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Micro-fluidic SERS chip for nondestructive testing of blood and biological sample

A biological sample, non-destructive testing technology, applied in the fields of biochemical testing, chips, and spectroscopy, can solve the problems of limited types of reinforced matrix materials, high preparation and processing costs, shorten the detection time, improve the detection limit, and achieve good results.

Active Publication Date: 2015-04-29
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former has the advantages of orderly and controllable nanoparticle size, but the types of reinforcing matrix materials are limited and the preparation and processing costs are high; the latter has the characteristics of easy expansion of matrix types, low processing costs, and flexible methods for combining with microfluidic channels.
In the preparation of SERS substrates by chemical self-assembly method, there are many researches and methods for preparing nano-gold and silver. However, such substrate structures still need to be improved in terms of SERS signal loss and detection sensitivity.

Method used

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  • Micro-fluidic SERS chip for nondestructive testing of blood and biological sample
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  • Micro-fluidic SERS chip for nondestructive testing of blood and biological sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The specific implementation steps of the microfluidic SERS chip preparation of the Ag / Au double metal layer SERS active substrate are as follows:

[0020] (1) Prepare a substrate, and closely bond the substrate with PDMS microchannels and PDMS films to form a "sandwich" sandwich chip structure;

[0021] (2) Coating an Ag film on the surface of the microchannel by using the silver mirror reaction;

[0022] (3) Assembling Au nanoparticles on the surface of Ag film by chemical self-assembly;

[0023] (4) In situ deposition on Au nanoparticles by electroless plating method, integrated preparation of Ag / Au double metal layer SERS active substrate.

[0024] In this embodiment, step (2) includes pretreatment of the glass substrate and silver mirror reaction.

[0025] Soak the glass substrate in hot NaOH solution and ultrasonically clean it with an ultrasonic cleaner for about 10 minutes to remove the oil on its surface. Then soak in fresh "piranha lotion" (98%H) at 80°C 2 ...

Embodiment 2

[0037] The SERS application detection was carried out on the SERS micro-detector in Example 1. Human whole blood was passed into the microfluidic detector by capillary sampling, using an inVia Raman spectrometer (Renishaw, France), the laser wavelength was 785nm, the laser power was 1%, the number of exposures was once, and the integration time was 5s. Amount of 1uL was tested for SERS activity, and the SERS spectrum of human whole blood was obtained ( figure 2 ) The two spectral lines are the spectra obtained with SERS substrate and without SERS substrate respectively. It can be seen from the spectra that the micro-detector can effectively identify blood, and the SERS substrate has a significant enhancement effect on the signal.

[0038] It can be seen that the substrate structure of the present invention has advantages in reducing SERS signal loss and improving detection sensitivity.

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Abstract

The invention provides a micro-fluidic SERS chip for nondestructive testing of blood and a biological sample. The chip is in a 'sandwich' structure which is formed by a substrate, a PDMS interlayer and a cover plate, and comprises a micro-channel, a sampling opening and a discharge opening, wherein a plurality of detection holes are formed in the middle of the micro-channel to form a porous detection zone. A dual-layer nano SERS enhancement base which can be repeatedly used is prepared in an in-situ manner by combination of silver mirror reaction and a chemical plating self-assembly method, so that the SERS detection sensitivity and the detection efficiency are effectively improved. The sampling opening is designed into a conical end sampling opening to form a capillary sampling mode; a to-be-detected liquid is directly drained to a to-be-detected zone by the capillary action; the effects caused by the dripping process are avoided; the chip comprises a plurality of detection zones, so that once sampling and multi-time detection can be achieved; and high detection repeatability is improved by determining the average value. The chip is relatively small in size, and convenient to carry, and can be used as a detection tool which is carried by a tester, and is relatively simple in preparation method, low in cost and suitable for multi-point parallel testing of the biological sample.

Description

Technical field [0001] The present invention involves the field of biochemical testing, chips, and spectral technology. Background technique [0002] The performance of non -destructive testing technology is not damaged and damaged the use of the objects of the test objects, which is of great significance in special biochemical testing.At present, detection methods commonly used in blood and biological samples include GC / LC-MS, DNA testing, immune testing, and micro-observation. These testing methods consume a large amount of samples and damage the sample.The existing non -destructive detection methods mainly adopt sound -light detection modes such as ultrasonic detection, ray detection, magnetic detection, sound launch detection, lidance holographic detection, and infrared detection.Testing, and Raman spectrometry technology will not cause chemistry and mechanical damage to the sample during the detection and analysis process, and it is not easy to produce light and thermal deco...

Claims

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Application Information

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IPC IPC(8): G01N21/65
Inventor 徐溢廖鑫赵华宙郑祥权温中泉
Owner CHONGQING UNIV
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