Product
Patsnap Eureka
For R&D, Patsnap Eureka makes reading and utilizing patents & technical documents easy.
Patsnap Eureka AIR
Designed for self-driven R&D workflows. Generate viable solutions, solve complex R&D challenges, empower your innovation with AI.
Patsnap Eureka Materials
Designed for material experts only. Revolutionize your material R&D, from search, analyze, to developing new materials.
Feature
TechResearch
Generate reliable direction feasibility study reports for your R&D in just a few steps.
TechSeek
Discover and master advanced knowledge NOW. Basics, ideas, possibilities, all at once.
TechMind
As an expert in R&D Theories, TechMind can generates customized viable solutions instantly.
TechRisk
Analyze your overall solution with one click, know your potential R&D risks in advance.
TechMonitor
Get weekly tech updates, stay abreast of the latest tech innovations and key insights.
Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
44results about How to "Strong detection specificity" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
Ratiometric fluorescent probe for bivalent copper ions, as well as preparation method and application of ratiometric fluorescent probe
InactiveCN104927867AStrong detection specificityImprove detection limitNanoopticsFluorescence/phosphorescenceModified carbonSio2 nanoparticle
The invention discloses a preparation method of a ratiometric fluorescent probe for bivalent copper ions. The preparation method comprises the following steps: 1) synthetizing cadmium telluride quantum dots: controlling the molar ratio of Cd2<+>:TGA:Te2<-> in a reaction product to be 1:2.5:0.5; 2) synthetizing amino-modified carbon quantum dots; 3) synthetizing blue-fluorescence carbon quantum dots doped with SiO2 nanoparticles: during synthesis, the volume ratio of the amino-modified carbon quantum dots obtained in the step 2), a chitosan solution of which the W / V is 0.5 percent, cyclohexane, Triton X-100, hexyl alcohol, ammonia water, TEOS and APTES is 5:1:750:177:180:5:6:3; 4) dispersing the cadmium telluride quantum dots obtained in the step 1) and the blue-fluorescence carbon quantum dots obtained in the step 3) in an MES buffer solution, adding a mixed solution of EDC and NHS, carrying out stirring, centrifugation and cleaning to obtain the probe. The probe prepared according to the method comprises nano particles containing the cadmium telluride quantum dots and the amino-modified carbon quantum dots, and has nucleus-satellite hybrid spherical structures. The invention further discloses application of the ratiometric fluorescent probe to detection of Cu2<+>. The ratiometric fluorescent probe has the advantages that the detection limit is very excellent, namely only 1.0*10<7>M; the preparation is simple; no expensive equipment is required.
Owner:SICHUAN AGRI UNIV
Fluorescence-quantitative PCR detection kit for gene transcription level of agasicles hygrophila actin
InactiveCN104962630AStrong detection specificityAccurate measurementMicrobiological testing/measurementDNA/RNA fragmentationFluorescenceActin genes
The invention relates to a kit for gene transcription level of agasicles hygrophila actin ( actin ), and belongs to the technical field of fluorescence-quantitative DNA expansion in vitro in the molecular biological detecting techniques. The invention firstly protects a detecting primer, and the sequence of the detecting primer is showed in SEQ. ID NO. 1 and SEQ. ID NO; secondly the fluorescence-quantitative PCR detection kit capable of detecting the gene transcription level of the agasicles hygrophila actin quantitatively is provided, the operation of the kit is simple, convenient and quick, and the kit is composed of SYBR Premix Ex Taq, primer mixture, a standard actin gene template and ultrapure water. The invention further protects the application, detecting the expresssion quantity of the agasicles hygrophila actin, of the kit. According to the kit for the gene transcription level of the agasicles hygrophila actin, the transcription level of the actin gene can be measured accurately, the high specificity is achieved, the expansion curve shows that the fluorescence signal value of the actin gene is in accord with a standard S-shaped curve, and a melting curve shows that the fluorescent quantitation has high detecting specificity.
