44results about How to "Strong detection specificity" patented technology

A detection method for detecting HPLC-ESI-MS / MS of 19 species of quinolone drugs simultaneously belongs to the antibiotic drug analysis technology field. The detection method comprises three steps: sample treatment, liquid-phase separation and mass spectrometric detection, wherein the 19 species of quinolone antibiotics in a tissue fluid is subjected to positive ion MRM scan; norfloxacin-D5 is taken as the internal standard; C18 chromatographic columns are adopted for separation; water-methanol containing formic acid is taken as the mobile phase for gradient elution; and the eluent is subjected to mass spectrometric detection. The detection method is characterized in that the organic solvent extraction method is adopted for carrying out the sample pretreatment according to the practical situation of the sample and the different detection sensitivity requirements; norfloxacin-D5 is taken as the internal standard; the C18 chromatographic columns are adopted for separation; and the water-methanol containing formic acid is taken as the mobile phase for gradient elution. The pretreatment process of the method is rapid and convenient, the detection result is reliable and sensitive, and the method is suitable for the analysis and the detection of the quinolone drug residues in animal food.

The invention discloses a preparation method of a ratiometric fluorescent probe for bivalent copper ions. The preparation method comprises the following steps: 1) synthetizing cadmium telluride quantum dots: controlling the molar ratio of Cd2<+>:TGA:Te2<-> in a reaction product to be 1:2.5:0.5; 2) synthetizing amino-modified carbon quantum dots; 3) synthetizing blue-fluorescence carbon quantum dots doped with SiO2 nanoparticles: during synthesis, the volume ratio of the amino-modified carbon quantum dots obtained in the step 2), a chitosan solution of which the W / V is 0.5 percent, cyclohexane, Triton X-100, hexyl alcohol, ammonia water, TEOS and APTES is 5:1:750:177:180:5:6:3; 4) dispersing the cadmium telluride quantum dots obtained in the step 1) and the blue-fluorescence carbon quantum dots obtained in the step 3) in an MES buffer solution, adding a mixed solution of EDC and NHS, carrying out stirring, centrifugation and cleaning to obtain the probe. The probe prepared according to the method comprises nano particles containing the cadmium telluride quantum dots and the amino-modified carbon quantum dots, and has nucleus-satellite hybrid spherical structures. The invention further discloses application of the ratiometric fluorescent probe to detection of Cu2<+>. The ratiometric fluorescent probe has the advantages that the detection limit is very excellent, namely only 1.0*10<7>M; the preparation is simple; no expensive equipment is required.