fluorescence quantification PCR reagent provided by the Glu504lys detection genotyping kit based on the probe AllGlo is used for conducting real-time fluorescence quantification PCR amplification on the DNA; and thirdly, genotyping is conducted on the Glu504lys locus of the human gene ALDH2 according to detected fluorescence signals. On the premise of keeping high specificity and sensitivity of the probe AllGlo, the detecting price is lower than that of a direct sequencing method, the process is simpler, and consumed time is shorter.

The invention discloses a CYP2C19*2 detection parting kit based on a probe AllGlo and a parting method of the CYP2C19*2 detection parting kit and relates to single nucleotide polymorphism (SNP). The kit comprises a real-time fluorescence quantification PCR reagent, positive control and negative control. CYP2C19*2 is an SNP locus in a CYP2C19 gene of human chromosome 10 provided by NCBI. The detection parting method comprises the steps that firstly, DNA in an EDTA anti-freezing whole blood sample is extracted through a conventional method; secondly, the real-time fluorescence quantification PCR reagent provided by the detection parting kit is used for conducting real-time fluorescence quantification PCR amplification on the DNA; thirdly, parting is conducted on the SNP CYP2C19*2 of the human gene CYP2C19 according to detected fluorescence signals. On the premise of keeping high specificity and sensitivity of the probe AllGlo, the detecting price is lower than that of a direct sequencing method, the process is simpler, and consumed time is shorter.

The present invention provides an oligonucleotide probe, which comprises an oligonucleotide molecule and a label bound to the oligonucleotide molecule, wherein the oligonucleotide molecule comprises: a hairpin capable of forming a hairpin structure A self-complementary region, a target nucleic acid recognition region, and a target nucleic acid recognition-like region, the label (a) providing a detectable signal when the probe is in a non-hybridizing form but having a reduced or substantially detectable signal when the probe hybridizes to a complementary nucleic acid Provides no detectable signal, or (b) provides a detectable signal when the probe hybridizes to a complementary nucleic acid, but provides a reduced or substantially no detectable signal when the probe is in non-hybridized form.

The invention discloses an hsa-miR-191-5p (Human Serum Albumin-Micro Ribonucleic Acid-191-5p) detection kit and an hsa-miR-191-5p detection method based on AllGlo probe fluorescent quantitative PCR (Polymerase Chain Reaction) and relates to MicroRNA (Micro Ribonucleic Acid). The kit is provided with a kit body, a partition, an exogenous reference bottle, a stem-loop reverse transcription reagent bottle and a real-time fluorescent quantitative PCR reagent bottle. The detection method comprises the steps of extracting miRNA from a sample, adding 5 microliters of exogenous reference cel-miR-39 (Caenorhabditis Elegans-Micro Ribonucleic Acid-39) at a concentration of 5n mol provided by the kit after full lysis of the sample, and swirling and shaking in case of a sample of serum / plasma sample or other body fluids, adding no exogenous reference cel-miR-39 in case of a sample of a cell or a tissue, performing reverse transcription on miRNA to form cDNA (Complementary Deoxyribonucleic Acid) with a stem-loop reverse transcription reagent provided by the kit, performing real-time PCR amplification on cDNA with a real-time fluorescent quantitative PCR reagent provided by the kit, and setting a reasonable threshold and a baseline for result analysis by synthesizing various data given by an analytical instrument.

The invention relates to microRNA, particularly an hsa-miR-708 detection kit based on AllGlo probe fluorescence quantitative PCR (polymerase chain reaction) and a detection method thereof. The kit is provided with a box body, partitions, an exogenous reference bottle, a stem-loop reverse transcription reagent bottle and a real-time fluorescence quantitative PCR reagent bottle. The method comprises the following steps: extracting miRNA (microribonucleic acid) in a sample, wherein if the sample is serum / plasma or any other body fluid, after the sample is sufficiently cracked, 5 mu L of 5n mol exogenous reference cel-miR-39 provided by the kit is added and vortex oscillation is performed, and if the sample is a cell or tissue sample, no exogenous reference cel-miR-39 is needed; carrying out real-time PCR amplification on the stem-loop reverse transcription reagent reverse transcription miRNA provided by the kit as cDNA (omplementary deoxyribonucleic acid) by using the real-time fluorescence quantitative PCR reagent provided by the kit; and setting reasonable thresholds and base lines according to data given by a comprehensive analysis instrument, and carrying out result analysis.

