291results about How to "Enhanced detection signal" patented technology

A method of analysing a nucleic acid is disclosed which involves the use of a mixture of labelled oligonucleotides in solution and an array of immobilized oligonucleotides. The target nucleic acid is incubated with the mixture of labelled oligonucleotides. Those labelled oligonucleotides which hybridize are recovered and incubated with the array of immobilized oligonucleotides. Sequence information is obtained by observing the location of the label on the array. The method is particularly suitable for determining differences between nucleic acids.

The present invention relates to a microfluidic electrochemical sensor apparatus and a method for conducting analytical tests with said apparatus for multi-reactant assays. The apparatus of this invention is a multi-layer body made of at least three layers, the first one being a polymer layer (1) comprising a microstructure (5) with at least one integrated microelectrode (4) and conductive tracks (13) for connection to an external electrochemical unit, the second one being a non-porous material serving to cover said microstructure so as to enable microfluidic manipulations and the third one being a porous layer (2) such as a membrane or a glass frit, said porous layer comprising at least one reagent (3) to be solubilized upon contact with a test solution (7) and reacting with an analyte (6) present in said solution to form a product that is transported along said microstructure so as to enable electrochemical detection of said analyte. The invention notably enables the performance of multi-reactant assays in a reduced number of steps.

The present invention discloses analyte detection devices for detecting one or more analytes present in test samples, especially biological samples. In particular, the devices of the invention are lateral flow assay devices comprising immobilized metal nanoparticle conjugates as the detection means. Methods of using the devices and kits comprising the devices are also described.

Compositions and related methods for detecting leaks in HVAC / R components such as furnaces, portable gas heaters, swimming pool heaters, air conditioners, heat pumps, refrigerators, freezers, and other closed loop vapor compression systems, as well as heat exchangers. These compositions provide more sensitive detection of leaks in inaccessible areas of furnaces using low ODP and non-flammable compounds classified as hydrofluorochloro-ethers (HFCE's), hydrobromochlorofluoro-alkenes (HBCFA's) hydrofluoro-ethers (HFE's), and halogenated compounds which are either alkanes, alcohols, diones, acetates, ketones (e.g., butanones, pentanones), esters (e.g., propanoates), anhydrides, cycloalkanes (cycloparaffins), cycloalkenes (cycloolefins), heterocyclics (e.g., furans), and aromatics.

The present invention provides oligonucleotides and methods for efficient detection of target nucleic acids using rolling circle replication. In one aspect, the oligonucleotides of the invention are characteristic in that they can be circularised without an external ligation template. In another aspect, the oligonucleotides of the invention are characteristic in that they can generate a free 3′end of the target nucleic acid necessary for rolling circle replication. The oligonucleotides and detection methods of the invention are useful e.g. as research tool and for diagnosis.

The invention provides a probe based on double-signal amplification triggered by a target and application of the probe. The probe comprises a first hairpin, a second hairpin and a third hairpin, wherein the first hairpin, the second hairpin and the third hairpin are formed by pairing hybridization of complementary bases in folding areas after single-chain linear molecules are folded back; the parts, forming double-chain structures in the partial areas, of the hairpins are stem areas, and the parts, not forming the double-chain structures and folded back, are ring-shaped areas. According to the probe provided by the invention, the self-assembly of hairpins is realized through target protein catalysis, signal amplification is achieved under the assistance of an excision enzyme III, obvious amplification of a detection signal is realized, and the detection sensitivity is greatly improved further; since the probe does not depend on template replication in the detection process, the problem that false positive occurs due to cross contamination in the detection process is avoided, generation of a false positive signal is effectively prevented and background noise is reduced greatly.

The invention discloses a preparation method for a small-molecule microarray. The preparation method comprises the steps of bonding the hydroxyl terminal of a nonspecific adsorption resistance material modified on a chip substrate with the hydroxyl terminal of a small-molecule light crosslinking agent through an esterification reaction, and randomly fixing small molecules on the hydroxyl terminals through light crosslinking. By the adoption of a direct esterification mode, the small-molecule light crosslinking agent is quickly and efficiently coupled to the surface of the nonspecific adsorption resistance material; a chip modification process is simple and convenient and can be implemented under a normal temperature condition; therefore, the modification cost is reduced, and the modification efficiency is improved; a high detection signal is guaranteed; a non-reacted group still keeps high nonspecific adsorption resistance capacity, so that a background signal is reduced; the prepared small-molecule microarray is high in detection signal and low in background signal and can be used for mutual action between high-flux screened small-molecule medicaments and targets.