Owner:INST OF PLANT PROTECTION FAAS
High-sensitive blood-plasma total homocysteine detection reagent box
InactiveCN1979155AStrong detection specificityHigh sensitivityComponent separationOther chemical processesTotal homocysteineHomocysteine testing
The invention relates to a high sensitive blood plasma homocysteine testing kit that includes sample tube with stabilizer, reference substance and quality control serum of different Hcy thickness, DL- homocystine-D8 internal standard solution, reducer and trichloroacetic acid albumen precipitation. The invention has strong specificity for sample testing, and high sensitivity. It could be accurately and rapidly used in Hcy research.
Owner:上海特敏生物医药科技有限公司
Hcy detecting method and detecting kit
ActiveCN103926248AFast measurementImprove accuracyAnalysis using chemical indicatorsMaterial analysis by observing effect on chemical indicatorPhysical chemistryBiology
The invention provides an Hcy concentration detecting method. According to the principle, oxidized form Hcy is changed into free Hcy, the free Hcy reacts with serine under the catalysis of CBS to generate L-cystathionine, and then the L-cystathionine generates Hcy, pyruvic acid and NH3 under the catalysis of CBL. The pyruvic acid generated through circular reaction can be detected through LDH and NADH, the rate of the NADH which is changed into NAD is in direct proportion to the content of the Hcy in a sample, and the content of the Hcy can be detected by detecting the lowering rate of NADH+ at the position of 340 nm. The invention further provides a high-sensitivity Hcy concentration detecting kit.
Owner:浙江夸克生物科技有限公司
Capillary electrophoresis method for detecting medications and poisons in blood and urine at the same time
InactiveCN1916602ASimple and efficient operationStrong detection specificityPreparing sample for investigationMaterial analysis by electric/magnetic meansSolid phasesDrug
A capillary cataphoresis method for detecting multiple medicinal toxic in blood and in urine simultaneously includes carrying out pretreatment by using similar solid phase to extract basic and acidic medicinal toxic, applying high efficiency of capillary cataphoresis detection and internal standard means of quantitative for enabling to analyze out 55 types of medicinal toxics contained possibly in one blood sample or in one urine sample within dozens of ten minutes.
Owner:上海市公安局刑事侦查总队
Test paper film based on nanogold and method for detecting lead ion by test paper film
ActiveCN104990918ANot prone to aggregationEasy to fixMaterial analysis by observing effect on chemical indicatorSulfate radicalsFilm base
The invention relates a test paper film based on nanogold and a method for detecting lead ion by the test paper film; a nano-titanium dioxide coating is coated on a basement membrane of the test paper film, and the nano-titanium dioxide coating is adhered with the nanogold and thiosulfuric acid root shell which are fixed by sulfydryl and amidogen. The invention also provides a method for detecting the lead ion in water by the test paper film based on the nanogold, the pre-treatment process for a sample to be detected is not needed, and large-scale equipment is not needed either; the method provided by the invention has the advantages of fast detection speed, low cost, strong anti-interference and low limit of detection; the detection lower limiting value of the lead ion can reach 5nM.
Owner:CHONGQING UNIV
Test paper strip for detecting Porcine Parvovirus and porcine encephalitis B virus antibody
InactiveCN101344524AStrong detection specificityHigh sensitivityMaterial analysisAcute viral encephalitisDisease
The invention discloses a test strip that is used for detecting antibodies of porcine parvovirus and porcine encephalitis B virus in one step, which comprises a supporting layer, an adsorptive layer and a protecting layer, and the adsorptive layer consists of a sample fiber layer, an adsorptive gold mark fiber layer, a fibrin membranous layer and a water absorptive material layer sequentially from a testing end; the adsorptive gold mark fiber layer adsorbs any one or two from antigen liquids of gold colloid marked SPA or gold colloid marked pure PPV and JEV; a detecting print and a contrast print are arranged on the fibrin membranous layer, the detecting print is printed with any one or two from the purified PPV and JEV antigen liquids, and the contrast print is printed with an IgG solution of ovine or rabbit anti-SPA or an IgG solution of any one or two viruses from ovine or rabbit anti-PPV and anti-JEV. The test strip of the invention has strong detecting specificity and high sensitivity, avoids additional equipment and reagents, can be operated by every person, and is particularly applicable to the quick specific antibody detection of porcine diseases on the spot.
