44results about How to "Strong detection specificity" patented technology

The invention discloses a preparation method of a ratiometric fluorescent probe for bivalent copper ions. The preparation method comprises the following steps: 1) synthetizing cadmium telluride quantum dots: controlling the molar ratio of Cd2<+>:TGA:Te2<-> in a reaction product to be 1:2.5:0.5; 2) synthetizing amino-modified carbon quantum dots; 3) synthetizing blue-fluorescence carbon quantum dots doped with SiO2 nanoparticles: during synthesis, the volume ratio of the amino-modified carbon quantum dots obtained in the step 2), a chitosan solution of which the W/V is 0.5 percent, cyclohexane, Triton X-100, hexyl alcohol, ammonia water, TEOS and APTES is 5:1:750:177:180:5:6:3; 4) dispersing the cadmium telluride quantum dots obtained in the step 1) and the blue-fluorescence carbon quantum dots obtained in the step 3) in an MES buffer solution, adding a mixed solution of EDC and NHS, carrying out stirring, centrifugation and cleaning to obtain the probe. The probe prepared according to the method comprises nano particles containing the cadmium telluride quantum dots and the amino-modified carbon quantum dots, and has nucleus-satellite hybrid spherical structures. The invention further discloses application of the ratiometric fluorescent probe to detection of Cu2<+>. The ratiometric fluorescent probe has the advantages that the detection limit is very excellent, namely only 1.0*10<7>M; the preparation is simple; no expensive equipment is required.

The invention relates to a kit for gene transcription level of agasicles hygrophila actin ( actin ), and belongs to the technical field of fluorescence-quantitative DNA expansion in vitro in the molecular biological detecting techniques. The invention firstly protects a detecting primer, and the sequence of the detecting primer is showed in SEQ. ID NO. 1 and SEQ. ID NO; secondly the fluorescence-quantitative PCR detection kit capable of detecting the gene transcription level of the agasicles hygrophila actin quantitatively is provided, the operation of the kit is simple, convenient and quick, and the kit is composed of SYBR Premix Ex Taq, primer mixture, a standard actin gene template and ultrapure water. The invention further protects the application, detecting the expresssion quantity of the agasicles hygrophila actin, of the kit. According to the kit for the gene transcription level of the agasicles hygrophila actin, the transcription level of the actin gene can be measured accurately, the high specificity is achieved, the expansion curve shows that the fluorescence signal value of the actin gene is in accord with a standard S-shaped curve, and a melting curve shows that the fluorescent quantitation has high detecting specificity.

The invention provides an Hcy concentration detecting method. According to the principle, oxidized form Hcy is changed into free Hcy, the free Hcy reacts with serine under the catalysis of CBS to generate L-cystathionine, and then the L-cystathionine generates Hcy, pyruvic acid and NH3 under the catalysis of CBL. The pyruvic acid generated through circular reaction can be detected through LDH and NADH, the rate of the NADH which is changed into NAD is in direct proportion to the content of the Hcy in a sample, and the content of the Hcy can be detected by detecting the lowering rate of NADH+ at the position of 340 nm. The invention further provides a high-sensitivity Hcy concentration detecting kit.

A capillary cataphoresis method for detecting multiple medicinal toxic in blood and in urine simultaneously includes carrying out pretreatment by using similar solid phase to extract basic and acidic medicinal toxic, applying high efficiency of capillary cataphoresis detection and internal standard means of quantitative for enabling to analyze out 55 types of medicinal toxics contained possibly in one blood sample or in one urine sample within dozens of ten minutes.

The invention relates a test paper film based on nanogold and a method for detecting lead ion by the test paper film; a nano-titanium dioxide coating is coated on a basement membrane of the test paper film, and the nano-titanium dioxide coating is adhered with the nanogold and thiosulfuric acid root shell which are fixed by sulfydryl and amidogen. The invention also provides a method for detecting the lead ion in water by the test paper film based on the nanogold, the pre-treatment process for a sample to be detected is not needed, and large-scale equipment is not needed either; the method provided by the invention has the advantages of fast detection speed, low cost, strong anti-interference and low limit of detection; the detection lower limiting value of the lead ion can reach 5nM.

