The invention discloses a space metabonomics
analysis method and application thereof, relates to the technical field of metabonomics analysis, and solves the problem that an analysis result of an existing conventional space metabonomics
analysis method is not accurate enough. To-be-analyzed
biological tissue samples are taken and divided into groups; preparing all groups of samples into slices, adsorbing the slices on the same
glass slide, carrying out
mass spectrum imaging detection, and synchronously collecting
metabolite spatial distribution signals and
mass spectrum intensity data of all the samples; the method comprises the following steps: preprocessing collected
original data, identifying the distribution condition of known exogenous components in a
medicine in a tissue slice, and delimiting a region with
high signal intensity as a
region of interest; randomly selecting a plurality of pixel points in the
region of interest of each sample; and integrating the
mass spectrum data corresponding to the pixel points selected from all the parallel samples in the same group, and sequentially carrying out PCA, OPLS-DA, differential
metabolite screening and KEGG pathway enrichment analysis. The method can be applied to research on the hepatotoxicity of the
polygonum multiflorum and the prepared
polygonum multiflorum.