Owner:HENAN ACAD OF AGRI SCI
DPO primer sequences for salmonella detection by using DPO-PCR method, and detection kit thereof
InactiveCN103320507AStrong detection specificityMicrobiological testing/measurementMicroorganism based processesSalmonellaPositive control
The invention relates to DPO primer sequences for salmonella detection by using a DPO-PCR method, and a diagnosis kit thereof, wherein the DPO primer sequences are that: SA-DPOF: 5'-TATTTTCCGGAAAACGTACCGCAIIIIIGCATTCCGC-3', and SA-DPOR: 5'-AGCCATACGGATAAACTGTGTTATIIIIIAGCGGAGGT-3'. The diagnosis kit contains the DPO primer pair, a positive control, a negative control, TaqDNAPolymerase and PCR reaction solutions. According to the invention, advantages of strong operability and accurate detection result are provided.
Owner:哈尔滨海关技术中心
Method for efficiently detecting 15 kinds of bile acid in blood
InactiveCN107356694ASolve the distance problemSave solutionComponent separationLiquid chromatography mass spectroscopyFilter paper
The invention provides a method for efficiently detecting 15 kinds of bile acid in blood. In the method, serum is directly preserved on a filter paper scrip, and through an efficient extraction process, extracted bile acid is directly injected on a liquid chromatography-mass spectroscopy; 15 kinds of the bile acid in blood can be efficiently detected in a high-throughput and high-repeatability mode, and the method has the wide application value.
Owner:HANGZHOU HEALTH BANK MEDICAL LAB CO LTD
Multifunctional magnetic DNA (deoxyribonucleic acid) nanosphere and preparation method and application thereof
ActiveCN106754894AEasy to separateStrong detection specificityOrganic active ingredientsMicrobiological testing/measurementDna templateBiology
The invention discloses a multifunctional magnetic DNA (deoxyribonucleic acid) nanosphere. The multifunctional magnetic DNA nanosphere comprises a DNA nanosphere combined with magnetic beads, and / or a DNA nanosphere combined with magnetic beads and containing double sulfur bonds. The multifunctional magnetic DNA nanosphere is characterized in that a phosphorylated linear DNA template single chain and a DNA primer single chain can complete the assembly of the nanosphere; in the nanosphere assembly process, the other DNA primer single chain is differently modified, and the phosphorylated linear DNA template single chain is subject to structural design, so as to assemble multiple types of functional groups in the nanosphere structure. The multifunctional magnetic DNA nanosphere has the advantages that the multifunctional magnetic DNA nanosphere can be used as a target transportation medicine carrier, so that the biological separation is easy; the DNA primer single chain of the multifunctional magnetic DNA nanosphere is modified by the double sulfur bonds, and the sulfhydryl compound in the cell can be detected.
Owner:LINYI UNIVERSITY
Structure-switching aptamer based highly-sensitive unmarked fluorescent detection method
InactiveCN102676656ALow costSuitable for practical application testingMicrobiological testing/measurementEnzyme digestionProtein secondary structure
The invention relates to a method for unmarked fluorescent detection of ions, protein and micromolecules. According to the method, a target in presence can be combined with aptamers to enable the aptamers to be folded into secondary structures, and the secondary structures are more difficult to degrade by nuclease than the unfolded aptamers, the quantity of the remaining aptamers after enzyme digestion is in direct proportion to the concentration of the target, the remaining aptamers can be quantitated by nucleic acid dye SYBR Gold, and the fluorescent dye can only used for dyeing nucleic acid chains and has no dyeing effect to a product obtained after the enzyme digestion. A fluophotometer is used for scanning samples and analyzing fluorescence intensity, and detection of the target is realized by means of change of the fluorescent signals. The higher the concentration of the target in the sample is, the stronger the fluorescent signals are. The method can be used for detecting various targets such as K+, thrombase and cocaine and high-throughput and multipath detection, and can realize low-cost, highly-sensitive, accurate and rapid detection of the targets.
