Synthesis of nickel-ion artificial antigen chelant and preparation of nickel monoclonal antibody

A monoclonal antibody, artificial antigen technology, applied in genetic engineering, animal/human proteins, botanical equipment and methods, etc., can solve problems such as few types, no artificial antigens, hindering the research of heavy metal artificial antigens, etc.

Inactive Publication Date: 2009-12-09
ZHEJIANG FORESTRY UNIVERSITY
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Problems solved by technology

At present, there are very few types of bifunctional chelating agents, and the price is high, which hinders the research on the synthesis of heavy metal artificial antigens
The applicant had previously applied for the synthesis method of cadmium ion artificial antigen chelating agent, then introduced the synthesis of corresponding cadmium ion artificial antigen and monoclonal antibody, and applied the antibody to the detection

Method used

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  • Synthesis of nickel-ion artificial antigen chelant and preparation of nickel monoclonal antibody
  • Synthesis of nickel-ion artificial antigen chelant and preparation of nickel monoclonal antibody

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[0071] Specific implementation plan

[0072] The experimental reaction route of the present invention:

[0073]

[0074] The present invention utilizes the tetraacetic acid structure of BATDQ to form a stable chelate with heavy metal nickel ions. On the other hand, it utilizes its bromoacetamido structure to undergo condensation reaction with the sulfhydryl group on the protein under weakly alkaline conditions, thereby realizing The synthesis of heavy metal nickel artificial antigens laid the foundation for the enzyme immunoassay technology of heavy metal nickel. At the same time, the present invention is a beneficial supplement to the traditional heavy metal nickel ion detection technology, and its characteristics such as rapidity, high sensitivity, online operation and the like have become the new development direction of heavy metal detection.

[0075] Best embodiment:

[0076] The synthetic method of nickel ion artificial antigen chelating agent is as follows:

[0077] 1) Sy...

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Abstract

The invention provides synthesis of a nickel-ion artificial antigen chelant and preparation of a nickel monoclonal antibody. A raw material 4-nitro-o-phenylenediamine is subjected to a series of reaction, and then is chemically synthesized into 6-bromine acetamide-2,3-(N,N,N',N'-tetraacethyl)dimethylamino-quinoxaline, BATDQ for short, which is taken as a difunctional chelant for synthesizing a nickel artificial antigen, and tetraacethyl and bromine acetamide structures of the BATDQ are chelated with nickel ions and carrier protein to obtain the artificial antigen; and then, the monoclonal antibody corresponding to nickel is obtained by immunizing BALB/c mice by the artificial antigen and applying technology such as cell fusion, cell clone and the like, and is used for detecting the residue of nickel in environment and food. The monoclonal antibody has the characteristics of quickness, sensitivity, low cost, and particular suitability for mass field quick detection; a preparation method for the nickel artificial antigen is simple, and has low cost; and the preparation technology for the monoclonal antibody is relative mature, and facilitates mass production, and the detection technology is simple, and is easy to promote.

Description

technical field [0001] The invention relates to a new bifunctional chelating agent and the application of the chelating agent in the synthesis of heavy metal nickel ion artificial antigen and the preparation of nickel monoclonal antibody. Background technique [0002] The synthesis of artificial antigen is one of the key links in heavy metal immunoassay technology. It uses a bifunctional chelating agent to form a stable chelate (hapten molecule) with heavy metal ions, and then couples with a carrier protein to form a heavy metal-chelating agent- Chelates of carrier proteins (artificial antigens). At present, there are very few types of bifunctional chelating agents, and the price is high, which hinders the research on the synthesis of heavy metal artificial antigens. The applicant had previously applied for the synthesis method of cadmium ion artificial antigen chelating agent, then introduced the synthesis of corresponding cadmium ion artificial antigen and monoclonal anti...

Claims

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Application Information

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IPC IPC(8): C07D241/42C07K14/765C07K16/44C12N15/06C12P21/08G01N33/577G01N33/531G01N21/31
Inventor 郭明车晓青姚嘉赟刘力
Owner ZHEJIANG FORESTRY UNIVERSITY
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