The invention discloses a test strip that is used for detecting antibodies of porcine parvovirus and porcine encephalitis B virus in one step, which comprises a supporting layer, an adsorptive layer and a protecting layer, and the adsorptive layer consists of a sample fiber layer, an adsorptive gold mark fiber layer, a fibrin membranous layer and a water absorptive material layer sequentially from a testing end; the adsorptive gold mark fiber layer adsorbs any one or two from antigen liquids of gold colloid marked SPA or gold colloid marked pure PPV and JEV; a detecting print and a contrast print are arranged on the fibrin membranous layer, the detecting print is printed with any one or two from the purified PPV and JEV antigen liquids, and the contrast print is printed with an IgG solution of ovine or rabbit anti-SPA or an IgG solution of any one or two viruses from ovine or rabbit anti-PPV and anti-JEV. The test strip of the invention has strong detecting specificity and high sensitivity, avoids additional equipment and reagents, can be operated by every person, and is particularly applicable to the quick specific antibody detection of porcine diseases on the spot.

The invention relates to DPO primer sequences for salmonella detection by using a DPO-PCR method, and a diagnosis kit thereof, wherein the DPO primer sequences are that: SA-DPOF: 5'-TATTTTCCGGAAAACGTACCGCAIIIIIGCATTCCGC-3', and SA-DPOR: 5'-AGCCATACGGATAAACTGTGTTATIIIIIAGCGGAGGT-3'. The diagnosis kit contains the DPO primer pair, a positive control, a negative control, TaqDNAPolymerase and PCR reaction solutions. According to the invention, advantages of strong operability and accurate detection result are provided.

The invention provides a method for efficiently detecting 15 kinds of bile acid in blood. In the method, serum is directly preserved on a filter paper scrip, and through an efficient extraction process, extracted bile acid is directly injected on a liquid chromatography-mass spectroscopy; 15 kinds of the bile acid in blood can be efficiently detected in a high-throughput and high-repeatability mode, and the method has the wide application value.

The invention discloses a multifunctional magnetic DNA (deoxyribonucleic acid) nanosphere. The multifunctional magnetic DNA nanosphere comprises a DNA nanosphere combined with magnetic beads, and/or a DNA nanosphere combined with magnetic beads and containing double sulfur bonds. The multifunctional magnetic DNA nanosphere is characterized in that a phosphorylated linear DNA template single chain and a DNA primer single chain can complete the assembly of the nanosphere; in the nanosphere assembly process, the other DNA primer single chain is differently modified, and the phosphorylated linear DNA template single chain is subject to structural design, so as to assemble multiple types of functional groups in the nanosphere structure. The multifunctional magnetic DNA nanosphere has the advantages that the multifunctional magnetic DNA nanosphere can be used as a target transportation medicine carrier, so that the biological separation is easy; the DNA primer single chain of the multifunctional magnetic DNA nanosphere is modified by the double sulfur bonds, and the sulfhydryl compound in the cell can be detected.

The invention relates to a method for unmarked fluorescent detection of ions, protein and micromolecules. According to the method, a target in presence can be combined with aptamers to enable the aptamers to be folded into secondary structures, and the secondary structures are more difficult to degrade by nuclease than the unfolded aptamers, the quantity of the remaining aptamers after enzyme digestion is in direct proportion to the concentration of the target, the remaining aptamers can be quantitated by nucleic acid dye SYBR Gold, and the fluorescent dye can only used for dyeing nucleic acid chains and has no dyeing effect to a product obtained after the enzyme digestion. A fluophotometer is used for scanning samples and analyzing fluorescence intensity, and detection of the target is realized by means of change of the fluorescent signals. The higher the concentration of the target in the sample is, the stronger the fluorescent signals are. The method can be used for detecting various targets such as K+, thrombase and cocaine and high-throughput and multipath detection, and can realize low-cost, highly-sensitive, accurate and rapid detection of the targets.

The invention discloses a primer pair, a probe and a kit for detecting human VDR, GC, LRP5 and SLC30A8 gene polymorphism, and in particular a primer pair, a probe and a kit for detecting the gene polymorphism with the adoption of a multiple-asymmetrical fluorescent PCR probe melting curve method, wherein nucleotide sequences of upstream primers and downstream primers, which are used for amplifyinghuman VDR, GC, LRP5 and SLC30A8 genes, are sequentially shown SEQ ID NO:1-SEQ ID NO:8. The detection kit provided by the invention, which is free from complex experimental operations, is rapid and convenient; through complete closed-tube operations, cross pollution is prevented; and the kit is low in cost, high in sensitivity and strong in specificity, and the kit is applicable to large-scale promotion and application.