The invention discloses an hsa-miR-9 (Human Serum Albumin-Micro Ribonucleic Acid-9) detection kit and an hsa-miR-9 detection method based on AllGlo probe fluorescent quantitative PCR (Polymerase Chain Reaction) and relates to MicroRNA (Micro Ribonucleic Acid). The kit is provided with a kit body, a partition, an exogenous reference bottle, a stem-loop reverse transcription reagent bottle and a real-time fluorescent quantitative PCR reagent bottle. The detection method comprises the steps of extracting miRNA from a sample, adding 5 microliters of exogenous reference cel-miR-39 (Caenorhabditis Elegans-Micro Ribonucleic Acid-39) at a concentration of 5n mol provided by the kit after full lysis of the sample, and swirling and shaking in case of a sample of serum / plasma sample or other body fluids, adding no exogenous reference cel-miR-39 in case of a sample of a cell or a tissue, performing reverse transcription on miRNA to form cDNA (Complementary Deoxyribonucleic Acid) with a stem-loop reverse transcription reagent provided by the kit, performing real-time PCR amplification on cDNA with a real-time fluorescent quantitative PCR reagent provided by the kit, and setting a reasonable threshold and a baseline for result analysis by synthesizing various data given by an analytical instrument.

The invention discloses an hsa-miR-132 (Human Serum Albumin-Micro Ribonucleic Acid-132) detection kit and an hsa-miR-132 detection method based on AllGlo probe fluorescent quantitative PCR (Polymerase Chain Reaction) and relates to MicroRNA (Micro Ribonucleic Acid). The kit is provided with a kit body, a partition, an exogenous reference bottle, a stem-loop reverse transcription reagent bottle and a real-time fluorescent quantitative PCR reagent bottle. The detection method comprises the steps of extracting miRNA from a sample, adding 5 microliters of exogenous reference cel-miR-39 (Caenorhabditis Elegans-Micro Ribonucleic Acid-39) at a concentration of 5n mol provided by the kit after full lysis of the sample, and swirling and shaking in case of a sample of serum / plasma sample or other body fluids, adding no exogenous reference cel-miR-39 in case of a sample of a cell or a tissue, performing reverse transcription on miRNA to form cDNA (Complementary Deoxyribonucleic Acid) with a stem-loop reverse transcription reagent provided by the kit, performing real-time PCR amplification on cDNA with a real-time fluorescent quantitative PCR reagent provided by the kit, and setting a reasonable threshold and a baseline for result analysis by synthesizing various data given by an analytical instrument.

The invention relates to microRNA, particularly an hsa-miR-363 detection kit based on AllGlo probe fluorescence quantitative PCR (polymerase chain reaction) and a detection method thereof. The kit is provided with a box body, partitions, an exogenous reference bottle, a stem-loop reverse transcription reagent bottle and a real-time fluorescence quantitative PCR reagent bottle. The method comprises the following steps: extracting miRNA (microribonucleic acid) in a sample, wherein if the sample is serum / plasma or any other body fluid, after the sample is sufficiently cracked, 5 mu L of 5n mol exogenous reference cel-miR-39 provided by the kit is added and vortex oscillation is performed, and if the sample is a cell or tissue sample, no exogenous reference cel-miR-39 is needed; carrying out real-time PCR amplification on the stem-loop reverse transcription reagent reverse transcription miRNA provided by the kit as cDNA (omplementary deoxyribonucleic acid) by using the real-time fluorescence quantitative PCR reagent provided by the kit; and setting reasonable thresholds and base lines according to data given by a comprehensive analysis instrument, and carrying out result analysis.

The invention discloses a liquid mass spectrometry detection method for textile salicylate anti-ultraviolet finishing agents, which comprises the following steps: preparing a sample to be tested into a sample solution to be tested; The gradient standard working solution is prepared into a gradient standard working solution; the gradient standard working solution is injected into the liquid chromatography-tandem mass spectrometer, the positive and negative ion multiple reaction monitoring mode is measured, and the standard curve equation is made; the sample solution to be tested is detected according to the above method, so as to obtain the Contents of 7 kinds of salicylate anti-ultraviolet finishing agents in samples. The liquid chromatography-tandem mass spectrometry method is qualitative, quantitative, and highly sensitive, and is suitable for the detection of salicylate UV-resistant finishing agents in textiles.