Methods and apparatus are provided for detection of microorganisms in a sample. Methods and apparatus of the invention are based on the specificity that phage, for example bacteriophage, have for target microorganisms, for example bacterium. Typically, phage adsorption to target microorganisms act as signal, or a signal target, for the presence of the target microorganism. Typically, the phage are labeled with a detectable signal. Apparatus of the invention are directed toward concentrating the phage adsorbed microorganisms at a predetermined site for flag dependent observation.

The present invention relates to the technical field of diagnostic reagents, and particularly relates to a magnetic immunization in-vitro diagnostic reagent. In order to solve the technical problems of smaller capacity of an immobilized antibody, long reaction time, relatively low sensitivity and low stability of the immobilized antibody of a conventional enzyme immunoassay, the present invention provides a GP73 monoclonal antibody and a superparamagnetic nano immunization microsphere with the GP73 monoclonal antibody coupled with the surface, and a human serum or plasma GP73 antigen detection method using the superparamagnetic nano immunization microsphere by double-antibody sandwich enzyme immunoassay or chemiluminescence method. An antigen for producing the GP73 monoclonal antibody has the following amino acid sequence AAAERGAVELKK. The superparamagnetic nano immunization microsphere has the characteristics of being capable of coupling more antibodies, fast in immunoreaction speed, high in specificity, good in repeatability, low in cost, and simple in experimental condition requirements and the like.

The invention provides a micro-fluidic SERS chip for nondestructive testing of blood and a biological sample. The chip is in a 'sandwich' structure which is formed by a substrate, a PDMS interlayer and a cover plate, and comprises a micro-channel, a sampling opening and a discharge opening, wherein a plurality of detection holes are formed in the middle of the micro-channel to form a porous detection zone. A dual-layer nano SERS enhancement base which can be repeatedly used is prepared in an in-situ manner by combination of silver mirror reaction and a chemical plating self-assembly method, so that the SERS detection sensitivity and the detection efficiency are effectively improved. The sampling opening is designed into a conical end sampling opening to form a capillary sampling mode; a to-be-detected liquid is directly drained to a to-be-detected zone by the capillary action; the effects caused by the dripping process are avoided; the chip comprises a plurality of detection zones, so that once sampling and multi-time detection can be achieved; and high detection repeatability is improved by determining the average value. The chip is relatively small in size, and convenient to carry, and can be used as a detection tool which is carried by a tester, and is relatively simple in preparation method, low in cost and suitable for multi-point parallel testing of the biological sample.

The invention discloses a pipeline water conveying system multi-point tiny leakage detection device and method. The method comprises the steps that transient drive signal generation equipment and a single-point pressure sensing unit are arranged at the tail end of a pipeline of a detected pipe net system to monitor tiny leakage points between the pipeline upstream position and the motivation position; the transient drive signal generation equipment is started, short-time drive signals are generated, and the single-point pressure sensing unit synchronously collects transient pressure signals; the transient pressure signals are analyzed, and the time and the reduced amplitude corresponding to pressure sudden change points of the first-phase pressure wave of the signals are analyzed; and the number of leakage points is identified according to the number of the pressure sudden change points of the first-phase pressure wave; and the position and leakage parameter of each leakage point are calculated according to the time and the reduced amplitude corresponding to each pressure sudden change point. The fact that the attenuation and distortion rule of the first pressure wave of transient water attack is different from that of single-hole leakage is utilized, multi-point and tiny leakage identification is conducted, simpleness and effectiveness are achieved, understanding is easy, the collected data volume is small, and reliability is high.

The invention discloses a pulse eddy electromagnetism nondestructive detection method based on a probe with an iron core coil. The probe consists of an iron core bundle, an excitation coil and a detection coil, wherein the iron core bundle is arranged in the center of the excitation coil; the detection coil is arranged outside the excitation coil. When the probe with the iron core coil is adopted to perform pulse eddy detection on a part, a strong pulse magnetic field of a dense space can be generated, an excitation magnetic field is increased, the signal to noise ratio of a signal is increased, and pulse eddy detection on a component with a relatively large wall thickness can be achieved under a relatively high lift-off condition. When a pulse eddy electromagnetism nondestructive detection system based on the probe with the iron core coil is adopted for detection, the influence of the pulse eddy field inside the iron core to the detection signal can be measured according to a probe time constant tau s. When tau s is greatly smaller than a part diffusion time constant tau e, the influence of the pulse eddy field inside the iron core to the detection signal can be ignored, the function of the probe with the iron core coil can be approximated to be linear amplification of the detection signal, and thus the signal processing difficulty is reduced.