Owner:CAPITAL NORMAL UNIVERSITY
Primer pair, probe and kit for detecting human VDR, GC, LRP5 and SLC30A8 gene polymorphism
InactiveCN108384844AStrong detection specificityImprove signal-to-noise ratioMicrobiological testing/measurementLRP5Nucleotide
The invention discloses a primer pair, a probe and a kit for detecting human VDR, GC, LRP5 and SLC30A8 gene polymorphism, and in particular a primer pair, a probe and a kit for detecting the gene polymorphism with the adoption of a multiple-asymmetrical fluorescent PCR probe melting curve method, wherein nucleotide sequences of upstream primers and downstream primers, which are used for amplifyinghuman VDR, GC, LRP5 and SLC30A8 genes, are sequentially shown SEQ ID NO:1-SEQ ID NO:8. The detection kit provided by the invention, which is free from complex experimental operations, is rapid and convenient; through complete closed-tube operations, cross pollution is prevented; and the kit is low in cost, high in sensitivity and strong in specificity, and the kit is applicable to large-scale promotion and application.
Owner:深圳鼎新融合科技有限公司
Synthesis of nickel-ion artificial antigen chelant and preparation of nickel monoclonal antibody
InactiveCN101597266AStrong detection specificityWeak cross-reactivitySerum albuminImmunoglobulinsAntigenBromine
The invention provides synthesis of a nickel-ion artificial antigen chelant and preparation of a nickel monoclonal antibody. A raw material 4-nitro-o-phenylenediamine is subjected to a series of reaction, and then is chemically synthesized into 6-bromine acetamide-2,3-(N,N,N',N'-tetraacethyl)dimethylamino-quinoxaline, BATDQ for short, which is taken as a difunctional chelant for synthesizing a nickel artificial antigen, and tetraacethyl and bromine acetamide structures of the BATDQ are chelated with nickel ions and carrier protein to obtain the artificial antigen; and then, the monoclonal antibody corresponding to nickel is obtained by immunizing BALB / c mice by the artificial antigen and applying technology such as cell fusion, cell clone and the like, and is used for detecting the residue of nickel in environment and food. The monoclonal antibody has the characteristics of quickness, sensitivity, low cost, and particular suitability for mass field quick detection; a preparation method for the nickel artificial antigen is simple, and has low cost; and the preparation technology for the monoclonal antibody is relative mature, and facilitates mass production, and the detection technology is simple, and is easy to promote.
Owner:ZHEJIANG FORESTRY UNIVERSITY
Organic fluorescent sensing material for detecting or distinguishing nerve poison and erosive poison as well as preparation method and application of organic fluorescent sensing material
ActiveCN111205857ALarge specific surface areaIncreased sensitivityOrganic chemistryFluorescence/phosphorescenceChemical compoundNerve agent
The invention provides an organic fluorescent sensing material which is obtained by co-assembling a compound represented by a formula (I) and a compound represented by a formula (II). According to theorganic fluorescent sensing material disclosed by the invention, two compounds with different structures are used for co-assembling, so that the defects that the detection is unstable when the compound represented by the formula (I) is singly used and the detection is insensitive when the compound represented by the formula (II) is singly used are overcome. The material obtained by co-assembly has the characteristics of large specific surface area, multiple surface pores and the like, and is beneficial to reducing the detection limit.
Owner:INST OF CHEM CHINESE ACAD OF SCI +1
Method for detecting impurity bromoacetic acid in cefathiamidine
PendingCN112083084AAvoid interferenceFast and efficient qualitative and quantitative detectionComponent separationAcetic acidFluid phase
The invention relates to a method for detecting impurity bromoacetic acid in cefathiamidine. According to the detection method, a high performance liquid chromatography-mass spectrometry method is adopted, and the conditions of high performance liquid chromatography are as follows: a mobile phase A: water; a mobile phase B: acetonitrile; the mobile phase A and the mobile phase B are adopted for gradient elution. According to the method, the detection limit of the bromoacetic acid in the cefathiamidine is low, the sensitivity is high, the bromoacetic acid in the cefathiamidine can be efficiently detected and controlled at the ng / ml level, and the method has important significance in quality control and medicinal safety of the cefathiamidine.
