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Kit for simultaneously detecting multiple radiation sensitivity related SNP loci

A kit and sensitive technology, applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve problems such as difficulty in establishing a radiation sensitivity evaluation system, and less interaction research, reaching the price Low cost, high sensitivity, and strong detection specificity

Active Publication Date: 2021-01-08
中国人民解放军火箭军特色医学中心
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

At present, domestic and foreign studies on the correlation between ionizing radiation and SNP mostly focus on the research on the radiosensitivity and radiation damage risk of single or several sites of a certain gene in cancer patients receiving radiotherapy. The reference standards for radiation damage are different, so there are few studies on the interaction between genes and between genes and radiation sensitivity, and it is difficult to establish a multi-gene and multi-locus radiation sensitivity evaluation system

Method used

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  • Kit for simultaneously detecting multiple radiation sensitivity related SNP loci
  • Kit for simultaneously detecting multiple radiation sensitivity related SNP loci
  • Kit for simultaneously detecting multiple radiation sensitivity related SNP loci

Examples

Experimental program
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Effect test

Embodiment 1

[0078] Embodiment 1, specific example

[0079] In this embodiment, 8 cases of whole blood samples were selected, respectively tested by the method of this study, and verified by the method of sanger sequencing, and the verification results were 100% consistent with the method.

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Abstract

The invention discloses a kit for simultaneously detecting multiple single nucleotide polymorphism (SNP) loci of multiple genes related to radiation sensitivity and application of the kit. According to the invention, by designing an amplification primer and a single-base extension primer of each locus, through multiple PCR and multiple single-base extension reactions, a specific product is analyzed by mass spectrometry, and the genotype of each locus is judged; and the kit has the characteristics of strong detection specificity, high sensitivity, rapidness, stability, high flux, low price, simplicity and convenience in operation and the like, and can be applied to screening of radiation sensitivity of scientific research and nuclear personnel.

Description

technical field [0001] The invention belongs to the technical field of gene detection, and relates to a kit for simultaneously detecting multiple single nucleotide polymorphism (SNP) sites of various genes related to radiation sensitivity and an application thereof. The present invention designs amplification primers and single-base extension primers for each site, performs multiple PCR and multiple single-base extension reactions, and then conducts mass spectrometry to analyze the specific products to determine the genotype of each site. Background technique [0002] After decades of hard work, my country's nuclear technology industry has entered a period of rapid development, and nuclear technology has been widely used in industry, agriculture, medical care, environmental protection, military and other fields. The most well-known application of civil nuclear technology is nuclear power plants. Nearly 50 nuclear power units have been built in my country, and dozens of nucle...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6872C12Q1/686C12N15/11
CPCC12Q1/6883C12Q1/6872C12Q1/686C12Q2600/16C12Q2600/156C12Q2537/143
Inventor 张阳东邓致荣江其生吕进郭宝石胡红芳苏璞殳晓强郭惠民
Owner 中国人民解放军火箭军特色医学中心
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