This invention allows ultra-low levels of virtually any biological analyte to be detected and quantified rapidly, simply and inexpensively with an electrochemical biosensor using a novel electrochemical signal amplification technique. The invention amplifies detection signals from low level analytes using an innovative sandwich ELISA structure that replaces optical labels with a massive amount of electrochemically detectable guanine rich oligonucleotide tags. Selective binding is achieved with matched pairs of either commercial or custom analyte binding materials such as monoclonal antibodies or single stand DNA. The guanine tags are eluted from the sandwich structures and hybridize with complementary cytosine rich oligonucleotide recognition probes attached to the surface of a biosensor working electrode. An electrochemical technique generates a signal in proportion to the guanine level on the working electrode which is also proportional to the analyte level in the sample. Magnetic separation and a nanosensor are used to improve the signal-to-noise ratio for measuring analyte levels 1,000,000 times lower than enzyme-linked immunosorbent assay (ELISA).

A photoelectric converting layer lamination type solid-state image pick-up device comprises: a semiconductor substrate; at least three layers of photoelectric converting layers each of which is interposed between a common electrode layer and pixel electrode layers, the pixel electrode layers corresponding to pixels respectively, wherein said at least three layers of photoelectric converting layers are laminated through insulating layers, said at least three layers of photoelectric converting layers being above the semiconductor substrate, and wherein each of said at least three layers of photoelectric converting layers is separated into layers corresponding the pixels respectively.

The invention discloses a liquid phase chip for detecting breast cancer prognosis-related gene mRNA expression level, which mainly comprises a microsphere which aims at different target genes and is coupled to amido modified supporting probes, supporting extended probes connecting the supporting probes and target gene mRNA and amplification extended probes. Each supporting probe mainly comprises a 5' end spacer arm sequence and a 3' end specific sequence P1 which is in complementary pairing with a supporting extended probe; the supporting probe comprises SEQ. ID NO.1 specific to ESR1gene and SEQ. ID NO.2 specific to PGR gene; each supporting extended probe mainly comprises a 5' end specific sequence P2 which can be correspondingly combined with the target genes, a spacer arm sequence and a 3' end specific sequence P3 which is in complementary pairing with the specific sequence P1 of a corresponding supporting probe; and the supporting extended probes comprise amplification extended probes, wherein each amplification extension probe comprises a 5' end specific sequence P4 which can be combined with the target genes, a spacer arm sequence and a 3' end sequence P5; or the liquid phase chip also comprises a labeling probe in complementary pairing with the sequence P5. The liquid phase chip can perform hybridization reaction under the homogeneous reaction condition, and the designed probes have the advantages of high specificity and high signal to noise ratio during detection.

An active electronic tag and a signal modulation method are provided herein. The active electronic tag includes an antenna, a first amplifier, an automatic gain control circuit, a phase synchronization locking circuit, a control unit, a tag unit and a second amplifier. The antenna receives a detecting signal transmitted from a reader, in which the detecting signal is carried on a first carrier. The phase synchronization locking circuit generates a second carrier. The control unit generates a response signal, in which the response signal is carried on the second carrier. By utilizing a phase difference of the first carrier and the second carrier, the response signal cancels the first carrier on the reader so as to transmit the information stored in the active electronic tag.

The invention relates to an electrochemical luminescence adapter sensor as well as a preparation method and the usage thereof, and in particular relates to an electrochemical luminescence adapter sensor established by taking Eu (III)-MWCNTs as luminescent material, a preparation method of the electrochemical luminescence adapter sensor, and the usage of the electrochemical luminescence adapter sensor prepared by the method in measurement of thrombin. The electrochemical luminescence adapter sensor has the advantages of excellent accuracy, stability and reproducibility and high sensitivity and has an important significance in the popularization of practical application of the adapter sensor in disease prevention, disease diagnosis and drug screening.

The invention discloses a Fe-Ga material based magnetostriction liquid-level sensor. The liquid-level sensor is composed of a sensor head, a measuring rod and a floater, the sensor head is connected with the measuring rod in a threaded manner, and the moving floater sleeves the lower portion of the measuring rod. The sensor head is of a cylindrical structure, and a hardware circuit system, a detecting device and a front damping device are arranged in the sensor head. A Waveguide wire is made of Fe-Ga materials and arranged in the measuring rod. The front end of the waveguide wire enters the sensor head through a threaded hole and sequentially penetrates a coil frame in the detecting device and the center of the front damping device till to the hardware circuit system. The tail end of the waveguide wire penetrates the center of a rear damping device to be connected with a return lead, the return lead is connected to a hardware circuit to form a closed loop. The Fe-Ga alloy is used as materials of the waveguide wire of the magnetostriction liquid-level sensor and can be applied to wide-range liquid-level sensors, and the Fe-Ga material based magnetostriction liquid-level sensor can stably work in the severe environment.