Owner:SICHUAN KELUN PHARMA RES INST CO LTD
Quick joint inspection test strip for Marek disease virus (MDV) and reticuloendotheliosis virus (REV)
ActiveCN103217527AStrong detection specificityHigh sensitivityMaterial analysisPolyclonal antibodiesBiomedical engineering
The invention discloses a test strip for quickly detecting Marek disease virus (MDV) and reticuloendotheliosis virus (REV) through a one-step method. The test strip comprises a water repellent supporting layer and an adsorbed layer adhered to the supporting layer, wherein the adsorbed layer is composed of a sample adsorption fiber layer, a gold-labeled antibody fiber layer, a cellulose membrane layer and a water accepting layer, which are spliced in sequence; controlled imprinting of anti-goat IgG or anti-mouse IgG and antibody detection imprinting containing anti-MDV and anti-REV are marked on the cellulose membrane layer; an antibody of the anti-MDV and anti-REV is a monoclonal antibody or a polyclonal antibody of the anti-MDV and anti-REV; and a mixed polyclonal antibody or a monoclonal antibody of the anti-MDV and anti-REV corresponding to the detection imprinting and marked by colloidal gold is adhered to the gold-labeled antibody fiber layer. The test strip is high in detection specificity, high in sensitivity, simple and convenient to operate, quick to detect and intuitive in result, so that the test strip is suitable for onsite quick joint inspection on MDV and REV and can be also applied to differential diagnosis on separate infection or mixed infection of the two viruses. The test strip has a wide application range and is convenient to generalize and apply.
Owner:HENAN ACAD OF AGRI SCI
Kit for simultaneously detecting multiple radiation sensitivity related SNP loci
ActiveCN112195229AStrong detection specificityHigh sensitivityMicrobiological testing/measurementDNA/RNA fragmentationMass spectrum analysisMolecular biology
The invention discloses a kit for simultaneously detecting multiple single nucleotide polymorphism (SNP) loci of multiple genes related to radiation sensitivity and application of the kit. According to the invention, by designing an amplification primer and a single-base extension primer of each locus, through multiple PCR and multiple single-base extension reactions, a specific product is analyzed by mass spectrometry, and the genotype of each locus is judged; and the kit has the characteristics of strong detection specificity, high sensitivity, rapidness, stability, high flux, low price, simplicity and convenience in operation and the like, and can be applied to screening of radiation sensitivity of scientific research and nuclear personnel.
Owner:中国人民解放军火箭军特色医学中心
Fluorogenic quantitative PCR detection kit for bovine ACTB gene transcription level
InactiveCN103290134AAccurate measurementStrong detection specificityMicrobiological testing/measurementFluorescenceGene
The invention discloses a fluorogenic quantitative PCR detection kit for bovine ACTB gene transcription level. The kit comprises 2XSYBR GREEN MIX, a primer mixed liquor, a standard ACTB genetic template and ultrapure water. The kit of the invention can be used to measure the ACTB gene transcription level accurately, and possesses high specificity (Figure 1). The results of an amplification curve show that ACTB fluorescence signal value accords with a standard "S" typed curve, and the results of a melting curve show that the fluorogenic quantitation possesses high detection specifity. [0]
Owner:LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
SARS-CoV-2 neutralizing antibody test strip and preparation method and kit thereof
ActiveCN113281523ARealize visual detectionStrong detection specificityBiological testingImmunoassaysAntigenNeutralising antibody
The invention relates to the technical field of biomedical detection, in particular to an SARS-CoV-2 neutralizing antibody test strip, and a preparation method thereof and a kit. The test strip comprises a bottom plate as well as a sample pad, a combination pad, a detection pad and a water absorption pad which are arranged on the bottom plate and are connected in sequence; and the sample pad is coated with SARS-CoV-2 ACE2, the combination pad is coated with a colloidal gold labeled SARS-CoV-2 S-RBD antigen, the detection pad is provided with a detection line and a control line, and the detection line is coated with the SARS-CoV-2 S-RBD antigen. The test strip does not need to use related instruments, is suitable for field detection of different scenes, is short in detection time and high in detection sensitivity, and further realizes field high-accuracy rapid detection.