The invention relates to microRNA, particularly an hsa-miR-520a-3p detection kit based on AllGlo probe fluorescence quantitative PCR (polymerase chain reaction) and a detection method thereof. The kit is provided with a box body, partitions, an exogenous reference bottle, a stem-loop reverse transcription reagent bottle and a real-time fluorescence quantitative PCR reagent bottle. The method comprises the following steps: extracting miRNA (microribonucleic acid) in a sample, wherein if the sample is serum / plasma or any other body fluid, after the sample is sufficiently cracked, 5 mu L of 5n mol exogenous reference cel-miR-39 provided by the kit is added and vortex oscillation is performed, and if the sample is a cell or tissue sample, no exogenous reference cel-miR-39 is needed; carrying out real-time PCR amplification on the stem-loop reverse transcription reagent reverse transcription miRNA provided by the kit as cDNA (omplementary deoxyribonucleic acid) by using the real-time fluorescence quantitative PCR reagent provided by the kit; and setting reasonable thresholds and base lines according to data given by a comprehensive analysis instrument, and carrying out result analysis.

The invention provides an hsa-miR-26a-2 detection kit based on fluorescent quantitative PCR (Polymerase Chain Reaction) of an AllGlo probe and a detection method thereof, and relates to a MicroRNA (Micro Ribose Nucleic Acid). The kit comprises a box body, a separator, an exogenous reference bottle, a stem-loop reverse transcription reagent bottle and a real-time quantitative PCR reagent bottle; the miRNA in a sample is extracted; if the sample is serum / plasma or one of other body fluid samples, 5 microliters of exogenous reference cel-miR-39 having the concentration of 5nmol provided by the kit is added after the sample is sufficiently crack, and then shaken in vortexes; if the sample is a cell or tissue sample, the exogenous reference cel-miR-39 does not need to be added; a stem-loop reverse transcription reagent provided by the kit is used for reversely transcribing the miRNA or a cDNA (complementary Desoxvribose Nucleic Acid); a real-time quantitative PCR reagent provided by the kit is used for real-time PCR amplification on the cDNA; various pieces of data provided by an instrument are analyzed comprehensively, rational thresholds and base lines are set rationally, and the results are analyzed.

The invention discloses an hsa-miR-146 (Human Serum Albumin-Micro Ribonucleic Acid-146) detection kit and an hsa-miR-146 detection method based on AllGlo probe fluorescent quantitative PCR (Polymerase Chain Reaction) and relates to MicroRNA (Micro Ribonucleic Acid). The kit is provided with a kit body, a partition, an exogenous reference bottle, a stem-loop reverse transcription reagent bottle and a real-time fluorescent quantitative PCR reagent bottle. The detection method comprises the steps of extracting miRNA from a sample, adding 5 microliters of exogenous reference cel-miR-39 (Caenorhabditis Elegans-Micro Ribonucleic Acid-39) at a concentration of 5n mol provided by the kit after full lysis of the sample, and swirling and shaking in case of a sample of serum / plasma sample or other body fluids, adding no exogenous reference cel-miR-39 in case of a sample of a cell or a tissue, performing reverse transcription on miRNA to form cDNA (Complementary Deoxyribonucleic Acid) with a stem-loop reverse transcription reagent provided by the kit, performing real-time PCR amplification on cDNA with a real-time fluorescent quantitative PCR reagent provided by the kit, and setting a reasonable threshold and a baseline for result analysis by synthesizing various data given by an analytical instrument.