The invention provides synthesis of a nickel-ion artificial antigen chelant and preparation of a nickel monoclonal antibody. A raw material 4-nitro-o-phenylenediamine is subjected to a series of reaction, and then is chemically synthesized into 6-bromine acetamide-2,3-(N,N,N',N'-tetraacethyl)dimethylamino-quinoxaline, BATDQ for short, which is taken as a difunctional chelant for synthesizing a nickel artificial antigen, and tetraacethyl and bromine acetamide structures of the BATDQ are chelated with nickel ions and carrier protein to obtain the artificial antigen; and then, the monoclonal antibody corresponding to nickel is obtained by immunizing BALB/c mice by the artificial antigen and applying technology such as cell fusion, cell clone and the like, and is used for detecting the residue of nickel in environment and food. The monoclonal antibody has the characteristics of quickness, sensitivity, low cost, and particular suitability for mass field quick detection; a preparation method for the nickel artificial antigen is simple, and has low cost; and the preparation technology for the monoclonal antibody is relative mature, and facilitates mass production, and the detection technology is simple, and is easy to promote.

The invention provides an organic fluorescent sensing material which is obtained by co-assembling a compound represented by a formula (I) and a compound represented by a formula (II). According to theorganic fluorescent sensing material disclosed by the invention, two compounds with different structures are used for co-assembling, so that the defects that the detection is unstable when the compound represented by the formula (I) is singly used and the detection is insensitive when the compound represented by the formula (II) is singly used are overcome. The material obtained by co-assembly has the characteristics of large specific surface area, multiple surface pores and the like, and is beneficial to reducing the detection limit.

The invention relates to a method for detecting impurity bromoacetic acid in cefathiamidine. According to the detection method, a high performance liquid chromatography-mass spectrometry method is adopted, and the conditions of high performance liquid chromatography are as follows: a mobile phase A: water; a mobile phase B: acetonitrile; the mobile phase A and the mobile phase B are adopted for gradient elution. According to the method, the detection limit of the bromoacetic acid in the cefathiamidine is low, the sensitivity is high, the bromoacetic acid in the cefathiamidine can be efficiently detected and controlled at the ng/ml level, and the method has important significance in quality control and medicinal safety of the cefathiamidine.

The invention discloses a test strip for quickly detecting Marek disease virus (MDV) and reticuloendotheliosis virus (REV) through a one-step method. The test strip comprises a water repellent supporting layer and an adsorbed layer adhered to the supporting layer, wherein the adsorbed layer is composed of a sample adsorption fiber layer, a gold-labeled antibody fiber layer, a cellulose membrane layer and a water accepting layer, which are spliced in sequence; controlled imprinting of anti-goat IgG or anti-mouse IgG and antibody detection imprinting containing anti-MDV and anti-REV are marked on the cellulose membrane layer; an antibody of the anti-MDV and anti-REV is a monoclonal antibody or a polyclonal antibody of the anti-MDV and anti-REV; and a mixed polyclonal antibody or a monoclonal antibody of the anti-MDV and anti-REV corresponding to the detection imprinting and marked by colloidal gold is adhered to the gold-labeled antibody fiber layer. The test strip is high in detection specificity, high in sensitivity, simple and convenient to operate, quick to detect and intuitive in result, so that the test strip is suitable for onsite quick joint inspection on MDV and REV and can be also applied to differential diagnosis on separate infection or mixed infection of the two viruses. The test strip has a wide application range and is convenient to generalize and apply.

The invention discloses a kit for simultaneously detecting multiple single nucleotide polymorphism (SNP) loci of multiple genes related to radiation sensitivity and application of the kit. According to the invention, by designing an amplification primer and a single-base extension primer of each locus, through multiple PCR and multiple single-base extension reactions, a specific product is analyzed by mass spectrometry, and the genotype of each locus is judged; and the kit has the characteristics of strong detection specificity, high sensitivity, rapidness, stability, high flux, low price, simplicity and convenience in operation and the like, and can be applied to screening of radiation sensitivity of scientific research and nuclear personnel.

The invention relates to the technical field of biomedical detection, in particular to an SARS-CoV-2 neutralizing antibody test strip, and a preparation method thereof and a kit. The test strip comprises a bottom plate as well as a sample pad, a combination pad, a detection pad and a water absorption pad which are arranged on the bottom plate and are connected in sequence; and the sample pad is coated with SARS-CoV-2 ACE2, the combination pad is coated with a colloidal gold labeled SARS-CoV-2 S-RBD antigen, the detection pad is provided with a detection line and a control line, and the detection line is coated with the SARS-CoV-2 S-RBD antigen. The test strip does not need to use related instruments, is suitable for field detection of different scenes, is short in detection time and high in detection sensitivity, and further realizes field high-accuracy rapid detection.