Owner:南京申基医药科技有限公司
HPLC-ESI-MS/MS measuring method for simultaneously detecting 19 kinds of carbostyril medicaments
InactiveCN101315351BStrong detection specificityHigh sensitivityComponent separationTesting medicinal preparationsHplc esi msWater methanol
Owner:JIANGNAN UNIV
RT-HPLC detection method of piperazine ferulate related substances
ActiveCN113075336AEfficient determinationAchieve separationComponent separationAgainst vector-borne diseasesCarboxylic acidGradient elution
The invention relates to the technical field of pharmaceutical analysis, and discloses an RT-HPLC detection method of piperazine ferulate related substances, which comprises the following steps: preparing an analysis solution; using a reversed-phase chromatographic column, using acid or acid and carboxylate aqueous solution-organic phase mixed liquid as a mobile phase, and adopting a gradient elution method; and measuring the prepared analysis solution on a machine. The analysis method provided by the invention can effectively and accurately determine various related impurities in piperazine ferulate, thereby ensuring the controllable quality of the product.
Owner:成都亨达药业有限公司 +1
Glutaminase activity determination kit and method based on micromethod
InactiveCN109060791AEasy to prepareEliminate the influence of impuritiesMaterial analysis by observing effect on chemical indicatorColor/spectral properties measurementsGlutamineImpurity
The invention discloses a glutaminase activity determination kit and method based on micromethod. The kit provided by the invention comprises a first reagent: disodium hydrogen phosphate-citric acid buffer solution, a second reagent: glutamine aqueous solution, a third reagent: TCA aqueous solution, and a fourth reagent: ninhydrin powder. According to a determination principle provided by the invention, glutamine is catalyzed by GLS and is dissolved into L-glutamic acid and ammonia, and an increase rate of the glutamic acid is determined through utilization of a ninhydrin development method for the first time, thereby indirectly reflecting GLS enzymatic activity, so the kit and the method have originality. The employed reagent: ninhydrin is simple in preparation and is a common reagent ina laboratory. According to the determination method provided by the invention, through reasonable setting of comparison tubes, influence of impurities can be eliminated, and detection specifity is very high. The determination method provided by the invention is relatively good in repeatability. A variable coefficient (CV value) is within 2%. The determination method provided by the invention is applicable to various samples such as animals, plants, microorganisms and culture cells and is wide in application range.
Owner:SUZHOU COMIN BIOTECH
Method for detecting benzoic acid content in Kushen compound salicylic acid powder
InactiveCN105021752AAvoid decompositionImprove acidic environmentComponent separationBenzoic acidAlcohol
The invention discloses a method for detecting benzoic acid content in Kushen compound salicylic acid powder. The method uses high performance liquid chromatography and comprises the steps of: S1, preparation of a reference solution and a test solution comprises (1) preparation of the reference solution by taking a proper amount of analytically pure benzoic acid, accurately weighing, adding absolute ethyl alcohol to produce a solution as the reference solution containing 1.2 mg per 1 ml; (2) preparation of the test solution comprises taking 0.5g of Kushen compound salicylic acid powder, accurately weighing, adding 50ml into a volumetric flask, adding 40ml of absolute ethyl alcohol, sealing with plug, conducting ultrasonic treatment for 3-7 min, cooling, diluting with absolute ethyl alcohol to the mark, shaking evenly, filtering, and taking the filtrate as the test solution; and S2, detection: taking precisely 10 mul of the test solution and 10 mul of the reference solution, injecting into the liquid chromatograph, and measuring by using 0.03-0.07mol / L potassium dihydrogen phosphate solution-methanol as the mobile phase. The detection method provided by the invention is simple and easy to operation; the extraction of benzoic acid in the Kushen compound salicylic acid powder is thorough; and the detection result is accurate and reliable.
