56results about How to "Low background signal" patented technology

This invention discloses a wide dynamic range substrate reagent, belonging to the field of biological detection reagent technology. The reagent consists of the following components: citric acid, 9.6 g / L; 3,3',5,5'-tetramethylbenzidine, 0.29 g / L; 3% hydrogen peroxide solution, 3 mL / L; 1 g / L polyvinyl alcohol; 0.5 g / L copovidone W-735; 0.5 g / L dimethyldiallylammonium chloride-acrylamide copolymer; 1 g / L PVP K30; and Tween 20, 0.005 g / L. mL / L; polyethylene glycol 10g-50g / L; pure water, balance. This invention, by adding specific polyethylene glycol and synergistically combining with other components, significantly reduces the reagent background signal and improves detection sensitivity; effectively broadens the detection dynamic range and increases the detection upper limit of high-concentration analytes; improves the linear correlation between signal and concentration; and enhances the thermally accelerated stability of the reagent. This reagent has comprehensive performance and is especially suitable for ELISA detection systems with high sensitivity and wide detection range.