Method for detecting content of chloride ions in body fluid through dendritic gold nanoparticles

A technology of gold nanoparticles and chloride ion content, applied in the field of chloride ion detection, can solve the problems of high cost, cumbersome detection, unsuitable application, etc., and achieve the effects of good selectivity, strong detection specificity, and good anti-interference ability.

Pending Publication Date: 2021-09-21
湖南智享未来生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The advantage of the titration method is that the measured results have high accuracy and are easy to operate; the disadvantages are low sensitivity, poor reproducibility, and large errors.
The advantages of ion chromatography are that it is suitable for determining the content of various ions in aqueous solution, with good precision and accuracy, easy operation, convenient sample pretreatment, and avoiding the harm of toxic chemicals to the human body, which can improve the analysis efficiency. Ion chromatography is expensive and not suitable for practical production applications
In summary, the existing methods have the disadvantages of cumbersome detection, low sensitivity, and high cost.

Method used

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  • Method for detecting content of chloride ions in body fluid through dendritic gold nanoparticles
  • Method for detecting content of chloride ions in body fluid through dendritic gold nanoparticles
  • Method for detecting content of chloride ions in body fluid through dendritic gold nanoparticles

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Embodiment 1

[0039] The present embodiment prepares dendritic gold nanomaterials:

[0040] A two-step method was employed to synthesize dendritic gold nanoparticles. First use the sodium citrate reduction method to synthesize 20nm spherical gold nanomaterials as seeds, and place a 250ml three-neck flask with 99mL ultrapure water in an oil bath to heat while stirring, and add 1mL after the water boils to a concentration of 1%. Sodium citrate solution, after a few minutes, the solution turns wine red, and then heated for half an hour, to prepare spherical gold nano-seeds;

[0041] Add the following solutions in turn to a 20 mL glass bottle with a cap filled with 5 mL of ultrapure water: 30 μL of 1% chloroauric acid solution, 31 μL of spherical nanoseed solution, 11 μL of 1% sodium citrate solution, 50 μL of hydroquinone solution with a molar concentration of 30 mmol / L. Shake quickly, and then stand for 20 minutes. When the solution turns blue, it means that the dendritic gold nanomaterials...

Embodiment 2

[0044] This embodiment prepares paper fund nanomaterials:

[0045] Use a puncher to punch polyvinylidene fluoride membrane (PVDF membrane) into paper pieces with a diameter of 5mm, take 10 pieces of paper and add them to 2mL dendritic gold nanoparticle solution and soak for 5 hours, and observe the color of the PVDF membrane. , use tweezers to take out the paper sheet, rinse it with ultrapure water, place it on a dry filter paper and let it air dry naturally to obtain a paper-based nanomaterial.

Embodiment 3

[0047] Chloride ion detection using paper-based nanomaterials:

[0048] Place the prepared paper fund nanomaterials in the following conditions: (1) 1.25mL concentrated nitric acid solution (16mol / L), (2) 1.25mL sodium chloride solution (1%), (3) 250μL sodium chloride (1%) and 1mL concentrated nitric acid (16mol / L) mixture. Place them at room temperature to react for 50 minutes, then take out the test paper sheet with tweezers, rinse it with ultra-pure water, place it on a dry filter paper to air dry and take pictures, observe the color of the gold nano test paper, and compare the color changes of the gold nano test paper under different conditions.

[0049] Such asfigure 1 Shown is the feasibility analysis diagram of dendritic gold nanomaterials for detecting sodium chloride; the figure (a) shows that when only concentrated nitric acid solution exists, the color of the PVDF membrane loaded with gold nanoparticles does not fade, and it still presents the original blue color. ...

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Abstract

The invention belongs to the technical field of chloride ion detection, and particularly relates to a method for detecting the content of chloride ions in body fluid through dendritic gold nanoparticles. The method comprises the following steps: preparing a dendritic gold nanoparticle solution, and preparing a paper-based gold nano material; mixing a sample to be detected with a nitric acid solution with the concentration of 8-16 mol / L, heating in a water bath environment with the temperature of 40-60 DEG C, adding the paper-based gold nano material, reacting for 30-40 minutes, and qualitatively detecting chloride ions when the paper-based gold nano material fades; and performing light intensity value analysis on the reacted paper-based gold nano-material through mage J software to obtain an optical signal of the paper-based gold nano-material so as to quantitatively detect the content of the chloride ions in the solution to be detected. The method for detecting the chlorine ion content in the body fluid by using the dendritic gold nanoparticles has the advantages of multi-color change, high detection sensitivity, simplicity in operation, high speed and the like.

Description

technical field [0001] The invention belongs to the technical field of chloride ion detection, and in particular relates to a method for detecting chloride ion content in body fluid with dendritic gold nanoparticles. Background technique [0002] Sodium chloride (NaCl) is an indispensable important substance in human life activities. It plays a vital role in the balance of water and osmotic pressure in the body, gastrointestinal digestive function, and acid-base balance in the body. Excessive intake of salt can lead to a series of diseases. At present, the most concerned diseases are hypertension, gastric cancer, asthma, osteoporosis and other diseases. Excessive intake of salt (sodium) has been listed as the eighth factor affecting human health. The World Health Organization recommends a daily salt intake of 5g per person. [0003] At present, the main detection methods of sodium chloride are: titration, ion chromatography and so on. The advantage of the titration method...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/7759
Inventor 许东李乐邓圣旺陈轶
Owner 湖南智享未来生物科技有限公司
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