Owner:CHENGDU JIUZHITANG JINDING PHARMA
ELISA kit for capturing airborne allergens based on IgG3 antibody
InactiveCN108646025AStrong detection specificityHigh sensitivityBiological material analysisBiological testingEnzymeBiotin-binding proteins
The invention provides an ELISA kit for capturing airborne allergens based on an IgG3 antibody, and belongs to the technical field of detection of the airborne allergens. The ELISA kit includes the following reagents: a test plate coated with an anti-human IgG3 antibody; a washing buffer; a sample diluent; a standard or a positive quality control; a biotin-labeled airborne allergen preparation anda biotin-labeled anti-human IgG3 antibody preparation; an enzyme-labeled avidin solution; a substrate color developing solution; and a stop solution. The ELISA kit adopts the IgG3 to detect the airborne allergens, and the ELISA kit is an ELISA kit prepared based on a capture method, has the characteristics of high sensitivity and good reproducibility, and can process large quantities of samples at one time. The embodiment proves that the ELISA kit for capturing the airborne allergens based on the IgG3 antibody has a detection sensitivity of 1 ng / ml.
Owner:THE FIRST AFFILIATED HOSPITAL OF JINZHOU MEDICAL UNIV
Anti-H-FABP antibody labeled by colloidal selenium, detection card, and preparation method thereof
InactiveCN109283337ALow costStrong detection specificityImmunoglobulins against animals/humansDisease diagnosisAntibodyBioinformatics
The invention relates to an anti-H-FABP antibody labeled by colloidal selenium, a detection card, and a preparation method thereof. The anti-H-FABP antibody contains colloidal selenium particles and the anti-H-FABP antibody combined therewith. In the invention, the anti-H-FABP antibody labeled by colloidal selenium is used as a detection antibody on the H-FABP detection card, wherein the antibodyis low in cost; a test proves that the detection card is strong in detection specificity and short in detection time, so that the detection card can be extensively used for home self-detection of theH-FABP for myocardial infarction patients in early stage.
Owner:HENAN UNIVERSITY
Amphipathic polymer modified AuNPs (Au nanoparticles) organic mercury colorimetric detection method
ActiveCN109575915AHigh detection specificityAdjustable hydrophilic and hydrophobic propertiesMaterial nanotechnologyMaterial analysis by observing effect on chemical indicatorSeawaterEnvironmental chemistry
The invention discloses a preparation method of amphipathic polymer modified AuNPs and application of the amphipathic polymer modified AuNPs to organic mercury detection. According to the preparationmethod of the amphipathic polymer modified AuNPs, oleophilic fatty acyl chloride is applied to modifying hyperbranched polyethyleneimine (HPEI) to prepare an amphipathic hyperbranched polymer with a core-shell structure; the amphipathic hyperbranched polymer can be based on acid-alkali interaction to transfer chloroauric acid in aqueous phase into organic phase, and by means of the reducing capability of amido of the HPEI, can achieve in situ reduction to prepare high-stability AuNPs. The amphipathic polymer modified AuNPs can interact with organic mercury to form Au-Hg alloy to result in change of a system in color and ultraviolet-visible absorption spectrum and further to achieve high-selectivity detection of the organic mercury with a minimum detection limit as low as 1*10<-6> M. The amphipathic polymer modified AuNPs can gather the organic mercury through phase transfer, thereby, when applied to systems such as discrete-distribution and low-concentration seawater and soil, being high in sensitivity and selectivity of organic mercury detection.
Owner:LUDONG UNIVERSITY
Method for detecting content of chloride ions in body fluid through dendritic gold nanoparticles
PendingCN113418914AStrong oxidizingEtched thoroughlyMaterial analysis by observing effect on chemical indicatorNanoparticleChloride
The invention belongs to the technical field of chloride ion detection, and particularly relates to a method for detecting the content of chloride ions in body fluid through dendritic gold nanoparticles. The method comprises the following steps: preparing a dendritic gold nanoparticle solution, and preparing a paper-based gold nano material; mixing a sample to be detected with a nitric acid solution with the concentration of 8-16 mol / L, heating in a water bath environment with the temperature of 40-60 DEG C, adding the paper-based gold nano material, reacting for 30-40 minutes, and qualitatively detecting chloride ions when the paper-based gold nano material fades; and performing light intensity value analysis on the reacted paper-based gold nano-material through mage J software to obtain an optical signal of the paper-based gold nano-material so as to quantitatively detect the content of the chloride ions in the solution to be detected. The method for detecting the chlorine ion content in the body fluid by using the dendritic gold nanoparticles has the advantages of multi-color change, high detection sensitivity, simplicity in operation, high speed and the like.