A hsa-miR-629-5pdetection kit based on AllGlo probe fluorescent quantitative PCR (Polymerase Chain Reaction) and a detection method of the hsa-miR-629-5pdetection kit relate to MicroRNA. The detection kit is provided with a kit body, a clapboard, an exogenous reference bottle, a stem-loop reverse transcription reagent bottle, and a real-time fluorescent quantitative PCR reagent bottle. The miRNA in a sample is extracted, and if the sample is a serum / plasma sample or other liquid samples, then 5 mu L exogenous reference cel-miR-39 with the concentration of 5nmol, provided by the reagent bottle, is added after the sample is fully split, and the mixture is oscillated in vortex; but if the sample is a cell or tissue sample, then exogenous reference cel-miR-39 is not added; a stem-loop reverse transcription reagent provided by the reagent bottle is used to reversely transcribemiRNA into cDNA; a real-time fluorescent quantitative PCR reagent provided by the reagent bottle is used to conduct real-time PCR amplification; various data provided by an analytical instrument are integrated together, and reasonable threshold and reference line are set for result analysis.

The invention discloses a method suitable for simultaneously detecting 9 N-nitrosamines in food contact rubber products. The method comprises the following steps: utilizing a sample to be tested to establish a migration system to obtain a migration extract; utilizing the migration extract to prepare a sample solution to be tested to obtain a supernatant / filtrate; preparing 9 N-nitrosamines into a standard solution to further prepare a standard working solution with the gradient of 0.01-0.40 [mu]g / mL; injecting the gradient standard solution and the supernatant / filtrate into a liquid Chromatogram-tandem mass spectrograph, and testing the cation multi-reaction monitoring model to finally obtain the respective contents of 9 N-nitrosamines in the sample to be tested. The 9 N-nitrosamines are N-Nitrosodimethylamine, N-Nitroso-ethyl methylamine, N-Nitrosomorpholine, N-Nitrosopiperidine, N-Nitrosopyrrolidine, N-Nitroso-diethylamine, N-Nitrosodibutylamine, N-Nitrosodi-n-propylamine and N-Nitroso-diphenylamine.

The invention discloses an hsa-miR-513b (Human Serum Albumin-Micro Ribonucleic Acid-513b) detection kit and an hsa-miR-513b detection method based on AllGlo probe fluorescent quantitative PCR (Polymerase Chain Reaction) and relates to MicroRNA (Micro Ribonucleic Acid). The kit is provided with a kit body, a partition, an exogenous reference bottle, a stem-loop reverse transcription reagent bottle and a real-time fluorescent quantitative PCR reagent bottle. The detection method comprises the steps of extracting miRNA from a sample, adding 5 microliters of exogenous reference cel-miR-39 (Caenorhabditis Elegans-Micro Ribonucleic Acid-39) at a concentration of 5n mol provided by the kit after full lysis of the sample, and swirling and shaking in case of a sample of serum / plasma sample or other body fluids, adding no exogenous reference cel-miR-39 in case of a sample of a cell or a tissue, performing reverse transcription on miRNA to form cDNA (Complementary Deoxyribonucleic Acid) with a stem-loop reverse transcription reagent provided by the kit, performing real-time PCR amplification on cDNA with a real-time fluorescent quantitative PCR reagent provided by the kit, and setting a reasonable threshold and a baseline for result analysis by synthesizing various data given by an analytical instrument.

The invention discloses an hsa-miR-137 (Human Serum Albumin-Micro Ribonucleic Acid-137) detection kit and an hsa-miR-137 detection method based on AllGlo probe fluorescent quantitative PCR (Polymerase Chain Reaction) and relates to MicroRNA (Micro Ribonucleic Acid). The kit is provided with a kit body, a partition, an exogenous reference bottle, a stem-loop reverse transcription reagent bottle and a real-time fluorescent quantitative PCR reagent bottle. The detection method comprises the steps of extracting miRNA from a sample, adding 5 microliters of exogenous reference cel-miR-39 (Caenorhabditis Elegans-Micro Ribonucleic Acid-39) at a concentration of 5n mol provided by the kit after full lysis of the sample, and swirling and shaking in case of a sample of serum / plasma sample or other body fluids, adding no exogenous reference cel-miR-39 in case of a sample of a cell or a tissue, performing reverse transcription on miRNA to form cDNA (Complementary Deoxyribonucleic Acid) with a stem-loop reverse transcription reagent provided by the kit, performing real-time PCR amplification on cDNA with a real-time fluorescent quantitative PCR reagent provided by the kit, and setting a reasonable threshold and a baseline for result analysis by synthesizing various data given by an analytical instrument.