The invention relates to the technical field of pharmaceutical analysis, and discloses an RT-HPLC detection method of piperazine ferulate related substances, which comprises the following steps: preparing an analysis solution; using a reversed-phase chromatographic column, using acid or acid and carboxylate aqueous solution-organic phase mixed liquid as a mobile phase, and adopting a gradient elution method; and measuring the prepared analysis solution on a machine. The analysis method provided by the invention can effectively and accurately determine various related impurities in piperazine ferulate, thereby ensuring the controllable quality of the product.

The invention discloses a glutaminase activity determination kit and method based on micromethod. The kit provided by the invention comprises a first reagent: disodium hydrogen phosphate-citric acid buffer solution, a second reagent: glutamine aqueous solution, a third reagent: TCA aqueous solution, and a fourth reagent: ninhydrin powder. According to a determination principle provided by the invention, glutamine is catalyzed by GLS and is dissolved into L-glutamic acid and ammonia, and an increase rate of the glutamic acid is determined through utilization of a ninhydrin development method for the first time, thereby indirectly reflecting GLS enzymatic activity, so the kit and the method have originality. The employed reagent: ninhydrin is simple in preparation and is a common reagent ina laboratory. According to the determination method provided by the invention, through reasonable setting of comparison tubes, influence of impurities can be eliminated, and detection specifity is very high. The determination method provided by the invention is relatively good in repeatability. A variable coefficient (CV value) is within 2%. The determination method provided by the invention is applicable to various samples such as animals, plants, microorganisms and culture cells and is wide in application range.

The invention provides an ELISA kit for capturing airborne allergens based on an IgG3 antibody, and belongs to the technical field of detection of the airborne allergens. The ELISA kit includes the following reagents: a test plate coated with an anti-human IgG3 antibody; a washing buffer; a sample diluent; a standard or a positive quality control; a biotin-labeled airborne allergen preparation anda biotin-labeled anti-human IgG3 antibody preparation; an enzyme-labeled avidin solution; a substrate color developing solution; and a stop solution. The ELISA kit adopts the IgG3 to detect the airborne allergens, and the ELISA kit is an ELISA kit prepared based on a capture method, has the characteristics of high sensitivity and good reproducibility, and can process large quantities of samples at one time. The embodiment proves that the ELISA kit for capturing the airborne allergens based on the IgG3 antibody has a detection sensitivity of 1 ng/ml.

The invention relates to an anti-H-FABP antibody labeled by colloidal selenium, a detection card, and a preparation method thereof. The anti-H-FABP antibody contains colloidal selenium particles and the anti-H-FABP antibody combined therewith. In the invention, the anti-H-FABP antibody labeled by colloidal selenium is used as a detection antibody on the H-FABP detection card, wherein the antibodyis low in cost; a test proves that the detection card is strong in detection specificity and short in detection time, so that the detection card can be extensively used for home self-detection of theH-FABP for myocardial infarction patients in early stage.

The invention discloses a preparation method of amphipathic polymer modified AuNPs and application of the amphipathic polymer modified AuNPs to organic mercury detection. According to the preparationmethod of the amphipathic polymer modified AuNPs, oleophilic fatty acyl chloride is applied to modifying hyperbranched polyethyleneimine (HPEI) to prepare an amphipathic hyperbranched polymer with a core-shell structure; the amphipathic hyperbranched polymer can be based on acid-alkali interaction to transfer chloroauric acid in aqueous phase into organic phase, and by means of the reducing capability of amido of the HPEI, can achieve in situ reduction to prepare high-stability AuNPs. The amphipathic polymer modified AuNPs can interact with organic mercury to form Au-Hg alloy to result in change of a system in color and ultraviolet-visible absorption spectrum and further to achieve high-selectivity detection of the organic mercury with a minimum detection limit as low as 1*10<-6> M. The amphipathic polymer modified AuNPs can gather the organic mercury through phase transfer, thereby, when applied to systems such as discrete-distribution and low-concentration seawater and soil, being high in sensitivity and selectivity of organic mercury detection.

The invention relates to the technical field of pharmaceutical analysis, and discloses an RT-HPLC (reverse transcription-high performance liquid chromatography) detection method for the content of piperazine ferulate, which comprises the following steps: preparing an analysis solution; using a reversed-phase chromatographic column, taking acid or acid and carboxylate aqueous solution-organic phase mixed liquid as a mobile phase, and adopting an isocratic elution method; and measuring the prepared analysis solution on a machine. The analysis method disclosed by the invention can be used for effectively and accurately determining the content of piperazine ferulate, so that the controllable quality of the product is ensured.