Owner:湖南智享未来生物科技有限公司
Nano-gold test paper film and its application in chloride ion detection
ActiveCN108776133BResponsiveHigh detection sensitivityMaterial analysis by observing effect on chemical indicatorColor/spectral properties measurementsGold particlesChloride
The invention discloses a nano- gold test paper film. The nano-gold test paper film is formed by loading nano-gold particles on the surface of a PVDF film. The invention also discloses an applicationof the nano-gold test paper film in chloride ion detection based on the nano-gold test paper film. A method for detecting chloride ions comprises the following steps: S1, mixing a sample to be testedwith a nitric acid solution; S2, taking the nano-gold test paper film, adding the nano-gold test paper film to the mixed solution obtained in step S1, carrying out a reaction for 5-75 min, and qualitatively detecting chloride ions when the nano-gold test paper film fades; and S3, carrying out light intensity analysis on the reacted nano-gold test paper film by Image J software to obtain an opticalsignal in order to achieve quantitative detection of the concentration of chlorine ions in a solution to be tested. The nano-gold test paper film has the advantages of high detection sensitivity, high speed and simplicity operation when applied to the detection of chloride ions.
Owner:湖南智享未来生物科技有限公司
A kind of divalent copper ion ratio fluorescent probe and its preparation method and application
InactiveCN104927867BStrong detection specificityImprove detection limitNanoopticsFluorescence/phosphorescenceModified carbonSio2 nanoparticle
The invention discloses a preparation method of a ratiometric fluorescent probe for bivalent copper ions. The preparation method comprises the following steps: 1) synthetizing cadmium telluride quantum dots: controlling the molar ratio of Cd2<+>:TGA:Te2<-> in a reaction product to be 1:2.5:0.5; 2) synthetizing amino-modified carbon quantum dots; 3) synthetizing blue-fluorescence carbon quantum dots doped with SiO2 nanoparticles: during synthesis, the volume ratio of the amino-modified carbon quantum dots obtained in the step 2), a chitosan solution of which the W / V is 0.5 percent, cyclohexane, Triton X-100, hexyl alcohol, ammonia water, TEOS and APTES is 5:1:750:177:180:5:6:3; 4) dispersing the cadmium telluride quantum dots obtained in the step 1) and the blue-fluorescence carbon quantum dots obtained in the step 3) in an MES buffer solution, adding a mixed solution of EDC and NHS, carrying out stirring, centrifugation and cleaning to obtain the probe. The probe prepared according to the method comprises nano particles containing the cadmium telluride quantum dots and the amino-modified carbon quantum dots, and has nucleus-satellite hybrid spherical structures. The invention further discloses application of the ratiometric fluorescent probe to detection of Cu2<+>. The ratiometric fluorescent probe has the advantages that the detection limit is very excellent, namely only 1.0*10<7>M; the preparation is simple; no expensive equipment is required.
Owner:SICHUAN AGRI UNIV
Method for determining content of piperazine ferulate
PendingCN113063879AEfficient determinationEffective contentComponent separationIsocratic elutionPharmaceutical drug
The invention relates to the technical field of pharmaceutical analysis, and discloses an RT-HPLC (reverse transcription-high performance liquid chromatography) detection method for the content of piperazine ferulate, which comprises the following steps: preparing an analysis solution; using a reversed-phase chromatographic column, taking acid or acid and carboxylate aqueous solution-organic phase mixed liquid as a mobile phase, and adopting an isocratic elution method; and measuring the prepared analysis solution on a machine. The analysis method disclosed by the invention can be used for effectively and accurately determining the content of piperazine ferulate, so that the controllable quality of the product is ensured.
Owner:成都亨达药业有限公司 +1
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Patsnap Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com