Efficient cascade amplification antibody testing method

Abstract

The invention relates to an efficient cascade amplification antibody testing method which adopts the enzyme-linked immuno sorbent assay (ELISA). The method comprises the following steps: performing coating treatment, namely adsorbing the known antigen or antibody on the surface of solid phase carrier; adding specific second antibody labeled with enzyme to perform antigen antibody reaction on the surface of solid phase carrier; and adopting washing method to remove free components in liquid phase, wherein during the coating treatment, high purity immune serum is used as sealing agent. The method of the invention performs further improvements on the basis of ELISA, thus increasing the response signal, reducing the background signal and effectively amplifying the signal of a substance to be tested in the sample. Therefore, the method can be used to test the low-content substances or trace substances in the biological sample.

Description

technical field [0001] The invention relates to the technical field of diagnostic immune analysis, in particular to an antibody detection method with high-efficiency cascade amplification. Background technique [0002] Detecting low or even trace amounts of substances in biological samples is one of the challenging tasks of in vitro diagnostic reagents. Generally speaking, for the higher content of antigenic substances in the sample, such as hCG in urine of pregnant women or specific IgG in serum, the routine detection method is enzyme-linked immunosorbent assay (ELISA) or similar deformations based on this method method (such as Western blotting). However, when detecting low or trace amounts of antigenic substances, conventional ELISA generally has the problems of low signal, high background, and unstable test results, so that the results are negative or weakly positive, so it is of great significance for clinical diagnosis. not big. [0003] It can be seen that the abov...

AI Technical Summary

Problems solved by technology

However, when detecting low or trace amounts of antigenic substances, conventional ELISA generally has the problems of low signal, high background, and unstable test results, so that the results are negative or weakly positive, so it is of great significance for clinical diagnosis. not big

[0003] It can be seen that the above-mentioned existing antibody detection methods obviously still have inconvenience and defects in the application of low-volume or trace-quantity detection, and need to be further improved urgently.

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