The invention discloses a magnetic particulate chemiluminescent micro-fluidic chip for quantitatively detecting myohemoglobin. The structure of the magnetic particulate chemiluminescent micro-fluidic chip mainly comprises a cover piece (1) and a bottom piece (11), wherein an air pump (3), an airflow micro-channel (5), a sample feeding hole (2), a sample liquid flowing channel (6), a first biological marker storage tank (4), a micro-mixer (7) and a transition region (10) on the cover piece (1) are connected in sequence; a filter (12), a reaction tank (13), a washing tank (14), a detection tank (15) and a solution releasing channel (18) on the bottom piece (11) are connected in sequence; the detection tank (15) on the bottom piece (11) is connected with a washing liquid storage tank (16) and a luminescent liquid storage tank (17) through the solution releasing channel (18).

Methods and apparatus are provided for detection of microorganisms in a sample. Methods and apparatus of the invention are based on the specificity that phage, for example bacteriophage, have for target microorganisms, for example bacterium. Typically, phage adsorption to target microorganisms act as signal, or a signal target, for the presence of the target microorganism. Typically, the phage are labeled with a detectable signal. Apparatus of the invention are directed toward concentrating the phage adsorbed microorganisms at a predetermined site for flag dependent observation.

The invention relates to a coding sequence of anti-Cry1Ac scFv and an immuno-PCR detection method for detecting Cry1Ac through the phage scFv. The scFv are obtained through elutriation in a natural phage antibody base according to the method. The method is characterized in that the specificity of the scFv is determined by a CDR region sequence in a heavy chain and light chain variable region sequence, immuno-PCR results indicate that a fluorescence detection system can be used for quantitative detection of the Cry1Ac, and the detection range is 0.2-100ng / mL. The method is fast, simple and high in flexibility; a step for marking reporter DNA molecules during an existing immuno-PCR process is avoided, the experimental operation is simplified, and potential practical values are provided.

This application relates to methods and apparatus for fiber optic sensing which can provide information about the environment in which the fiber optic is deployed. In particular the application relates to fiber optic based sensing of the mechanical impedance of the environment. The method comprises using an interrogator (201) to interrogate an optical fiber (104) which is coupled to a first element (202; 802) which is responsive to electromagnetic fields. In use a varying electric current (I), which may be an alternating current, is applied so as to induce a varying force (F) on said first element. The optical radiation backscattered from within the optical fiber is analyzed to determine a measurement signal indicative of a variation in the backscattered radiation corresponding with said electric current applied. The first element may be a first conductor (202) and the varying current may be supplied to the first conductor, or to a second conductor (701). Alternatively the first element could be a magnetic element (802). By applying a variable force to the first element, and hence the optical fiber, the characteristics of the environment can be determined.

The present invention provides oligonucleotides and methods for efficient detection of target nucleic acids using rolling circle replication. In one aspect, the oligonucleotides of the invention are characteristic in that they can be circularised without an external ligation template. In another aspect, the oligonucleotides of the invention are characteristic in that they can generate a free 3′end of the target nucleic acid necessary for rolling circle replication. The oligonucleotides and detection methods of the invention are useful e.g. as research tool and for diagnosis.

The invention belongs to the technical field of nondestructive testing, and relates to a circular dual-frequency excitation eddy current probe and a method for detecting thick-wall deep crack defects.The circular dual-frequency excitation eddy current probe comprises dual-frequency excitation probe assemblies, a detection probe assembly and a fixing frame; two sets of excitation probe assembliesare provided, and are distributed in bilateral symmetry with the detection probe assembly as a center; each excitation probe assembly consists of an excitation coil and a excitation coil mounting vertical column; the detection probe assembly consists of a detection coil and a detection coil mounting vertical column; the fixing frame consists of a vertical column fixing plate and a scanning component connecting component which are fixedly connected; the bottom end of the excitation coil mounting vertical column and the bottom end of the detection coil mounting vertical column are mounted on thevertical column fixing plate; the excitation coil is a circular eddy current coil and is excited by adopting a sinusoidal dual-frequency AC current. After adoption of the circular dual-frequency excitation eddy current probe, the penetration depth of an eddy current and the deep crack detecting ability of the probe can be improved, and the penetration depth of the circular dual-frequency excitation eddy current probe can reach about 21mm.

The invention relates to a wafer detection method and a wafer detection apparatus. The method comprises enabling grazing incidence of two or more than two coherent light beams onto the to-be-measured wafer and forming interference fringes on the to-be-measured wafer; performing rotation and translation for the to-be-measured wafer so that the interference fringes scan the to-be-measured wafer; scattering the interference fringes by particles on the surface of the to-be-measured wafer and forming time-related scattered light signals; detecting the scattered light signals, treating the scattered light signals based on characteristic frequency corresponding to the particles at different positions of the to-be-measured wafer, and forming frequency-dependent detection information; and obtaining the distribution information of the particles on the to-be-measured wafer based on the detection information. The wafer detection method is high in precision and throughput; and the wafer detection apparatus is low in design difficulty and cost.

The invention relates to a capture probe set, method and kit for detecting pathogenic microorganisms and application, and belongs to the technical field of gene detection. The capture probe set specifically detects 62 bacteria, 41 drug-resistant genes, 16 fungi, 14 parasites, 15 DNA viruses and 60 RNA viruses, can cover 95% or above of pathogenic microorganisms with the high detection rate in clinical samples, can greatly improve the detection sensitivity and specificity by combining the current situation that part of pathogenic microorganisms are difficult to detect, and particularly improves the detection sensitivity of pathogenic microorganisms which are significant to clinic.

The invention discloses a magnetic particle chemiluminescence microfluidic chip for quantitative detection of a D-dimer. The structure of the chip mainly comprises a cover plate (1) and a base plate (11), wherein a sample introduction opening (2), a sample liquid flow channel (6), a first biomarker storage pool (4), a micro-mixer (7) and a transition zone (10) which are arranged on the cover plate (1) are sequentially connected; and a filter (12), a reaction pool (13), a cleaning pool (14), a detection pool (15) and a solution release channel (18) which are arranged on the base plate (11) are sequentially connected, and the detection pool (15) on the base plate (11) is connected with a cleaning liquid storage pool (16) and a luminescence liquid storage pool (17) through the solution release channel (18).

A method and apparatus is described for aligning a first article relative to a second article, for example for aligning a nanoimprint template with a semiconductor wafer. The method comprises the steps of: providing said second article with at least one flexible structure fixed relative thereto at least one point, providing a first article having at least one surface relief marking thereon, providing a detector for measuring an interaction of the flexible structure with the surface relief marking and generating detector signals relating to said interaction, identifying with the help of the detector signals the position of the flexible structure and thus of the second article with respect to the surface relief marking and generating relative movement between the first and second articles to achieve a desired alignment between the first and second articles defined by the surface relief marking. In this method and apparatus the flexible structure is brought into contact with the surface relief marking.

The invention provides preparation and application of a mercury ion sensor based on photonic crystal enhanced fluorescence. The mercury ion sensor is composed of photonic crystal, a nucleic acid fluorescence molecular probe and a DNA assistant probe. The photonic crystal is used as a substrate, and strong affinity between thymidine (T) of DNA and Hg<2+> is employed to allow fluorescence group ROX labeled aptamer and a complementary sequence to compose a fluorescence detection system. A photonic crystal film is formed on a glass sheet substrate by prepared PS microspheres through vertical deposition self-assembly. Gold is sprayed on the surface of the photonic crystal; and based on Au-S bonding effect, mercapto group modified fluorescence group ROX labeled aptamer undergoes self-assembly on the surface of the photonic crystal, wherein the emission wavelength of ROX accords with the forbidden band of the photonic crystal, so fluorescence enhancing effect is achieved. According to the invention, fluorescence signal intensity of a nucleic acid fluorescence molecule is improved by using the principles of enhancement of fluorescence through the photonic crystal, so sensitivity and accuracy of sensor detection are improved.

The invention provides a cystatin C latex-particle-enhanced turbidimetry detection reagent kit and application thereof.The reagent kit comprises a reagent R1 and a reagent R2.The reagent R1 is prepared from a buffer solution, inorganic salt, a surfactant, a preservative, a stabilizer and interference elimination protein.The reagent R2 is prepared from a buffer solution, inorganic salt, a surfactant, a preservative, a stabilizer and a polystyrene latex particle mixture, and the polystyrene latex particle mixture is interlinked with a cystatin C antibody.The reagent kit based on latex-particle-enhanced turbidimetric immunoassay (PETIA) can be generally applied to analysis of various full-automatic biochemical analyzers and is short in assay time, good in specificity, high in precision and good in accuracy when used.